scholarly journals Identification and functional analysis of a testis‑biased gene encoding serine/arginine‑rich protein in silkworm, Bombyx mori

2019 ◽  
Vol 71 (4) ◽  
pp. 639-645
Author(s):  
Li-Ying Zhang ◽  
Juan Li ◽  
Xing-Fu Zha
Keyword(s):  
Amino Acids ◽  
Bombyx Mori ◽  
Sequence Similarity ◽  
Similarity Analysis ◽  
Specific Gene ◽  
Rt Pcr ◽  
Gene Encoding ◽  
Fundamental Process ◽  
Sequence Similarity Analysis ◽  
Silkworm Bombyx Mori

Spermatogenesis is a fundamental process in sexual reproduction. In this study, we cloned a 716-bp cDNA of a testis-biased gene in Bombyx mori, named as BmRS-TS, which encodes a polypeptide of 164 amino acids, containing 26.7% arginine and serine residues. Sequence similarity analysis showed that BmRS-TS is a lepidopteran-specific gene. Results of RT-PCR and Western analysis revealed that BmRS-TS was expressed predominantly in the testis. Immunohistochemistry assay showed that the BmRS-TS protein was mostly located in primary spermatocytes. Moreover, knockdown of BmRS-TS by RNA interference (RNAi) showed that the morphology of the mature sperm was abnormal and that sperm bundles were broken up. Our results suggest that BmRS-TS plays an important role in silkworm spermatogenesis and provide some clues for understanding the mechanism that underlies spermatogenesis, which can be used as a reference for other lepidopterans.

scholarly journals Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

BMB Reports ◽  
2010 ◽  
Vol 43 (7) ◽  
pp. 480-484 ◽  
Author(s):  
Seung-Won Park ◽  
Seok-Woo Kang ◽  
Tae-Won Goo ◽  
Seong-Ryul Kim ◽  
Gwang-Gill Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Real Time ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Specific Gene ◽  
Rt Pcr ◽  
Tissue Specific ◽  
Tissue Specific Gene ◽  
Silkworm Bombyx Mori

Characterization of a gene encoding prohibitin in silkworm, Bombyx mori

Gene ◽  
2012 ◽  
Vol 502 (2) ◽  
pp. 118-124 ◽  
Author(s):  
Zhengbing Lv ◽  
Xufang Zhang ◽  
Lili Liu ◽  
Jian Chen ◽  
Zuoming Nie ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Gene Encoding ◽  
Silkworm Bombyx Mori

A New Approach for DNA Sequence Similarity Analysis based on Triplets of Nucleic Acid Bases

2010 ◽  
Vol 2 (4) ◽  
pp. 1-11
Author(s):  
Dan Wei ◽  
Qingshan Jiang ◽  
Sheng Li
Keyword(s):  
Nucleic Acid ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Sequence Similarity ◽  
Similarity Analysis ◽  
Biological Sequence ◽  
Nucleic Acid Bases ◽  
New Approach ◽  
Characteristic Distribution ◽  
Sequence Similarity Analysis

Similarity analysis of DNA sequences is a fundamental research area in Bioinformatics. The characteristic distribution of L-tuple, which is the tuple of length L, reflects the valuable information contained in a biological sequence and thus may be used in DNA sequence similarity analysis. However, similarity analysis based on characteristic distribution of L-tuple is not effective for the comparison of highly conservative sequences. In this paper, a new similarity measurement approach based on Triplets of Nucleic Acid Bases (TNAB) is introduced for DNA sequence similarity analysis. The new approach characterizes both the content feature and position feature of a DNA sequence using the frequency and position of occurrence of TNAB in the sequence. The experimental results show that the approach based on TNAB is effective for analysing DNA sequence similarity.

scholarly journals Characterization of Pepper leafroll chlorosis virus, a New Polerovirus Causing Yellowing Disease of Bell Pepper in Saudi Arabia

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 318-326 ◽  
Author(s):  
A. Kamran ◽  
L. Lotos ◽  
M. A. Amer ◽  
M. A. Al-Saleh ◽  
I. M. Alshahwan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Sequence Similarity ◽  
Aphis Gossypii ◽  
Aphid Transmission ◽  
Bell Pepper ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Growing Seasons ◽  
Arable Weeds ◽  
Sequence Similarity Analysis

During the growing seasons of 2014 through 2016, a total of 336 leaf samples from bell pepper (showing leafroll and interveinal yellowing) and arable weeds were collected from Riyadh region, Saudi Arabia. The use of a polerovirus generic reverse transcription (RT)-PCR assay confirmed their presence in the bell pepper samples. Sequencing of the generic amplicon revealed high similarity (87.6 to 98.1% in nt) with four poleroviruses; Tobacco vein distorting virus, Pepper vein yellows virus, Pepper yellows virus, and Pepper yellow leaf curl virus. To further characterize one of these isolates (105D), a larger part of the genome (∼1,300 nt) spanning approximately from the 3′ end of ORF2 to the middle of ORF3, was amplified and sequenced. Blasting the resulting sequence revealed the low amino acid and nucleotide identity percentages in the coat protein and movement protein partial genes with viruses deposited in GenBank. Next-generation sequence was used to acquire a larger part of the genome, which resulted in the reconstruction of isolate 105D’s partial genome (5,496 nt). Sequence similarity analysis revealed the presence of a divergent polerovirus isolate belonging to a new species that was tentatively named Pepper leafroll chlorosis virus (PeLRCV). Using a specific RT-PCR assay for this isolate confirmed the presence of this new viral species in the symptomatic peppers. Aphid transmission experiments showed that PeLRCV is vectored by Aphis gossypii and that it can infect at least five out of the 15 different plants species tested. Based on our findings, PeLRCV is a new member of genus Polerovirus in the family Luteoviridae.

Application of rpoB sequence similarity analysis, REP-PCR and BOX-PCR for the differentiation of species within the genus Geobacillus

2008 ◽  
Vol 46 (3) ◽  
pp. 395-401 ◽  
Author(s):  
C. Meintanis ◽  
K.I. Chalkou ◽  
K. Ar. Kormas ◽  
D.S. Lymperopoulou ◽  
E.A. Katsifas ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Similarity Analysis ◽  
Sequence Similarity Analysis ◽  
Rpob Sequence
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