scholarly journals Detection of genes associated with qualitative characteristics of gluten

2019 ◽  
Vol 14 (3) ◽  
pp. 196-208
Author(s):  
Nagham Majeed Al-Azawi ◽  
Ziyad Asmail Abed ◽  
Mohammed Al-Issawi

The research was aimed at analyzing allelic variants of protein in wheat varieties used in Iraqi bakery and evaluating these varieties via genetic source using grain quality selection. Variety tests were carried out at field experimental station of Russian State Agrarian University - Moscow Timiryazev Agricultural Academy. The analysis of wheat grain quality was made after harvesting in mid August. Allele state of genes controlling the quality of gluten in wheat grain was determined using the PCR method. Samples of Iraqi wheat varieties 12 (soft wheat) and One (durum wheat) are characterized by considerable variation of gluten content and quality. The five varieties whose genotype include an allelic variant of high molecular weight glutenins Glu-D1 5+10 and subunit Glu-A1-2* (Fateh, Tamuz-3, Abighreb-3, Iraq and Maxibak) were also studied. The highest gluten content was from 31.5 % in Iraq to 35.3 % in Fateh variety, while the gluten quality was not below the second group. Five varieties - Farah, AlMurug, Sham-6, Tahadi and Sabirbeg - had unusual combination of the allelic state of Glu-D1 2+12 (which is usually associated with low gluten quality) with a 2* subunit for the Glu-A1 locus, which determines the possibility of improving gluten quality to the wheat varieties.

Author(s):  
V. D. Orekhivskyi ◽  
◽  
A. I. Kryvenko ◽  
S. V. Pochkolina ◽  
◽  
...  

The article investigates the influence of the application of different systems of basic tillage on the quality of winter wheat grain in short crop rotations of the Southern Steppe of Ukraine. It is established that the grain quality of winter wheat in the Southern Steppe of Ukraine is mainly determined by the genetic characteristics of the variety, but also largely depends on the conditions and technologies of cultivation. In winter wheat grain, which is used for food purposes, reserve proteins are important, which in winter wheat determine the baking properties of flour. In drought conditions, when the yield of winter wheat decreases, the protein content in its grain tends to increase. In wet years, on the contrary, there is a reverse pattern. According to experimental studies, wheat varieties have a negative correlation between grain protein content and yield. It is established that during 2016–2020 research shows almost the same pattern of action of different predecessors and systems of basic tillage on the formation of grain quality of winter wheat in arid conditions. It was found that, on average, according to all variants of research, only with the use of the system of tillage-free tillage received grain of winter wheat with a protein content of 12,5%, which met the requirements of the 2nd class. It is recorded that on average over five years of research on all tillage systems, grain of winter wheat with gluten content was obtained, the quality of which corresponded to the 3rd class. Different tillage systems caused a slight impact on the gluten content, which ranged from 20,6% to 21,1%. It is determined that the precursors have a certain effect on the protein content in the grain of winter wheat. The analysis of qualitative indicators showed that on average in five years of research, when growing winter wheat after a pair of black and a pair of green wheat with winter vetch, grain was formed, which in terms of protein content corresponded to the 2nd quality class. More protein was accumulated in winter wheat grain after a pair of black, which was 12,9%. After peas for grain, as well as a pair of green with a mixture of white mustard and peas, received a grain of winter wheat, which was the 3rd quality class. It was found that on average in five years of research, all variants of winter wheat were grown with gluten content, which met the requirements of the 3rd class. Black vapor and green vapor with winter veneer caused the accumulation of gluten at almost the same level with a small increase in the version with winter vetch up to 21,8%. The lowest level of gluten was obtained in the grain of winter wheat after peas per grain, which was 19,7%. In all variants of the experiment, sidereal steam with winter tillage and the use of tillage-free tillage had the best effect on the growth of winter wheat grain quality indicators. The grain of winter wheat was mainly formed in terms of quality, which allows it to be used for food purposes – mainly in the flour-milling and baking industries, as well as for export. It is established that it is important to further study the quality of winter wheat grain and other cereals in short crop rotations of the arid Southern Steppe of Ukraine, especially in climate change.


2018 ◽  
Vol 23 ◽  
pp. 108-113
Author(s):  
O. A. Orlovskaya ◽  
S. I. Vakula ◽  
L. V. Khotyleva ◽  
A. V. Kilchevsky

Aim. T. kiharae (AtAtGGDD, 2n=42) is a source of high protein and gluten content, resistance to many diseases. Сommon wheat lines with the introgression of T. kiharae genetic material were obtained in order to enrich T. aestivum L. gene pool. The aim of this study was to assess the impact of T. kiharae genetic material on the grain quality of T. aestivum/T. kiharae introgression lines. Methods. The composition of the high molecular weight glutenin subunits was analyzed by SDS-PAGE. Evaluation of the most important traits of grain quality (hardness, protein and gluten content, gluten quality) was carried out according to GOST. Results. Сomparative analysis of the composition of high molecular weight glutenin subunits of introgressive lines and their parental forms allowed us to identify lines with novel alleles of Glu-1 loci, specific for T. kiharae. For most of the introgression lines T. aestivum/T. kiharae hardness, protein and gluten content were higher than for parent wheat varieties. Conclusions. Introgression of T. kiharaegenetic material in the genome of common wheat had a positive effect on all studied parameters of grain quality except the gluten quality. Keywords: common wheat, T. kiharae, glutenin, SDS-PAGE, quality of grain.


2020 ◽  
Vol 4 (24) ◽  
pp. 143-151
Author(s):  
S.V. Podgorny ◽  
◽  
O.V. Skripka ◽  
A.P. Samofalov ◽  
S.N. Gromova ◽  
...  

Improving the quality of wheat grain is an important task of agricultural production. In recent years, the production of strong and valuable wheat, necessary for the production of high-quality baking flour, has decreased. In this regard, the study of the quality of grain varieties of winter wheat in specific soil and climatic conditions of the Rostov region is becoming topical. Therefore, the purpose of our research was to study the main indicators of grain quality of varieties of soft winter wheat (protein and gluten content, flour strength, bread size from 100 g of flour) in ecological variety trials to select the best under conditions of the Rostov region. The studies were carried out on the trial fields of the Laboratory of selection and seed production of winter soft wheat of intensive type of the State Scientific Establishment “Agricultural research center «Donskoy»” (Rostov region) in 2014–2016. Seventy-five varieties were studied in the course of the research. Planting dates – optimal for implementation of agricultural and agro-technical measures. Planter – «Wintersteiger Plotseed S.» Seed placement depth – 4–6 cm. Preceding crop – black fallow. Accounting square of fields – 10 m2, double replication. Seeding rate – 4.5 million seeds per hectare. Grain quality was assessed according to the methods of the national standards of the Russian Federation. Such grain quality indicators as gluten content (according to GOST R 54478-2011), protein mass fraction (as required by GOST 108460-91), baking properties of flour (in a laboratory using the remix method) were determined. The article discusses the main indicators of the quality of grain and flour of winter soft wheat varieties in ecological variety testing and compares them. Analysis of the main indicators of the quality of varieties in trials showed that in the south of the Rostov region in 2014–2016 flour strength and gluten content were the most variable indicators. In terms of protein content (> 14.5 %), 8.0 % of the studied varieties were in full compliance with strong wheat standards; by the gluten content (> 28 %) – 7.0 %; by the flour strength (>280 e.a.) – 9.0 %. Five varieties of winter soft wheat: ‘Aksinya’, ‘Tanais’, ‘Asket’, ‘Nakhodka’, ‘Yumpa’ (Russia) with a complex of economically valuable traits are of greater interest for practical breeding in terms of improving grain quality.


2020 ◽  
Vol 107 (3) ◽  
pp. 87-95
Author(s):  
A. M. Zvonar ◽  

Influence of weather conditions of the year and variety features on nitrogen consumption and formation of winter wheat grain quality The article discusses the influence of two factors on the quality of winter wheat grain – the characteristics of the variety and weather conditions of the year. Varietal differences in the nitrogen supply to winter wheat grains were studied. A greater influence of the genetic characteristics of winter wheat varieties than weather conditions on the accumulation of nitrogen in wheat grain was revealed. The dependence of high quality wheat grain on arid weather conditions were confirmed. Varieties such as Arctis and Panonicus have shown more stable indicators of nitrogen content, so the grain of these varieties is the most conditioned. Keywords: nitrogen content, winter wheat, varietal features, protein content, weather conditions.


Author(s):  
K. I. Dokukina ◽  
◽  
A. V. Belinskaya ◽  
T. A. Shelyakin ◽  
L. I. Buriak ◽  
...  

Relevance. The problem of selection improvement of wheat in terms of grain quality is currently relevant. One of the genetic sources for improving grain quality are amphidiploids obtained by hybridizing tetraploid wheat species with the wild related species Aegilops tauschii Coss. (in the world literature they are now referred to as synthetics or synthetic hexaploids - SH). Purpose of the study was to evaluate the grain quality indicators of 29 introgression lines obtained by hybridization of synthetic hexaploids Triticum durum Desf. - Aegilops tauschii Coss. and T.persicum Vav. – Ae.tauschii with bread spring wheat variety Kharkivska 26. Methods. Protein content, gluten content, gluten quality according to gluten deformation index (GDI) in grain grown in different weather conditions - 2015, 2016, 2017, and protein content also in 2020, were analyzed.The analysis of grain quality was carried out in the Laboratory of genetics, biotechnology and grain quality of the Plant Production Institute named after V.Ya. Yuriev of NAAS. The protein content in the grain was determined using an InfraLUM FT-10 device. The gluten quality was determined using an VDK-1 M device. The trait indicators were evaluated on a scale: 0-15 - group III, unsatisfactorily strength; 20-40 - group II, satisfactorily strength; 45-75 - group I, good; 80-100 - group II, satisfactorily weak; 105-120 - group III, unsatisfactorily weak. Evaluation of ecological plasticity and stability was carried out according to the method of Eberhart S.A. and Russel W.A. (1966). Results. The protein content in the grain of the studied lines varied depending on the year conditions. 2017 and 2015 were favorable for the manifestation of the trait being characterized by less precipitation during the period of grain ripening; unfavorable was 2020 - the most humid; intermediate was 2016. The average rate of recurrent variety Kharkivska 26 was 14.6%. The highest protein content was noted in the lines DK 26 and DK 22, respectively, 16.4% and 15.9%. The following lines also exceeded the recurrent variety: DK 4, DK 21, DK 23, DK 30, DK 37, DK 39, DK 44, DK 48, DK 50, DKS 16, DKS 18, DKS 19. The lines of DKS 14 and DKS 15 had the lowest protein content – respectively 12.8% and 13.0%. The lines DK 25, DK 27, DK 39, DK 44 showed relatively high rates of reaction to the year conditions – the plasticity (bi from 1.69 to 2.18). Of these, more stable in the trait manifestation are DK 25 and DK 27: sd is 0.34 and 0.58, respectively. The years 2015 and 2017 were more favorable for gluten accumulation as well as for the protein content, 2016 was unfavorable one. The highest content of crude gluten – from 30.5% to 34.3% – was showed for the lines DK 21, DK 22, DK 26, DK 39, DKS 16, DKS 17, DKS 19 whereas the average grade of Kharkivska 26 was of 27.5%. Of these, DK 21, DK 22, DK 26, DKS 16, DKS 19 were also high in protein. The reaction to year conditions of most lines was characterized by a regression coefficient bi from 0.8 to 1.6 at the rate of Kharkivska 26 of 1.5. The greatest variation showed the line DK 7. The combination of the reaction to year conditions a stable trait manifestation showed DK 22 and DKS 19: bi would be 1.1 and 1.4, sd - 0.00 and 0.01, respectively. In terms of gluten quality, 12 lines are assigned to the first - the best group. Most of the lines, as well as the Kharkivska 26 variety, belonged to group II - satisfactorily weak. Of the samples of the Ist group, the lines DK 47, DKS 12, DKS 13, DKS 18 were more stable on this trait as compared to others. The lines DK 21, DKS 16, DKS 18, in addition to the gluten quality, were also distinguished by the content of protein and gluten; the lines DK 23 and DK 30 – by protein content; DKS 17, DKS 20 – by the gluten content. Analysis of pedigrees shows that among the lines identified for each of the three traits, there are descendants of all synthetics. Therefore, the involvement of synthetics as such is promising for improving grain quality indicators. In our experiments, the protein and gluten content in most lines was higher in the drier years - 2015 and 2017. In the same years, the GDI was usually lower, and therefore closer to better. It should be noted that the parameters by which the lines are selected correspond to the baking properties of the grain. Samples with low protein and low gluten can be valuable for the manufacture of other products - cookies, pita, etc. Thus, the results of our research proved the prospects of using synthetics as a source of grain quality improvement in the selection of spring soft wheat. Perspectives. It is concluded that the use of synthetics is promising as a source of traits for improving the wheat grain quality. The selected lines should be used as sources of grain quality traits for breeding, as well as material for productivity testing in order to include the best of them in variety testing nurseries. The lines with low protein content and weak gluten can be valuable for making specific products - biscuits, pita bread and the like.


Author(s):  
O. A. Orlovskaya ◽  
S. I. Vakula ◽  
L. V. Khotyleva ◽  
A. V. Kilchevsky

Related wild and cultural wheat species are regularly involved for expanding T. aestivum genetic diversity because they contain many valuable genes. We evaluated the effect of the genetic material of tetraploid species of the genus Triticum (T. dicoccoides, T. dicoccum) on the grain quality of introgression lines of spring bread wheat. The composition of the high molecular weight glutenin subunits which play an essential role in the formation of wheat baking properties was identified in the introgression lines of bread wheat and their parental forms. The traits of grain quality (hardness, protein and gluten content, gluten quality) were also evaluated. The lines with Glu-1 loci alleles from wheat relatives T. dicoccoides and Т. dicoccum were selected. It was found that the introgression of alien genetic material into the common wheat genome had a positive effect on the parameters of grain quality such as hardness, protein and gluten content. The lines with Glu-A1 loci alleles from T. dicoccoides and Glu-B1 from T. dicoccum were at the level of a parent wheat variety or of a higher gluten quality. As a result of the research, the new lines of bread soft wheat with high grain quality were found and can be used in the crop breeding.


Author(s):  
H. M. Hospodarenko ◽  
V. V. Liubych ◽  
Ya. S. Riabovol ◽  
I. V. Kochovska

Purpose. Study the formation of yield and grain quality of baking winter wheat of different varieties. Methods. Laboratory, physico-chemical, mathematical and statistical. Results. The grain yield of baking winter wheat varied significantly depending on the variety. On average over two years of research, the highest yield (8.12–8.47 t/ha) was obtained in ‘Colonia’, ‘RGT Reform’, ‘Quebec’, ‘Tobak’, ‘Mescal’ and ‘Emile’ varieties. In ‘Bonanza’ and ‘Rivero’ varieties, this indicator was at the level of 7.48–7.53 t/ha. It should be noted that the stability index was high (0.92–0.98) for growing all varieties. In the favourable 2020 year, the grain yield ranged between 7.62 and 8.80 t/ha, while in the less favourable years it ranged between 7.34 and 8.26 t/ha depending on the variety. On average, over two years of research, the protein content of more than 13.0% was formed by ‘Emile’, ‘Bonanza’, ‘RGT Reform’ and ‘Quebec’ varieties, while in other the varieties it was 12.4–12.9%. The studied varieties of baking wheat had a high stability of protein content (0.98–0.99). Baking winter wheat grain had an average gluten content of 27.7–29.7%, depending on the variety, with high stability. Its highest content (29.5–29.7%) was obtained in ‘Emile’, ‘Quebec’ and ‘RGT Reform’ varieties. The gluten content in grain was directly proportional to the protein content. There is a direct very high correlation between these indicators (r = 0.91 ± 0.001). According to the Zeleny sedimentation test results, flour strength was high only in ‘Quebec’ variety, while in the other varieties it was medium. The grain of ‘Quebec’ and ‘RGT Reform’ varieties corresponds to valuable wheat; ‘Rivero’ and ‘Bonanza’ correspond to a good filler, while the rest of the varieties correspond to a satisfactory filler. The grain of ‘Quebec’, ‘Mescal’, ‘Rivero’ and ‘Colonia’ varieties is medium hard as the hardness was 54.1–59.1 units of instrument. Grain of the other varieties is soft with 48.3–51.1 units of instrument. Grain of ‘RGT Reform’, ‘Rivero’, ‘Emile’ and ‘Tobak’ varieties had the largest (794–812 g/l) grain unit. This indicator in the other varieties was at the level of 760–785 g/l. Flour strength is directly proportional to protein content. There is the direct high correlation between these indicators (r = 0.87 ± 0.002). Correlation between the protein content and the sedimentation index by Zeleny test was direct high (r = 0.79 ± 0.001). However, with grain hardness it was inversely weak, and with the grain unit direct moderate (r = 0.35±0.003). Conclusions. Yield of winter baking wheat varieties was in the range from 7.48 to 8.47 t/ha, protein content varied from 12.4 to 13.8%. Protein content highly correlated with the major baking properties of wheat grain. Baking winter wheat ‘RGT Reform’ and ‘Quebec’ varieties are recommended for stable grain production with good baking properties in the conditions of the Right-Bank Forest-Steppe.


2018 ◽  
Vol 24 (7) ◽  
pp. 627-636 ◽  
Author(s):  
Ravinder Kaushik ◽  
Prince Chawla ◽  
Naveen Kumar ◽  
Sandeep Janghu ◽  
Amit Lohan

Wheat cultivars were treated by four different treatments and their flours were obtained after milling. The quality of the obtained wheat flours was determined using various quality parameters and it was observed that premilling treatments significantly affect the gluten content, wet and dry gluten yield, gluten index, and water absorption capacity of gluten, respectively. Acid-treated wheat flours of all the four wheat varieties (C-306, Raj-3765, PBW-343, and KW-11) showed lower gluten content and quality, whereas conditioning and yeast treatment improve gluten quality as well as its content. Flours obtained after premilling treatments were also utilized for noodle preparation and their sensory and quality parameters were also determined. Also, it was observed that yeast treatment and conditioning improved the noodle quality, whereas acid treatment deteriorates the noodle quality in comparison to tempering. The cultivar PBW-343 produced the best quality noodle among four wheat cultivars. In different milling treatments acid-treated flours of all cultivars showed lowest sensory and quality characteristics, while yeast treatment showed highest.


2018 ◽  
Vol 64 (No. 10) ◽  
pp. 491-497 ◽  
Author(s):  
Holík Ladislav ◽  
Hlisnikovský Lukáš ◽  
Kunzová Eva

This study evaluated how organic manures and mineral fertilizers affect winter wheat grain and straw yields and grain quality properties. The analysed period of the long-term fertilizer experiment was established in Čáslav, Czech Republic, in 1955 and covers the seasons 2011–2014. The fertilizer treatments were: control; farmyard manure (FYM); FYM + P; FYM + K; FYM + PK; FYM + N<sub>1</sub>; FYM + N<sub>2</sub>; FYM + N<sub>1</sub>PK; FYM + N<sub>2</sub>PK and FYM + N<sub>3</sub>PK. The highest grain yields were recorded in the FYM + P and FYM + N<sub>3</sub>PK treatments (8.9 t/ha). The highest straw yields were recorded in the FYM + N<sub>3</sub>PK treatment (6.52 t/ha). The lowest yields were provided in the unfertilized control and FYM treatments. Qualitative parameters were evaluated in the control, FYM and FYM + N<sub>3</sub>PK treatments between the years 2011 and 2013. The best quality of wheat grain was provided by the FYM + N<sub>3</sub>PK treatment. Combination of the farmyard manure with NPK is the best way to achieve high grain yields with good quality and leads to sustainable food production.


Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 268 ◽  
Author(s):  
Diana Tomás ◽  
Wanda Viegas ◽  
Manuela Silva

Wheat is undoubtedly one of the most important crops worldwide and it is essential to study how the distinct varieties answer to heat waves associated with climatic changes, in order to design adequate wheat breeding strategies. To assess high temperature (HT) impact in wheat grain characteristics, seven commercial varieties, which have been recommended for production in Portugal, were submitted for one-week HT treatment ten days after anthesis. Firstly, predicted grain technological quality was determined by giving high scores for all varieties studied, based on the allelic compositions of genes encoding high molecular weight glutenins, granule-bound starch synthase and puroindolines. The effects of HT on transcription levels of those genes were, for the first time, evaluated in distinct wheat genotypes, in comparison with control plants. Finally, protein fraction content in mature grains were also estimated in untreated and treated plants. Immature grains from plants, maintained in control conditions, showed significant intervarietal differences in transcription levels of genes associated with grain quality traits, a variability that was significantly reduced in grains from HT treated plants. On the other hand, the influence of HT in mature grain protein-fractions and in gliadin/glutenin ratios revealed intervarietal diversity, even with opposite effects in some varieties. The present study, therefore, discloses marked variability in parameters associated with flour quality between the wheat varieties analyzed, which are differentially affected by HT treatments, similar to heat waves frequently observed in climate change scenarios.


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