In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

2017 ◽  
Vol 34 ◽  
pp. 291-306 ◽  
Author(s):  
G Russmueller ◽  
◽  
L Winkler ◽  
R Lieber ◽  
R Seemann ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Bone Substitute Materials ◽  
In Vitro Effects ◽  
Substitute Materials ◽  
Human Osteoclasts

scholarly journals Influence of Absorbable Calcium Sulfate-Based Bone Substitute Materials on Human Haemostasis—In Vitro Biological Behavior of Antibiotic Loaded Implants

Materials ◽  
2018 ◽  
Vol 11 (6) ◽  
pp. 935 ◽  
Author(s):  
Dominik Pförringer ◽  
Norbert Harrasser ◽  
Marc Beirer ◽  
Moritz Crönlein ◽  
Axel Stemberger ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Calcium Sulfate ◽  
Biological Behavior ◽  
Bone Substitute Materials ◽  
Substitute Materials

scholarly journals Antimicrobial Formulations of Absorbable Bone Substitute Materials as Drug Carriers Based on Calcium Sulfate

2016 ◽  
Vol 60 (7) ◽  
pp. 3897-3905 ◽  
Author(s):  
D. Pförringer ◽  
A. Obermeier ◽  
M. Kiokekli ◽  
H. Büchner ◽  
S. Vogt ◽  
...  
Keyword(s):  
Bone Substitute ◽  
Ex Vivo ◽  
Release Kinetics ◽  
Drug Carriers ◽  
Prolonged Release ◽  
Clinical Parameters ◽  
Bone Substitute Materials ◽  
Substitute Materials

ABSTRACTSubstitution of bones is a well-established, necessary procedure to treat bone defects in trauma and orthopedic surgeries. For prevention or treatment of perioperative infection, the implantation of resorbable bone substitute materials carrying antibiotics is a necessary treatment. In this study, we investigated the newly formulated calcium-based resorbable bone substitute materials containing either gentamicin (CaSO4-G [Herafill-G]), vancomycin (CaSO4-V), or tobramycin (Osteoset). We characterized the released antibiotic concentration per unit. Bone substitute materials were implanted in bones of rabbits via a standardized surgical procedure. Clinical parameters and levels of the antibiotic-releasing materials in serum were determined. Local concentrations of antibiotics were measured using antimicrobial tests of bone tissue. Aminoglycoside release kineticsin vitroper square millimeter of bead surface showed the most prolonged release for gentamicin, followed by vancomycin and, with the fastest release, tobramycin.In vivolevel in serum detected over 28 days was highest for gentamicin at 0.42 μg/ml, followed by vancomycin at 0.11 μg/ml and tobramycin at 0.04 μg/ml. The clinical parameters indicated high biocompatibility for materials used. None of the rabbits subjected to the procedure showed any adverse reaction. The highest availability of antibiotics at 14.8 μg/g on day 1 in the cortical tibiaex vivowas demonstrated for gentamicin, decreasing within 14 days. In the medulla, vancomycin showed a high level at 444 μg/g on day 1, decreasing continuously over 14 days, whereas gentamicin decreased faster within the initial 3 days. The compared antibiotic formulations varied significantly in release kinetics in serum as well as locally in medulla and cortex.

The study on calcium polyphosphate/poly-amino acid composite for supportive bone substitute materials in vitro

2021 ◽  
Author(s):  
Jinbo Hu ◽  
Rongguang Zhang ◽  
Hong Chen ◽  
Yanan Wu ◽  
Lichao Chen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mechanical Strength ◽  
Human Body ◽  
Bone Substitute ◽  
Calcium Polyphosphate ◽  
Proliferation And Differentiation ◽  
Bone Substitute Materials ◽  
Substitute Materials ◽  
The Ideal

In addition to the sufficient mechanical strength to burden the force of the human body, the ideal supporting implant material should have bioactivity to stimulate the cells proliferation and differentiation...

scholarly journals Does Platelet-Rich Fibrin Enhance the Early Angiogenetic Potential of Different Bone Substitute Materials? An In Vitro and In Vivo Analysis

Biomedicines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 61
Author(s):  
Sebastian Blatt ◽  
Daniel G.E. Thiem ◽  
Andreas Pabst ◽  
Bilal Al-Nawas ◽  
Peer W. Kämmerer
Keyword(s):  
Growth Factor ◽  
Bone Substitute ◽  
Tissue Growth ◽  
Platelet Rich Fibrin ◽  
Vessel Formation ◽  
Branching Points ◽  
Bone Substitute Materials ◽  
Substitute Materials

The impaired angiogenic potential of bone substitute materials (BSMs) may limit regenerative processes. Therefore, changes in the angiogenetic properties of different BSMs in combination with platelet-rich fibrin (PRF) in comparison to PRF alone, as well as to native BSMs, were analyzed in vitro and in vivo to evaluate possible clinical application. In vitro, four BSMs of different origins (allogeneic, alloplastic, and xenogeneic) were biofunctionalized with PRF and compared to PRF in terms of platelet interaction and growth factor release (vascular endothelial growth factor (VEGF), tissue growth factor ß (TGFß) and platelet-derived growth factor (PDGF)) after 15 min. To visualize initial cell–cell interactions, SEM was performed. In vivo, all BSMs (±PRF) were analyzed after 24 h for new-formed vessels using a chorioallantoic membrane (CAM) assay. Especially for alloplastic BSMs, the addition of PRF led to a significant consumption of platelets (p = 0.05). PDGF expression significantly decreased in comparison to PRF alone (all BSMs: p < 0.013). SEM showed the close spatial relation of each BSM and PRF. In vivo, PRF had a significant positive pro-angiogenic influence in combination with alloplastic (p = 0.007) and xenogeneic materials (p = 0.015) in comparison to the native BSMs. For bio-activated xenogeneic BSMs, the branching points were also significantly increased (p = 0.005). Finally, vessel formation was increased for BSMs and PRF in comparison to the native control (allogeneic: p = 0.046; alloplastic: p = 0.046; and xenogeneic: p = 0.050). An early enhancement of angiogenetic properties was demonstrated when combining BSMs with PRF in vitro and led to upregulated vessel formation in vivo. Thus, the use of BSMs in combination with PRF may trigger bony regeneration in clinical approaches.

scholarly journals Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

2021 ◽  
Vol 9 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Eik Schiegnitz ◽  
Diana Heimes ◽  
Henning Staedt ◽  
...  
Keyword(s):  
Metabolic Activity ◽  
Bone Substitute ◽  
Bone Morphogenetic Protein 2 ◽  
Bovine Bone ◽  
Human Osteoblasts ◽  
Time Points ◽  
Significant Difference ◽  
Bone Substitute Materials ◽  
Substitute Materials

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-treatment with i-PRF increased viability in all groups with the highest values for CB-i-PRF after 3 and 7 and for CX-i-PRF after 10 days. For metabolic activity, the highest rate among non-i-PRF groups was seen for MO at day 3 and for CB at day 7 and 10. Here, i-PRF groups showed higher values than non-i-PRF groups (highest values: CB + i-PRF) at all time points. There was no difference in ALP-expression between groups. For OCN expression in non-i-PRF groups, CB showed the highest values after day 3, CX after day 7 and 10. Among i-PRF-groups, the highest values were seen for CX + i-PRF. At day 3, the highest BMP-2 expression was observed for CX. Here, for i-PRF groups, the highest increase was seen for CX + i-PRF at day 3. At day 7 and 10, there was no significant difference among groups.ConclusionXBSM sintered under high temperature showed increased HOB viability and metabolic activity through the whole period when compared to XBSM manufactured at lower temperatures. Overall, the combination of XBSM with i-PRF improved all cellular parameters, ALP and BMP-2 expression at earlier stages as well as OCN expression at later stages.

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