scholarly journals Evaluation of large intestinal mucosa regeneration in ulcerative colitis using linear measurements

2005 ◽  
Vol 58 (1-2) ◽  
pp. 11-18 ◽  
Author(s):  
Matilda Djolai
Keyword(s):  
Ulcerative Colitis ◽  
Intestinal Mucosa ◽  
Unit Length ◽  
Active Phase ◽  
Linear Measurements ◽  
Crypt Epithelium ◽  
Chronic Inflammatory Condition ◽  
Everyday Work ◽  
Remission Phase

Ulcerative colitis is a chronic inflammatory condition characterized by three phases: active, regression and remission phase. The active phase is followed by atrophy of the large intestinal mucosa. Although its evaluation is sometimes difficult, quantification of ceratin mucosal parameters can be used as an accessory method. The aim of the study was to determine the parameters of linear micrometry in order to estimate the regeneration of the large intestinal mucosa in ulcerative colitis, and to evaluate the efficiency of this method in everyday work. The measurements were performed on routine bioptic samples after qualitative histologic analysis and determination of the type and stage of the disease. The measurements were carried out to determine: the number of crypts per unit length, the height of crypt epithelium, diameter of crypts, their lumen and interstices; also, the quotient between the diameters of crypts and interstices was calculated. The analysis of the measured parameters points to presence and degree of regeneration and/or atrophy of mucosa, particularly by following the parameters of crypt epithelium. Linear measurements can be used in estimation of regeneration and atrophy of large intestinal mucosa. .

scholarly journals Expression of Oncogenic Molecules in Pediatric Ulcerative Colitis

Digestion ◽  
2021 ◽  
pp. 1-9
Author(s):  
Nobuyasu Arai ◽  
Takahiro Kudo ◽  
Kazuhide Tokita ◽  
Reiko Kyodo ◽  
Masamichi Sato ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ulcerative Colitis ◽  
Real Time ◽  
Real Time Pcr ◽  
Immunohistochemical Staining ◽  
Active Phase ◽  
Control Groups ◽  
Expression Levels ◽  
Remission Phase ◽  
And Control

<b><i>Introduction:</i></b> Long-term disease duration of ulcerative colitis (UC) is known to increase the risk of developing colorectal cancer in adults; however, this association has not been genetically analyzed in children with UC. Herein, we examined the expression of cancer-related genes in the colonic mucosa of pediatric UC patients and their risk of developing colorectal cancer. <b><i>Methods:</i></b> Microarray analysis of cancer-related gene expression was conducted on rectal mucosa biopsy specimens randomly selected from pediatric cases, including 4 active-phase UC cases, 3 remission-phase UC cases, and 3 irritable bowel syndrome control cases. The subject pool was then expanded to 10 active-phase cases, 10 remission-phase cases, and 10 controls, which were analyzed by real-time polymerase chain reaction (PCR) and immunohistochemical staining. <b><i>Results:</i></b> The microarray results indicated significantly higher expression levels of cancer-related genes <i>PIM2</i> and <i>SPI1</i> in the active group than in the remission and control groups (<i>p</i> &#x3c; 0.05). Real-time PCR confirmed that <i>PIM2</i> and <i>SPI1</i> expression levels were significantly higher, whereas <i>TP53</i> and <i>APC</i> expression levels were significantly lower, in the active-phase group than in the remission and control groups (<i>p</i> &#x3c; 0.05). Immunohistochemical staining for PIM2, SPI1, TP53, and APC proteins supported the real-time PCR results. <b><i>Conclusions:</i></b> Expression levels of previously unreported cancer-related genes in adult UC patients were significantly higher in pediatric UC patients than in controls. Inflammation of the gastrointestinal mucosa increased the expression levels of cancer-related genes even in childhood-onset UC cases, suggesting that chronic inflammation from childhood may increase the risk of colorectal cancer development.

scholarly journals P042 Diagnostic and prognostic value of soluble adhesion molecules in patients with ulcerative colitis

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S152-S152
Author(s):  
G Tarasova ◽  
N Dobaeva ◽  
A Iakovlev ◽  
A Volkov
Keyword(s):  
Ulcerative Colitis ◽  
Adhesion Molecules ◽  
Experimental Studies ◽  
Fold Increase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Moderate Increase ◽  
Soluble Adhesion Molecules ◽  
Markers Of Inflammation ◽  
Remission Phase

Abstract Background Experimental studies have shown that cell adhesion molecules contribute to the constant induction of pro-inflammatory cytokines (interleukin (6, 8, 10), necrosis factor of tumor-α), providing the chronicity of immune-mediated inflammation at ulcerative colitis (UC). Determination of the importance of molecules of integrin adhesion (sVCAM-1) and mucosal adressin (sMAdCAM-1) for evaluating the effectiveness of treatment of patients with UC. Methods 119 patients with UC were examined: 17 (14.3%) with proctitis, 44 (37%) with left-sided localization, 58 (48.7%) with the total form. The comparison group consisted of 20 healthy volunteers. Determination of serum sVCAM and sMAdCAM was carried out by quantitative enzyme-linked immunosorbent assay (ELISA) based on a system for multiplex analysis using flow fluorimetry — Luminex MAGPIX (USA), ICAM-1 kit (Cusabio, USA). The indicators were assessed before the start of the basic therapy course, in accordance with national recommendations, and 12 weeks after its completion. Results In patients with left-sided and total UC, a 3.2 and 4.7-fold increase in sVCAM expression and a 2.8, 3.6-fold increase in sMAdCAM expression was recorded, averaging: 386.6 + 21.2 ng/ml, 623.4 + 11.1 ng/ml; 116.5 + 13.6 ng/ml, 193.8 + 15.2 ng/ml, respectively (p &lt;0.001). In the group with rectal lesions, there was a moderate increase in the expression of adhesion molecules: 157.1 + 11.3 ng/ml, 84.5 + 18.8 ng/ml, respectively (p&lt;0.07). Against the background of a 12-week treatment course, clinical and endoscopic remission was recorded in 84 (70.6%) patients. In the groups of patients with left-sided and total UC in the drug remission phase, there was a decrease in the expression of sVCAM (194.6 + 9.2 ng/ml, 236.7 + 14.1 ng/ml) and sMAdCAM (72.4 + 8.1 ng/ml, 98.2 + 9.7 ng/ml), respectively (p &lt;0.02). In cases with UC activity, increased levels of adhesion molecules persisted. Conclusion The soluble adhesion molecules of sVCAM and sMAdCAM are modern markers of inflammation that can be used for assessing the effectiveness of course therapy of UC.

The determination of ASCA and P-ANCA could be useful in differentiating Crohn's disease from ulcerative colitis

2001 ◽  
Vol 33 ◽  
pp. A118
Author(s):  
P.E. Lecis ◽  
B. Gennan ◽  
G. Bertiato ◽  
C. Papa ◽  
E. Galliani ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease

scholarly journals Innate Immunity Modulation by the IL-33/ST2 System in Intestinal Mucosa

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Marina García-Miguel ◽  
M. Julieta González ◽  
Rodrigo Quera ◽  
Marcela A. Hermoso
Keyword(s):  
Ulcerative Colitis ◽  
Innate Immunity ◽  
Intestinal Mucosa ◽  
Inflammatory Bowel Diseases ◽  
The Body ◽  
Unknown Etiology ◽  
Adequate Food ◽  
Bowel Diseases ◽  
Surface Contact Area ◽  
Inflammatory Bowel

Innate immunity prevents pathogens from entering and spreading within the body. This function is especially important in the gastrointestinal tract and skin, as these organs have a large surface contact area with the outside environment. In the intestine, luminal commensal bacteria are necessary for adequate food digestion and play a crucial role in tolerance to benign antigens. Immune system damage can create an intestinal inflammatory response, leading to chronic disease including inflammatory bowel diseases (IBD). Ulcerative colitis (UC) is an IBD of unknown etiology with increasing worldwide prevalence. In the intestinal mucosa of UC patients, there is an imbalance in the IL-33/ST2 axis, an important modulator of the innate immune response. This paper reviews the role of the IL-33/ST2 system in innate immunity of the intestinal mucosa and its importance in inflammatory bowel diseases, especially ulcerative colitis.

Selective oxidation of propene to acrolein on FeMoTeO catalysts: determination of active phase and enhancement of catalytic activity and stability

2017 ◽  
Vol 7 (20) ◽  
pp. 4629-4639 ◽  
Author(s):  
M. Tonelli ◽  
M. Aouine ◽  
L. Massin ◽  
V. Belliere Baca ◽  
J. M. M. Millet
Keyword(s):  
Catalytic Activity ◽  
Selective Oxidation ◽  
Active Phase ◽  
Propene Oxidation

Multicomponent FeMoTeO catalysts have been synthesized and studied for mild propene oxidation to acrolein.

scholarly journals A NON-DESTRUCTIVE METHOD FOR ESTIMATING LEAF AREA OF Ceiba glaziovii (Kuntze) K. Schum.

FLORESTA ◽  
2019 ◽  
Vol 50 (1) ◽  
pp. 1063
Author(s):  
João Everthon da Silva Ribeiro ◽  
Francisco Romário Andrade Figueiredo ◽  
Ester Dos Santos Coêlho ◽  
Walter Esfrain Pereira ◽  
Manoel Bandeira de Albuquerque
Keyword(s):  
Leaf Area ◽  
Regression Models ◽  
Information Criterion ◽  
Coefficient Of Determination ◽  
Linear Measurements ◽  
Maximum Width ◽  
Bias Index ◽  
Different Shapes ◽  
Non Destructive

The determination of leaf area is of fundamental importance in studies involving ecological and ecophysiological aspects of forest species. The objective of this research was to adjust an equation to determine the leaf area of Ceiba glaziovii as a function of linear measurements of leaves. Six hundred healthy leaf limbs were collected in different matrices, with different shapes and sizes, in the Mata do Pau-Ferro State Park, Areia, Paraíba state, Northeast Brazil. The maximum length (L), maximum width (W), product between length and width (L.W), and leaf area of the leaf limbs were calculated. The regression models used to construct equations were: linear, linear without intercept, quadratic, cubic, power and exponential. The criteria for choosing the best equation were based on the coefficient of determination (R²), Akaike information criterion (AIC), root mean square error (RMSE), Willmott concordance index (d) and BIAS index. All the proposed equations satisfactorily estimate the leaf area of C. glaziovii, due to their high determination coefficients (R² ≥ 0.851). The linear model without intercept, using the product between length and width (L.W), presented the best criteria to estimate the leaf area of the species, using the equation 0.4549*LW.

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