Synergistic Inhibitory Activity of Bacillomycin D, Surfactin and Nisin against Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, Responsible for Cardboard Spoilage

Corrugated cardboard boxes are one of the largest paper-based packaging forms used for shipping and handling of wide variety of products in different end-use industries due to low cost, low weight and recyclability. Due to its organic composition, they are highly susceptible to spoilage from heat-resistant microbial spores, leading to economic losses and health risks. In this study, the efficacy of lipopeptides produced from Bacillus amyloliquefaciens MTCC 10456 against thermotolerant Thermoascus crustaceus, Neosartorya hiratsukae and Bacillus subtilis, isolated from spoiled cardboard boxes, was investigated. Lipopeptides were isolated by salt-precipitation of fermentation broth and activity-guided Reverse Phase-High Performance Liquid Chromatography (RP-HPLC). Inhibitory fractions consisted of bacillomycin D and surfactin, which were identified using liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS/MS) analysis. Mixture of lipopeptides with nisin (3:2 w/w) asserted significant synergistic effect on the tested pathogens which reduced the minimum inhibitory concentrations (MIC) values and increased their inhibition spectra. Preservative coating containing lipopeptides and nisin was applied on the corrugated cardboard surfaces by mixing with starch-based additive by spread-coating method. It demonstrated biopreservative efficacy against the targeted microorganisms at during the observational period of 180 days. Reduction in microbial count of 4 log cycles was observed in 20 days and showed controlled release of coated peptides which indicate its suitability for packaging purposes. Findings from this study suggests an effective and scalable strategy to prevent microbial spoilage thereby extending the storage period of cardboard boxes.

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Analysis of Hydrolyzable Tannins and Other Phenolic Compounds in Emblic Leafflower (Phyllanthus emblica L.) Fruits by High Performance Liquid Chromatography–Electrospray Ionization Mass Spectrometry

Journal of Agricultural and Food Chemistry ◽

10.1021/jf302925v ◽

2012 ◽

Vol 60 (35) ◽

pp. 8672-8683 ◽

Cited By ~ 52

Author(s):

Baoru Yang ◽

Maaria Kortesniemi ◽

Pengzhan Liu ◽

Maarit Karonen ◽

Juha-Pekka Salminen

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

High Performance ◽

Ionization Mass ◽

Phyllanthus Emblica ◽

Hydrolyzable Tannins

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Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

Planta Medica ◽

10.1055/a-1240-6186 ◽

2020 ◽

Author(s):

Omer I. Fantoukh ◽

Yan-Hong Wang ◽

Abidah Parveen ◽

Mohammed F. Hawwal ◽

Gadah A. Al-Hamoud ◽

...

Keyword(s):

Liquid Chromatography ◽

Dietary Supplements ◽

High Performance ◽

Moringa Oleifera ◽

Negative Ion ◽

Array Detector ◽

Ionization Mass ◽

Fractionation Process ◽

Chromatography Method ◽

Drumstick Tree

Abstract Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1 – 9 were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5) and quercetin 3-O-(6-O-malonyl)-β−D-glucopyranoside (6) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6. Niazirin (2) was detected in amounts between 0.010 – 0.049 mg/100 mg while compound 1 was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

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