scholarly journals Antivirulence effects of pomegranate peel extracts on most common urinary tract infection pathogens in pregnant women

2016 ◽  
Vol 1 (4) ◽  
pp. 7 ◽  
Author(s):  
Wafaa Sadeq Al-Wazni ◽  
Bashair Sami Hadi
Keyword(s):  
Staphylococcus Aureus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogen ◽  
Pomegranate Peel ◽  
Alcohol Extract ◽  
Inhibition Zones

Objective This study includes the investigation of antibacterial and antivirulence activities of three types of pomegranate peel extracts andthen determines the interaction between the extracts and antibiotic in vitro.Methods The ability of most common isolated bacteria from urinary tract infection (UTI) to produce different virulence factors were testedand the effect of plant extracts on virulence factors were determined; in addition the correlation between extracts and antibiotics wereevaluated by using fractional inhibitory concentrations.Results The inhibition zones diameters of the pomegranate peel extracts against most common isolated bacteria (Staphylococcus aureus andEscherichia coli) increase significantly with increase in concentrations. There is no effect of the extracts on the ability of studied bacteria toproduce hemolysin and protease enzymes, while both studied bacteria lost its ability to produce β-lactamase enzyme after treating with MIC.In addition, extracts were affected largely on adherence activity and biofilm forming ability of tested bacteria. The results found that thepomegranate peel extracts effect alone against pathogenic bacteria was good than they interacted with antibiotics, in most of the results.Conclusion The alcohol extract was the best solvent in its effects on bacterial pathogen and its effect was largely on the ability of the studiedbacteria to form biofilm and adhesion on the epithelial cell. The pomegranate peel extracts were high synergism with some antibioticsagainst pathogenic bacteria.

Enterococcal Virulence Determinants In Urinary Tract Infection Patients

2012 ◽  
Vol 6 (1) ◽  
pp. 14-17
Author(s):  
Jabin Akhter ◽  
Shaheda Anwar ◽  
Sharmeen Ahmed
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Virulence Determinants ◽  
Microtitre Plate ◽  
Microbiological Method ◽  
Enterococcal Infection ◽  
Microtitre Plate Assay ◽  
Positive Frequency

Urinary tract infection caused by Enterococci has become frequent occurrences in health care settings. Currently they emerged with increasing resistance to multiple antibiotics.  Haemolysin, gelatinase and biofilm production are some markers that have been proposed as possible Enterococcal virulence factors. In view of the increasing importance of Enterococcal infection, the present study was designed to isolate and identify the Enterococci to the species level from urine of urinary tract infection patients and to investigate their possible virulence factors. Biofilm was detected on polystyrene microtitre plate to see the adherence of microorganism. Haemolysin production and gelatin hydrolysis detected by standard microbiological method. Fifty nine enterococcal isolates were speciated by conventional microbiological method and examined for their ability to form biofilm by microtitre plate assay. In this study, biofilm formations by Enterococci were found in 83.33% isolates from catheterized and 56.09% from non-catheterized patients. Aong them, E.faecalis & 50% E.faecium produced biofilm. About 43.63% E.faecalis & 10% E.faecium produced haemolysin and only one isolate were found to be gelatinase positive. Frequency of virulence factors (VFs) in combination was observed in this study. Two VFs (haemolysin and biofilm) were observed in 27.11% in combination and 3 VFs ( haemolysinm biofilm and gelatinase) were present in 1.69% isolates. These results suggest that although there may not be an absolute role for individual virulence determinants in infectivity, combinations of factors may play a role in allowing a biofilm infection to be more resistant to therapy.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19361 Bangladesh J Med Microbiol 2012; 06(01): 14-17

The effect of some chemical materials against virulence factors of Candida ssp isolated from UTI patients

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Fatima Abdul Hussein Mejbel
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Germ Tube ◽  
Laboratory Culture ◽  
Candida Spp ◽  
Other Substances ◽  
Chemical Materials

 During the period from September 2016 to December 2017,135 urine samples were collected from urinary tract infection patients attending to AL-Zahraa Hospital in AL-Najaf Governorate. The present study was conducted to isolate and identify Candida spp. isolated from urinary tract infection patients by different methods including direct examination, laboratory culture, biochemical test and by modern techniques (Api Candida kit) and determine the virulence factors phenotypic to Candida spp which involved (biofilm formation,phospholipase and germ tube). The percentage of females to males was as following, female (84) 62.2 % (21) infected and male (51) 37.8% (1) infected with all age categories. The results in this study are explain that is some Candida spp. such as C. albicans, have high susceptible to eugenole follow by phenol and umbellulone. The efficiency of some chemical substances such as (eugenole,umbellulone, and phenol) was evaluated to inhibit the growth of Candida ssp as well as some virulence factors such as biofilm formation,germ tube and phospholipase,which were studied in this research. Statistically analysis results have been significance difference between the results of the substance concentrations and the concentrations of the different other substances.

ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS AGAINST MULTI DRUG RESISTANT URINARY TRACT INFECTION CAUSING ORGANISMS

2021 ◽  
Vol 1 (6) ◽  
pp. 85-95
Author(s):  
Perumal G
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Medicinal Plants ◽  
Tract Infection ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogens ◽  
Drug Resistant ◽  
Antibiotic Drug ◽  
Phytochemical Studies

The present study was isolate Bacterial pathogens form Urinary Tract Infection and identified the Bacterial pathogens from UTI patients. Determination of the antibiotic drug resistant pattern of the isolated pathogenic bacteria using standard antibiotic discs Ampicilin (25μg), Erithromycin (15μg), Chloramphenicol (10μg) Gentamicin (10μg) and Tetracycline (30 μg).The study was carried out, in vitro screening of ethanolic extracts of some medicinal plants against the bacterial pathogens Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Pseudomonas aeruginosawere isolate from the UTI. When compared with standard antibiotic disc selected plants extracts were showed maximum zone of inhibition against all the pathogens. This investigation strongly recommends that phytochemical studies are required to determine the types of compounds responsible for the antibacterial effect of these medicinal plants. Key words: Bacterial pathogens, Antibiotic drug resistant pattern and Medicinal plants

Evaluation of Antibacterial Potential of Ethanol Fruit Peel Extract of Mangifera indica against Isolated UTI Pathogens

2021 ◽  
pp. 3998-4005
Author(s):  
Adeyinka A. Aderinola ◽  
Jane Ejiofor ◽  
Lukmon Ogunjimi ◽  
Akanji A. Murtala ◽  
Oladapo E. Oyinloye
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Fruit Peel ◽  
Gram Negative ◽  
Peel Extract

Effective use of antimicrobial agents for treatment/management of infectious diseases is decreasing due to emergency of multi-drug and cross resistant strains of pathogenic microbes. Medicinal plants are now increasingly used alone or as an adjunct in the management of infectious diseases as a result of their claimed efficacy and safety. This research focused on evaluating the antimicrobial potential of ethanol fruit peel extract of Mangifera indica against isolated Urinary tract infection (UTI) pathogens. Urinary tract infection (UTI) bacterial strains (staphylococcus aureus, escherichia coli and pseudomonas aeruginosa) were isolated from midstream urine of infected students using standard procedures after which the isolated Urinary tract infection pathogens were subjected to antimicrobial susceptibility test by agar-well diffusion method (Cup plate method) and the mean diameter of growth inhibition zones (n=3) of the extract at different concentrations were compared against the controls (sterile water and ciprofloxacin). Ethanol fruit peel extract of Mangifera indica significantly and dose dependently inhibit the growth of all the isolated Urinary tract infection pathogens with E.coli exhibiting the highest inhibition zone ranging from 16.83 to 28.23 mm, this was followed by pseudomonas aeruginosa with inhibition zone of 24.33 mm and staphylococcus aureus least susceptible with 22.63 diameter of zone inhibition. Ethanol fruit peel extract of Mangifera indica demonstrated a dose dependent antimicrobial activity with more pronounced effect exhibited by E. coli suggesting that the extract is more effective against gram negative bacteria despite their permeability barrier, thus suggesting Mangifera indica fruit peel as a potential candidate for the management of bacterial infections especially those caused by gram negative organisms.

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