scholarly journals Assessment of vital organ histopathology and plasma oxidative conditions of rainbow trout Oncorhynchus mykiss reared in earthen saltwater pond

2019 ◽  
Vol 14 (3) ◽  
pp. 255-265
Author(s):  
Seyyed Morteza Hoseini ◽  
Behrooz Gharavi ◽  
Yousef Iri
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Thiobarbituric Acid ◽  
Water Salinity ◽  
Hematopoietic Tissue ◽  
Blood Samples ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Earthen Ponds ◽  
Kidney Liver

The aim of this study was to compare gill, kidney, liver and gut histopathology, and plasma antioxidant markers of rainbow trout Oncorhynchus mykiss reared in saltwater earthen ponds in Gomishan, Iran. To this, 10000 fish were distributed in a three-ha earthen pond and 150 fish in three fiberglass tanks (2000L). Blood samples were taken after 3 months rearing with same commercial feed. The source of fish and feed was similar between the saltwater pond and fiberglass tanks. After the 3-month rearing, gill, kidney, liver and gut samples were taken from the pond fish; whereas, blood samples were taken from both the pond and tank fish. There was no significant difference in water temperature, dissolved oxygen and pH between the pond and tanks; however, water salinity and ammonia was higher in the pond compared to the tanks. Plasma superoxide dismutase and glutathione peroxidase activity of the fish in earthen ponds were significantly higher than those fish reared in fiberglass tanks; however, there was no significant in thiobarbituric acid reactive substances between the pond and tanks. The fish had various histopathological symptoms including primary and lamella hyperplasia, lamellar fusion and epithelial lifting. In the kidney section, the fish showed glomerulus shrinkage and/or disappearance, melanomacrophage aggregates and hematopoietic tissue necrosis. These fish showed necrosis and melanomacrophage aggregates in liver and goblet cell hypertrophy in gut. The results suggest that the fish in the earthen pond faced stressful conditions, which might be due to water salinity and ammonia; however, other possible factors, such as pollutants and different feeding regimen must be considered.

Effect of Dietary Ascorbate on the Concentration of Tissue Ascorbic Acid, Dehydroascorbic Acid, Ascorbic Sulfate, and Activity of Ascorbic Sulfate Sulfohydrolase in Rainbow Trout (Oncorhynchus mykiss)

1990 ◽  
Vol 47 (8) ◽  
pp. 1518-1525 ◽  
Author(s):  
Konrad Dabrowski ◽  
Reinhard Lackner ◽  
Cristine Doblander
Keyword(s):  
Ascorbic Acid ◽  
Rainbow Trout ◽  
Vitamin C ◽  
Oncorhynchus Mykiss ◽  
Dehydroascorbic Acid ◽  
Control Group ◽  
High Demand ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Dietary Treatments ◽  
Kidney Liver

The concentrations of ascorbic acid in several tissues of rainbow trout (Oncorhynchus mykiss) are significantly influenced by various dietary treatments. Ascorbic acid was taken up readily by erythrocytes, kidney, liver, intestine, spleen, and brain in fish fed an ascorbate supplemented diet (AA group), the concentration being from 1.5 to 14.8-fold higher than in fish fed a diet lacking ascorbate (control group). In fish fed a diet supplemented with an equimolar amount of ascorbic acid in the form of ascorbic sulfate (AS group) the ascorbic acid concentrations in kidney, intestine, and erythrocytes were significantly elevated above those of the control group. Ascorbic sulfate was found in kidney, liver, and intestine of the AS group, but not in other groups. In fish fed a diet devoid of vitamin C the ascorbic acid concentrations in kidney, liver, intestine, and spleen were signficantly lower than in fasting fish over the same period of time (28 d), suggesting a high demand for vitamin C in an actively feeding animal. Salmonid fish are therefore probably unable to utilize ascorbic sulfate sufficiently to prevent the appearance of vitamin C deficiency, and thus resemble scurvy-prone mammals in this respect.

scholarly journals Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

2012 ◽  
Vol 48 (No. 8) ◽  
pp. 215-220 ◽  
Author(s):  
I. Strunjak-Perovic ◽  
R. Coz-Rakovac ◽  
N. Topic Popovic
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Water Temperature ◽  
Peripheral Circulation ◽  
Ploidy Levels ◽  
Mean Values ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference

The aim of the study was to observe the influence of different ploidy levels in fish on micronucleus occur­rence. Twenty minutes after fertilization, one group of rainbow trout eggs was exposed to water temperatures of 26°C in duration of 20 minutes to induce triploidy. Second group was kept in water temperature of 10°C, which is optimal for development of rainbow trout. The frequency of micronucleated erythrocytes was determined in the peripheral circulation of rainbow trout 67 days (following absorption of the yolk – swim-up stage) and 128 days (fry stage) post fertilization. There was a significant difference (P < 0.001) between frequency of micronucleated erythrocytes of diploid (1.10 ± 0.96‰) and triploid (2.41 ± 1.28‰) fish at swim-up stage. Increased mean values of micronucleus in diploid (1.80 ± 1.57‰) and triploid (5.92 ± 3.80‰) fry were also recorded.

scholarly journals The Effect of Slaughtering Procedures on Blood Spotting in Rainbow Trout (Oncorhynchus Mykiss)

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
George Liviu MIHALCA ◽  
Mihaela TIŢA ◽  
Ana MIHALCA ◽  
Ovidiu TIŢA
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Fish Muscle ◽  
Rigor Mortis ◽  
Visual Appearance ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Colour Measurements ◽  
Significant Difference

Rainbow trout (Oncorhynchus mykiss) was submitted for various slaughter and bleeding procedures to see what effect this would have on blood drainage of the muscles. Results show that the bleeding method is of less importance, while it is the timing that is important. No significant difference in bloodspotting was observed between fish that were bled live by a gill cut or percussive killed and bled by gutting. Most of the drainage of blood in the fish muscle seems to occur within the first hours postmortem, so rigor mortis is of little importance. The visual appearance of the fillet was influenced by number and size of the bloodstains. Colour measurements with Hunter L*, a*, b* did not reveal this. We conclude that a gill cut is not necessarily to obtain bleeding, so the industry can omit this phase and go directly to gutting.

Influence of low temperature acclimation on fate of metabolic fuels in rainbow trout (Oncorhynchus mykiss) hearts

1993 ◽  
Vol 71 (11) ◽  
pp. 2167-2173 ◽  
Author(s):  
John R. Bailey ◽  
William R. Driedzic
Keyword(s):  
Oxygen Consumption ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Temperature Acclimation ◽  
Experimental Conditions ◽  
Intracellular Lipids ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Precipitate Protein ◽  
Fuel Source

Rainbow trout (Oncorhynchus mykiss) were acclimated to 5 and 20 °C. Oxygen consumption of isolated perfused hearts was measured at 5 or 15 °C with either glucose or palmitate as the exogenous fuel source. With glucose as the fuel there was no significant difference in oxygen consumption of hearts from either acclimation group at either temperature. With palmitate as the fuel source, hearts from fish acclimated to and tested at 5 °C had significantly higher oxygen consumption than hearts from fish acclimated to 20 °C and tested at either 5 or 15 °C. Hearts from fish both acclimated to and tested at 5 °C had a higher oxygen consumption with palmitate than when glucose was supplied. This reflects the preference for fatty acid fuels found in cold acclimated muscle tissue, and consequently the amount of oxygen required to utilize fats. Under all experimental conditions, 14CO2 production from either (6-14C)glucose or (1-14C)palmitate could account for less than 0.5% of oxygen consumption. Tissue chemical analysis showed that most of the label from (6-14C)glucose appeared in acid-soluble (glycolytic intermediates, citric acid cycle intermediates, amino acids, etc.) and lipid fractions while most of the label from (1-14C)palmitate appeared in lipid- or acid-soluble or acid precipitate (protein material) fractions. This indicates considerable dilution of exogenous fuels in endogenous pools, which could account for the discrepancy in measured O2 consumption and 14CO2 production. Glucose catabolism was little affected by either acute or chronic changes in temperature other than an increase in glucose incorporation into the glycogen pool. Hearts from fish both acclimated to and tested at 5 °C showed an increased handling of exogenous fatty acids as reflected by elevated rates of catabolism and incorporation into intracellular lipids.

scholarly journals Influence of different meal sizes on growth characteristics of young rainbow trout (Oncorhynchus mykiss Wal).

2013 ◽  
Vol 58 (3) ◽  
pp. 185-193
Author(s):  
Nebojsa Savic ◽  
Milanka Drinic ◽  
Bozo Vazic ◽  
Biljana Rogic
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Ph Value ◽  
Growth Characteristics ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Length Standard ◽  
Aeration System ◽  
Significant Difference ◽  
Banja Luka ◽  
Average Individual

This paper presents the results of the effects of different sizes of meals (80%, 90%, 100%, 110% and 120% regarding the amount recommended by the food producer) on the growth characteristics of young rainbow trout (Oncorhynchus mykiss Wal.) aged from 2 to 3.5 months. The experiment was carried out at the Laboratory of Aquaculture, Faculty of Agriculture, University of Banja Luka for a period of 47 days. In the experiment, there were a total of 500 fish, divided into five groups, and placed in the 55-l aquarium with a constant flow of water and the aeration system. The main parameters of water quality were analyzed every day and they included: water temperature 12.64-12.76?C, content of dissolved oxygen 7.83-8.09 mg/l, saturation of water with oxygen 75.47-77.39% and pH value 7.224-7.268. In the end, the average individual weight and body length (?standard deviation) of young rainbow trout, regarding the experimental groups (EG), were reached: EG80 10.53?2.58 g and 9.94?0.76 cm, EG90 12.14?2.97 g and 10.45?0.86 cm, EG100 13.18?2.91 g and 10.67?0.82 cm, EG110 13.80?3.14 g and 10.82?0.78 cm, EG120 14.58?3.63 g and 10.81?1.11 cm. Statistically highly significant differences were found between the means of experimental groups (p <0,01). The coefficient of condition of young rainbow trout at the end of the experiment was similar in the treatments EG80, EG90, EG100 and EG110 (1.06 to 1.09), and it was the highest in the treatment EG120 (1,15?0,17). Generally, it can be said that increasing of the size of meals up to 10% above the recommended value does not cause significant differences, and if the increase is greater than 10%, statistically significant difference in body weight occurs.

scholarly journals Impact of the Duration of Larval Yolk Sac Absorption on the Development of Rainbow Trout Oncorhynchus mykiss (Walbaum 1792) Fry

2021 ◽  
Vol 79 (3) ◽  
pp. 119-123
Author(s):  
Antonia Kurtela
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Initial Body Weight ◽  
Lower Growth ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Active Feeding ◽  
Lower Growth Rate ◽  
One Way Anova ◽  
Average Individual

Abstract Rainbow trout Oncorhynchus mykiss (Walbaum 1792) larvae that first started active feeding were separated into 3 replicate batches (1A, 1B, 1C) with 100 individuals each, while four days later, the fry that last started active feeding were also distributed in 3 replicate batches (2A, 2B, 2C) with 100 individuals. Four measurements were performed with an interval of four days between the first and second measurements, as well as between the third and fourth measurements, to compare the initial masses of individuals by groups (1ABC and 2ABC) on the same day from the beginning of active feeding. The average initial body weight of the fry per batch in each measurement was generally higher in the first group (1ABC) than in the second group (2ABC). However, when comparing the weight of both groups for the same number of days from the beginning of active feeding, it is noticed that the second group (2ABC) has a higher average individual weight than the first group (1ABC). There was no statistically significant difference (one-way ANOVA, p>0.05) in SGRw between the groups in the first three measurements, while there is a statistically significant difference (one-way ANOVA, p<0.05) in SGRw in the fourth measurement. Group 2ABC has a significantly higher SGRw (5.0064%day−1±0.05394) than group 1ABC (4.7711%day−1±0.01715). This leads to the conclusion that in the second group 2ABC, the backlog of 4 days in mass was compensated. It is also noted that mortality in the first group (1ABC) was only 1%, while in the second group (2ABC) it was 3.7%. Consequently, a higher density of the fry in the first group (1ABC) could have an impact on a lower growth rate.

scholarly journals Oxidative stress and antioxidant defence markers in muscle tissue of rainbow trout (Oncorhynchus mykiss) after vaccination against Yersinia ruckeri

2016 ◽  
Vol 60 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Halyna Tkachenko ◽  
Joanna Grudniewska ◽  
Agnieszka Pękala ◽  
Elżbieta Terech-Majewska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Muscle Tissue ◽  
Yersinia Ruckeri ◽  
Antioxidant Defence ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Derivatives Of

AbstractIntroduction: The goal of this study was to assess the influence of vaccination against enteric redmouth disease on oxidative stress biomarkers and antioxidant defence in the muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against Yersinia ruckeri in the first and second month after immunisation. Material and Methods: Healthy fish were vaccinated orally with inactivated whole cells of a virulent strain of Y. ruckeri. One and two months after immunisation the muscle samples were collected. Results: No significant difference was noted in lipid peroxidation level in either the first or second month after vaccination, while aldehydic and ketonic derivatives of oxidatively modified proteins (OMB) in the vaccinated group were significantly lower in the second month compared to those in the first month after vaccination (P < 0.05). The content of ketonic derivatives of OMB in muscles in the first month after immunisation was higher compared to untreated control. All these culminated in a depletion of glutathione peroxidase (GPx) activity and low level of total antioxidant capacity (TAC). Conclusion: Correlations between catalase activity and lipid peroxidation and TAC confirmed the pivotal role of catalase in antioxidant defence during immunisation. From a broader perspective, it is suggested that immunisation of fish with Yersinia vaccine is associated with induced free radical formation and oxidative stress. Free radicals would therefore be at least partially responsible for the induction of both humoral and cellular elements of the immunity and increased protective immunity against Y. ruckeri infection.

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