HYSTEROSCOPY BEFORE IN VITRO FERTILIZATION

In the last decade, success after in vitro fertilization process (IVF) has remained at a similar rate despite all the improvements implemented in the stimulation protocols and laboratory techniques. Hysteroscopy is a method becoming more widely used with patients after a failed IVF cycle, considering a large incidence of uterus cavum pathological states which have a negative impact on the favorable outcome. Numerous studies have provided different results on the IVF outcome with hysteroscopy performed prior to this treatment in cases with no uterus cavum pathology. The aim of the research was to examine the effect of both diagnostic and surgical hysteroscopy on the outcome of IVF. Hysteroscopy was performed with 74 patients 30 to 50 days prior to IVF and in 33 of them (group I) some pathological state was noticed, which was treated during the same procedure. The control group (group III) included 151 patients who had IVF performed with no prior hysteroscopy. There is no statistically significant difference in the rate of post hysteroscopy implantation between I and II group when compared to the control group (20.62% vs 23.28% vs 17.31%), nor in the rate of clinical pregnancies (45.45% vs 46.34% vs 34.44%). Following the correctional treatment of uterus cavum pathological states, implantation and pregnancy rates remain at a level comparable to hysteroscopically normal medical findings. Statistically significant higher pregnancy rate is present in group I after the first IVF cycle, compared to the next IVF in the same group and in comparison to the next IVF cycle in the control group (60.00% vs 27.91%, p<0.05). Hysteroscopy is a simple and safe method allowing nearly identical rate of clinical pregnancies after a surgical treatment of uterus cavum pathological states when compared to the control group, but statistically much higher pregnancy rate if the order of IVF procedure is being compared. In cases of normal ultrasound findings and negative hysteroscopical findings, performing hysteroscopy prior to IVF does not provide significantly better results. Therefore, its routine execution is not recommended.

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The effectiveness of collaborative infertility counseling (CIC) on pregnancy outcome in infertile women undergoing in vitro fertilization: A randomized controlled trial

10.21203/rs.2.14093/v1 ◽

2019 ◽

Author(s):

Mahboobeh Rasoulzadeh Bidgoli ◽

robab latifnejad roudsari ◽

ali montazeri

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Treatment Success ◽

Intervention Group ◽

Control Group ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

And Control

Abstract Background: Infertility is an emotional tension which influences the whole aspects of relationships in infertile couples. A main objective of infertility treatments is elevation of pregnancy rate. The present study aimed to examine the effect of collaborative counseling on pregnancy rate in infertile women, undergoing in vitro fertilization in Mashhad, Iran. Methods: In this clinical trial, 60 women with primary infertility were selected from an infertility research center and were randomly allocated into intervention (n=29) and control (n=31) groups. The intervention group received individual counseling, based on the collaborative reproductive healthcare model with collaboration of a midwife, a gynecologist and a clinical psychologist in five sessions during a two-month period. The control group received routine care. Positive pregnancy test was considered as a criterion of treatment success at the end of the study. Data were analyzed using statistical tests including independent samples t-test. Results: There was no significant difference in pregnancy rate between intervention and control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion: Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization

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The effectiveness of a collaborative infertility counseling (CIC) on pregnancy outcome in women undergoing in vitro fertilization: a randomized trial

BMC Pregnancy and Childbirth ◽

10.1186/s12884-020-03417-6 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Mahboobeh Rasoulzadeh Bidgoli ◽

Robab Latifnejad Roudsari ◽

Ali Montazeri

Keyword(s):

In Vitro Fertilization ◽

Randomized Trial ◽

Pregnancy Rate ◽

Intervention Group ◽

Fertility Treatment ◽

Control Group ◽

Vitro Fertilization ◽

Group Randomized Trial ◽

Significant Difference

Abstract Background The optimal objective of infertility treatments is to increase pregnancy rate. The aim of this study was to assess the effectiveness of a collaborative counseling program on pregnancy rate in women undergoing in vitro treatment. Methods This was a parallel group randomized trial on a sample of 60 women attending to an infertility research center affiliated to Mashhad University of Medical Sciences for fertility treatment. Women were randomly assigned to an intervention or a control group. Then, a five-session program offered to the intervention group while the control group received nothing expect the usual care. The primary outcome for the study was positive pregnancy test at the end of study. Statistical analyses including independent samples t-test were performed to explore the data. Results The outcome analysis showed that there were no significant differences in pregnancy rate between the intervention and the control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization. Trial registration IRCT201110267915N1. Registered 2014.07.25-Retrospectively registered (http://en.irct.ir/trial/8359).

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Comparison of short and long co-incubation time of gametes for in vitro fertilization

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20194548 ◽

2019 ◽

Vol 8 (11) ◽

pp. 4366

Author(s):

Pallop Pongsuthirak

Keyword(s):

In Vitro Fertilization ◽

Incubation Time ◽

Pregnancy Outcomes ◽

Fertilization Failure ◽

Vitro Fertilization ◽

Group I ◽

Significant Difference ◽

Total Fertilization Failure ◽

Rescue Icsi

Background: The short and long co-incubation time of gametes for in vitro fertilization are still debatable issues. This study aims to investigate the effects of short and long co-incubation time of gametes on fertilization, polyspermy, embryonic developmental potential, and clinical outcomes.Methods: Sixty-five patients undergoing IVF treatment were invited to participate in the study between May 2017 and March 2019. Ovarian hyperstimulation was prescribed and oocytes were obtained by trans-vaginal aspiration under ultrasound guidance. Sibling oocytes were randomly allocated to short co-incubation for 4 hours (Group I) in 352 oocytes and long co-incubation for 16-18 hours in 363 oocytes (Group II). Rescue ICSI was carried out if total fertilization failure was documented. Fertilization, embryonic development, and pregnancy outcomes were determined.Results: No significant differences between short and long co-incubation were found in fertilization, polyspermy, cleavage, blastocyst, implantation, clinical pregnancy, and live birth rates.Conclusions: The present study showed that short co-incubation of gametes had no significant difference in fertilization, polyspermy, embryo development, and pregnancy outcomes when compared to long co-incubation. The short co-incubation with early cumulus cell removal and rescue ICSI may have the potential to help a couple who had total fertilization failure.

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15 QUANTIFICATION OF BULL SPERM TRAITS AS ASSESSED BY COMPUTER-ASSISTED SEMEN ANALYSIS AND THE RELATIONSHIP TO PREGNANCY RATE FOLLOWING CONTROLLED BREEDING

Reproduction Fertility and Development ◽

10.1071/rdv29n1ab15 ◽

2017 ◽

Vol 29 (1) ◽

pp. 115

Author(s):

M. L. Mphaphathi ◽

M. M. Seshoka ◽

F. V. Ramukhithi ◽

Z. C. Raphalalani ◽

T. R. Netshirovha ◽

...

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Semen Analysis ◽

Computer Assisted ◽

Vitro Fertilization ◽

Significant Difference ◽

Bull Sperm ◽

Sperm Traits

The bull’s contribution through artificial insemination to reproductive efficiency is of great biological importance. The objectives were (1) to compare the oestrous synchronization response of Bonsmara and Nguni cows; and (2) to find the relationship between cow’s conception rate (in vivo and in vitro fertilization) and bull sperm motility rate assessed by computer-assisted semen analysis (CASA) following AI. For the in vivo sperm fertility test, 100 Bonsmara and 482 Nguni cows were randomly selected and subjected to oestrous synchronization protocol and AI with frozen–thawed assessed semen by CASA before AI. Briefly at Day 0, cows were inserted with an intravaginal CIDR® (1.9 g), which was removed on Day 7. Prostaglandin was then administered (2 mL) on Day 8 and a heatmount detector was placed on the hindquarter of each cow. For the in vitro sperm fertility test, collected oocytes from slaughterhouse were in vitro matured (n = 360) and in vitro fertilized (sperm/mL) in 100-µL droplets (final volume) of BO-IVF medium per treatment bulls (Bonsmara or Nguni bull). The frozen/thawed semen straws of Bonsmara and Nguni bulls were randomly selected and used under the same IVF conditions. The thawed bull’s sperm characteristics were examined by CASA before in vitro fertilization. Data were analysed using ANOVA. Treatment means were compared using the Fisher’s protected least significant difference t-test. There was no significant difference in oestrous response for the Bonsmara (83.0%) and Nguni (90.8%) cows, respectively. The Bonsmara cows recorded a significantly higher pregnancy rate (59.0%) compared with the Nguni (37.1%) cows (P < 0.05). Sperm traits such as total motility (TM), progressive motility and rapid were found to be positively correlated with conception rate (r = 0.06, 0.03, and 0.08, respectively; P < 0.01), although correlations were low. There was no difference in the average frozen–thawed sperm TM rate of Nguni (92.2%) and Bonsmara (81.0%). There was a lower fertilization rate following IVF with Bonsmara and Nguni bull sperm. In conclusion, Nguni cows had similar oestrous response as Bonsmara cows. The sperm traits from Bonsmara and Nguni bulls were found to be related to in vivo conception and in vitro fertilization rate when sperm cells were assessed by CASA technology. However, the pregnancy rate was lower in Nguni cows.

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The Impact of Noncavity-Distorting Intramural Fibroids on the Efficacy of In Vitro Fertilization-Embryo Transfer: An Updated Meta-Analysis

BioMed Research International ◽

10.1155/2018/8924703 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13

Author(s):

Xiaodan Wang ◽

Li Chen ◽

Hengyu Wang ◽

Qin Li ◽

Xiru Liu ◽

...

Keyword(s):

In Vitro Fertilization ◽

Pregnancy Rate ◽

Meta Analysis ◽

Control Group ◽

Study Group ◽

Vitro Fertilization ◽

The Impact ◽

Intramural Fibroids ◽

Ivf Et

Aim. To address the impact of noncavity-distorting intramural fibroids on the efficacy of in vitro fertilization-embryo transfer (IVF-ET) outcomes. Methods. The PubMed, Web of Science, Embase, Cochrane Library, and China National Knowledge Infrastructure were searched systematically. A meta-analysis was performed based on comparative or cohort studies that explored the impact of noncavity-distorting intramural fibroids on the efficacy of IVF-ET treatment. The IVE-ET outcomes of study group (women with noncavity-distorting intramural fibroids) and control group (women without fibroids) were compared, including live birth rate (LBR), clinical pregnancy rate (cPR), implantation rate (IR) , miscarriage rate (MR), and ectopic pregnancy rate (ePR). Results. A total of 28 studies involving 9189 IVF cycles were included. Our meta-analysis showed a significant reduction of LBR in the study group compared to control group (RR = 0.82, 95% CI: 0.73-0.92, and P = 0.005). In addition, it indicated that study group had a significant reduction in cPR (RR = 0.86, 95% CI: 0.80-0.93, P = 0.0001) and IR (RR = 0.90, 95% CI: 0813-1.00, P = 0.04) and have a significantly increase in MR (RR = 1.27, 95% CI: 1.08-1.50, and P = 0.004) compared with control group. Conclusions. The present evidence suggests that noncavity-distorting intramural fibroids would significantly reduce the IR, cRP, and LBR and significantly increase the MR after IVF treatment, but it would not significantly increase the ePR.

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Effect of intrauterine granulocyte-colony stimulating factor administration on in vitro fertilization outcome in women with moderate-to-severe endometriosis: An RCT

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i8.9621 ◽

2021 ◽

pp. 733-740

Author(s):

Ladan Kashani ◽

Ashraf Moini ◽

Tayebeh Esfidani ◽

Nazila Yamini ◽

Shima Mohiti

Keyword(s):

In Vitro Fertilization ◽

Clinical Pregnancy ◽

Control Group ◽

Granulocyte Colony Stimulating Factor ◽

Colony Stimulating Factor ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

Stimulating Factor

Background: Nearly 25-50% of infertile women have endometriosis. There are reports of disorders in the expression of granulocyte colony-stimulating factor (G-CSF) receptors in women with endometriosis. Objective: To examine the effect of intrauterine administration of G-CSF in in vitro fertilization (IVF) cycles on the fertility rate of infertile women with moderate-to-severe endometriosis. Materials and Methods: This clinical trial was conducted on 66 infertile women with moderate-to-severe endometriosis, undergoing IVF and intracytoplasmic sperm injection (ICSI). The participants were allocated into two groups via simple randomization: the G-CSF (n = 27) and control (n = 39) groups. In the G-CSF intervention group, on the oocyte pick-up day, immediately after an ovarian puncture, 300 μg of G-CSF was injected through a transcervical catheter under abdominal ultrasound guide to visualize flushing into the uterine cavity. Women in the control group received no intervention. The two groups were evaluated for clinical pregnancy. Results: No significant difference was noted in the demographic characteristics of the two groups. The rate of clinical pregnancy was 28.2% in the control group and 25.9% in the G-CSF group, indicating no significant difference (p = 0.83). Conclusion: The results showed that the intrauterine injection of G-CSF had no effects on pregnancy in women with stage-3/4 endometriosis undergoing IVF. Key words: G-CSF, In vitro fertilization, Endometriosis, Pregnancy.

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Pregnancy benefit of acupuncture on in vitro fertilization: A systematic review and meta-analysis

10.22541/au.163257866.66473667/v1 ◽

2021 ◽

Author(s):

Shiyan Yan ◽

Xiaoyu Liu ◽

Zhenqing Pu ◽

Yuhan Lin ◽

Zhiyi Xiong ◽

...

Keyword(s):

In Vitro Fertilization ◽

Random Effect ◽

Random Effect Model ◽

Live Birth Rate ◽

Current Evidence ◽

Control Group ◽

Vitro Fertilization ◽

Statistical Heterogeneity ◽

Significant Difference

Background: The evidence of acupuncture for in vitro fertilization (IVF) remains debatable. Objectives: To evaluate the efficacy of acupuncture on embryo transfer in women undergoing IVF. Search Strategy: PubMed, MEDLINE, Web of Science, EMBASE, CENTRAL, Wanfang, Chinese National Knowledge Infrastructure, VIP were searched up to 6 September 2021. Selection Criteria: Randomized controlled trials (RCTs) of acupuncture evaluating IVF’s effects were included. Data Collection and Analysis: Clinical pregnancy rate (CPR) and live birth rate (LBR) were quantitatively summarized by the random-effect model. Variations in pooled estimates were examined by subgroup analyses and I2 was measured to quantify statistical heterogeneity. Main Results: Forty-nine eligible RCTs representing 9422 women undergoing IVF for pregnant success were identified. Pooled CPR and LBR showed a significant difference between acupuncture and control groups (odds ratio [OR]=1.65, 95% confidence interval [CI]: 1.45 to 1.88; OR=1.34, 95% CI: 1.08 to 1.66). Subgroup analysis showed a benefit of traditional acupuncture for women aged < 35 years (CPR: OR=1.57, 95% CI: 1.23 to 2.03; LBR: OR=1.34, 95% CI:1.23 to 1.48), treatment with  3 sessions (CPR: OR=2.37, 95% CI: 1.46 to 3.85; LBR: OR=1.99, 95% CI:1.52 to 2.60), and lower baseline control group rates of CPR (CPR: OR=2.14, 95% CI: 1.22 to 2.13; LBR: OR=2.23, 95% CI:1.07 to 4.62). Heterogeneity across studies were found (CPR: I2=47.4, p <0.001; LBR: I2=62.9, p <0.001). Conclusions: Although benefits of acupuncture in CPR and LBR among IVF women existed, no robust associations were found and which might be limited by the heterogeneity of current evidence

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The effect of acupuncture on the day of embryo transfer on the in vitro fertilization outcomes: An RCT

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v18i3.6719 ◽

2020 ◽

Author(s):

Alamtaj Samsami Dehghani ◽

Kaynoosh Homayouni ◽

Zahra Kanannejad ◽

Zeinab Kanannejad

Keyword(s):

In Vitro Fertilization ◽

Embryo Transfer ◽

Pregnancy Rates ◽

Control Group ◽

Vitro Fertilization ◽

Significant Difference ◽

Before And After ◽

Mother And Child ◽

Child Hospital

Background: Acupuncture is an adjunct therapy to support infertile women received in vitro fertilization (IVF) treatment; however, the efficacy of this approach needs more evaluation. Objective: This randomized clinical trial (RCT) study aimed to evaluate the influence of acupuncture on reproductive outcomes in women undergoing IVF treatment. Materials and Methods: The study was carried out on 186 participants who had undergone IVF treatment in the Mother and Child Hospital between September 2015 and February 2016. Subjects were randomly divided into three groups: Acupuncture 25 min before embryo transfer (ET) (ACU1 group, n = 62), acupuncture 25 min before and after ET (ACU2 group, n = 62), and ET without acupuncture (control group, n = 62). Pregnancy rates (biochemical, clinical, and ongoing) were evaluated and compared between groups. Results: There were significant differences between the ACU1 group and the control group regarding biochemical (p = 0.005), clinical (p = 0.006), and ongoing (p = 0.007) pregnancies. Also, our results showed that two-session acupuncture (ACU2) lead to a significant reduction in frequency of biochemical (p = 0.002), clinical (p = 0.003), and ongoing (p = 0.01) pregnancy rates when compared to the one-session acupuncture (ACU1). No significant difference was found between the ACU2 and control groups regarding the aforementioned terms (p = 0.50). Conclusion: Acupuncture 25 min before ET significantly increased the IVF outcomes in women undergoing IVF compared with no acupuncture. Repeating acupuncture 25 min after ET did not improve the IVF outcome. Key words: Acupuncture, Embryo transfer, In vitro fertilization, Pregnancy rate.

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The clinical outcome of Dienogest treatment followed by in vitro fertilization and embryo transfer in infertile women with endometriosis

Journal of Ovarian Research ◽

10.1186/s13048-019-0597-y ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 3

Author(s):

Hiroshi Tamura ◽

Hiroaki Yoshida ◽

Hiroyuki Kikuchi ◽

Mai Josaki ◽

Yumiko Mihara ◽

...

Keyword(s):

Oxidative Stress ◽

In Vitro Fertilization ◽

Clinical Outcome ◽

Control Group ◽

Vitro Fertilization ◽

Infertile Women ◽

Significant Difference ◽

Ivf Et ◽

Follicular Fluids

Abstract Background Endometriosis is considered to be the most intractable cause of female infertility. Administering any type of treatment for endometriosis before in vitro fertilization and embryo transfer (IVF-ET) is an important strategy for improving the IVF-ET outcomes for infertile women with endometriosis. In fact, treatment with a gonadotropin-releasing hormone (GnRH) agonist just before IVF-ET has been reported to improve the clinical outcome in endometriosis patients. However, the benefit of Dienogest (DNG), a synthetic progestin, treatment just before IVF-ET remains unclear. Methods Sixty-eight infertile women with Stage III or IV endometriosis (ovarian endometrial cyst < 4 cm) were recruited for this study. The subjects were divided into 2 groups: a DNG group (n = 33) and a control group (n = 35). DNG was administered orally every day for 12 weeks prior to the conventional IVF-ET cycle in the DNG group. Standard controlled ovarian hyperstimulation with the GnRH agonist long protocol was performed in the control group. The numbers of mature follicles and retrieved oocytes, fertilization rates, implantation rates, and clinical pregnancy rate were compared between the two groups. In addition, the concentrations of inflammatory cytokines, oxidative stress markers, and antioxidants in follicular fluids were also measured. Results The numbers of growing follicles, retrieved oocytes, fertilized oocytes, and blastocysts were significantly lower in the DNG group than in the control group. The fertilization and blastocyst rates were also lower in the DNG group than in the control group. Although there was no significant difference in the implantation rate between the groups, the cumulative pregnancy rate and live birth rate were lower in the DNG group than in the control group. There was no significant difference in the abortion rate. Our results failed to show that DNG reduces the inflammatory cytokine levels and oxidative stress in follicular fluids. Conclusions Administering DNG treatment just before IVF-ET did not provide any benefits to improve the clinical outcomes for infertile women with endometriosis.

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Does Acupuncture Have Any Benefit in the IVF Treatment of Couples with Unexplained Infertility; A Prospective Randomized Controlled Trial

Gynecology Obstetrics and Reproductive Medicine ◽

10.21613/gorm.2019.872 ◽

2019 ◽

pp. 1

Author(s):

Songul Ece Gonen ◽

Pınar Kadirogullari ◽

Kerem Doga Seckin ◽

Bunyamin Borekci

Keyword(s):

In Vitro Fertilization ◽

Embryo Transfer ◽

Controlled Trial ◽

Unexplained Infertility ◽

Controlled Study ◽

Control Group ◽

Study Group ◽

Vitro Fertilization ◽

Significant Difference

Objective: We aimed to investigate the effect of acupuncture performed as an adjuvant therapy to in vitro fertilization, on pregnancy rates by performing acupuncture on the day of embryo transfer, one hour before the procedure and one hour after the procedure.Study Design: In this open-label randomized prospective controlled study, 60 unexplained infertility patients enrolled for in vitro fertilization treatment were randomized by a computer-based number generator for acupuncture performance and no-treatment. All patients received rFSH (Gonal-F®) for ovulation induction and the antagonist Cetrorelix (Cetrotide®) to prevent premature ovulation. Ovulation was triggered by using hCG (Ovitrelle®) and egg collection was done after 36-48 hours. In the study group, acupuncture was performed by intradermal needling, bilaterally with a sterile needle, both one hour before and one hour after the embryo transfer. Acupuncture was not performed on the control group. The results of the treatment were evaluated 12 days after embryo transfer.Results: Thirty patients were randomized into the study group and 30 patients were randomly placed in the control group. Although there was no statistically significant difference between groups in terms of β-hCG positivity, it was 43.3% in the study group and 36.7% in the control group (p>0.05).Conclusion: The success rate of in vitro fertilization was higher in terms of pregnancy rate in the patient group who received acupuncture procedure as well, but the difference was not statistically significant. This may be due to the type of acupuncture procedure selected or insufficient sample size. As a result, further studies involving larger numbers of participants and using different acupuncture techniques are needed.

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