scholarly journals Performa Tryptone Bile X-Glucuronide (TBX) yang disuplementasikan dengan Cefotaxime sebagai Medium Selektif Untuk Skrining ESBL-E.coli dari Sampel Lingkungan

2021 ◽  
Vol 49 (1) ◽  
pp. 1-8
Author(s):  
Tati Febrianti ◽  
Sundari Nursofiah ◽  
Novi Amalia ◽  
Dwi Febriyana ◽  
Ratih Dian Saraswati ◽  
...  
Keyword(s):  
Waste Water ◽  
Escherichia Coli ◽  
Selective Medium ◽  
Environmental Sampling ◽  
E Coli ◽  
Sampling Locations ◽  
As Species ◽  
Laboratory Examinations ◽  
Filtration Stage ◽  
Disk Test

Identification of ESBL-E.coli from environment without selective medium will be challenging to do considering that E.coli mixes with various other microorganisms in the environment. This study aimed to determine the performance of TBX Agar supplemented with Cefotaxime as a selective medium for ESBL-E. coli screening from 138 water samples of environmental sampling obtained from rivers, open sewers in the market, poultry slaughterhouses and hospital waste water inlets and outlets around Jakarta. Laboratory examinations were carried out through the filtration stage, inoculation on the TBX Agar supplemented with Cefotaxime medium as well as species confirmation and ESBL with the indol test and double-disk test. The results showed that 87.08% (40-100%) of suspect colonies growing on TBX Agar supplemented with Cefotaxime medium were confirmed as E.coli and 82.51% (12-100%) were confirmed as ESBL-E.coli. However, there was no correlation between TBX Agar supplemented with Cefotaxime performance and sampling locations. Based on the results of the study, it can be concluded that the TBX supplemented with Cefotaxime medium can be used for ESBL-E.coli screening in the environment, but further confirmation is needed using the indole and double-disk tests. Keywords: Escherichia coli, ESBL, TBX Agar suplemented with Cefotaxime Abstrak Identifikasi ESBL-E.coli tanpa medium selektif akan sangat sulit dilakukan pada sampel lingkungan mengingat ESBL-E.coli bercampur dengan berbagai mikroorganisme lainnya. Tujuan penelitian ini yaitu untuk mengetahui performa TBX Agar yang disuplementasi Cefotaxime sebagai medium selektif untuk skrining ESBL-E.coli. Sampel penelitian sebanyak 138 sampel air lingkungan yang diambil dari sungai, saluran pembuangan terbuka di pasar, rumah pemotongan hewan unggas (RPHU) serta inlet dan outlet limbah rumah sakit di sekitar Jakarta. Pemeriksaan laboratorium melalui tahapan filtrasi, inokulasi pada medium TBX Agar dengan suplementasi Cefotaxime serta konfirmasi spesies dan ESBL dengan uji indol dan double-disk test. Hasil menunjukkan bahwa koloni tersangka yang tumbuh pada medium TBX Agar dengan suplementasi Cefotaxime sebanyak 87,08% (40-100%) terkonfirmasi sebagai E.coli dan 82,51% (12-100%) terkonfirmasi sebagai ESBL-E.coli. Namun, tidak ada hubungan antara performa TBX Agar dengan suplementasi Cefotaxime dengan lokasi pengambilan sampel. Berdasarkan hasil penelitian dapat disimpulkan bahwa medium TBX Agar dengan suplementasi Cefotaxime cocok digunakan untuk skrining ESBL-E.coli di lingkungan namun tetap diperlukan konfirmasi lanjut menggunakan uji indol dan double disk test. Kata kunci: Escherichia coli, ESBL, TBX Agar suplemented with Cefotaxime

Impact of Transportation of Feedlot Cattle to the Harvest Facility on the Prevalence of Escherichia coli O157:H7, Salmonella, and Total Aerobic Microorganisms on Hides

2007 ◽  
Vol 70 (1) ◽  
pp. 17-21 ◽  
Author(s):  
A. L. REICKS ◽  
M. M. BRASHEARS ◽  
K. D. ADAMS ◽  
J. C. BROOKS ◽  
J. R. BLANTON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Lower Percentage ◽  
Escherichia Coli O157 ◽  
Sample Collection ◽  
Treatment Groups ◽  
E Coli ◽  
Sampling Locations ◽  
Before And After

Prevalences of Escherichia coli O157:H7, Salmonella, and total aerobic microorganisms were determined on the hides of beef feedlot cattle before and after transport from the feedyard to the harvest facility in clean and dirty trailers. Swab samples were taken from the midline and withers of 40 animals on each of 8 days before and after shipping. After samples were collected, animals were loaded in groups of 10 on upper and lower levels of clean and dirty trailers. Animals were unloaded at the harvest facility and kept in treatment groups for sample collection after exsanguination. Salmonella was found more often on hide swabs collected from the midline than on than samples collected from the withers from animals transported in both clean and dirty trailers. Salmonella was found on significantly more hide swabs collected at harvest from both sampling locations than on those collected at the feedyard, with no differences attributed to the type of trailer. At the feedyard, clean trucks had a lower percentage of Salmonella-positive samples than did dirty trucks before animals were loaded. However, after transport, both clean and dirty trucks had a similar prevalence of Salmonella. There were no differences in Salmonella prevalence on hides collected from animals transported on the top and bottom levels of clean and dirty trucks. E. coli O157:H7 was detected on less than 2% of the samples; therefore, no practical conclusions about prevalence could be drawn. Hides sampled at harvest had higher concentrations of aerobic microorganisms than did hides sampled at the feedyard, and concentrations were higher on the midline than on the withers. Although the prevalences of Salmonella and total aerobic microorganisms increased on hides after transport from the feedyard to the plant, this increase was not related to the cleanliness of the trailers or the location of the cattle in the trailers.

scholarly journals Survival study of escherichia coli o157:h7 in aquatic system of Bangladesh

1970 ◽  
Vol 19 (2) ◽  
pp. 195-201
Author(s):  
Iqbal Kabir Jahid ◽  
Taslima Azad ◽  
Mohammed Ziaur Rahman ◽  
Anowara Begum ◽  
Sirajul Islam Khan ◽  
...  
Keyword(s):  
Waste Water ◽  
Escherichia Coli ◽  
Fluorescent Protein ◽  
Uv Light ◽  
Tap Water ◽  
Aquatic System ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Survival Study ◽  
Survival Pattern

The survival pattern of Escherichia coli O157:H7 was observed in laboratory microcosm with different sources of surface and drinking water using the green fluorescent protein (GFP) as a genetic marker. The water quality was monitored on the basis of bacteriological and physico?chemical parameters. The untreated and filtered water were inoculated with the genetically transformed E. coli O157:H7. The survival pattern was determined by drop plate method observing the green fluorescence of the E. coli O157:H7 colonies under UV light. The survival of E. coli O157:H7 decreased in most of the untreated saline and waste water and higher survival was observed in pond and tap water. The E. coli O157:H7 survived more than 23 days in tap and pond water and less than 20 days in sea, estuarine and waste water. The fluorescent microscopic findings revealed the VBNC state of E. coli O157:H7. The study conclusively proved that the better survival of E. coli O157: H7 depends on the quality of water. Key words: E. coli O157:H7; Aquatic system; Survival study DOI: http://dx.doi.org/10.3329/dujbs.v19i2.8964 DUJBS 2010; 19(2): 195-201

scholarly journals Survival of Escherichia coli in a tropical estuary

2003 ◽  
Vol 21 (1) ◽  
pp. 41 ◽  
Author(s):  
Abirosh Chandran ◽  
A. A. Mohamed Hatha
Keyword(s):  
Escherichia Coli ◽  
Chemical Composition ◽  
Natural Waters ◽  
Negative Impact ◽  
Selective Medium ◽  
Physical Factors ◽  
Estuarine Water ◽  
Biotic Factors ◽  
Growth And Survival ◽  
E Coli

The survival of Escherichia coli in tropical estuarine water has been studied under controlled laboratory conditions using microcosms. The survival has been assessed in terms of various self purifying factors of the natural waters such as biological, chemical and physical factors. The biological factors considered included competition from other microorganisms, predation by protozoa and coliphages. The suitability of the chemical composition of estuarine water has been studied under chemical factors and negative impact of sunlight has been studied under physical factors. The results revealed that sunlight exerted maximum negative impact, followed by biotic factors contained in the estuarine water. However, the chemical composition of the estuarine water is found to be suitable for the growth and survival of E. coli. The injury exerted by each of the above factors was also evaluated by using a selective and non-selective medium in conjunction. It was found that sunlight resulted in 100% injury of the cells as the cells failed to develop in a selective medium. While, sunlight resulted in the extinction of 90% of the E. coli cells within the first two hours of exposure, biotic factors took nearly 24 hours to remove the same amount of population.

scholarly journals Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential

2017 ◽  
Vol 55 (12) ◽  
pp. 3454-3465 ◽  
Author(s):  
Fernanda Esposito ◽  
Miriam R. Fernandes ◽  
Ralf Lopes ◽  
Maria Muñoz ◽  
Caetano P. Sabino ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Zeta Potential ◽  
Metabolic Activity ◽  
Screening Methods ◽  
Phenol Red ◽  
Content Type ◽  
E Coli ◽  
Rapid Dissemination ◽  
Inhibition Zones ◽  
Disk Test

ABSTRACTThe emergence and rapid dissemination of colistin-resistantEscherichia colicarrying the plasmid-mediatedmcr-1gene have created an urgent need to develop specific screening methods. In this study, we evaluated four assays based on the inhibition of MCR-1 activity by EDTA: (i) a combined-disk test (CDT) comparing the inhibition zones of colistin and colistin (10 μg) plus EDTA (100 mM); (ii) reduction of colistin MIC (CMR) in the presence of EDTA (80 μg/ml); (iii) a modified rapid polymyxin Nordmann/Poirel test (MPNP); and (iv) alteration of zeta potential (RZP= ZP+EDTA/ZP−EDTA). We obtained encouraging results for the detection of MCR-1 inE. coliisolates recovered from human, food, and animal samples, using the following assay parameters: ≥3 mm difference in the inhibition zones between colistin disks without and with EDTA; ≥4-fold colistin MIC decrease in the presence of EDTA; RZPof ≥2.5; and the absence of metabolic activity and proliferation, indicated by unchanged color of phenol red in the presence of colistin-EDTA, in the MPNP test. In this regard, the CDT, CMR, RZP, and MPNP assays exhibited sensitivities of 96.7, 96.7, 95.1, and 96.7% and specificities of 89.6, 83.3, 100, and 100%, respectively, for detecting MCR-1-positiveE. coli. Our results demonstrate that inhibition by EDTA and zeta potential assays may provide simple and inexpensive methods for the presumptive detection of MCR-1-producingE. coliisolates in human and veterinary diagnostic laboratories.

Enumeration of stressed cells of Escherichia coli

1979 ◽  
Vol 25 (1) ◽  
pp. 116-118 ◽  
Author(s):  
Aubrey F. Egan
Keyword(s):  
Escherichia Coli ◽  
Acid Treatment ◽  
Selective Medium ◽  
E Coli ◽  
Viable Cells ◽  
Physiological Studies ◽  
Stressed Cells ◽  
K 12

Cells of Escherichia coli K-12 were stressed by heating at 48 °C or by acid treatment at pH 4.2 for periods up to 1 h. The addition of catalase to the selective medium increased the count of heat-stressed cells by 2.3-fold and acid-stressed cells by 4.8-fold. However, these values represented only a small percentage (3% for heat-stressed and 6% for acid-stressed cells respectively) of the population of injured but still viable cells. The addition of mannitol to the selective medium used to count acid-stressed cells did not increase the count. Whilst the presence of H2O2, in media may cause significant errors in the estimation of E. coli in certain situations these errors are unlikely to be significant in physiological studies of populations of cells injured by stress.

Recovery and Survival of Escherichia coli O157:H7 in Reconditioned Pork-Processing Wastewater†

1999 ◽  
Vol 62 (7) ◽  
pp. 731-734 ◽  
Author(s):  
KATHLEEN T. RAJKOWSKI ◽  
EUGENE W. RICE
Keyword(s):  
Escherichia Coli ◽  
Bacterial Growth ◽  
Oxygen Demand ◽  
Selective Medium ◽  
Growth Potential ◽  
Processing Plant ◽  
Escherichia Coli O157 ◽  
Potential Hazard ◽  
E Coli ◽  
Temperature Range

The pathogen Escherichia coli O157:H7 has been recovered from various water sources and food samples. The growth potential of this bacterium in nutrient-limited, reconditioned wastewater from a pork-processing plant was determined over a temperature range of 4 to 46°C. Even though the biological oxygen demand of the wastewater was <2 mg/liter, results of bioassays for assimilable organic carbon and the coliform growth response of the water suggested that sufficient nutrients were present to support limited bacterial growth. A three-strain mixture of E. coli O157:H7 grew over the temperature range of 10.2 to 29.4°C. Bioassays appear to be a good indicator of the ability of this wastewater to support growth of this pathogen. Statistically higher levels of bacterial growth (P < 0.05) were detected on a nonselective medium (tryptic soy agar) than on a selective medium (sorbitol–MacConkey agar), suggesting that stress or injury of the bacterium occurs when the organism is exposed to the nutrient-limited conditions of the wastewater. These results indicate that E. coli O157:H7 can survive and grow in this particular nutrient-limited wastewater, suggesting a potential hazard if this water becomes contaminated with this pathogen.

A Novel Selective Medium for Simultaneous Enrichment of Shiga Toxin–Producing Escherichia coli and Salmonella in Ground Beef

2018 ◽  
Vol 81 (8) ◽  
pp. 1252-1257 ◽  
Author(s):  
JOSEPH EGGERS ◽  
JOELLEN M. FEIRTAG ◽  
ALAN D. OLSTEIN ◽  
JOSEPH M. BOSILEVAC
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Ground Beef ◽  
Performance Standards ◽  
Selective Medium ◽  
Microbiological Analysis ◽  
Department Of Agriculture ◽  
Selective Enrichment ◽  
E Coli ◽  
Inspection Service

ABSTRACT Microbiological analysis of ground beef for contamination by both Salmonella and Shiga toxin–producing Escherichia coli (STEC) is performed by the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS), as part of its Performance Standards Verification Testing program. FSIS has established a zero tolerance for STEC serotype O157:H7 and serogroups O26, O45, O103, O111, O121, and O145 because they are regarded as adulterants. The detection and isolation of these specific serogroups presents a technical challenge necessitating time-consuming and costly laboratory procedures that often exceed the technical capabilities of many small internal and reference laboratories. We describe here a method using a novel STEC and Salmonella selective (SSS) broth that allows for simultaneous selective enrichment of STEC and Salmonella sp., providing isolation and detection from the same broth. The method only involves direct plating from beef enrichments to detect suspect isolates that can be easily confirmed by using immunoassays or PCR, rendering the isolation simpler and less costly than the current described methods. In a side-by-side comparison with modified tryptic soy broth (mTSB), the use of SSS broth resulted in primarily isolating STEC and Salmonella sp., while substantially suppressing the growth of other gram-negative Enterobacteriacae by 90%. Significantly more (χ2 < 3.84) samples containing E. coli O157:H7 and STEC O26, O111, O121, and O145 and a nondifferent (χ2 > 3.84) number of samples containing STEC O103 and O45 were identified when enriching in SSS broth. Coenrichment using six different Salmonella serovars showed numerically greater but not significant (χ2 < 3.84) positive samples by using SSS broth compared with mTSB for a majority of serotypes.

Thermal Destruction of Escherichia coli and Klebsiella pneumoniae in Human Milk

1988 ◽  
Vol 51 (2) ◽  
pp. 132-136 ◽  
Author(s):  
JEFFREY N. MORGAN ◽  
F. JANE LIN ◽  
RONALD R. EITENMILLER ◽  
HAROLD M. BARNHART ◽  
ROMEO T. TOLEDO
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Human Milk ◽  
Thermal Destruction ◽  
Selective Medium ◽  
Selective Media ◽  
E Coli ◽  
High Temperature Short Time ◽  
Z Value ◽  
D Values

A continuous flow high-temperature short-time pasteurization system was used to determine kinetic parameters (D- and z-values) for thermal destruction of the bacterial pathogens, Escherichia coli and Klebsiella pneumoniae, in mature human milk. D-and z-alues of each bacterium were determined from data on survivors enumerated on both selective media, Violet Red Bile agar or MacConkey's, and on a non-selective medium, nutrient agar (NA). For E. coli, D-values were determined at 58, 60, 62 and 64°C. The predicted value of D at 60°C is 31.5 s. The z-value for E. coli is 3.2°C. D-values for K. pneumoniae were determined at 52, 56 and 58°C. Based on these data the predicted value of D at 60°C is 1.3 s. The z-value for K. pneumoniae, is 2.8°C. For both E. coli and K. pneumoniae, counts on NA tend to be higher than on selective media. This is undoubtedly due to the inhibitory nature of the selective media. This also suggests that some degree of thermal injury may occur for each organism.

Fluorescent antibody as a method for the detection of fecal pollution: Escherichia coli as indicator organisms

1973 ◽  
Vol 19 (2) ◽  
pp. 201-206 ◽  
Author(s):  
R. L. Abshire ◽  
R. K. Guthrie
Keyword(s):  
Waste Water ◽  
Escherichia Coli ◽  
Fecal Coliform ◽  
Fluorescent Antibody ◽  
Bacterial Species ◽  
Fecal Pollution ◽  
Indicator Organisms ◽  
E Coli ◽  
Antibody Method ◽  
Fluorescent Antibody Method

A fluorescent antibody method for detection of fecal coliform pollution in water is reported. Detection of Escherichia coli of fecal origin by this method was compared to IMVIC typing and to detection by the E C Broth method. Reliability of this method was tested by reaction of specific E. coli antisera with heterologous bacterial species and with isolates from polluted waste water and unpolluted water and soil sources. This method was found to offer the advantage of greater rapidity over conventional water quality tests, without loss of reliability.

scholarly journals Escherichia Coli in Sewage Sludge - Detection Method / Escherichia Coli W Osadach Ściekowych - Metoda Wykrywania

2014 ◽  
Vol 19 (1-2) ◽  
pp. 79-85 ◽  
Author(s):  
Alicja Machnicka
Keyword(s):  
Escherichia Coli ◽  
Sewage Sludge ◽  
Detection Method ◽  
Bacterial Flora ◽  
Dry Weight ◽  
Suspended Solid ◽  
Selective Medium ◽  
Gram Negative ◽  
E Coli ◽  
Biochemical Identification

Abstract Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry suspended solid, - preparation averaged, test of sample and resuscitation of bacteria, - making dilutions, - enrichment and differentiation in chromogenic-selective medium, - enumerating the amount of cfu E. coli in 1 g of a dry weight, - optionally, the biochemical identification

Sign in / Sign up

Export Citation Format

Share Document