Selecting for nutritive value in Digitaria milanjiana. 2. Intake and digestibility of divergently selected full-sibs compared with Digitaria decumbens

1986 ◽  
Vol 26 (5) ◽  
pp. 551 ◽  
Author(s):  
DJ Minson ◽  
JB Hacker

We compare the voluntary intake and digestibility of 6 genotypes selected from 3 F1 families of Digitaria milanjiana for high or low leaf digestibility, and D. decumbens (pangola grass). Swards were harvested on 6 occasions (4 by 4-week regrowths, 1 by 10-week and 1 by 14-week regrowth) and fed to sheep in metabolism cages. Averaged over families and regrowths, high digestibility selections were significantly superior to low digestibility selections in digestibility, voluntary dry matter intake and intake of metabolisable energy. There was a large range in leafiness of the genotypes. Correlations between leafiness and in vivo digestibility, dry matter (DM) intake and intake of metabolisable energy were positive and statistically significant. Comparison of full-sibs at the same percentage stem (80 and 100%) suggested a higher DM intake, digestibility and intake of metabolisable energy of stem from genotypes selected for high leaf digestibility. Averaged over harvests, none of the selected genotypes was significantly superior to pangola grass in digestibility but all were superior in DM intake and 5 in intake of metabolisable energy. The best selected genotype was 35% better than pangola grass in terms of intake of metabolisable energy. Differences in in vitro digestibility of young leaves from vegetative tillers of full sibs, the characteristic on which the genotypes had been selected, were maintained under sward conditions, although differences were reduced.

Author(s):  
Jeannie M Everington ◽  
D I Givens

Compared with stack treatment the literature contains little information on the nutritive value of cereal straws treated with ammonia in “ovens” at elevated temperature. Recently,Mason et al (1987) and Givens et al (1987) have studied in vitro and in vivo respectively the effect on chemical composition, digestibility and energy value of treating barley, wheat and oat straws with ammonia by the oven method. Substantial changes in chemical composition and nutritive value were found as a result of treatment.Reid and Ørskov (1987) have suggested that the dry matter (DM) degradation characteristics of straw as measured by the nylon bag technique provide a good prediction of its nutritive value. The aims of the experiment reported here were therefore to examine using the straws of Mason et al (1987), the effect of oven ammonia treatment on the DM degradation characteristics and to assess whether these parameters could be used to predict the metabolisable energy (ME) content of the straws.


1968 ◽  
Vol 8 (32) ◽  
pp. 295 ◽  
Author(s):  
ID Hume ◽  
M Somers ◽  
NR McKeown

The relationship between the in vivo and in vitro digestibility of leguminous herbage was examined. Further studies were made using in vivo-in vitro digestibility estimations to compare the nutritive value to sheep of two strains (Yarloop and Woogenellup) of subterranean clover. The digestibilities of the main component parts (viz. stem, petiole, leaf, and burr) of the plants of each strain were also estimated in vitro. Digestibility differences between strains and between parts were examined on the basis of the chemical composition of their dry matter. Woogenellup was significantly more digestible than Yarloop, both in vivo and in vitro. Voluntary intake of Woogenellup was also significantly greater than that of Yarloop. The in vitro digestibility of stem did not differ significantly from that of petiole. Nor did the digestibility of leaf differ significantly from that of burr. However, the digestibility of stem and petiole together was greater than that of leaf and burr together. These findings are discussed in relation to their possible biological significance.


1968 ◽  
Vol 8 (32) ◽  
pp. 295
Author(s):  
ID Hume ◽  
M Somers ◽  
NR McKeown

The relationship between the in vivo and in vitro digestibility of leguminous herbage was examined. Further studies were made using in vivo-in vitro digestibility estimations to compare the nutritive value to sheep of two strains (Yarloop and Woogenellup) of subterranean clover. The digestibilities of the main component parts (viz. stem, petiole, leaf, and burr) of the plants of each strain were also estimated in vitro. Digestibility differences between strains and between parts were examined on the basis of the chemical composition of their dry matter. Woogenellup was significantly more digestible than Yarloop, both in vivo and in vitro. Voluntary intake of Woogenellup was also significantly greater than that of Yarloop. The in vitro digestibility of stem did not differ significantly from that of petiole. Nor did the digestibility of leaf differ significantly from that of burr. However, the digestibility of stem and petiole together was greater than that of leaf and burr together. These findings are discussed in relation to their possible biological significance.


Author(s):  
Ali Ameen Saeed ◽  
Saja Intisar Abid

This study was conducted in Nutrition Lab. to investigate the effect of the type and level of substitution of urea with ruminant manure, M (sheep, cow and buffalo) on basis of nitrogen (N) content on the nutritive value of rice straw silage (RSS). Accordingly silages were nominated as, S-RSS, C-RSS and B-RSS. Urea (U) was substituted with dried manure at 6 combinations, 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50 of U:M. Silage samples were prepared by treating chopped straw with pre-treated solution contained 10% low quality debis and 2% urea. Results showed that lower (P˂0.01) DM loss (11.4%) was observed in S-RSS, and with addition of urea only (3.6%).        Samples of S-RSS and C-RSS recorded higher (P˂0.01) Fleig points (Fp) as compared with those prepared by the addition of B-RSS, 60.42, 55.58 and 49.59 respectively. Reduction (P˂0.01) in this parameter was noticed in samples prepared with a combination of 100:0. Aerobic stability (AS) was a reduced (P˂0.01) in samples prepared by addition of S-RSS by 15 and 13 hours in comparison with samples of C-RSS and B-RSS respectively. Samples prepared with combination of 100:0 were prior (P˂0.01) as compared with other samples.        Results also showed an increase (P˂0.01) in in vitro digestibility of organic matter (IVOMD) in samples of S-RSS in comparison with samples prepared by addition of C-RSS and B-RSS, 49.99, 44.59 and 42.77% respectively. Samples prepared with combination of 100:0 recorded lower (P˂0.05) in vitro digestibility of dry matter (IVDMD) as compared with combinations of 70:30 and 60:40 of U: M, 40.52, 45.36 and 45.94% respectively.


1972 ◽  
Vol 23 (1) ◽  
pp. 25 ◽  
Author(s):  
DC Brown ◽  
JC Radcliffe

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy ad libitum intakes and digestibilities of the silages were determined with standardized pairs of Merino wethers. The following chemical characteristics of the silages were measured: nitrogen, ammonia nitrogen, total titratable acids, acetic, propionic, butyric, and lactic acids, total volatiles lost during oven drying, lactic acid as a percentage of the total organic acids, pH, acid pepsin dry matter disappearance, dry matter content, and in vitro digestibility and rate of digestion. When all 20 silages were considered, energy intakes on a body weight basis were significantly related to silage pH (r = 0.55) and rate of in vitro digestion (r = 0.58). When the five legume silages were removed from the analysis and only the 15 grass-dominant silages were considered, dry matter intakes were significantly related to acetic (r = –0.57) and propionic acid (r = –0.55) concentrations. Multiple regression analyses did not significantly increase the accuracy of predicting intake. The results suggested that silage intake was negatively related to the degree of fermentation that occurred during the ensiling process.


2007 ◽  
Vol 2007 ◽  
pp. 233-233 ◽  
Author(s):  
Armin Towhidi ◽  
Farnoush Rostami ◽  
Reza Masoumi

In Iran, Javan (2001) has reported the digestibility some arid rangelands plants by bovine rumen liquor. The determination of in vivo digestibility of wheat straw implies that camel apparently digested poor quality roughages more than cattle and sheep (Cianci et al., 2004). Therefore, It is required to measure the in vitro digestibility of herbages by camel rumen liquor. In province of yazd, nutritive value of 11 different plant species for camel were determined (Towhidi, 2007). The objectives of the current study were to determine 1) the chemical composition, gross energy of the most consuming plant species from rangeland of Semnan province including Seidlitzia rosmarinu, Tamarix tetragyna, Tamarix strica, Halostachys spp, Saudea fruticosa., Alhagi camelorum, Haloxylon ammondendron., Salsola arbescola, Hammada salicornica and, 2) in vitro digestibility of the plants by camel rumen liquor.


2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.


1999 ◽  
Vol 50 (5) ◽  
pp. 871 ◽  
Author(s):  
Paul J. Moughan

The philosophy inherent in developing in vitro digestibility assays for dietary energy and protein is reviewed and an historical account is given of the development of such assays for the pig. General principles to be considered in the development of in vitro digestibility assays are discussed, as are limitations of the in vitro approach. The importance of choosing the most appropriate in vivo measures of digestibility for the evaluation of in vitro assays is stressed. For protein sources that do not contain anti-nutritional factors or plant fibre, ‘true’ ileal digestibility should be the in vivo baseline, while plant proteins should be tested against ‘real’ ileal digestibility. There is a dearth of adequately conducted validation studies for in vitro digestibility assays. It appears that the 3-step (pepsin, pancreatin, Viscozyme) closed in vitro system to allow prediction of organic matter and gross energy digestibility in the pig has particular promise for practical feed evaluation. Similarly based protein digestibility assays may require further development before they can be applied with confidence.


Author(s):  
M.T. Dentinho ◽  
K. Khazaal ◽  
J.M. Ribeiro ◽  
E.R. Ørskov

By using separated values of kinetics of in situ dry matter (DM) degradation or in vitro gas production (Menke and Steingass, 1988) of leguminosae hays, Khazaal et al, (1993) reported high correlation with intake (r= 0.88; r= 0.79) and in vivo DM digestibility (DMD) (r= 0.94; r= 0.88). The aim of the present study was to extend the range of samples used and compare the ability of the 2 stages in vitro digestibility (Tilley and Terry, 1963), the in situ DM degradation or the gas production techniques to predict daily intake (g DM/ kgW0.75) and in vivo DM digestibility (DMD) of 19 leguminous and graminaceous hays fed to sheep.Three harvesting stages (early bloom EB, mid bloom MB or in seed IS) made from lucerne (Medicago sativa), sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum), Rye (Secale cereale), Triticale (Triticale hexaploid), oat (Avena stativa) and a pre-bloom (PB) Italian ryegrass (Lolium multiflorum ). Each hay was fed ad libitum to 4 Merino male sheep and their intake and in vivo DMD recorded. Gas production (ml/ 200 mg DM) or in situ DM degradation (g/ 100 g DM) were determined as described by Khazaal et al, (1993) after 6, 12, 24, 48, 72 or 96 h incubation. Measured gas production or DM degradation values were fitted to the equation p=a+b(l-e-ct)(McDonald, 1981) where p is gas production or DM degradation at time t and a, b and c are constants. For nylon bag the washing loss (soluble fraction) was defined as A, the insoluble but fermentable matter was defined as B=(a+b)-A, and c is the rate of fermentation or degradation (Ørskov and Ryle, 1990).


2010 ◽  
Vol 148 (6) ◽  
pp. 723-733 ◽  
Author(s):  
H. KHALILVANDI-BEHROOZYAR ◽  
M. DEHGHAN-BANADAKY ◽  
K. REZAYAZDI

SUMMARYThe current study was conducted to determine chemical composition, nutrient content and availability, metabolizable energy (ME) content and nutritive value of sainfoin hay for ruminants. Three ruminally cannulated Holstein cows were used forin situandin vivoexperiments, to determine rumen degradability and digestibility of sainfoin hay. Apparent total tract digestibility of nutrients was determined with feeding of sainfoin hay as the sole diet to achieve 10% more than maintenance energy requirements. Six Zandi ewes were used in the palatability experiment. Means for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF) and condensed tannins (CTs) of sainfoin hay were: 940·4 g/kg and 93·43, 12·13, 47·87, 43·33 and 2·13 g/kg DM, respectively.In situeffective degradability of CP and DM were 0·38 and 0·54 g/g with a ruminal outflow rate of 0·05/h, respectively. OM apparent digestibility was in the range of 0·592–0·689, respectively, for Tilley & Terry and total faecal collection assays. ME content of sainfoin hay, according to different methods (gas production,in vitroandin vivodetermined digestible organic matter in dry matter (DOMD)) was in the range 6·87–10·11 MJ/kg DM. Metabolizable protein (MP) content was 483·4 g/kg CP. Sainfoin was more palatable than alfalfa for sheep. It was concluded that sainfoin has a potential use in ruminant rations, especially if environmental conditions are not suitable for alfalfa production.


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