scholarly journals Pengaruh jumlah subkultur dan media sub-optimal terhadap pertumbuhan dan kemampuan regenerasi kalus tebu (Saccharum officinarum L.) (Effect of repeated subculture and suboptimum media on the growth of sugarcane calli (Saccharum officinarum L.))

2016 ◽  
Vol 84 (1) ◽  
Author(s):  
Hayati MINARSIH ◽  
. Suharyo ◽  
Imron RIYADI ◽  
Diah RATNADEWI
Keyword(s):  
Saccharum Officinarum ◽  
Coconut Water ◽  
Basic Medium ◽  
Callus Initiation ◽  
Young Leaves ◽  
Three Stages ◽  
Sugarcane Productivity ◽  
Repeated Subculture ◽  
Better Than

Sugarcane (Saccharum officinarum L.) is an important crop for sugar production. One attempt to increase sugarcane productivity is through micropropagation and quality improvement of sugarcane seedlings in vitro. This research aimed to study the effect of repeated subcultures on callus capacity for regeneration and plant survival in acclimatization phase, as well as the influence of suboptimum media on the recovery capability of sugarcane callus to proliferate in vitro. Fourth subcultured sugarcane callus derived from young leaves were used as material in this research. Basic medium of Murashige and Skoog (MS) added with 3 mg/L 2,4-D, 10% coconut water, and 3% sucrose was used for callus initiation. For callus regeneration, the MS medium was supplemented with 2 mg/L BAP, 0.2 mg/L IAA, 10% coconut water, and 3% sucrose. Study on the effect of subculture numbers consisted of three stages, i.e. initiation, regeneration, and acclimatization, while the study on resting phase or the use of sub-optimal media included six treatment media and two pathways. Results showed that the fifth subcultures produced embryoid callus (91%), the highest non mucilaginous callus (97%), and the highest abnormality rate (6%). Results from the suboptimum media treatment, showed that B pathway (4 week resting phase) was better than the A pathway (8 week resting phase), based on fresh weight and callus abnormality percentage. A and B pathways indicated that the growth of callus can be recovered when it was grown back to the normal media and 1.5D-MS treatment of the resting phase showed the best growth and appearance. 

scholarly journals Pengaruh jumlah subkultur dan media sub-optimal terhadap pertumbuhan dan kemampuan regenerasi kalus tebu (Saccharum officinarum L.) (Effect of Repeated Subculture and suboptimum media on the Growth of Sugarcane Calli (Saccharum officinarum L.))

2016 ◽  
Vol 84 (1) ◽  
Author(s):  
Hayati MINARSIH ◽  
. Suharyo ◽  
Imron RIYADI ◽  
Diah RATNADEWI
Keyword(s):  
Saccharum Officinarum ◽  
Coconut Water ◽  
Basic Medium ◽  
Callus Initiation ◽  
Young Leaves ◽  
Three Stages ◽  
Sugarcane Productivity ◽  
Repeated Subculture ◽  
Better Than

Sugarcane (Saccharum officinarum L.) is an important crop for sugar production. One attempt to increase sugarcane productivity is through micropropagation and quality improvement of sugarcane seedlings in vitro. This research aimed to study the effect of repeated subcultures on callus capacity for regeneration and plant survival in acclimatization phase, as well as the influence of suboptimum media on the recovery capability of sugarcane callus to proliferate in vitro. Fourth subcultured sugarcane callus derived from young leaves were used as material in this research. Basic medium of Murashige and Skoog (MS) added with 3 mg/L 2,4-D, 10% coconut water, and 3% sucrose was used for callus initiation. For callus regeneration, the MS medium was supplemented with 2 mg/L BAP, 0.2 mg/L IAA, 10% coconut water, and 3% sucrose. Study on the effect of subculture numbers consisted of three stages, i.e. initiation, regeneration, and acclimatization, while the study on resting phase or the use of sub-optimal media included six treatment media and two pathways. Results showed that the fifth subcultures produced embryoid callus (91%), the highest non mucilaginous callus (97%), and the highest abnormality rate (6%). Results from the suboptimum media treatment, showed that B pathway (4 week resting phase) was better than the A pathway (8 week resting phase), based on fresh weight and callus abnormality percentage. A and B pathways indicated that the growth of callus can be recovered when it was grown back to the normal media and 1.5D-MS treatment of the resting phase showed the best growth and appearance. 

scholarly journals Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

2019 ◽  
Vol 17 (1) ◽  
pp. 33-37
Author(s):  
A. Z. Revutskaya ◽  
A. V. Holubenko ◽  
N. V. Nuzhyna ◽  
H. O. Rudik ◽  
N. Yu. Taran
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Nutrient Medium ◽  
Callus Growth ◽  
Basic Medium ◽  
High Concentration ◽  
Salvia Hispanica ◽  
Callus Initiation ◽  
Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

scholarly journals Comparative Analysis of Solasodine from in vitro and in vivo Cultures of Solanum nigrum Linn.

1970 ◽  
Vol 5 (1) ◽  
pp. 99-103 ◽  
Author(s):  
N Yogananth ◽  
R Bhakyaraj ◽  
A Chanthuru ◽  
S Parvathi ◽  
S Palanivel
Keyword(s):  
Callus Induction ◽  
Solanum Nigrum ◽  
Plant Part ◽  
Ms Medium ◽  
Engineering And Technology ◽  
Callus Initiation ◽  
Young Leaves ◽  
Grown Plant

An efficient protocol was devised for rapid callus induction of Solanum nigrum Linn. from young leaves. MS medium supplemented with different concentrations IAA (1-3 mg/l) with BAP (0.5 mg/l) and NAA (1-3 mg/l) with BAP (0.5 mg/l) for callus initiation. The growth of the calli derived from leaves increased with time of incubation and remained almost constant after 30 days. For solasodine estimation, the field grown plant part of young leaves and in vitro callus (0.5 g each) were weighed and extracted thrice with methanol and subjected to HPLC. The solasodine content of field grown leaves extracts was 0.0798 mg g-1 whereas the solasodine content in the in vitro callus extracts were 0.142 mg g-1 in 2.5 mgL-1 IAA + 0.5 mgL-1 BAP, followed by 0.1162 mg g-1 in 2 mgL-1 NAA + 0.5 mgL-1 BAP. Key words: Callus induction; Solasodine; Solanum nigrum; medicinal plant DOI: 10.3126/kuset.v5i1.2850 Kathmandu University Journal of Science, Engineering and Technology Vol.5, No.1, January 2009, pp 99-103

scholarly journals Propagación vegetativa in vitro a partir de hojas jóvenes de caña de azúcar (Saccharum officinarum L.)

2016 ◽  
Vol 8 (1) ◽  
pp. 74
Author(s):  
José M. Cuellar
Keyword(s):  
El Salvador ◽  
Acid Concentration ◽  
Growth And Development ◽  
Saccharum Officinarum ◽  
The Other ◽  
In Vitro Cultivation ◽  
Young Leaves ◽  
Macro Nutrients

This paper reports on the first in vitro cultivation tests done in El Salvador. The responses of cultivars MEX 70-485 and MEX 68-P-23 on various propagation stages, were evaluated in vitro. Young leaves still in the rolled stage were utilized. Explants 5 mm in lenght were placed on a Murashige and Skoog (1962) medium, modified for each stage of planttet recovery as follows: For the development and growth of “calluses” dosis, 2.0, 2.5 and 3.0 mg/l of 2.4/D were added and for differentiation, it was subtracted. To induce rhyzogenesis we used a medium with half of the macro-nutrients , one with the complete salts the other with the complete salts plus a supplement of 5 mg/l of AIA. Outstanding results were the following: In the phase of “calluses” formation, the major problem was the phenolization of the explants; the best 2.4-D acid concentration was 3.0 mgl, for both the calluses formation as for their growth and development. The differentiation was obtained with the renoval of 2.4-D acid from the medium, and the rhyzogenesis was favored with the supplement of 5 mg/l of AIA

Selecting for nutritive value in Digitaria milanjiana. 2. Intake and digestibility of divergently selected full-sibs compared with Digitaria decumbens

1986 ◽  
Vol 26 (5) ◽  
pp. 551 ◽  
Author(s):  
DJ Minson ◽  
JB Hacker
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
In Vitro Digestibility ◽  
Digitaria Decumbens ◽  
Young Leaves ◽  
Significant Comparison ◽  
Metabolisable Energy ◽  
Better Than

We compare the voluntary intake and digestibility of 6 genotypes selected from 3 F1 families of Digitaria milanjiana for high or low leaf digestibility, and D. decumbens (pangola grass). Swards were harvested on 6 occasions (4 by 4-week regrowths, 1 by 10-week and 1 by 14-week regrowth) and fed to sheep in metabolism cages. Averaged over families and regrowths, high digestibility selections were significantly superior to low digestibility selections in digestibility, voluntary dry matter intake and intake of metabolisable energy. There was a large range in leafiness of the genotypes. Correlations between leafiness and in vivo digestibility, dry matter (DM) intake and intake of metabolisable energy were positive and statistically significant. Comparison of full-sibs at the same percentage stem (80 and 100%) suggested a higher DM intake, digestibility and intake of metabolisable energy of stem from genotypes selected for high leaf digestibility. Averaged over harvests, none of the selected genotypes was significantly superior to pangola grass in digestibility but all were superior in DM intake and 5 in intake of metabolisable energy. The best selected genotype was 35% better than pangola grass in terms of intake of metabolisable energy. Differences in in vitro digestibility of young leaves from vegetative tillers of full sibs, the characteristic on which the genotypes had been selected, were maintained under sward conditions, although differences were reduced.

scholarly journals PERBANYAKAN NILAM (Pogostemon cablin Benth) MENGGUNAKAN MEDIA DASAR ALTERNATIF SECARA IN VITRO In vitro multiplication of Patchouli uses alternative primary medium

Perspektif ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 139
Author(s):  
Amalia Amalia
Keyword(s):  
Tissue Culture ◽  
Complex Compounds ◽  
Coconut Water ◽  
Basic Medium ◽  
Culture Methods ◽  
Culture Techniques ◽  
Murashige Skoog ◽  
Long Time ◽  
Tissue Culture Propagation

<p align="center">Benih sangat menentukan dalam keberhasilan usahatani nilam. Perbanyakan benih secara konvensional vegetatif sangat mudah menularkan penyakit dan membutuhkan waktu yang relatif lama, seperti benih nilam yang diperbanyak selama ini dengan setek. Cara ini memiliki kendala, yang diharapkan dapat diatasi dengan teknik kultur jaringan. Media merupakan faktor utama dalam perbanyakan kultur jaringan. Perbanyakan dan perkembangbiakan tanaman nilam dengan metode kultur jaringan secara umum sudah dapat dilakukan tetapi untuk keberhasilannya sangat tergantung pada jenis media, terutama bila ditinjau dari sisi ekonomi. Karena aplikasi teknologi kultur jaringan untuk tanaman nilam masih dirasakan mahal. Tulisan ini mengulas tentang penggunaan media untuk menekan biaya media kultur jaringan yaitu dengan menggantikan media dasar MS (Murashige-Skoog), ZPT dan vitamin dengan media dasar alternatif dan air kelapa 10%. Air kelapa merupakan salah satu diantara beberapa persenyawaan kompleks alamiah yang sering digunakan dalam kultur jaringan. Sedangkan media dasar alternatif berupa pupuk daun dapat berfungsi sebagai penyedia unsur hara makro mikro  dengan komposisi  N:P:K (20:20:20). Hal ini untuk mengatasi permasalahan, agar media kultur jaringan menjadi relatif murah, dan harga jual benih lebih terjangkau. Air kelapa yang digunakan berasal dari kelapa hijau yang dicirikan dengan volume air masih memenuhi buah dan keadaan endosperm (daging kelapa) yang belum menebal.  Tetapi meski bahan alternatif ini sudah banyak digunakan untuk media pengganti kultur jaringan karena relatif mudah tersedia, murah, menghasilkan benih seragam dan sehat, ternyata belum dapat menunjukkan hasil yang setara dibandingkan dengan penggunaan media MS dalam perbanyakan tunas nilam secara kultur jaringan. Oleh karena itu berbagai penelitian  perbanyakan tanaman nilam dengan berbagai metode kultur jaringan agar menghasilkan benih yang murah, sehat, seragam dan dalam jumlah besar masih perlu terus diupayakan.</p><p> </p><p align="center">ABSTRACT</p><p> The quality of seeds are very  important in patchoulli cultivation. Cutting multiplication seeds are usually easy in transmitting diseases and relatively need a long time to grow. So far patchouli seeds  obtained conventionally with cutting has some constraints, hence tissue culture techniques becomes the solution once. The success of propagation and breeding of plants with tissue culture methods in patchouly is already conducted but is still expensive to be implemented. The paper review patchouli tissue culture propagation  by replacing basic media MS (murashige-skoog, Growth Regulating Substances (GRS) and vitamine with alternate  basic medium and 10% coconut water Coconut water is one of several natural complex compounds that are often used in tissue culture. The alternative medium as leaf fertilizer can serve as <span style="text-decoration: line-through;">a</span> micro and macro nutrient provider with composition N: P: K (20:20:20). Hopefully, It could become the solution to make tissue culture of patchoulli seeds cheaper and more available. Actually, eventhough the overall substitution of MS medium with full alternative media has already used in limited areas, it has not able yet  showing equal results with the use of basic medium MS media in tissue culture patchouli multiplication. Therefore, the researches on patchouli tissue culture should be continued to achieve the huge number, healthy, unity, and unexpensive seeds.</p><p> </p>

scholarly journals The effect of coconut water and benzyl amino purine (BAP) addition to the growth of pineapple from Sipahutar North Sumatera in vitro condition

2018 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
Harifah Insani ◽  
Fauziyah Harahap ◽  
Diky Setya Diningrat
Keyword(s):  
Tissue Culture ◽  
Leaf Length ◽  
Coconut Water ◽  
Basic Medium ◽  
Shoot Height ◽  
Culture Techniques ◽  
Base Medium ◽  
Randomized Design ◽  
Benzyl Amino Purine

Background: Pineapple fruit used in this study was obtained from Sipahutar sub-district, North Tapanuli regency, North Sumatra which was a superior commodity fruit. Propagation of the Sipahutar pineapple plant was conducted vegetatively using bud stems and crown shoots, but the amount was limited to be planted in large areas. Therefore, propagation through tissue culture techniques could be used as an alternative solution.Objective: Explants source were taken from plantlets in vitro aged 3 months were grown on Murashige and Skoog (MS) basic medium with the addition of Indole Acetic Acid (IAA) 1 ppm.Methods: Sipahutar pineapple tissue culture work has been done in YAHDI Plant Tissue Culture Laboratory, Medan. The study design used a factorial completely randomized design. The first factor is coconut water with concentration of 0%, 10%, 20%, and 30%, second factor is Benzyl Amino Purine (BAP) with concentration 0 ppm, 2 ppm and 4 ppm, so there were 12 combination with 3 repetition.Result: The results showed that the addition of coconut water on MS base medium with IAA of 1 ppm gave a significant effect on the number of leaf yield and shoot height. The provision of BAP on basic MS medium with IAA of 1 ppm gave a significant effect on the number of shoots produced, the number of leaves produced, the length of the leaves, the width ofleaves and the length of shoots. The interaction of coconut water and BAP addition on MS base medium with IAA of 1 ppm gave significant effect on leaf length, leaf width and shoot length as well.Conclusions: MS + IAA basic media combined with coconut water + BAP could stimulate the growth of Sipahutar pineapple in vitro.  

scholarly journals Regeneration of bulblets on bulb scale segments of hyacinth in vitro.

1973 ◽  
Vol 21 (2) ◽  
pp. 129-138 ◽  
Author(s):  
R.L.M. Pierik ◽  
M.A. Ruibing
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Continuous Light ◽  
Basic Medium ◽  
Light Bulb ◽  
Holding Period ◽  
Regenerative Ability ◽  
Effects Of Temperature ◽  
Better Than

Hyacinth bulbs, cv. Pink Pearl, harvested in June were held at 25 deg C for 1-5 months, after which bulb scales were removed for tissue culture in modified basic medium. The length of the holding period had little effect on the number of bulblets that regenerated, but the average bulb weight increased as the holding period lengthened, attaining a maximum with bulbs held until 1-15 October. There were wide differences in regenerative ability and bulblet growth between bulbs of the same harvest and size. The number of bulblets that regenerated was not affected by scale age, but bulblet growth from the older scales (nos. 7-18) was much better than from the younger scales. Regeneration and growth of explants from the proximal parts of the scales was much better than from the distal parts, and 3-cm-long explants gave much better results than shorter ones. Inverting the explants in the culture medium was favourable to regeneration and growth. In further studies on the effects of temperature and light, bulb regeneration was accelerated by raising the temperature, the optimum being 21.6 deg . The greatest number of bulblets/explant, however, was obtained at 13 deg , and the highest bulb weight at 21.6 or 24.8 deg . There were no differences in regeneration and bulblet growth between scales grown in continuous light or in darkness. (Abstract retrieved from CAB Abstracts by CABI’s permission)

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

Motilitas dan Vabilitas Spermatozoa Ayam Kampung pada Suhu 5°C Menggunakan Pengencer dan Lama Simpan yang Berbeda

2018 ◽  
Vol 5 (2) ◽  
pp. 102
Author(s):  
Enike Dwi Kusumawati ◽  
Selvinus Lawu Woli ◽  
Aju Tjatur Nugroho Krisnaningsih ◽  
Waluyo Edi Susanto ◽  
Syam Rahadi
Keyword(s):  
Data Analysis ◽  
Storage Temperature ◽  
Coconut Water ◽  
Laboratory Research ◽  
Ringer Lactate ◽  
Randomized Design ◽  
Native Chicken ◽  
Lactate Solution ◽  
Better Than

ABSTRAKPenelitian ini dilakukan untuk mengetahui motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5oC menggunakan pengencer dan lama simpan yang berbeda. Metode yang digunakan dalam penelitian ini adalah penelitian laboratorium menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan pengencer ringer lactat solution, air kelapa dan tanpa pengencer serta lama simpan 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, masing-masing diulang 10 kali. Variabel yang diamati yaitu motilitas dan viabilitas spermatozoa. Analisa data yang digunakan adalah analisis varian. Hasil analisis data menunjukkan bahwa motilitas dan viabilitas spermatozoa menggunakan pengencer ringer lactat solution lebih tinggi (P<0,01) serta dapat bertahan sampai lama simpan 24 jam dibandingkan air kelapa dan tanpa pengencer. Adapun nilai motilitas ringer lactat solution, air kelapa dan tanpa pengencer pada lama simpan 24 jam masing-masing sebesar 43,5±17,17%; 8±4,83%; 6,5±2,4%, sedangkan nilai viabilitasnya sebesar 83,2±7,25%; 64,6±3,20%; dan 63,1±2,33%. Kesimpulan dari hasil penelitian ini adalah ringer lactat solution lebih baik dibandingkan air kelapa dan tanpa pengencer dalam mempertahankan kualitas semen ayam kampung pada suhu simpan 5oC sampai lama simpan 24 jam.Kata Kunci : air kelapa, ayam kampung, motilitas, spermatozoa, viabilitas  ABSTRACTThis study was conducted to determine the motility and viability of spermatozoa of Native chickens at 5oC using different diluents and time storage. The method used in this study was laboratory research using Factorial Completely Randomized Design with ringer lactate solution, coconut water and without diluent at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 hours of time storage each repeated 10 times. The variables observed were motility and viability of sperm. Data analysis used is variance analysis. The results of data analysis showed that the motility and viability of spermatozoa using ringer lactate solution diluents was higher (P <0.05) than coconut water and without diluents. The motility values of ringer lactat solution, coconut water and without diluents were 43,5±17,17%; 8±4,83%; 6,5±2,4% respectively, while the viability values were 83,2±7,25%; 64,6±3,20% and 63,1±2,33%. The conclusion of this study is that ringer lactat solution is better than coconut water an without diluents in maintaining the quality of Native chicken semen at a storage temperature of 5oC until 24 hours.Keywords: coconut water, motility, native chicken, sperm, viability

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