scholarly journals Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase

2018 ◽  
Vol 83 (5) ◽  
pp. 549-559 ◽  
Author(s):  
Xin Wang ◽  
Yu Shi ◽  
Zi Ni ◽  
Zhao Li ◽  
Jia Bao
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Volume Ratio ◽  
Degradation Efficiency ◽  
Immobilized Laccase ◽  
Degradation Rates ◽  
Experimental Parameters ◽  
Polycyclic Aromatic ◽  
Ph Conditions ◽  
Better Than

The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 volume ratio of soil to PAH solution. Subsequently, the adsorption?cross-link composite immobilization method was applied to immobilize laccase derived from fungi onto nylon net and chitosan, respectively. These two kinds of immobilized laccase were used in the degradation of pyrene (Pyr) and benzo[a]pyrene (BaP), and their degradation efficiencies under different temperature and pH conditions were investigated. Consequently, the optimal laboratory experimental parameters were determined as follows: first, compared with free laccase, the degradation rates of Pyr and BaP by immobilized laccase increased by around 10?30 %; second, the degradation efficiency of chitosan as a carrier of immobilized laccase was much better than that of nylon net as a carrier of the immobilized laccase; finally, when the temperature was set at 40?C and the pH was set at 4, the degradation efficiency achieved by immobilized laccase was the best.

scholarly journals Tracing historical changes, degradation, and original sources of airborne polycyclic aromatic hydrocarbons (PAHs) in Jilin Province, China, by Abies holophylla and Pinus tabuliformis needle leaves

2021 ◽  
Author(s):  
Zhao Wang ◽  
Xiangzi Jin ◽  
Han Yeong Kaw ◽  
Zakia Fatima ◽  
Maurizio Quinto ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Dry Weight ◽  
Air Pollutant ◽  
Jilin Province ◽  
General Tendency ◽  
Coal Consumption ◽  
Pinus Tabuliformis ◽  
Degradation Rates ◽  
Polycyclic Aromatic

Abstract Due to their wide distribution and availability, plant leaves can be considered interesting candidates as biomonitoring substrates for the evaluation of atmospheric pollution. In addition, some species can also retain historical information, for example, related to environmental pollution, due to their leaf class age. In this study, the content of polycyclic aromatic hydrocarbons (PAHs) in Abies holophylla and Pinus tabuliformis needle samples in function of their class age has been investigated to obtain information regarding the degradation constant for each PAH under investigation (α values ranging from 0.173 to 1.870) and to evaluate the possibility to correlate the presence of PAHs in needles with some important pollution environmental factors. Considering air pollutant variables registered in Jilin Province, significant correlations (at 95% confidence level) have been found between coal consumption per year and anthracene contents in needles, while fluorene, phenanthrene, and anthracene resulted correlated with coal consumption. Furthermore, it has been demonstrated that the total PAH concentration in needles, for both species, increased with their age (from 804 to 3604 ng g− 1 dry weight), showing a general tendency to accumulate these substances through years. PAH degradation rates increased instead with molecular complexity. This study could be considered a first trial to obtain historical environmental information by pine needles biomonitoring.

scholarly journals Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant

2020 ◽  
Vol 31 (3) ◽  
pp. 14
Author(s):  
Ali A. Taha ◽  
Nahida J. Hameed ◽  
Farah Hamed Ali
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Trametes Versicolor ◽  
Free Enzyme ◽  
The Other ◽  
Chitosan Beads ◽  
Immobilized Laccase ◽  
Mediator System ◽  
Polycyclic Aromatic ◽  
The Stability

Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.

scholarly journals Tracing historical changes, degradation, and original sources of airborne polycyclic aromatic hydrocarbons (PAHs) in Jilin Province, China, by Abies holophylla and Pinus tabuliformis needle leaves

2021 ◽  
Author(s):  
Zhao Wang ◽  
Xiangzi Jin ◽  
Han Yeong Kaw ◽  
Zakia Fatima ◽  
Maurizio Quinto ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Dry Weight ◽  
Air Pollutant ◽  
Jilin Province ◽  
General Tendency ◽  
Coal Consumption ◽  
Pinus Tabuliformis ◽  
Degradation Rates ◽  
Polycyclic Aromatic

Abstract Due to their wide distribution and availability, plant leaves can be considered interesting candidates as biomonitoring substrates for the evaluation of atmospheric pollution. In addition, some species can also retain historical information, for example, related to environmental pollution, due to their leaf class age. In this study, the content of polycyclic aromatic hydrocarbons (PAHs) in Abies holophylla and Pinus tabuliformis needle samples in function of their class age has been investigated to obtain information regarding the degradation constant for each PAH under investigation (α values ranging from 0.173 to 1.870) and to evaluate the possibility to correlate the presence of PAHs in needles with some important pollution environmental factors. Considering air pollutant variables registered in Jilin Province, significant correlations (at 95% confidence level) have been found between coal consumption per year and anthracene contents in needles, while fluorene, phenanthrene, and anthracene resulted correlated with coal consumption. Furthermore, it has been demonstrated that the total PAH concentration in needles, for both species, increased with their age (from 804 to 3604 ng g− 1 dry weight), showing a general tendency to accumulate these substances through years. PAH degradation rates increased instead with molecular complexity. This study could be considered a first trial to obtain historical environmental information by pine needles biomonitoring.

Application of Immobilized Laccase on Polyurethane Foam for Ex-Situ Polycyclic Aromatic Hydrocarbons Bioremediation

2021 ◽  
Author(s):  
Brayam Luiz Batista Perini ◽  
Naionara Ariete Daronch ◽  
Rodrigo Luiz Bitencourt ◽  
Andréa Lima dos Santos Schneider ◽  
Cristiano José de Andrade ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Polyurethane Foam ◽  
Aromatic Hydrocarbons ◽  
Ex Situ ◽  
Immobilized Laccase ◽  
Polycyclic Aromatic

The Effect of pH Value on the Polycyclic Aromatic Hydrocarbons Degradation in Sludge during Biological Aerobic Fermentation Process

2013 ◽  
Vol 664 ◽  
pp. 72-76 ◽  
Author(s):  
Jie Yu
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Fermentation Process ◽  
Ph Value ◽  
Test Device ◽  
Aerobic Fermentation ◽  
Polycyclic Aromatic ◽  
Negative Effect ◽  
Ph Conditions ◽  
Degradation Effect

The degradation of polycyclic aromatic hydrocarbons in sludge in aerobic fermentation process is affected by many factors, and the pile body pH value is one of the important factors. Through the test of mixing lime pile body regulating pH value (7.7, 8.2, 9), the polycyclic aromatic hydrocarbon degradation effect was researched under the different pH conditions and the same of the other aerobic fermentation conditions. The results showed that, pH=7.7 test device (not mixing lime ) on PAHs degradation effect was best, worst was the pH=8.2 test device, the sludge degradation of the polycyclic aromatic hydrocarbons effect is negative effect.

scholarly journals Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Meng Ma ◽  
Li Zheng ◽  
Xiaofei Yin ◽  
Wei Gao ◽  
Bin Han ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Deep Sea ◽  
Petroleum Hydrocarbons ◽  
Enrichment Culture ◽  
Degradation Efficiency ◽  
Gravimetric Analysis ◽  
The South ◽  
Enrichment Cultures ◽  
Polycyclic Aromatic

AbstractIn this study, sediments were collected from two different sites in the deep-sea hydrothermal region of the South Atlantic Ocean. Two microbial enrichment cultures (H7S and H11S), which were enriched from the sediments collected at two sample sites, could effectively degrade petroleum hydrocarbons. The bacterial diversity was analyzed by high-throughput sequencing method. The petroleum degradation ability were evaluated by gas chromatography–mass spectrometry and gravimetric analysis. We found that the dominant oil-degrading bacteria of enrichment cultures from the deep-sea hydrothermal area belonged to the genera Pseudomonas, Nitratireductor, Acinetobacter, and Brevundimonas. After a 14-day degradation experiment, the enrichment culture H11S, which was obtained near a hydrothermal vent, exhibited a higher degradation efficiency for alkanes (95%) and polycyclic aromatic hydrocarbons (88%) than the enrichment culture H7S. Interestingly, pristane and phytane as biomarkers were degraded up to 90% and 91% respectively by the enrichment culture H11S, and six culturable oil-degrading bacterial strains were isolated. Acinetobacter junii strain H11S-25, Nitratireductor sp. strain H11S-31 and Pseudomonas sp. strain H11S-28 were used at a density ratio of 95:4:1 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.

Effect of UV Intensity and Wavelengh on Photocatalytic Degradation of Polycyclic Aromatic Hydrocarbons

2011 ◽  
Vol 233-235 ◽  
pp. 466-469
Author(s):  
Li Hong Zhang ◽  
Cheng Bin Xu ◽  
Zhong Lin Chen ◽  
Xue Mei Li
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Photocatalytic Degradation ◽  
Light Source ◽  
Uv Irradiation ◽  
Aromatic Hydrocarbons ◽  
Uv Light ◽  
Irradiation Intensity ◽  
Degradation Rates ◽  
Polycyclic Aromatic

The photocatalytic degradation of pyrene (PYRE) and benzo[a]pyrene (BaP) on soil surfaces in the presence of Fe2O3 using ultraviolet (UV) light source was investigated in a photo chamber, in which the temperature was maintained 30 . The effects of UV irradiation intensity and UV wavelength on the degradation performance of polycyclic aromatic hydrocarbons (PAHs) were studied. The results show that with the intensity of UV irradiation being enhanced, the photocatalytic degradation rates of PAHs were increased. Photocatalytic degradation rates of PYRE and BaP on soil surfaces with 5% Fe2O3 were different at UV irradiation wavelengths of 254, 310 and 365 nm, respectively.

scholarly journals Response of Root Exudates of Bruguiera gymnorrhiza (L.) to Exposure of Polycyclic Aromatic Hydrocarbons

2022 ◽  
Vol 9 ◽  
Author(s):  
Beibei Liu ◽  
Lin Wu ◽  
Pan Pan ◽  
Ruilong Li ◽  
Bigui Lin
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Organic Acids ◽  
Root Exudates ◽  
Aromatic Hydrocarbons ◽  
Bruguiera Gymnorrhiza ◽  
Total Sugars ◽  
Mangrove Root ◽  
Degradation Rates ◽  
Hydroponic Cultivation ◽  
Polycyclic Aromatic

Root exudates play a pivotal role in the behaviors of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments, but the knowledge of how mangrove root exudates response to PAHs pollutants is limited. This study examined the root exudates of Bruguiera gymnorrhiza (L.) (B. gymnorrhiza) under exposure in phenanthrene, pyrene, and benzo[a]pyrene solution through a 45 days hydroponic cultivation. The results showed that the root exudates of B. gymnorrhiza were mainly hydrocarbon compounds. Tartaric acid was the dominant low molecular weight organic acids (LMWOAs) in root exudates. Under PAHs stress, the proportion of hydrocarbon compounds in root exudates decreased, while the proportion of amide compounds increased. At the first 15 days exposure, the amounts of dissolved organic carbon, soluble total sugars, total organic acids and LWMOAs all increased and reached the maximum values, subsequently, the amounts of root exudates had dropped. The degradation rates of PAHs followed the sequence of phenanthrene > pyrene > benzo [a] pyrene, and the presence of root exudates can significantly enhance the degradation of PAHs. The results illustrated that PAHs stress can significantly change the concentrations and species of root exudates. This study provides the scientific reference for understanding the ability of B. gymnorrhiza response to PAHs stress.

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