scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Baccharis latifolia Pers. and B. Prunifolia H. B. &K. (Asteraceae)

2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Luis B. Rojas ◽  
Tulia Díaz ◽  
Juan Carmona ◽  
...  

The essential oils from leaves of Baccharis latifolia and B. prunifolia collected in January 2006 were analyzed by GC/MS. The yields of oils extracted by hydrodistillation were 0.27 and 0.29% for B. latifolia and B. prunifolia, respectively. Sixteen (B. latifolia) and twenty nine (B. prunifolia) components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The identified products may be divided into four different groups: monoterpenes (9.0% B. latifolia; 43.9% B. prunifolia), oxygenated monoterpenes (0.8% B. latifolia; 5.4% B. prunifolia), sesquiterpenes (20.4% B. latifolia; 45.9% B. prunifolia) and oxygenated sesquiterpenes (69.8% B. latifolia; 1.9% B. prunifolia). The oils showed antibacterial activity only against Gram positive bacteria, with MIC values for Staphylococcus aureus (ATCC 25923) of 80 μg/mL (B. latifolia) and Enterococcus faecalis (ATCC 29212) of 90 μg/mL and 260 μg/mL (B latifolia and B. prunifolia, respectively).

2008 ◽  
Vol 3 (3) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Antonio Morales ◽  
Luis Rojasa ◽  
Tulia Díaz ◽  
...  

The essential oil from the leaves of Baccharis trinervis (Lam.) Pers., collected in May 2006, was analyzed by GC/MS. A yield of 0.2% oil was obtained by hydrodistillation. Forty three components were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components were germacrene D (20.4%), limonene (15.4%), δ-cadinene (5.2%), β-caryophyllene (4.8%), α-pinene (4.5%) and bicyclogermacrene (4.0%). The essential oil showed antibacterial activity against the important human pathogenic Gram positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212, with MIC values of 80 and 200 μg/mL, respectively.


2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).


Bionatura ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 908-912
Author(s):  
Jesús E. Velásquez ◽  
Luis B. Rojas-Fermín ◽  
Judith Velasco ◽  
Rosa L. Aparicio ◽  
Alfredo N. Usubillaga ◽  
...  

This work presents a comparative study of the chemical composition of the essential oils obtained from two varieties of Centrolobium paraense (orinocense EO o and paraense EO p) from Venezuela. GC analyzed the oils, and the constituents were identified by GC-MS and retention indices. Eighteen compounds were identified in EO o, which made up 88.9 % of the oil, but only sixteen compounds were identified in EO p, which made up 95.5 % of the total oil. β-caryophyllene (35.1 %) and -humulene (35.3 %) are the main constituents of EO p. The main constituents of EO o are α-humulene (24.8 %), β-caryophyllene (14.3 %), caryophyllene oxide (18.33 %), and humulene epoxide II (16.86 %). The biological activity of both oils was assayed. They were found to be equally active against Staphylococcus aureus and Enterococcus faecalis, with MIC of 100 and 600 μL/mL respectively. This is the first report describing the chemical composition of the essential oil of these species and their antibacterial activity.


2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Flor D. Mora ◽  
Yesenia L. Rojas ◽  
Viviana González ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
...  

The composition and antimicrobial activity of the essential oil from Verbesina negrensis Steyerm. leaves is reported. Analysis was performed by GC/MS. Major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). The essential oil showed in vitro activity against the Gram-positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 (MIC 500 and 350 μL/mL respectively).


2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.


2008 ◽  
Vol 3 (6) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Teresa Díaz ◽  
Flor D. Mora ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Luis B. Rojas ◽  
...  

The chemical constituents of the essential oil obtained by hydrodistillation from the leaves of Calycolpus moritzianus (O. Berg) Burret, syn Psidium caudatum Mc Vaught, collected in November 2006 in Mérida State, Venezuela, were identified by GC-MS analysis. Thirty components (91.1% of the sample) were identified, of which the seven major ones were β-caryophyllene (21.9%), α-pinene (10.9%), viridiflorol (9.7%), β-selinene (6.1%), α-copaene (6.3%), α-selinene (5.3%) and γ-eudesmol (5.1%). The oil was found to have antibacterial activity against Staphylococcus aureus ATCC (6538) and Enterococcus faecalis ATCC (29212), with MIC values of 60 μg/mL and 180 μg/mL, respectively.


2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501
Author(s):  
Jorge A. Pino ◽  
Mauricio Gaviria ◽  
Juana Quevedo-Vega ◽  
Laura García-Lesmes ◽  
Clara E. Quijano-Celis

The chemical composition of the volatile compounds from the leaves of Galinsoga parviflora Cav. (Asteraceae) from Colombia was studied by GC and GC/MS. Eighty-eight volatile compounds were identified, of which the major ones were (Z)-3-hexen-1-ol (21.7%), β-caryophyllene (12.4%), and 6-demethoxy-ageratochrome (14%). The leaf oil presented antimicrobial activities against the Gram-positive bacteria Staphylococcus aureus and Bacillus cereus.


2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.


2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
José Moreno ◽  
Rosa Aparicio ◽  
Judith Velasco ◽  
Luis B Rojas ◽  
Alfredo Usubillaga ◽  
...  

The hydrodistilled oil from the fruits of Bursera tomentosa, obtained in 0.2% yield, was analyzed by GC-MS. Nine components were identified, which made up 99.3% of the oil. The most abundant constituents were cis-ocimene (47.6%), n-nonane (28.2%) and germacrene-D (11.1%). The oil showed antibacterial activity against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212) and Salmonella typhi (CDC 57), with MIC values of 80 μg/mL, 120 μg/mL and 100 μg/mL, respectively.


2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800
Author(s):  
Ratchuporn Suksathan ◽  
Siriwoot Sookkhee ◽  
Somboon Anuntalabhochai ◽  
Sunee Chansakaow

The essential oils from rhizomes of five Hedychium species, H. coronarium, H. neocarneum, H. flavescens, H. speciosum and H. stenopetalum (Zingiberaceae), were obtained by hydrodistillation and analyzed by capillary GC and GC/MS. Sixty components were identified and percentage oil yields from the fresh plants ranged from 0.06-0.17 % (v/w). All rhizome oils were rich in terpenes, especially monoterpenes (75.0-95.9 %). The most common compounds in the rhizome oils of Hedychium were β-pinene, linalool and 1,8-cineole. The essential oils were tested against four bacterial strains. They showed moderate to weak activity against Gram-positive bacteria (inhibition zone 25-13 mm, MIC 0.3-8.3 mg/mL, MBC 0.6-8.3 mg/mL).


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