scholarly journals The bonding of anesthetics and antibiotics on the carboxymethylcellulose

2007 ◽  
Vol 61 (4) ◽  
pp. 203-207
Author(s):  
Branka Rodic-Grabovac ◽  
Radana Djudjic ◽  
Nadezda Iliskovic
Keyword(s):  
Chemical Modification ◽  
Water Solution ◽  
Active Material ◽  
Maximum Amount ◽  
Degree Of Substitution ◽  
Biologically Active ◽  
Procaine Hydrochloride ◽  
Nacl Solution ◽  
Cellulosic Material ◽  
Gentamicin Sulphate

The preparation of biologically active material is one of the most interesting trends in the chemical modification of cellulose. The possibility of obtaining biologically active cellulosic material by sorption of procaine hydrochloride and gentamicin sulphate on CMC was investigated in this paper. The sorption of therapeutics on CMC samples with different degree of substitution was carried out in water solutions of anesthetic and antibiotic, while the desorption was done in 0.95% NaCl solution. The amount of bonded and released therapeutics was determined by UV spe-ctrophotometry. It was found that degree of substitution of car-boxymethylcellulose and concentration of chemotherapeutic water solution have significant influence on the amount of bonded anesthetic and antibiotic. The maximum amount of bonded chemotherapeutic was 72.15 mg of procaine hydrochloride and 165.49 mg of gentamicin sulphate per gram CMC. In both cases the amounts of bonded chemotherape-utics correspond to the concentration of local anesthetic and antibiotic preparation that are used in therapeutic purposes.

scholarly journals Influence of the structure of ceftazidime on obtaining biologically active cellulosic bandage

2017 ◽  
Vol 71 (1) ◽  
pp. 35-40
Author(s):  
Branka Rodic-Grabovac ◽  
Radana Djudjic ◽  
Pero Sailovic
Keyword(s):  
Hydrophobic Interactions ◽  
Water Solution ◽  
Drug Loading ◽  
Drug Carriers ◽  
Solution Concentration ◽  
Physiological Solution ◽  
Maximum Amount ◽  
Biologically Active ◽  
Agar Diffusion Test

Biologically active fibers as drug carriers have improved characteristics in comparison with conventional medical therapies. Cellulosic fibers as hydrophilic and biocompatible, nontoxic and eco-friendly make a good polymer matrix for obtaining biologically active fibers. Current investigations in this area show that the features of these fibers depend on the type of carrier as well as the drug structure. Loading drugs on the fiber carrier is accomplished by ionic bonding between ionized drugs and the groups fixed on the fiber (by ion exchange) or loosely adsorption on the fiber through hydrophobic interactions. These interactions can be achieved between hydrophobic parts of the drug and the fiber carrier or among the hydrophobic drugs bonded on the fiber. Prevailing mechanism of ionized drug bonding on the carrier is ionic, although different hydrophobic interactions can contribute the drug loading to varying degrees. In this paper oxidized cellulose (OC) with different carboxylic group content is obtained by selective oxidation and used for chemical bonding of antibiotic ceftazidime. In its structure this antibiotic has aminothiazole ring and pyridine ring in the side chains of cephem nucleus. Ceftazidime has two carboxylic groups and also great number of electron donors and acceptors. Due to these groups and structures ceftazidime is able to form multiple chemical bonds i. e. interactions with oxidized cellulosic bandage. The bonding was performed in antibiotic water solution concentration of c=3,4?10-3 mol/L at room temperature (22 ? 1?C), while desorption was performed in physiological solution. The amounts of bonded and released antibiotic were determined spectrophotometrically in UV range. Maximum amount of bound drug (0,0243 mg/g) was obtained during the sorption on the oxidized bandage with 2,276 mmol/g COOH and the maximum amount of released drug was 0,0238 mmol/g. Antimicrobial activity of the samples with bonded ceftazidime was tested in vitro against Staphylococcus aureus, Bacillus subtilis i Escherichia coli by agar diffusion test. The bigest zone of inhibition was obtained for Escherichia. The paper studies the influence of ceftazidime chemical structure, pH of solution in which sorption is performed and sorption duration, on the amount of bonded drug. It was established that the drug bonding was achieved by ionic bonds and the hydrogen bonds of the drug functional groups with oxidised cellulose bandage. Also it was found that buffering of the drug solution, in which bonding is performed, can increase the amount of ceftazidime bonded on the fiber.

ISOLEMENT ET QUELQUES CARACTERISTIQUES D'UNE GONADOTROPHINE URINAIRE DE MÉNOPAUSE HOMOGÈNE A L'ELECTROPHORÈSE

1960 ◽  
Vol XXXV (II) ◽  
pp. 225-234 ◽  
Author(s):  
R. Bourrillon ◽  
R. Got ◽  
R. Marcy
Keyword(s):  
Sialic Acid ◽  
Histological Study ◽  
Active Material ◽  
Biologically Active ◽  
New Method ◽  
Female Rats ◽  
Mouse Uterus ◽  
Highly Active ◽  
Zone Electrophoresis ◽  
Human Menopausal Gonadotrophin

ABSTRACT A new method for preparation of Human Menopausal Gonadotrophin involves successively alcoholic precipitation, kaolin adsorption and chromatography on ion exchangers. A highly active material is obtained which corresponds to 1 mg per litre of urine and has an activity of 1 mouse uterus unit at a dose of 0.003 mg. This gonadotrophin possesses both follicle stimulating and luteinizing activities in hypophysectomized female rats, by histological study. It contains 13 % hexose, 10% hexosamine and 8.5 % sialic acid. A further purification, by zone electrophoresis on starch, gives a final product, biologically active at 0.001 mg, which behaves as an homogenous substance in free electrophoresis with mobility −4.76 × 10−5 at pH 8.6.

Characterisation of Persistent Anti-Xa Activity Following Administration of the Low Molecular Weight Heparin Enoxaparin Sodium (Clexane)

1994 ◽  
Vol 72 (02) ◽  
pp. 275-280 ◽  
Author(s):  
David Brieger ◽  
Joan Dawes
Keyword(s):  
Molecular Weight ◽  
Enoxaparin Sodium ◽  
Antithrombin Iii ◽  
Anticoagulant Activity ◽  
Active Material ◽  
Biologically Active ◽  
Size Exclusion ◽  
Low Molecular Weight ◽  
Exclusion Chromatography ◽  
Liver And Kidney

SummaryIt is widely reported that persistent anti-Xa activity follows administration of low molecular weight heparins. To identify the effectors of this activity we have injected 125I-labelled Enoxaparin sodium into rabbits and subsequently analysed the circulating radiolabelled material and anti-Xa activity by affinity and size exclusion chromatography. Antithrombin III-binding material derived from the injected drug was responsible for all the anti-Xa amidolytic activity. At early times after injection additional anticoagulant activity which was largely attributable to tissue factor pathway inhibitor was measured by the Heptest clotting assay after removal of glycosaminoglycans from plasma samples. Small radiolabelled fragments, including penta/hexasaccharide with affinity for antithrombin III, were detectable in the circulation 1 week later, and sulphated oligosaccharides persisted for 3-4 weeks. Significant quantities of radiolabel remained in the liver and kidney several weeks post-injection; these organs may sequester some of the injected drug and give rise to circulating biologically active material by degradation and secretion of catabolic products into the plasma.

scholarly journals The productivity and mass of internalss of quails are for actions feed addition of «Proenzym»

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
N. M. Baluh
Keyword(s):  
Live Weight ◽  
Maximum Amount ◽  
Feed Additives ◽  
Biologically Active ◽  
Poultry Feed ◽  
Feed Additive ◽  
Internal Organs ◽  
Basic Diet ◽  
Experimental Group ◽  
Different Doses

Established that the largest distribution combined with feed additives which include a number of biologically active substances. As an alternative to antibiotics, the use of which recently banned their production and significantly reduced feeding system is used four groups of drugs, enzymes, probiotics, prebiotics and аcidifiers. The largest number of feed additives used in poultry feed. Therefore, to improve the consumption and use of feed efficiency, obtaining maximum animal performance ensured a high level of balanced nutrition using the same enzyme–probiotic supplements. It is to include such feed additives «Proenzym» which consists of live bacteria genus Bacillus subtilis and an enzyme tsellyulaza. What prevents gastrointestinal diseases, improves digestion and absorption of essential nutrients intake. Therefore, the aim was to study the effect of feeding different doses feed additive «Proenzym» in the quail Full–feed and its impact on productivity and internal organs. For the experiment selected 200 day old Estonian quail breed. Of these, the principle of analogues formed four groups of 50 goals each. The duration of the experiment – 56 days. The first (control) group during the entire experiment received a basic diet, and quail research groups in addition to the basic diet fed different doses of feed additives. For the effects of various doses feed additive had a positive effect on live weight quails in 42 and 49–day age. Yes, most live weight at the end of the experiment were females and males second experimental group 10.8% and 8.3%, which in addition to full–feed introduced minimum dose «Proenzym» The introduction of the new maximum amount of feed factor had a significant impact on the weight of internal organs, quails second group for the actions of study drug increased the mass of the lungs and liver, respectively, 0.6 g and 3.4 g, and gallbladder in 4 experimental group 0.16 g. Therefore, we can conclude that the diets of quails best was the maximum amount of feed additive.

Transport of biologically active material in laser cutting

1988 ◽  
Vol 8 (6) ◽  
pp. 562-566 ◽  
Author(s):  
Martin Frenz ◽  
Fabien Mathezloic ◽  
Michael H. S. Stoffel ◽  
Adrian D. Zweig ◽  
Valerio Romano ◽  
...  
Keyword(s):  
Laser Cutting ◽  
Active Material ◽  
Biologically Active

Nano-Cellulose as Promising Biocarrier

2008 ◽  
Vol 47-50 ◽  
pp. 1286-1289 ◽  
Author(s):  
Michael Ioelovich ◽  
Oleg Figovsky
Keyword(s):  
Chemical Modification ◽  
Effective Treatment ◽  
High Power ◽  
Biologically Active ◽  
Liquid Media ◽  
Lipid Layer ◽  
Nano Cellulose ◽  
Nano Size ◽  
Active Substances ◽  
Modified Cellulose

Main purpose of this paper is to describe the method for preparation of the reactive nanocellulose biocarrier and to discuss some its biomedical and cosmetic applications. The method for preparation of the biocarrier was proposed that includes steps of controlled depolymerization of initial cellulose; structural and chemical modification in order to graft various biologically active substances to cellulose particles and high-power mechanical disintegration of the modified cellulose particles in liquid media. As a result, the dispersion of the bioactive nano-cellulose was obtained. Due to nano-size, particles of bioactive nano-cellulose capable clean skin's pores, open them and penetrate through the lipid layer and epidermis within the skin strata. This effect of the biocarrier can be employed at the development of advanced types of biomedical and cosmetics remedies used for gentle care and effective treatment of the skin.

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