The biologic behavior of squamous cervical carcinoma after neoadjuvant therapy (NAT) according to immunohistochemical expression of E-cadherin and CD44v6

Abstract.Graflund M, Sorbe B, Karlsson M. Immunohistochemical expression of p53, bcl-2, and p21 WAF1/CIP1 in early cervical carcinoma: Correlation with clinical outcome.The objective of this study was to assess the value of p53, bcl-2, and p21WAF1/CIP1 immunoreactivity as predictors of pelvic lymph node metastases (LNM), recurrences, and death due to the disease in early stage (FIGO I-II) cervical carcinomas. FIGO stage, type of histopathology, and tumor grade were also evaluated in this series of patients treated by radical hysterectomy (Wertheim-Meigs) between 1965 and 1990. A total of 172 patients were included. A tumor was regarded as positive when more than 30% of the neoplastic cells exhibited immunoreactivity. Positive immunostaining was found in 8.9% for p53, in 43.5% for bcl-2, and in 25.0% for p21WAF1/CIP1. None of them was able to predict LNM or clinical outcome. Presence of LNM, tumor recurrence, and death from disease were significantly associated with the FIGO stage (P = 0.014, P = 0.009, and P = 0.001, respectively). The 5-year cancer-specific survival rate was 91.6% and the overall survival rate was 90.5%. It was concluded that immunohistochemically detected p53, bcl-2, and p21WAF1/CIP1 appeared to be of no predictive value with regard to LNM, tumor recurrences, or long-term survival in early cervical carcinomas.

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Immunohistochemical expression of E-cadherin and β-catenin in ameloblastomas and tooth germs

Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology ◽

10.1016/j.tripleo.2009.10.032 ◽

2010 ◽

Vol 109 (3) ◽

pp. 425-431 ◽

Cited By ~ 18

Author(s):

Karuza Maria Alves Pereira ◽

Bruna Aguiar do Amaral ◽

Bruna Rafaela Martins dos Santos ◽

Hébel Cavalcanti Galvão ◽

Roseana de Almeida Freitas ◽

...

Keyword(s):

Immunohistochemical Expression ◽

Tooth Germs ◽

E Cadherin

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Levels of Circulating TIMP-2 and MMP2-TIMP2 Complex Are Decreased in Squamous Cervical Carcinoma

Obstetrics and Gynecology International ◽

10.1155/2010/179351 ◽

2010 ◽

Vol 2010 ◽

pp. 1-3 ◽

Cited By ~ 3

Author(s):

Talvensaari-Mattila Anne ◽

Turpeenniemi-Hujanen Taina

Keyword(s):

Cervical Carcinoma ◽

Matrix Metalloproteinase 2 ◽

Healthy Controls ◽

Matrix Degradation ◽

Serum Samples ◽

Study Population ◽

The Mean ◽

Natural Tissue ◽

Squamous Cervical Carcinoma

Background. The role of matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in matrix degradation and metastasis has been described in various tumors. Their action is inhibited by their natural tissue inhibitor molecules TIMP-1 and -2.Methods. The study population consisted of 12 squamous cervical carcinoma patients and 27 healthy volunteer control patients. MMP-9, MMP-2-TIMP-2 complex, TIMP-1, and TIMP-2 were analyzed from serum samples using enzyme-linked immunoassay (ELISA).Results. The mean levels of serum TIMP-2 and of MMP-2-TIMP-2 complex were higher in healthy controls compared to patients with a malignant tumor. Serum TIMP-2 values decreased significantly from healthy controls (median 323 g/l, range 305–342 g/l) to malignant (median 136 g/l, range 120–151 g/l) squamous cervical carcinoma patients . Also, serum proMMP2-TIMP2 complex values decreased from control patients to squamous cervical carcinoma patients .Conclusion. This paper shows that the levels of circulating TIMP-2 and that of MMP-2-TIMP-2 complex are lower in squamous cervical carcinoma patients than in healthy women.

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Pretreatment serum hemoglobin level as a predictive factor of response to neoadjuvant chemotherapy in patients with locally advanced squamous cervical carcinoma: A preliminary report

Gynecologic Oncology ◽

10.1016/j.ygyno.2005.07.079 ◽

2005 ◽

Vol 99 (3) ◽

pp. S187-S191 ◽

Cited By ~ 8

Author(s):

Luca Fuso ◽

Simona Mazzola ◽

Francesco Marocco ◽

Annamaria Ferrero ◽

Daniela Dompè ◽

...

Keyword(s):

Neoadjuvant Chemotherapy ◽

Cervical Carcinoma ◽

Predictive Factor ◽

Preliminary Report ◽

Locally Advanced ◽

Hemoglobin Level ◽

Pretreatment Serum ◽

Squamous Cervical Carcinoma ◽

Response To Neoadjuvant Chemotherapy

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Hexokinases 2 promoted cell motility and distant metastasis by elevating fibronectin through Akt1/p-Akt1 in cervical cancer cells

Cancer Cell International ◽

10.1186/s12935-021-02312-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Qian Chen ◽

Lu Li ◽

Xian Liu ◽

Qian Feng ◽

Yanru Zhang ◽

...

Keyword(s):

Cervical Cancer ◽

Cell Motility ◽

Cancer Cells ◽

Cervical Carcinoma ◽

Distant Metastasis ◽

Tumor Metastasis ◽

Sequencing Analysis ◽

Mmp9 Expression ◽

Cervical Cancer Cells ◽

Squamous Cervical Carcinoma

Abstract Background Hexokinases 2 (HK2) is a member of the hexokinases, linking with malignant tumor growth and distant metastasis. However, evidence regarding the potential role of HK2 in regulating cell motility and tumor metastasis during the cervical cancer malignant progression remains limited. Methods In vitro migration and invasion assay, in vivo metastasis experiments were performed to detect the effective of HK2 on regulating cell motility and tumor metastasis in cervical cancer cells. RNA-Seq was performed to explore the potential molecules that participate in HK2-mediated cell motility and tumor metastasis in cervical cancer cells. The correlation between HK2 and Akt1, p-Akt1, FN1 expression in cervical cancer cells and human squamous cervical carcinoma (SCC) samples was verified in this study. Results In this study, cervical cancer cells with exogenous HK2 expression exhibited enhanced cell motility and distant metastasis. Transcriptome sequencing analysis revealed that fibronectin (FN1) was significantly increased in HK2-overexpressing HeLa cells, and the PI3K/Akt signaling pathway was identified by KEGG pathway enrichment analysis. Further studies demonstrated that this promotion of cell motility by HK2 was probably a result of it inducing FN1, MMP2 and MMP9 expression by activating Akt1 in cervical cancer cells. Additionally, HK2 expression was altered with the changing of Akt1/p-Akt1 expression, implying that HK2 expression is also modulated by Akt1/p-Akt1. Moreover, the positive correlation between HK2 and Akt1, p-Akt1, FN1 expression in human squamous cervical carcinoma (SCC) samples was verified by using Pearson correlation analysis. Conclusions This study demonstrated that HK2 could activate Akt1 in cervical cancer cells, subsequently enhancing cell motility and tumor metastasis by inducing FN1, MMP2 and MMP9 expression. There likely exists an interactive regulatory mechanism between HK2 and Akt1 during the malignant process of cervical cancer.

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