scholarly journals Spectrophotometric investigation of the Fe(III)-disulphonate hydroquinone complex

2005 ◽  
Vol 70 (4) ◽  
pp. 651-659
Author(s):  
Mirjana Obradovic ◽  
Snezana Mitic ◽  
Snezana Tosic ◽  
A.N. Pavlovic
Keyword(s):  
Stability Constant ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Relative Stability ◽  
Time Stability ◽  
Acid Media ◽  
Influence Of Temperature ◽  
Complex Dependence ◽  
Indigo Blue

Iron(III) form an indigo-blue complex with the disulphonated product of hydroquinone (K2S2Hy) in acid media with an absorption maxima at 600 nm. The time stability of the complex, dependence of the complex absorbance on pH and the influence of temperature and solvent were followed on the basis of spectrophotometric measurements. Using the Job, mole ratio and Henry?Franck?Ostwald methods, the composition and relative stability constant of this complex, in 80 vol.% ethanol as solvent, were determined (1:1;log??293 = 3.37). A new spectrophotometric method for the determination of iron has been developed and the calibration curve is linear in the concentration range from0.65 to 6.45 ?g cm-3. The effects of foreign ions on the determination of iron were investigated in order to assess the selectivity of the method. The method was applied for the determination of Fe(III) in the natural juice of beet.

scholarly journals New Spectrophotometric Method for Determination of Cadmium

2009 ◽  
Vol 6 (s1) ◽  
pp. S496-S500
Author(s):  
K. S. Parikh ◽  
R. M. Patel ◽  
K. N. Patel
Keyword(s):  
Stability Constant ◽  
Spectrophotometric Method ◽  
Basic Medium ◽  
Buffer Solution ◽  
Solution Ph ◽  
Yellow Colour ◽  
Maximum Absorbance ◽  
The Stability

The reagent 2-hydroxy-4-n-butoxy-5-bromopropiophenone thiosemicarbazone (HBBrPT) has been used for the determination of Cd(II) by using spectrophotometric method. The reagent HBBrPT gave an intense yellow colour with Cd(II) solution in basic medium. The maximum absorbance was observed at 440 nm, in basic buffer solution (pH 10.00). The molor absorptivity and Sandell’s sensitivity of Cd(II)-HBBrPT complex were 4035 mol-1cm-1and 0.02765 μg cm-2respectively. The stability constant of 1:2 Cd(II)-HBBrPT complex was 8.46×106. The effect of various iron was also studied.

scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

Oxidative Coupling: A Tranquil Approach for Determination of Selexipag by Visible Spectrophotometry

2018 ◽  
Vol 34 (6) ◽  
pp. 3112-3117 ◽  
Author(s):  
Giri Prasad Gorumutchu ◽  
Venkata Nadh Ratnakaram
Keyword(s):  
Regression Analysis ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Oxidative Coupling ◽  
Coupling Reaction ◽  
Oxidative Coupling Reaction ◽  
Pulmonary Arterial

The present study is a first report on development of a spectrophotometric method for determination of selexipag (used to cure pulmonary arterial hypertension) in bulk and tablet formulation and its validation. The basis of the proposed method is formation of a chromophore (of λ max 600 nm) in presence of acidic ferric chloride by oxidative coupling reaction between selexipag and MBTH (3-methylbenzo-thiazolin-2-one hydrazone) solution. Regression analysis (r > 0.999) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (5 – 30 μg mL-1). As per the existing guidelines of ICH, various parameters of the method were tested for validation. Low values of R.S.D. (< 2 %) were observed indicating that the proposed method is reproducible, accurate and precise.

scholarly journals Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

2011 ◽  
Vol 8 (4) ◽  
pp. 1528-1535 ◽  
Author(s):  
F. Nekouei ◽  
Sh. Nekouei
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Ion Associate ◽  
Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

scholarly journals Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of Liposomal/Proliposomal Turbidity

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Neelkant Prasad ◽  
Roshan Issarani ◽  
Badri Prakash Nagori
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Uv Detection ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Precision And Accuracy ◽  
Clear Solution

A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glipizide in presence of lipid turbidity is described to avoid false estimation due to diffraction by turbidity. UV detection was performed at 230 nm, 225 nm, and 235 nm, and the calibration curve was plotted between resultant of absorbance of [230 nm − (225 nm + 235 nm)/2] and concentration of analyte. The calibration curve was linear over the concentration range tested (1–20 μg/mL) with limit of detection of 0.27 μg/mL and limit of quantification of 0.82 μg/mL. Percent relative standard deviations and percent relative mean error, representing precision and accuracy, respectively, for clear as well as turbid solutions, were found to be within acceptable limits, that is, always less than 0.69 and 0.41, respectively, for clear solution and 0.65 and 0.47, respectively, for turbid solution. Conclusively, our method was successfully applied for the determination of glipizide in clear as well as turbid solutions, and it was found that the drug analyte in both types of solutions can be detected from the same calibration curve accurately and precisely and glipizide entrapped in the liposomes or in proliposomal matrix was not detected.

Determination of Sucralose by Controlled UV Photodegradation Followed by UV Spectrophotometry

2013 ◽  
Vol 96 (3) ◽  
pp. 603-606
Author(s):  
Mohd Idris ◽  
V Jayathirtha Rao ◽  
Deepak Middha ◽  
Sudhir Kumar Shukla ◽  
Tulsidas Ramachandra Rao Baggi
Keyword(s):  
Correlation Coefficient ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Artificial Sweeteners ◽  
Uv Spectrophotometry ◽  
Medium Ph ◽  
Accuracy And Precision ◽  
Uv Photodegradation

Abstract A simple and time-efficient UV spectrophotometric method was developed for the determination of sucralose in artificial sweeteners. The procedure is based on the UV spectrophotometric determination of the photodegraded product at 270 nm in alkaline medium (pH 12). LOD for this method was 0.02 g/L, and the calibration curve was found to obey Beer's law in the concentration range of 0.1 to 1.2 g/L, with a correlation coefficient (R2) value of 0.9977. The proposed method was studied for accuracy and precision.

scholarly journals NOVEL UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF TERIFLUNOMIDE IN TABLET DOSAGE FORM

2019 ◽  
pp. 81-84
Author(s):  
VISHWAS T. S. ◽  
GURUPADAYYA B. M.
Keyword(s):  
Linear Relationship ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Dosage Form ◽  
Current Work ◽  
Validation Parameters ◽  
Line Equation ◽  
Tablet Dosage ◽  
Ich Guideline

Objective: The current work is intended towards the development of a novel, simple, and precise UV spectrophotometric method for the estimation of teriflunomide (TEF) present in the marketed formulation. Methods: Acetonitrile was used as asolvent and the absorbance of the drug was measured at the absorbance maxima of TEF, UV 284 nm. Results: Calibration curve plotted in concentration range 5-10 µg /ml exhibited excellent linear relationship with line equation y = 0.0858x-0.0223 and r2 value of 0.9996. The method was found to comply all the validation parameters as per the ICH guideline indicating the sensitivity of the method towards analyte. Conclusion: The method can be used satisfactorily for the routine analysis of TEF present in marketed formulation.

scholarly journals Development of an Assay Method for Simultaneous Determination of Ciprofloxacin and Naproxen by UV Spectrophotometric Method

1970 ◽  
Vol 4 (1) ◽  
pp. 84-90 ◽  
Author(s):  
Rajia Sultana Nijhu ◽  
Yeakuty Marzan Jhanker ◽  
Kumar Bishwajit Sutradhar
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Regression Line ◽  
Assay Method ◽  
Distilled Water ◽  
Maximum Absorbance ◽  
Key Words Ciprofloxacin ◽  
The Media

The PDF for this article was updated on 18/11/2011.YM Jhanker and KB Sutradhar were added as authors to this paper on 18/11/2011.The Objective of the present study was to develop a simple, reproducible and economical spectrophotometric method for simultaneous determination of Ciprofloxacin and Naproxen. Both Drugs obey Beer's law in the range of 1-5μg/ml concentration. The Standard curve of Ciprofloxacin and Naproxen in the media of 0.1N HCl, Distilled water and Phosphate buffer are obtained by plotting absorbance versus concentration where calibration curve was found to be linear (R2>0.99) with optimum value of standard error for the entire analytical medium used. The plot of the residuals was normally distributed around the regression line, which reflects the accuracy of the method. For simultaneous determination of Ciprofloxacin and Naproxen, the linear plot was found in the media of distilled water and 0.1N HCl acid. The maximum absorbance was found in the media of distilled water for Ciprofloxacin at wavelength 278nm and for Naproxen at 228nm. The calibration curve was to be linear for Ciprofloxacin as R2 >0.99 and for Naproxen as R2 >0.99.The maximum absorbance was found in the media of 0.1N HCl for Ciprofloxacin at wavelength 277nm and for Naproxen at 228nm. The calibration curve was to be linear for Ciprofloxacin as R2 >0.98 and for Naproxen as R2 >0.99. Key words: Ciprofloxacin; Naproxen; Spectrophotometric analysis; Simultaneous equation method. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8876 SJPS 2011; 4(1): 84-90

scholarly journals Spectrophotometric determination of neomycin sulphate in tablets form via reaction with ninhydrin reagent

2019 ◽  
Vol 10 (2) ◽  
pp. 1392-1396 ◽  
Author(s):  
Khalaf F Alsamarrai ◽  
Menaa Abdulsalam Al-Abbasi ◽  
Eman Thiab Alsamarrai
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Basic Medium ◽  
Optimal Conditions ◽  
Limit Of Quantification ◽  
Maximum Absorbance ◽  
Ninhydrin Reagent

A new, sensitive, simple and cheap spectrophotometric method for the determination of Neomycin Sulphate (NEO) in pharmaceutical forms has been developed. The method is based on the reaction between NEO and NIN in basic medium. The maximum absorbance was at 574 nm. The conditions affecting the reaction were optimized. Under the optimal conditions, the calibration curve was linear over the range of 0.0002-0.0011 mol/L. The limit of detection and limit of quantification were 5.423×10-6 mol/L, and 1.643×10-5 mol/L, RSD% of seven replicate was 0.8217- 0.8321% and Rec% was between 99.2168-100.8857%. The proposed method was successfully applied to the determination of NEO tablets form.

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