Degradation of Household detergents using fungi isolated from freshwater ecosystem

2016 ◽  
Vol 3 (9) ◽  
pp. 120-123
2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Catherine M. Febria ◽  
Maggie Bayfield ◽  
Kathryn E. Collins ◽  
Hayley S. Devlin ◽  
Brandon C. Goeller ◽  
...  

In Aotearoa New Zealand, agricultural land-use intensification and decline in freshwater ecosystem integrity pose complex challenges for science and society. Despite riparian management programmes across the country, there is frustration over a lack in widespread uptake, upfront financial costs, possible loss in income, obstructive legislation and delays in ecological recovery. Thus, social, economic and institutional barriers exist when implementing and assessing agricultural freshwater restoration. Partnerships are essential to overcome such barriers by identifying and promoting co-benefits that result in amplifying individual efforts among stakeholder groups into coordinated, large-scale change. Here, we describe how initial progress by a sole farming family at the Silverstream in the Canterbury region, South Island, New Zealand, was used as a catalyst for change by the Canterbury Waterway Rehabilitation Experiment, a university-led restoration research project. Partners included farmers, researchers, government, industry, treaty partners (Indigenous rights-holders) and practitioners. Local capacity and capability was strengthened with practitioner groups, schools and the wider community. With partnerships in place, co-benefits included lowered costs involved with large-scale actions (e.g., earth moving), reduced pressure on individual farmers to undertake large-scale change (e.g., increased participation and engagement), while also legitimising the social contracts for farmers, scientists, government and industry to engage in farming and freshwater management. We describe contributions and benefits generated from the project and describe iterative actions that together built trust, leveraged and aligned opportunities. These actions were scaled from a single farm to multiple catchments nationally.


Amylase ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 38-49
Author(s):  
Connie Pontoppidan ◽  
Svend G. Kaasgaard ◽  
Carsten P. Sønksen ◽  
Carsten Andersen ◽  
Birte Svensson

Abstract The industrial thermostable Bacillus licheniformis α-amylase (BLA) has wide applications, including in household detergents, and efforts to improve its performance are continuously ongoing. BLA during the industrial production is deamidated and glycated resulting in multiple forms with different isoelectric points. Forty modified positions were identified by tandem mass spectrometric peptide mapping of BLA forms separated by isoelectric focusing. These modified 12 asparagine, 9 glutamine, 8 arginine and 11 lysine residues are mostly situated on the enzyme surface and several belong to regions involved in stability, activity and carbohydrate binding. Eight residues presumed to interact with starch at the active site and surface binding sites (SBSs) were subjected to mutational analysis. Five mutants mimicking deamidation (N→D, Q→E) at the substrate binding cleft showed moderate to no effect on thermostability and k cat and K M for maltoheptaose and amylose. Notably, the mutations improved laundry wash efficiency in detergents at pH 8.5 and 10.0. Replacing three reducing sugar reactive side chains (K→M, R→L) at a distant substrate binding region and two SBSs enhanced wash performance especially in liquid detergent at pH 8.5, slightly improved enzymatic activity and maintained thermostability. Wash performance was most improved (5-fold) for the N265D mutant near substrate binding subsite +3.


Water Policy ◽  
2016 ◽  
Vol 19 (1) ◽  
pp. 181-195 ◽  
Author(s):  
Huiqing Han ◽  
Yuxiang Dong

Water supply is an important freshwater ecosystem service provided by ecosystems. Water shortages resulting from spatio-temporal heterogeneity of climate condition or human activities present serious problems in the Guizhou Province of southwest China. This study aimed to analyze the spatio-temporal changes of water supply service using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model, explore how climate and land-use changes impact water supply provision, and discuss the impact of parameters associated with climate and land-use in the InVEST model on water supply in the region. We used data and the model to forecast trends for the year 2030 and found that water supply has been declining in the region at the watershed scale since 1990. Climate and land-use change played important roles in affecting the water supply. Water supply was overwhelmingly driven by the reference evapotranspiration and annual average precipitation, while the plant evapotranspiration coefficients for each land-use type had a relatively small effect. The method for sensitivity analysis developed in this study allowed exploration of the relative importance of parameters in the InVEST water yield model. The Grain-for-Green project, afforestation, and urban expansion control should be accelerated in this region to protect the water supply.


2019 ◽  
pp. 227-232
Author(s):  
Edward B. Barbier

This concluding chapter looks at the future of water. There are two possible paths for managing water. First, if the world continues with inadequate governance and institutions, incorrect market signals, and insufficient innovations to improve efficiency and manage competing demands, most chronic water and scarcity problems will continue to worsen. The world will see a future of declining water security, freshwater ecosystem degradation, and increasing disputes and conflicts over remaining water resources. The alternative path to managing water is the one offered by this book. If, in anticipation of the coming decades of increasing water scarcity, humankind is able to develop appropriate governance and institutions for water management, instigate market and policy reforms, and address global management issues, then improved innovation and investments in new water technologies and better protection of freshwater ecosystems should secure sufficient beneficial water use for a growing world population.


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