Self-assembled hemoglobin nanoparticles for improved oral photosensitizer delivery and oral photothermal therapy in vivo

Background:: The combination of photothermal therapy (PTT) and chemotherapy has proven to be a promising strategy for cancer treatment. Various nanomaterials have shown great potential in combination therapy, including gold, graphene oxide, iron oxide, and other nanoparticles. However, their undefinable toxicity in vivo greatly slowed down their development for clinical applications. Objective: The present work aimed to develop a multifunctional nanoparticle for chemo-photothermal therapy composed of acknowledged biocompatible materials. Methods: A novel biocompatible nanoparticle (HIT-NPs) was self-assembled through the intrinsic interaction between D-α-tocopherol Succinate (TOS), human serum albumin (HSA) and indocyanine green (ICG). Doxorubicin (DOX) was then loaded due to the ion pairing between DOX and TOS. The feasibility of combined chemo-photothermal therapy induced by DOX-loaded HIT-NPs was carefully evaluated. Results: In vitro, HIT-NPs showed no cytotoxicity on human normal liver cells (HL-7702 cells) but obvious killing effects murine breast cancer cells (4T1 cells). The combined chemo-photothermal therapeutic effect on 4T1 cells was successfully obtained. DOX-loaded HIT-NPs could effectively accumulate in 4T1 subcutaneous tumors after intravenous injection, and the tumor temperature rapidly increased under laser exposure, indicating the feasibility of PTT in vivo. Conclusion: The self-assembled HIT-NPs could provide a promising platform for combined chemo-photothermal cancer therapy with full biocompatibility.

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Self-assembled Nanoparticles of Polycationic b Rush and p Hotosensitizer for Synergistic Photothermal and Photodynamic Therapies Against Periodontitis

10.21203/rs.3.rs-832444/v1 ◽

2021 ◽

Author(s):

Enyu Shi ◽

Liya Bai ◽

Lujia Mao ◽

Hanping Wang ◽

Xiaoying Yang ◽

...

Keyword(s):

Self Assembly ◽

Photothermal Therapy ◽

Chronic Inflammatory Disease ◽

Bacterial Cells ◽

Antibacterial Effects ◽

Self Assembled ◽

Bacterial Cell Membrane ◽

Pass Through

Abstract Background: Periodontitis is a chronic inflammatory disease in oral cavity owing to bacterial infection. Photothermal therapy (PTT) and photodynamic therapy (PDT) have many advantages for antibacterial treatment. As an excellent photosensitizer, indocyanine green (ICG) shows prominent photothermal and photodynamic performances. However, it is difficult to pass through the negatively charged bacterial cell membrane, thus limits its antibacterial efficacy for periodontitis treatment.Results: In this work, we developed a nanosystem from self-assembly of ICG and polycationic brush for synergistic PTT and PDT against periodontitis. A star-shaped polycationic brush, poly(2-(dimethylamino)ethyl methacrylate) (sPDMA), was synthesized via atom transfer radical polymerization (ATRP) of DMA monomer from bromo-substituted β-cyclodextrin initiator (CD-Br). ICG was then self-assembled with sPDMA to form ICG-loaded sPDMA (sPDMA@ICG) nanoparticles (NPs), and the physicochemical properties of these NPs were characterized in detail. In vitro antibacterial effects of sPDMA@ICG NPs were evaluated in porphyromonas gingivalis (Pg), one of the recognized periodontitis pathogens, and in vivo anti-periodontitis effects of NPs were investigated in a rat periodontitis model. Benefiting from the unique brush-shaped architecture of sPDMA polycation, sPDMA@ICG NPs efficiently delivered ICG into the bacterial cells through promoting their adsorption and penetration abilities, and also exhibited effective antibacterial and anti-periodontitis actions via synergistic PTT and PDT both in vitro and in vivo.Conclusions: This work developed a promising nano-photosensitizer for synergistic PTT and PDT for antibacterial and periodontitis treatments in clinic.

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Boosting Chemodynamic Therapy by the Synergistic Effect of Co-Catalyze and Photothermal Effect Triggered by the Second Near-Infrared Light

Nano-Micro Letters ◽

10.1007/s40820-020-00516-z ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Songtao Zhang ◽

Longhai Jin ◽

Jianhua Liu ◽

Yang Liu ◽

Tianqi Zhang ◽

...

Keyword(s):

Cancer Therapy ◽

Bovine Serum Albumin ◽

Synergistic Effect ◽

Bovine Serum ◽

Photothermal Therapy ◽

Near Infrared ◽

Infrared Light ◽

Photothermal Effect ◽

Reaction Efficiency

AbstractIn spite of the tumor microenvironments responsive cancer therapy based on Fenton reaction (i.e., chemodynamic therapy, CDT) has been attracted more attentions in recent years, the limited Fenton reaction efficiency is the important obstacle to further application in clinic. Herein, we synthesized novel FeO/MoS2 nanocomposites modified by bovine serum albumin (FeO/MoS2-BSA) with boosted Fenton reaction efficiency by the synergistic effect of co-catalyze and photothermal effect of MoS2 nanosheets triggered by the second near-infrared (NIR II) light. In the tumor microenvironments, the MoS2 nanosheets not only can accelerate the conversion of Fe3+ ions to Fe2+ ions by Mo4+ ions on their surface to improve Fenton reaction efficiency, but also endow FeO/MoS2-BSA with good photothermal performances for photothermal-enhanced CDT and photothermal therapy (PTT). Consequently, benefiting from the synergetic-enhanced CDT/PTT, the tumors are eradicated completely in vivo. This work provides innovative synergistic strategy for constructing nanocomposites for highly efficient CDT.

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Core-Shell Pd@Au Nanoplates as Theranostic Agents for In-Vivo Photoacoustic Imaging, CT Imaging, and Photothermal Therapy

Advanced Materials ◽

10.1002/adma.201404013 ◽

2014 ◽

Vol 26 (48) ◽

pp. 8210-8216 ◽

Cited By ~ 270

Author(s):

Mei Chen ◽

Shaoheng Tang ◽

Zhide Guo ◽

Xiaoyong Wang ◽

Shiguang Mo ◽

...

Keyword(s):

Photothermal Therapy ◽

Photoacoustic Imaging ◽

Ct Imaging ◽

Core Shell ◽

Theranostic Agents

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Influence of the centrosome on the structure of nucleated microtubules.

The Journal of Cell Biology ◽

10.1083/jcb.100.4.1185 ◽

1985 ◽

Vol 100 (4) ◽

pp. 1185-1191 ◽

Cited By ~ 127

Author(s):

L Evans ◽

T Mitchison ◽

M Kirschner

Keyword(s):

Lattice Structure ◽

Neuroblastoma Cells ◽

Nucleation Sites ◽

Self Assembled ◽

Brain Microtubules

The capacity of the centrosome to influence the lattice structure of nucleated microtubules was studied in vitro. Brain microtubules self-assembled to give predominantly (98%) 14-protofilament microtubules. However, under exactly the same conditions of assembly they grew off of purified centrosomes from neuroblastoma cells to give mostly (82%) 13-protofilament microtubules. Thus, the nucleation sites on the centrosome constrained the microtubule lattice to yield the number of protofilaments usually found in vivo.

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