Assessment of molecular diversity among mungbean [Vigna radiata (L.) Wilczek] genotypes using EST-SSR marker

The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50 % flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP-11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.

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Genetic diversity of the mungbean (Vigna radiata, Leguminosae) genepool on the basis of microsatellite analysis

Australian Journal of Botany ◽

10.1071/bt07105 ◽

2007 ◽

Vol 55 (8) ◽

pp. 837 ◽

Cited By ~ 48

Author(s):

Chontira Sangiri ◽

Akito Kaga ◽

Norihiko Tomooka ◽

Duncan Vaughan ◽

Peerasak Srinives

Keyword(s):

Genetic Diversity ◽

South Asia ◽

Vigna Radiata ◽

Ssr Marker ◽

Allelic Diversity ◽

Microsatellite Analysis ◽

Centre Of Diversity ◽

Ssr Primers ◽

Germplasm Diversity ◽

Large Representative

A large representative collection of mungbean (Vigna radiata (L.) Wilczek) consisting of 415 cultivated, 189 wild and 11 intermediate accessions was analysed by using 19 SSR primers. These SSR primers showed polymorphism in wild and cultivated mungbean and were selected from those available for the related species azuki bean [V. angularis (Willd.) Ohwi & Ohwi]. One or more SSR primer for each linkage group (on the basis of the azuki linkage map) was analysed. In total, 309 alleles were detected and of these, about twice as many were detected in wild (257 alleles) as in cultivated accessions (138 alleles). The results show that Australia and New Guinea represent a distinct centre of diversity for wild mungbean. Cultivated mungbean has greatest diversity in South Asia, which supports the view that South Asia is where this crop was domesticated. SSR marker allelic diversity for cultivated mungbean has distinct regional variation, with high variation in South and West Asia. The present study represents the first comprehensive analysis of wild and cultivated mungbean germplasm diversity by SSR markers, and highlights specific genetic diversity that might be used to broaden the genetic base of currently grown mungbean cultivars.

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Assessment of molecular diversity of greengram [Vigna radiata (L.) Wilzek] through RAPD

Legume Research - An International Journal ◽

10.18805/lr.v0iof.9442 ◽

2016 ◽

Author(s):

Gunnjeet Kaur ◽

Arunabh Joshi ◽

Devendra Jain ◽

Ganesh Rajamani ◽

Divya Vyas

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Polymorphism Information Content ◽

Vigna Radiata ◽

Molecular Diversity ◽

Rapd Analysis ◽

Similarity Coefficient ◽

Green Gram ◽

Randomly Amplified Polymorphic Dna ◽

Legume Crop

A total twenty three genotypes of green gram (Vigna radiata) were subjected to Randomly amplified polymorphic DNA (RAPD) analysis for molecular characterization. A total of 25 randomly selected decamers were screened, out of which only 15 generated 126 amplification products from which 117 bands were found polymorphic, the average polymorphism being 93.48%. The total number of amplified bands varied between 2 (primer OPP-09) to 17 (primer OPA-1) with an average of 9.5 bands per primer. The overall size of PCR amplified products ranged between 200 bp to 2900 bp. The average Polymorphism Information Content(PIC) was 0.32 ranging from 0.17 to 0.46. Primer OPA-01 and OPP-06 detected two unique bands ranged between 250 bp to 2500 bp in two genotypes (PUSA-672 and HUM-12). Jaccard's similarity coefficient values ranged from 0.28-0.90 with an average of 0.59. Based on dendrogram generated through UPGMA method and PCA, most of the genotypes got divided into four main clusters. Genotype EC-398885 lay far apart and thus showed maximum genetic distance. The assessment of genetic diversity is a prerequisite and important step for the improvement of any legume crop. Thus, present results of the present study could be further extrapolated to other green gram accessions in Vigna germplasm.

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Lectins from Vigna radiata - A potential health supplement

Planta Medica ◽

10.1055/s-0031-1282615 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

SR Singh ◽

PA Tatke ◽

VP Naharwar

Keyword(s):

Vigna Radiata ◽

Potential Health

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IMPACT OF FERTILIZERS APPLICATION ON SEED GERMINATION AND SEEDLING GROWTH OF VIGNA RADIATA

FLORA AND FAUNA ◽

10.33451/florafauna.v24i2pp323-329 ◽

2018 ◽

Vol 24 (2) ◽

Author(s):

SUPRIYA DIXIT ◽

R. K. GUPTA

Keyword(s):

Seed Germination ◽

Leaf Area ◽

Seedling Growth ◽

Vigna Radiata ◽

Agricultural Research ◽

Dry Weight ◽

Fertilizer Application ◽

Research Field ◽

Chemical Fertilizer ◽

Seedling Length

Currently, a real challenge for the workers in the agricultural research field is to stop or reduce the use of expensive agrochemicals/ chemical fertilizers which are hazardous to the environment as well as human health. Present study was aimed to improve the growth and obtain optimum yield of Vigna crop with eco-friendly, non-toxic way and to reduce the use of agrochemical/chemical fertilizer application in agricultural activities. A pot experiment was conducted to study the effect of chemical fertilizer (DAP) and biofertilizer ( Rhizobium strain) separately and in combination on seed germination and seedling growth (at 30 days) based on morphological parameters such as seedling length (cm), fresh weight (g), dry weight (g) and leaf area (cm)2 of Vigna radiata (L.) Wilczek. After one month (30 Days) observations, it was found that seedling length, fresh and dry weights and leaf area were maximum in T4 and minimum in T15, T7 and T8 favored improved seedling length and leaf area whereas T7, T8, and T9 favored improved fresh and dry weights as compared to control.

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