scholarly journals Antibacterial activity chemical composition relationship of the essential oils from cultivated plants from Serbia

2011 ◽  
Vol 65 (5) ◽  
pp. 583-589 ◽  
Author(s):  
Nemanja Stankovic ◽  
Ljiljana Comic ◽  
Branislava Kocic ◽  
Dejan Nikolic ◽  
Tatjana Mihajilov-Krstev ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Cultivated Plants ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Broth Microdilution Method

The antibacterial effects of essential oils from Serbian cultivated plants, Thymus vulgaris L. (Lamiace) and Lavandula angustifolia L. (Lamiace) on different bacteria were investigated, with an emphasis on an antibacterial activity-chemical composition relationship. Essential oil was obtained from airdried aerial parts of the plants by hydrodistillation for 3 h using a Clevenger-type apparatus. The essential oil analyses were performed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) systems. The main constituents of thyme oil were thymol (59.95%) and p-cymene (18.34%). Linalyl acetate (38.23%) and linalool (35.01%) were main compounds in lavender oil. The antibacterial activity of the essential oils samples was tested towards 5 different bacteria: laboratory control strain obtained from the American Type Culture Collection and clinical isolates from different pathogenic media. Gram negative bacteria were represented by Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 43895 and Salmonella enteretidis ATCC 9027 while researched Gram positive strains were Bacillus cereus ATCC 8739 and Staphylococcus aureus ATCC 25923. A broth microdilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Essential oils from thyme have been found to have antimicrobial activity against all microorganisms tested, with a range of MIC values from 0.025 to 0.10 l/ml and MBC values from 0.05 to 0.78 l/ml. Lavender oils demonstrated MIC values from 0.025 to 0.20 l/ml and MBC values from 0.05 and 0.78 l/ml. Reference antibiotic tetracycline was active in concentrations between 0.025 and 0.05 l/ml. The Gram-positive bacteria were more sensitive to the essential oil of thyme, while Gram-negative bacteria were more sensitive to the essential oil of lavender. Essential oils from thyme and lavender may be used at low concentrations for prevention and treatment of infective diseases in animals and humans caused by pathogenic bacterial species.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Espeletia nana

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
Gram Positive Bacteria

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).

Antibacterial Activity and Chemical Composition of the Essential Oil of Grammosciadium platycarpum Boiss. from Iran

2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Two Samples

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.

scholarly journals Chemical composition, antimicrobial and antioxidant potential of the essential oil of Guarea kunthiana A. Juss

2017 ◽  
Vol 78 (1) ◽  
pp. 53-60 ◽  
Author(s):  
J. A. Pandini ◽  
F. G. S. Pinto ◽  
M. C. Scur ◽  
C. B. Santana ◽  
W. F. Costa ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Properties ◽  
Antioxidant Potential ◽  
General Point ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Broth Microdilution Method

Abstract The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay. The GC-MS analyses allowed identifying 13 constituents, representing 96.52% of the essencial oil composition. The main compounds identified were α-zingiberene (34.48%), β-sesquiphellandrene (22.90%), and α-curcumene (16.17%). With respect to the antimicrobial activity, the essential oil was effective against all the microorganisms tested, except for the bacteria E. coli and K. pneumoniae, which were resistant to the action of the oil. From a general point of view, Gram-positive bacteria were more susceptible to the action of the essential oil than Gram-negative bacteria. The essential oil exhibited antioxidant potential.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Ruilopezia bracteosa

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Libia Alarcón ◽  
Alexis Peña ◽  
Judith Velasco ◽  
José Gregorio Baptista ◽  
Luis Rojas ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Gram Negative Bacteria ◽  
Broth Microdilution ◽  
Gram Positive ◽  
Gram Negative ◽  
Agar Diffusion

The essential oil from the leaves of Ruilopezia bracteosa was obtained by hydrodistillation, and analyzed by GC-MS. Eighteen components, which made up 99.6% of the oil were identified, the most abundant being β-myrcene (34.2%), α-pinene (24.3%), 7-epi-α-selinene (9.1%), β-pinene (8.5%) and 6,9-guaiadiene (4.4%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using broth microdilution and disk agar diffusion methods. MIC values found presented significant differences between both methods, which may be due to diffusion factors.

scholarly journals In vitro Antibacterial Activity of Libanotis montana Essential Oil in Combination with Conventional Antibiotics

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Dragoljub L. Miladinović ◽  
Budimir S. Ilić ◽  
Tatjana M. Mihajilov-Krstev ◽  
Jovana L. Jović ◽  
Marija S. Marković
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Hierarchical Cluster ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method

The chemical composition and antibacterial activity were examined of Libanotis montana Crantz subsp. leiocarpa (Heuff.) Soó. (Apiaceae) essential oil. Gas chromatography and gas chromatography/mass spectrometry were used to analyze the chemical composition of the oil. The antibacterial activity was investigated by the broth microdilution method against thirteen bacterial strains. The interactions of the essential oil and three standard antibiotics: tetracycline, streptomycin and chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods: principal components analysis and hierarchical cluster analysis. Sesquiterpene hydrocarbons were the most abundant compound class in the oil (67.2%), with β-elemene (40.4%) as the major compound. The essential oil exhibited slight antibacterial activity against the tested bacterial strains in vitro, but the combinations L. montana oil-chloramphenicol and L. montana oil-tetracycline exhibited mostly either synergistic or additive interactions. These combinations reduced the minimum effective dose of the antibiotics and, consequently, minimized their adverse side effects. In contrast, the association of L. montana essential oil and streptomycin was characterized by strong antagonistic interactions against Escherichia coli ATCC 25922. In the PCA and HCA analyses, streptomycin stood out and formed a separate group.

Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. (Geraniaceae)

2010 ◽  
Vol 64 (3) ◽  
Author(s):  
Zorica Stojanović-Radić ◽  
Ljiljana Čomić ◽  
Niko Radulović ◽  
Milan Dekić ◽  
Vladimir Ranđelović ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Palmitic Acid ◽  
Gram Negative Bacteria ◽  
Broth Microdilution ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Microdilution Method ◽  
Broth Microdilution Method

AbstractThe present study gives results of chemical composition analyses and antimicrobial activity testing of three Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. Essential oils were obtained by hydro-distillation from air-dried entire plants and analyzed by GC and GC-MS. A total of 209 different compounds were identified: 162 for E. cicutarium, 107 for E. ciconium, and 79 for E. absinthoides. Antimicrobial activity (broth microdilution method) of the oils was screened against a panel of Gram positive and Gram negative bacteria and a number of fungi. Moderate susceptibility of all tested strains was observed. Determined MIC values were 0.156–5 mg mL−1 (bacterial strains) and 0.039–0.325 mg mL−1 (fungal strains). Major component of the most active oil, palmitic acid, was also tested for activity together with stearic and myristic acids.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Baccharis latifolia Pers. and B. Prunifolia H. B. &K. (Asteraceae)

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Luis B. Rojas ◽  
Tulia Díaz ◽  
Juan Carmona ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Enterococcus Faecalis ◽  
Mass Spectra ◽  
Retention Indices ◽  
Gram Positive ◽  
Gram Positive Bacteria

The essential oils from leaves of Baccharis latifolia and B. prunifolia collected in January 2006 were analyzed by GC/MS. The yields of oils extracted by hydrodistillation were 0.27 and 0.29% for B. latifolia and B. prunifolia, respectively. Sixteen (B. latifolia) and twenty nine (B. prunifolia) components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The identified products may be divided into four different groups: monoterpenes (9.0% B. latifolia; 43.9% B. prunifolia), oxygenated monoterpenes (0.8% B. latifolia; 5.4% B. prunifolia), sesquiterpenes (20.4% B. latifolia; 45.9% B. prunifolia) and oxygenated sesquiterpenes (69.8% B. latifolia; 1.9% B. prunifolia). The oils showed antibacterial activity only against Gram positive bacteria, with MIC values for Staphylococcus aureus (ATCC 25923) of 80 μg/mL (B. latifolia) and Enterococcus faecalis (ATCC 29212) of 90 μg/mL and 260 μg/mL (B latifolia and B. prunifolia, respectively).

scholarly journals Chemical Composition and Antibacterial Activities of Eight Plant Essential Oils from Morocco against Escherichia coli Strains Isolated from Different Turkey Organs

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hassna Jaber ◽  
Asmaa Oubihi ◽  
Imane Ouryemchi ◽  
Rachid Boulamtat ◽  
Ali Oubayoucef ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectroscopy ◽  
Plant Essential Oils ◽  
E Coli ◽  
Gas Chromatography Mass Spectroscopy

The aim of the present study was to determine the chemical composition of eight plant essential oils and evaluate their antibacterial activity against Escherichia coli strains isolated from different turkey organs. The essential oils were extracted by hydrodistillation and analyzed using gas chromatography-mass spectroscopy. All essential oil yielded high in a range between 2.2 and 3.12%. Gas chromatography-mass spectroscopy (GC-MS) revealed that the major constituents of Thymus vulgaris, Ocimum basilicum, Artemisia herba-alba, and Syzygium aromaticum oils were thymol (41.39%), linalool (37.16%), camphor (63.69%), and eugenol (80.83%), respectively. Results of the E. coli sensitivity evaluated by the standard antimicrobial sensitivity method varied depending on the organ of isolation. Similarly, the essential oils antimicrobial activity determined by the disc diffusion method varied all along within the organs of isolation. T. vulgaris essential oil showed the highest effective antibacterial activity against E. coli isolated from the throat with an inhibition zone diameter value of up to 23.33 mm. However, all the essential oils showed antibacterial activity and the MIC and MBC values were in the range of 1/3000 to 1/100 (v/v) and the ratios MBC/MIC were equal to 1. In conclusion, this study showed that the essential oils could be promising alternatives to overcome E. coli multiresistance in turkey.

scholarly journals Potential Antimicrobial and Chemical Composition of Essential Oils from Piper caldense Tissues

2017 ◽  
Vol 60 (3) ◽  
Author(s):  
Dayane Silva Rocha ◽  
Janete Magali Da Silva ◽  
Daniela Maria Do Amaral Ferraz Navarro ◽  
Claúdio Augusto Gomes Camara ◽  
Camila Soledade De Lira ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Biological Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Major Chemical ◽  
Antibacterial Potential

The essential oils from leaves, stems and roots of Piper caldense were analyzed by GC-MS. The antibacterial potential of the oils was evaluated against gram-negative bacteria and gram-positive bacteria. The major chemical constituents that were identified from various parts of this plant were α-cardinal, α-muurolol, tujopsan-2-β-ol and δ-cadiene in the leaves, valencene, pentadecane, elina-3,7-11-dieno α-terpineol in the roots and terpine-4-ol, α-terpineol, α-cadinol 2-β-ol in the stems. Tissue oils showed antibacterial activity against the bacteria tested except for Enterococcus faecalis. This is the first report of the biological activity and chemical composition essential oil of P. caldense.

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