scholarly journals Histopathological evaluation of bone regeneration using human resorbable demineralized membrane

2010 ◽  
Vol 67 (6) ◽  
pp. 480-486
Author(s):  
Zoran Tatic ◽  
Novak Stamatovic ◽  
Marija Bubalo ◽  
Snezana Jancic ◽  
Alek Racic ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Connective Tissue ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defects ◽  
Histopathological Analysis ◽  
Bony Defect ◽  
Histopathological Evaluation ◽  
Bony Healing ◽  
The Right

Background/Aim. Filling a bone defect with bone substitution materials is a therapy of choice, but the infiltration of connective tissue from the mucoperiostal flap may compromise a healing of bone substitutions with bony wall defects. Application of membrane as a barrier is indicated as a solution to this problem. The aim of this study was to show a pathohistological view of bone regeneration and the significance of human resorbable demineralized membrane (HRDM), 200 ? thick in bone regeneration regarding mandibular defects in an experiment on dogs. Methods. The experiment was performed on six dogs. Bone defects were created in all six dogs on the right side of the mandible after the elevation of the mucoperiostal flap. One defect was filled with human deproteinised bone (HDB), and in between HDB and soft tissue RHDM of 200 ? thick was placed. In the second defect, used as a control one, only HDB without RHDM was placed. Two dogs were sacrificed two months after the surgery, another two dogs four months after the surgery and the last two dogs six months after the surgery. After that, samples of bone tissue were taken for histopathological analysis. Results. In all the six dogs with defects treated with HDB and RHDM the level of bone regeneration was much higher in comparison with the control defects without RHDM. Conclusion. Membrane, as a cover of bony defect, is useful and benefits bone regeneration. Bony defects covered with RHDM show better bony healing despite the fact that bone regeneration was not fully complete for as long as six months after the RHDM implantation.

scholarly journals The impact of thickness of resorbable membrane of human origin on the ossification of bone defects: A pathohistologic study

2012 ◽  
Vol 69 (12) ◽  
pp. 1076-1083 ◽  
Author(s):  
Marija Bubalo ◽  
Zoran Lazic ◽  
Smiljana Matic ◽  
Zoran Tatic ◽  
Radomir Milovic ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Blood Clot ◽  
Bone Defects ◽  
Histopathological Analysis ◽  
Bony Defect ◽  
The Third ◽  
Barrier Membrane ◽  
Wide Range ◽  
The Impact

Background/Aim. A wide range of resorbable and nonresorbable membranes have been investigated over the last two decades. The barrier membrane protects the defect from ingrowth of soft tissue cells and allows bone progenitor cells to develop bone within a blood clot that is formed beneath the barrier membrane. The membranes are applied to reconstruct small bony defect prior to implantation, to cover dehiscences and fenestrations around dental implants. The aim of this study was to evaluate the influence of human resorbable demineralized membrane (RHDM) thickness on bone regeneration. Methods. The experiment, approved by Ethical Committee, was performed on 6 dogs and conducted into three phases. Bone defects were created in all the 6 dogs on the left side of the mandible, 8 weeks after extraction of second, third and fourth premolars. One defect was covered with RHDM 100 ? thick, one with RHDM 200 ? thick, and the third defect left empty (control defect). The histopathological analysis was done 2, 4 and 6 months after the surgery. In the third phase samples of bone tissue were taken and subjected to histopathological analysis. Results. In all the 6 dogs the defects treated with RHDM 200 ? thick showed higher level of bone regeneration in comparison with the defect treated with RHDM 100 ? thick and especially with empty defect. Conclusion. Our results demonstrated that the thicker membrane showed the least soft tissue ingrowths and promoted better bone formation at 6 months compared with a thinner one.

Guided Bone Regeneration in Calvarial Bone Defects Using Polytetrafluoroethylene Membranes

1995 ◽  
Vol 32 (4) ◽  
pp. 311-317 ◽  
Author(s):  
Carles Bosch ◽  
Birte Melsen ◽  
Karin Vargervik
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Dura Mater ◽  
Bone Defects ◽  
Guided Bone Regeneration ◽  
Parietal Bone ◽  
Bony Defect ◽  
Calvarial Bone ◽  
Double Membrane ◽  
Single Membrane

Guided bone regeneration is defined as controlled stimulation of new bone formation in a bony defect, either by osteogenesis, osteoinduction, or osteoconduction, re-establishing both structural and functional characteristics. Bony defects may be found as a result of congenital anomalies, trauma, neoplasms, or infectious conditions. Such conditions are often associated with severe functional and esthetic problems. Corrective treatment is often complicated by limitations in tissue adaptations. The aim of the investigation was to compare histologically the amount of bone formed in an experimentally created parietal bone defect protected with one or two polytetrafluoroethylene membranes with a contralateral control defect. A bony defect was created bilaterally in the parietal bone lateral to the sagittal suture in 29 6-month-old male Wistar rats. The animals were divided into two groups: (1) In the double membrane group (n=9), the left experimental bone defect was protected by an outer polytetrafluoroethylene membrane under the periosteum and parietal muscles and an inner membrane between the dura mater and the parietal bone. (2) In the single membrane group (n=20), only the outer membrane was placed. The right defect was not covered with any membrane and served as control. The animals were killed after 30 days. None of the control defects demonstrated complete or partial bone regeneration. In the single membrane group, the experimental site did not regenerate in 15 animals, partially in four, and completely in one. In the double membrane group, six of the experimental defects had complete closure with bone, two had partial closure, and one no closure. The use of two membranes protecting the bone edges of the parietal defect from the overlying tissues and underlying brain enhanced bone regeneration in experimental calvarial bone defects. The biologic role of the dura mater may not be of critical importance in new bone regeneration in these calvarial bone defects.

scholarly journals Sialolipoma of the sublingual salivary gland in a dog – first report

2019 ◽  
Vol 56 (1) ◽  
pp. e149060
Author(s):  
Andressa Gianotti Campos ◽  
Geni Patricio ◽  
Patrícia Ferreira de Castro ◽  
Luciane Kanayama ◽  
Alessandra Loureiro Morales dos Santos ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Soft Tissue ◽  
Fibrous Capsule ◽  
Resected Specimen ◽  
Histopathological Analysis ◽  
First Report ◽  
First Case ◽  
Large Numbers ◽  
Soft Tissue Swelling ◽  
The Right

This report describes a 14-year-old Pit Bull dog presenting with a soft tissue swelling of 3-month progression in the right sublingual region. Histopathological analysis of the surgically resected specimen revealed large numbers of mature adipocytes and islets consisting of mucin-containing atrophic acini and dilated ducts surrounded by a thin fibrous capsule. Findings were consistent with sialolipoma of the sublingual salivary gland. To the authors’ knowledge, this is the first case of sialolipoma affecting the sublingual salivary gland in dogs.

scholarly journals Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

2017 ◽  
Vol 8 ◽  
pp. 204173141771207 ◽  
Author(s):  
Mathieu Maisani ◽  
Daniele Pezzoli ◽  
Olivier Chassande ◽  
Diego Mantovani
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Issue ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Differentiation Potential ◽  
Promising Alternative

Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue. To achieve this goal, cells must survive, remain in the defect site, eventually proliferate, and differentiate into mature osteoblasts. A critical issue for these engrafted cells is to be fed by oxygen and nutrients: the transient absence of a vascular network upon implantation is a major challenge for cells to survive in the site of implantation, and different strategies can be followed to promote cell survival under poor oxygen and nutrient supply and to promote rapid vascularization of the defect area. These strategies involve the use of scaffolds designed to create the appropriate micro-environment for cells to survive, proliferate, and differentiate in vitro and in vivo. Hydrogels are an eclectic class of materials that can be easily cellularized and provide effective, minimally invasive approaches to fill bone defects and favor bone tissue regeneration. Furthermore, by playing on their composition and processing, it is possible to obtain biocompatible systems with adequate chemical, biological, and mechanical properties. However, only a good combination of scaffold and cells, possibly with the aid of incorporated growth factors, can lead to successful results in bone regeneration. This review presents the strategies used to design cellularized hydrogel-based systems for bone regeneration, identifying the key parameters of the many different micro-environments created within hydrogels.

scholarly journals Surface Functionalization of Poly(l-lactide-co-glycolide) Membranes with RGD-Grafted Poly(2-oxazoline) for Periodontal Tissue Engineering

2022 ◽  
Vol 13 (1) ◽  
pp. 4
Author(s):  
Anna M. Tryba ◽  
Małgorzata Krok-Borkowicz ◽  
Michał Kula ◽  
Natalia Piergies ◽  
Mateusz Marzec ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Methylene Chloride ◽  
Membrane Surface ◽  
Bone Cell ◽  
Bone Tissue Regeneration ◽  
Bony Defect ◽  
Surface Modified

Bone tissue defects resulting from periodontal disease are often treated using guided tissue regeneration (GTR). The barrier membranes utilized here should prevent soft tissue infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(l-lactide-co-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For a novel method of membrane manufacture, the RGD motifs were coupled with the non-ionic amphiphilic polymer poly(2-oxazoline) (POx). The RGD-containing membranes were then prepared by solvent casting of PLGA, POx coupled with RGD (POx_RGD), and poly(ethylene glycol) (PEG) solution in methylene chloride (DCM), followed by DCM evaporation and PEG leaching. Successful coupling of RGD to POx was confirmed spectroscopically by Raman, Fourier transform infrared in attenuated reflection mode (FTIR-ATR), and X-ray photoelectron (XPS) spectroscopy, while successful immobilization of POx_RGD on the membrane surface was confirmed by XPS and FTIR-ATR. The resulting membranes had an asymmetric microstructure, as shown by scanning electron microscopy (SEM), where the glass-cured surface was more porous and had a higher surface area then the air-cured surface. The higher porosity should support bone tissue regeneration, while the air-cured side is more suited to preventing soft tissue infiltration. The behavior of osteoblast-like cells on PLGA membranes modified with POx_RGD was compared to cell behavior on PLGA foil, non-modified PLGA membranes, or PLGA membranes modified only with POx. For this, MG-63 cells were cultured for 4, 24, and 96 h on the membranes and analyzed by metabolic activity tests, live/dead staining, and fluorescent staining of actin fibers. The results showed bone cell adhesion, proliferation, and viability to be the highest on membranes modified with POx_RGD, making them possible candidates for GTR applications in periodontology and in bone tissue engineering.

scholarly journals Dynamics of reparative histogenesis of bone tissue in presence of some osteoplastic materials in vitro

2020 ◽  
Vol 4 (34) ◽  
pp. 46-50
Author(s):  
S. Yu. Ivanov ◽  
A. V. Volkov ◽  
D. A. De
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Matrix ◽  
Three Dimensional ◽  
Bone Defects ◽  
Control Group ◽  
Bovine Bone ◽  
Reparative Osteogenesis ◽  
The Impact

Currently, to solve the bone deficiency problem in the maxillofacial region, osteoplastic materials based on allogeneic and xenogenic collagen bone matrix are used, both in pure and in activated forms, by adding growth factors. It is impossible to determine the effectiveness and mechanisms of the osteoplastic materials effect on bone regeneration without a comprehensive study, including not only histological, but also morphometric studies of the structural components and cellular reactions in the impact area. Such studies provide reliable and objective information on the main processes taking place in bone regeneration.Purpose. To determine the spatial distribution of reparative osteogenesis in the presence of some osteoplastic materials in vitro.Materials and methods. Svetlogorsk breed pigs were used as a biomodel. Depending on the osteoplastic preparations used, the animals were divided into four groups of the two in each: 1st — a preparation based on a natural bovine bone graft was injected into bone defects. 2nd — a preparation based on collagenized porcine transplant was injected into bone defects. 3rd — a preparation consisting of 60 % hydroxyapatite (HA) and 40 % beta-tri-calcium phosphate; 4th — control group — the bone defect healed under a blood clot. Animals were removed from the experiment on the 45th day. We examined sections with a thickness of 20 μm using the method of light and fluorescence microscopy.Results. The results indicate different dynamics of the reparative osteogenesis in the presence of osteoplastic materials of different classes. In group 1, the filling of the defect with newly formed bone tissue is not uniform; in group 2, the filling of the defect with newly formed bone tissue is uniform; in group 3 the filling of the defect with non-formed bone tissue is uneven due to the pronounced hyperostosis; in the control group, the filling of the defect with newly formed bone tissue is not happening.Conclusion. Stimulation, the dynamics of reparative osteogenesis and the three-dimensional organization of bone regenerate depend on the osteoplastic material class, which requires further study of the dynamics and three-dimensional organization of bone regenerate to select the optimal bone-replacing agent.

Guided Bone Regeneration (GBR) on Healing Bone Defects: A Histological Study in Rabbits

2004 ◽  
Vol 5 (2) ◽  
pp. 114-123 ◽  
Author(s):  
Muzaffer Asian ◽  
Göksel Şimşek ◽  
Ertunҫ Dayi
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Optical Microscopy ◽  
Bone Healing ◽  
Histological Study ◽  
Bone Defects ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Healing Bone ◽  
The Right

Abstract In this study, the effects of guided bone regeneration (GBR) on the healing of bone defects were evaluated. Resorbable membranes were placed in experimentally formed cavities in the right posterior tibia of 30 rabbits. Decalcified histological sections were evaluated using optical microscopy at 10, 20, and 30 days after GBR. Osteocondrial bone union, active bone formation and spongiosal bone formation values of the GBR group are higher than the control group. It was found that GBR technique had a positive and accelerating influence in all phases of bone healing. Citation Aslan M, Şimşek G, Dayi E. Guided Bone Regeneration (GBR) on Healing Bone Defects: A Histological Study in Rabbits. J Contemp Dent Pract 2004 May;(5)2:114-123.

scholarly journals Frequency of Various Dermatoses Requiring Histopathological Evaluation For Definite Diagnosis; A Retrospective Analysis

Esculapio ◽  
2020 ◽  
Vol 16 (03, july 2020-Septmber 2020) ◽  
Author(s):  
Hira Tariq ◽  
Rabia Mukhtar ◽  
Abeer Fahad ◽  
Tariq Rashid ◽  
Ambereen Anwar ◽  
...  
Keyword(s):  
Connective Tissue ◽  
High Frequency ◽  
Connective Tissue Diseases ◽  
Histopathological Analysis ◽  
Definite Diagnosis ◽  
Drug Reactions ◽  
Histopathological Evaluation ◽  
Dermatological Disorders ◽  
Chronic Ulcers ◽  
Granulomatous Disorders

Objective: To assess the frequency of various dermatological disorders those require histopathological analysis for definite diagnosis. Methods: We included 669 patients of either gender and all ages in this retrospective study, carried out in the Department of Dermatology, Jinnah Hospital, Lahore for a duration of 2 years. Their clinical data and histopathological reports were analyzed. The dermatoses were categorized into (a) papulosquamous disorders, (b) bullous disorders, (c) eczemas, (d) neoplasia, (e) granulomatous disorders, (f) connective tissue diseases, (g) drug reactions, (h) vasculitides, (i) chronic ulcers and (j) miscellaneous disorders. Results: The frequency of various dermatoses noted were as follows: papulosquamous disorders 28.25%, bullous disorders 11.5%, granulomatous disorders 11.5%, connective tissue diseases 10.61%, miscellaneous disorders 10%, eczemas 9.4%, chronic ulcers 7.17 neoplasia 6.8%, vasculitides 2.84% and drug reactions were 1.79% of total biopsies. Conclusion: The frequency of different diagnostic groups was unique in some respects and confirmed to other studies in others. The significantly high frequency of papulosquamous disorders highlighted the importance of these disorders. Key words: Skin biopsy, papulosquamous disorders, bullous disorders, eczemas, neoplasia, granulomatous disorders, connective tissue diseases, drug reactions, vasculitides, chronic ulcers.

scholarly journals 3D-printed HA15-loaded β-Tricalcium Phosphate/Poly (Lactic-co-glycolic acid) Bone Tissue Scaffold Promotes Bone Regeneration in Rabbit Radial Defects

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Chuanchuan Zheng ◽  
Shokouh Attarilar ◽  
Kai Li ◽  
Chong Wang ◽  
Jia Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Glycolic Acid ◽  
Bone Conduction ◽  
Biomechanical Properties ◽  
Bone Defects ◽  
Tissue Scaffold ◽  
3D Printed

In this study, a β-tricalcium phosphate (β-TCP)/poly (lactic-co-glycolic acid) (PLGA) bone tissue scaffold was loaded with osteogenesis-promoting drug HA15 and constructed by three-dimensional (3D) printing technology. This drug delivery system with favorable biomechanical properties, bone conduction function, and local release of osteogenic drugs could provide the basis for the treatment of bone defects. The biomechanical properties of the scaffold were investigated by compressive testing, showing comparable biomechanical properties with cancellous bone tissue. Furthermore, the microstructure, pore morphology, and condition were studied. Moreover, the drug release concentration, the effect of anti-tuberculosis drugs in vitro and in rabbit radial defects, and the ability of the scaffold to repair the defects were studied. The results show that the scaffold loaded with HA15 can promote cell differentiation into osteoblasts in vitro, targeting HSPA5. The micro-computed tomography scans showed that after 12 weeks of scaffold implantation, the defect of the rabbit radius was repaired and the peripheral blood vessels were regenerated. Thus, HA15 can target HSPA5 to inhibit endoplasmic reticulum stress which finally leads to promotion of osteogenesis, bone regeneration, and angiogenesis in the rabbit bone defect model. Overall, the 3D-printed β-TCP/PLGA-loaded HA15 bone tissue scaffold can be used as a substitute material for the treatment of bone defects because of its unique biomechanical properties and bone conductivity.

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