scholarly journals Preparation and testing of solutions for organ perfusion and preservation in transplantation

2008 ◽  
Vol 65 (8) ◽  
pp. 596-600
Author(s):  
Mirjana Antunovic ◽  
Dragana Aleksic

Background/Aim. Histidine-tryptophan-ketoglutarate (HTK) solution is the most frequently used solution for the preservation of abdominal organs by leading transplant centers worldwide. The aim of the study was to investigate whether it is possible to prepare this kind of perfusion solution in the hospital pharmacy. In the hospital pharmacy setting we prepared HTK preservation solution and tested its quality, and in accordance with the obtained results recommended its shelf-life. Methods. A multicomponent HTK solution was prepared following standardized aseptic procedures for sterile products. Components of HTK solution were dissolved in water for injection under aseptic conditions and the obtained solution was sterilized by bacteriological membrane filtration (0,22 ?m). The quality of the produced solution was examined using physical and physico-chemical methods and biological tests. Following pharmacopoeial regulations, we examined clarity, pH value, sterility and performed testing on pyrogens. The content of the amino acids histidine and triptophan was determined by using high pressure liquid chromatography (HPLC) method. The concentrations of chloride ions were investigated by retitration method. Atomic absorption spectrometry and ion-exchange chromatography with conductometric detection were used for determining the level of magnesium and calcium ions and the concentrations of sodium and potassium ions in HTK solution, respectively. Results. It was shown that the quality of the prepared HTK solution complied with the regulation demands. Also, pH value of the solution as a function of storage temperature, remained in the physiological range (pH 7.4). Conclusion. According to the obtained results of physico-chemical and biological testing it can be concluded that in the hospital pharmacy setting with the applied technological procedure, we can produce HTK preservation solution of the required quality. Keeping of HTK preservation solution in a refrigerator at 4?C warrants its shelf-life of six months. .

2011 ◽  
Vol 17 (1) ◽  
pp. 107-115 ◽  
Author(s):  
Nada Babovic ◽  
Dejan Markovic ◽  
Vojkan Dimitrijevic ◽  
Dragan Markovic

This paper shows the results obtained in field analysis performed at the Tamis River, starting from the settlement Jasa Tomic - border between Serbia and Romania to Pancevo - confluence of Tamis into the Danube. The Tamis is a 359 km long river rising in the southern Carpathian Mountains. It flows through the Banat region and flows into the Danube near Pancevo. During the years the water quality of the river has severely deteriorated and badly affected the environment and the river ecosystem. In situ measurements enabled determination of physico-chemical parameters of water quality of the Tamis River on every 400 m of the watercourse, such as: water temperature, pH value, electrical conductivity, contents of dissolved oxygen and oxygen saturation. The main reason of higher pollution of Tamis is seen in connection to DTD hydro system. Sampling was performed at 7 points with regard to color, turbidity, total hardness, alkalinity, concentration of ammonium nitrogen, nitrite nitrogen, nitrate nitrogen, iron, chlorides and sulphates in samples. The aim of the present work was to evaluate water quality in the Tamis River taking into account significant pollution, which originates from settlements, industry and agriculture, and to suggest appropriate preventive measures to further pollution decreasing of the river's water.


Author(s):  
Dorota Zielińska ◽  
Beata Bilska ◽  
Katarzyna Marciniak-Łukasiak ◽  
Anna Łepecka ◽  
Monika Trząskowska ◽  
...  

Food labelled with a “best before” date has a long shelf life. This study aimed to examine the respondents’ knowledge and understanding regarding the labelling on food products, as well as to assess the microbiological, physico–chemical and the sensory quality of selected durable food products on and after the date specified by the manufacturer. Two methods were used—a survey and laboratory tests. It was found that the majority of respondents have difficulty distinguishing and understanding the terms on the label and that a significant proportion of the respondents consume food products after the “best before” date. Laboratory tests of milk, pasta, mayonnaise and jam confirmed the microbiological safety of the products even six months after the “best before” date. Other features (texture, colour and sensory quality) slightly changed after one month for milk and mayonnaise (the colour had become more yellow) and after three months for pasta (its hardness had decreased) and jam (it had become browner). The possibility of extending the “best before” dates of selected durable foods could be considered, which could allow such products to legally be handed over to public benefit organisations, thereby reducing food wastage.


2014 ◽  
Vol 1051 ◽  
pp. 378-382
Author(s):  
Wei Qing Lan ◽  
Jing Xie ◽  
Feng Zhu ◽  
Rui Qi Zhu

The effects of modified atmosphere packaging (MAP: 80%CO2/20%O2) and chitosan (CS: 10.0g/L) on the shelf-life of pomfret (Pampus argenteus) fillets during chilled storage were studied. Quality assessment was based on biochemical (total viable counts (TVC), total volatile base nitrogen (TVB-N), tri-methylamine (TMA-N), K-value, pH value) and sensory analysis indices determination. The results showed that MAP and CS could keep the sensory quality of Pampus argenteus, delay its deteriorating speeds and slow down the increase of K-value effectively. Compared with the control samples, the shelf-life of pomfret samples in MAP and CS stored at 4±1°C were 10, 12d respectively.


Author(s):  
Tiparat TIKAPUNYA

The purpose of this research is to investigate the quality-related physical, chemical, and microbiological changes in Thai cocoa beans during fermentation in 2 types of wooden containers. The results will compose a book of guidelines for good Thai cocoa fermentation in order to educate Thai farmers. Fresh Thai cocoa beans have a low pH value (5.0 - 5.5) compared to those from other countries in general (6.0 - 7.0). However, fermented temperature is able to reach 40 - 45 °C in 6 days, which is a main criteria for finishing cocoa fermentation. The color of fresh cocoa beans changes from white to brown within 2 days; after that, cocoa beans are mixed from the top to the bottom of the containers. Three groups of microorganism are evaluated with 3 different sampling points in wooden containers. The results reveal that yeast is grown quickly in 2 days on the top of containers, and then acetic acid bacteria and lactic acid bacteria are grown by the utilized yeast’s metabolites. These behaviors were found in both of the 2 wooden containers; however, a heap of cocoa beans (200 - 250 kgs) in a wooden box showed better quality of cocoa fermentation than a small volume (40 - 50 kgs) in a wooden tray. HIGHLIGHTS Understanding changes in Thai cocoa beans fermentation based on traditional method Two type of wooden containers applied for comparing all quality-related cocoa fermentation Proper cocoa beans fermentation process composed to a book of guideline for Thai Farmers GRAPHICAL ABSTRACT


Author(s):  
Ravinder Singh ◽  
Kaushik Khamrui

Pinni, a native heat dried sweet produce ready by roasting cereal flours mainly, with added khoa (desiccated milk) and sugar has a shelf-life of a few days under marketplace. In this study, result of preservers viz. i.e., cardamom powder and rosemary on the storage-life of pinni stored at 30±1˚C was investigated. Two packaging techniques viz., conventional cardboard boxes and polystyrene tray were compared for physico-chemical, quality of pinni during storage for 21 days and 28 days respectively at 30±1˚C. During storing, several variations took place in physico-chemical appearances, the changes being faster in control samples packed in both packaging materials as compared to cardamom and rosemary extract added pinni samples. The average tyrosine content for fresh pinni was 9.75 μg/5ml in control, 9.35 μg/5ml in cardamom and 9.45 μg/5ml in rosemary extracted added sample which increased significantly (p<0.05) to 13.87 μg/5ml, 13.59 μg/5ml and 13.23 μg/5ml respectively when packed in cardboard boxes similar results were found when pinni packed in polystyrene tray. There was slight effect of preservers on the excellence of pinni when packed in cardboard boxes, but changes were slower in pinni when packed in polystyrene tray. Pinni packaged in polystyrene tray stored at 30±1˚C, in the presence rosemary extract, was stable for 28 days and have shelf life 14 days more than control sample.


Author(s):  
Н.Н. АЛЕХИНА ◽  
Е.И. ПОНОМАРЕВА

Для обогащения хлебобулочных изделий используют биоактивированное зерно пшеницы, что обусловливает введение дополнительной стадии технологического процесса – биоактивации. Снизить трудоемкость и сократить продолжительность процесса приготовления зернового хлеба из биоактивированной пшеницы, что особенно важно в условиях предприятий малой мощности, позволяет применение технологии глубокой заморозки теста. Однако продолжительная заморозка снижает активность клеток бродильной микрофлоры теста, поэтому необходимо установить оптимальный срок хранения замороженного тестового полуфабриката. Для исследований зерно пшеницы предварительно очищали от сорной и зерновой примеси, мыли, оставляли для набухания в воде на 22–24 ч, проращивали в течение 11–12 ч и измельчали путем пропускания через матрицу с диаметром отверстий 2 мм. Тестовые полуфабрикаты изготавливали по рецептуре для хлеба «Айсбрэд», затем их подвергали шоковой заморозке при температуре –35°С и хранили при –18°С в течение 7, 14, 21 и 28 сут. Перед использованием тесто размораживали, полуфабрикат выбраживал, из него формовали тестовые заготовки массой 0,07 кг, которые после расстойки выпекали. Контролем служило тесто без заморозки, приготовленное по рецептуре для хлеба «Колосок». Через 20 ч после выпечки оценивали физико-химические и органолептические показатели изделий. Методом микроскопирования выявлено, что с увеличением продолжительности хранения в замороженных тестовых полуфабрикатах повышается содержание мертвых клеток дрожжей. Наименьшее количество их наблюдалось в образце теста без заморозки – 12,2%, что на 29,0% меньше, чем в образце замороженного теста при хранении 28 сут. Установлено, что при хранении замороженного теста в течение 7, 21 и 28 сут содержание восстановленного глутатиона в нем соответственно на 1,6; 7,4 и 7,8% выше, чем в тесте без заморозки. Количество выделенного диоксида углерода через 120 мин брожения в образцах замороженного теста, хранившихся 7, 14, 21 и 28 сут, было меньше в 1,4; 1,5; 2,5 и 2,8 раза соответственно, чем в контрольном образце. По показателям качества изделия, выпеченные из теста после заморозки и без нее, существенно не отличались. На основании результатов исследований рекомендовано хранить замороженный тестовый полуфабрикат для приготовления зернового хлеба при температуре –18°С не более чем 21 сут. For the enrichment of bakery products, bioactivated wheat grain is used, which leads to the introduction of an additional stage of the technological process – bioactivation. The use of deep-freezing technology allows you to reduce the labor intensity and reduce the duration of the process of preparing grain bread from bioactivated wheat, which is especially important in the conditions of small-scale enterprises. However prolonged freezing reduces the activity of the cells of the fermentation microflora of the dough, so it is necessary to establish the optimal shelf life of the frozen dough semi-finished product. For research, wheat grain was previously cleaned from weed and grain impurities, washed, left to swell in water for 22–24 hours, germinated for 11–12 hours and ground by passing through a matrix with a hole diameter of 2 mm. The dough pieces were made according to the recipe for «Aysbred» bread, then they were subjected to shock freezing at a temperature of –35°C and stored at –18°C for 7, 14, 21 and 28 days. Before use, the dough was thawed, the semi-finished product was fermented, and dough pieces weighing 0,07 kg were formed from it, which were baked after proofing. The control was the dough without freezing, prepared according to the recipe for bread «Kolosok». After 20 hours after baking, the physico-chemical and organoleptic parameters of the baked bread were evaluated. Microscopy revealed that with an increase in the shelf life of frozen semi-finished products, the content of dead yeast cells increases. Their lowest number was observed in the sample of the test without freezing – 12,2%, which is by 29,0% less than in the sample of the frozen test, stored for 28 days. It was found that in the frozen dough samples stored for 7, 21 and 28 days, the content of reduced glutathione was by 1,6; 7,4 and 7,8% higher respectively, than in the control sample. After 120 min of fermentation, the amount of carbon dioxide released in the frozen dough samples stored for 7, 14, 21 and 28 days was in 1,4; 1,5; 2,5 and 2,8 times less respectively, than in the test without freezing. In terms of quality, the products baked from the dough after freezing and without it did not differ significantly. Based on the research results, it is recommended to store the frozen test semi-finished product for the preparation of grain bread at a temperature of –18°C for no more than 21 days.


2020 ◽  
Vol 141 ◽  
pp. 02002
Author(s):  
Phanida Renumarn ◽  
Natthaya Choosuk

In the present study, influence of two types packaging (inflated polypropylene (IPP) and laminated aluminium foil (ALU)) and storage conditions (with/without 1 g of silica desiccant packets (SDPs)) on quality and shelf life of chewy santol candies were studied. After storage at 25 degrees Celsius for 30 days, it was found that the combination of ALU with SDPs presented the best treatment to maintain the quality of colour, water activity (aw), moisture content, total acidity, pH value, sensory evaluation i.e. (colour, odour, flavour and overall acceptance). Shelf-life prediction by using accelerated Q10 method based on moisture factors as an indicator of deterioration of the samples during storage. The samples were incubated at 25, 35 and 45°C and sampled every 5 days for estimated on physical, chemical quality and microbiological change. The predicted shelf life of chewy santol candies at 25°C of IPP and ALU packaging with SDPs using Q10 method were 25 and 27 days, respectively. However, the IPP and ALU packaging without SDPs, the products can be stored less than 25 days. SDPs provides a cheaper and easy method to keeping quality of the chewy santol candies. Therefore, the products with SDPs packaging can maintain the product quality during storage and has an acceptable quality to consumers.


INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 36-41
Author(s):  
Anu Ruhila ◽  
◽  
Pramod R. Yadav ◽  
Galib R ◽  
Pradeep K. Prajapati

Bhallataka Taila (Oil) is extracted with the help of Patala Yantra. It is one of the important formulations in Ayurveda pharmaceutics. Shelf life for various dosage forms are described in Ayurveda classics. In the current scenario, the amendment of Rule No. 161-B of the Drugs and Cosmetic Act 1940, specifies the maximum shelf life of Ayurvedic formulations. However, there is a lack of shelf life data for individual for-mulations. Thus, the present study is aimed to evaluate the shelf life of Bhallataka Taila (Oil). Bhallataka Taila (Oil) was extracted following classical guidelines. The sample was subjected to an accelerated stability study maintaining the temperature and humidity 40 +- 2 0C and 75 +- 5 %, respectively. Physico-chemical parameters were analysed at the initiation of the study and at an interval of 1, 3 and 6 months to check the degradation levels in the formulation. Tests for microbial limit and heavy metals were also conducted for the safety and quality of the product. On extrapolating the observations, the shelf life for Bhallataka Taila (Oil) was found to be 4.89 years, which exceeds the shelf life mentioned for Taila (Oil) in official gazettes of the Government of India. This oil preparation does not incorporate any liquid media such as Swarasa (Juice), Kwatha (decoction), etc. as required in any Taila Preparation, resulting in low degradation rate. The oil is rich in PUFA (polyunsaturated fatty acid) content and tocopherols, which may contribute to the stability of the oil.


2013 ◽  
Vol 781-784 ◽  
pp. 1582-1588 ◽  
Author(s):  
Jian Bing Shi ◽  
Jing Xie ◽  
Zhi Li Gao ◽  
Liu Li ◽  
Qing Xiong ◽  
...  

The purpose of this study was to evaluate the impact of different concentrations of tea polyphenols (A1: 0.1%(w/v), A2: 0.5%, A3: 1%), lysozyme (B1: 0.01%, B2: 0.05%, B3: 0.08%) and chitosan (C1: 0.5%, C2: 1%, C3: 2%, C4: 3%) on the quality of pomfret during the storage in order to improve the quality of pomfret fresh preservation. The sensory evaluation, aerobic plate count (APC), trimethylamine (TMA), total volatile basis nitrogen (TVB-N),Kvalue and pH value with different treatments were determined. The results showed that the shelf-life of the control group was 4 days under the cold storage, and the effect of fresh preservation was better with the increasing of the concentration of tea polyphenols, especially the APC could be significantly lower under the treatment groups of A2 and A3 than that under the control group from the 2nd day (P<0.05), the shelf-life was increased by 2 to 3 days. The treatments of B1 and B2 could restrain the increasing of gram-positive bacteria and reduced the contain of TVB-N, TMA-N andKvalue significantly (P<0.05), the shelf-life was increased by 1 to 2 days. The preservation effect of the treatment of C3 was better than those of control group and other groups with chitosan. The APC, TMA-N, TVB-N andKvalue could be significantly lower than those of the control group from the 2nd day, so the shelf-life was increased by 3 days. All of tea polyphenols and chitosan could improve the quality of aquaculture and prolong the shelf-life of pomfret by restraining the increasing of APC and the enzyme activity. The results will offer theory reference to the preservation of pomfrets and the application of bio-preservative.


Sign in / Sign up

Export Citation Format

Share Document