The impact of thickness of resorbable membrane of human origin on the ossification of bone defects: A pathohistologic study

Background/Aim. A wide range of resorbable and nonresorbable membranes have been investigated over the last two decades. The barrier membrane protects the defect from ingrowth of soft tissue cells and allows bone progenitor cells to develop bone within a blood clot that is formed beneath the barrier membrane. The membranes are applied to reconstruct small bony defect prior to implantation, to cover dehiscences and fenestrations around dental implants. The aim of this study was to evaluate the influence of human resorbable demineralized membrane (RHDM) thickness on bone regeneration. Methods. The experiment, approved by Ethical Committee, was performed on 6 dogs and conducted into three phases. Bone defects were created in all the 6 dogs on the left side of the mandible, 8 weeks after extraction of second, third and fourth premolars. One defect was covered with RHDM 100 ? thick, one with RHDM 200 ? thick, and the third defect left empty (control defect). The histopathological analysis was done 2, 4 and 6 months after the surgery. In the third phase samples of bone tissue were taken and subjected to histopathological analysis. Results. In all the 6 dogs the defects treated with RHDM 200 ? thick showed higher level of bone regeneration in comparison with the defect treated with RHDM 100 ? thick and especially with empty defect. Conclusion. Our results demonstrated that the thicker membrane showed the least soft tissue ingrowths and promoted better bone formation at 6 months compared with a thinner one.

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Histopathological evaluation of bone regeneration using human resorbable demineralized membrane

Vojnosanitetski pregled ◽

10.2298/vsp1006480t ◽

2010 ◽

Vol 67 (6) ◽

pp. 480-486

Author(s):

Zoran Tatic ◽

Novak Stamatovic ◽

Marija Bubalo ◽

Snezana Jancic ◽

Alek Racic ◽

...

Keyword(s):

Soft Tissue ◽

Connective Tissue ◽

Bone Regeneration ◽

Bone Tissue ◽

Bone Defects ◽

Histopathological Analysis ◽

Bony Defect ◽

Histopathological Evaluation ◽

Bony Healing ◽

The Right

Background/Aim. Filling a bone defect with bone substitution materials is a therapy of choice, but the infiltration of connective tissue from the mucoperiostal flap may compromise a healing of bone substitutions with bony wall defects. Application of membrane as a barrier is indicated as a solution to this problem. The aim of this study was to show a pathohistological view of bone regeneration and the significance of human resorbable demineralized membrane (HRDM), 200 ? thick in bone regeneration regarding mandibular defects in an experiment on dogs. Methods. The experiment was performed on six dogs. Bone defects were created in all six dogs on the right side of the mandible after the elevation of the mucoperiostal flap. One defect was filled with human deproteinised bone (HDB), and in between HDB and soft tissue RHDM of 200 ? thick was placed. In the second defect, used as a control one, only HDB without RHDM was placed. Two dogs were sacrificed two months after the surgery, another two dogs four months after the surgery and the last two dogs six months after the surgery. After that, samples of bone tissue were taken for histopathological analysis. Results. In all the six dogs with defects treated with HDB and RHDM the level of bone regeneration was much higher in comparison with the control defects without RHDM. Conclusion. Membrane, as a cover of bony defect, is useful and benefits bone regeneration. Bony defects covered with RHDM show better bony healing despite the fact that bone regeneration was not fully complete for as long as six months after the RHDM implantation.

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Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

Journal of Periodontology ◽

10.1902/jop.2010.090592 ◽

2010 ◽

Vol 81 (6) ◽

pp. 926-933 ◽

Cited By ~ 16

Author(s):

Ronaldo Barcellos de Santana ◽

Carolina Miller Leite de Mattos ◽

Carlos Eduardo Francischone ◽

Thomas Van Dyke

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Guided Bone Regeneration ◽

Tissue Response ◽

Barrier Membrane ◽

Soft Tissue Response

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Guided Bone Regeneration in Calvarial Bone Defects Using Polytetrafluoroethylene Membranes

The Cleft Palate-Craniofacial Journal ◽

10.1597/1545-1569_1995_032_0311_gbricb_2.3.co_2 ◽

1995 ◽

Vol 32 (4) ◽

pp. 311-317 ◽

Cited By ~ 18

Author(s):

Carles Bosch ◽

Birte Melsen ◽

Karin Vargervik

Keyword(s):

Bone Regeneration ◽

Bone Defect ◽

Dura Mater ◽

Bone Defects ◽

Guided Bone Regeneration ◽

Parietal Bone ◽

Bony Defect ◽

Calvarial Bone ◽

Double Membrane ◽

Single Membrane

Guided bone regeneration is defined as controlled stimulation of new bone formation in a bony defect, either by osteogenesis, osteoinduction, or osteoconduction, re-establishing both structural and functional characteristics. Bony defects may be found as a result of congenital anomalies, trauma, neoplasms, or infectious conditions. Such conditions are often associated with severe functional and esthetic problems. Corrective treatment is often complicated by limitations in tissue adaptations. The aim of the investigation was to compare histologically the amount of bone formed in an experimentally created parietal bone defect protected with one or two polytetrafluoroethylene membranes with a contralateral control defect. A bony defect was created bilaterally in the parietal bone lateral to the sagittal suture in 29 6-month-old male Wistar rats. The animals were divided into two groups: (1) In the double membrane group (n=9), the left experimental bone defect was protected by an outer polytetrafluoroethylene membrane under the periosteum and parietal muscles and an inner membrane between the dura mater and the parietal bone. (2) In the single membrane group (n=20), only the outer membrane was placed. The right defect was not covered with any membrane and served as control. The animals were killed after 30 days. None of the control defects demonstrated complete or partial bone regeneration. In the single membrane group, the experimental site did not regenerate in 15 animals, partially in four, and completely in one. In the double membrane group, six of the experimental defects had complete closure with bone, two had partial closure, and one no closure. The use of two membranes protecting the bone edges of the parietal defect from the overlying tissues and underlying brain enhanced bone regeneration in experimental calvarial bone defects. The biologic role of the dura mater may not be of critical importance in new bone regeneration in these calvarial bone defects.

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Guided Bone Regeneration- A Comprehensive Review

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/47728.14714 ◽

2021 ◽

Author(s):

Vineetha Venugopalan ◽

Anegundi Raghavendra Vamsi ◽

Santhosh Shenoy ◽

Karishma Ashok ◽

Biju Thomas

Keyword(s):

Bone Regeneration ◽

Alveolar Bone ◽

Primary Stability ◽

Guided Bone Regeneration ◽

Collagen Membrane ◽

Bony Defect ◽

Graft Material ◽

Bone Augmentation ◽

Wide Range ◽

Space Maintenance

Successful implant treatment requires prosthetically driven placement of an implant, primary stability at placement, and careful living bone management. The resorptive changes of alveolar bone are an inevitable process following tooth loss, periodontal disease or trauma which causes bone defects. This results in various aesthetic and functional complications such as soft tissue recession, infection and inflammation. Various methods have been tried and advocated for augmenting these bone deficiencies. Guided Bone Regeneration (GBR) is a successful modality for bone augmentation with a wide range of indications and helps restore the alveolar ridge dimensions. It utilises the principle of Guided Tissue Regeneration (GTR) for space maintenance within a bony defect. Different types of barrier membranes are being utilised along with various bone grafts in GBR. Thorough knowledge regarding the biology of bone is required before the initiation of any bone augmentation procedure. A combination of Collagen Membrane (CM) and graft material was found successful for GBR. Hence, this review focuses on presentation of best available evidence for various aspects of GBR.

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Implant Site Guided Bone Regeneration and Pontic Site Ridge Preservation: A Case Report

PRILOZI ◽

10.2478/prilozi-2021-0028 ◽

2021 ◽

Vol 42 (2) ◽

pp. 103-108

Author(s):

Darko Veljanovski ◽

Denis Baftijari ◽

Zoran Susak ◽

Aneta Atanasovska Stojanovska

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Soft Tissues ◽

Guided Bone Regeneration ◽

Autogenous Bone ◽

Collagen Membrane ◽

Ridge Preservation ◽

Bovine Bone ◽

Implant Placement ◽

Barrier Membrane

Abstract Guided bone regeneration (GBR) is a therapeutic modality to achieve bone regeneration with the use of barrier membranes. The use of deproteinized bovine bone material (DBBM) for ridge preservation allows the preservation of the edentulous ridge dimensions. Here, we present a case of horizontal GBR using DBBM and a resorbable membrane, with simultaneous implant placement. Simultaneously, ridge preservation of the pontic area, using DBBM within a “socket seal” procedure was performed. Two implants were places at sites 23 and 26 to support a fixed partial denture (FPD). The mesial implant showed exposed buccal threads, which were then covered with autogenous bone particles and small size granules of DBBM. The collagen membrane was stabilized with periosteal mattress suture. Six months postoperatively, CBCT images revealed a stable buccal bone layer at the implant site, indicating a successful GBR procedure. At this point in time, tooth 24 was atraumatically extracted. A ridge preservation was done utilizing DBBM, and a soft tissue graft form the tuber. A ceramic-metal FPD with excellent “white aesthetics” and a harmonic transition zone to the soft tissue was fabricated. At 3 years follow up, the peri-implant bone levels were stable, and the clinical outcomes were excellent. It is concluded that a GBR procedure, utilizing DBBM and a collagen barrier membrane with simultaneous implant placement, as well as ridge preservation using DBBM, are predictable therapeutic methods. However, gentle manipulation of the soft tissues, and wound stability, with tension-free passive closure of the wound margins are prerequisites for a long-term clinical success.

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Does Platelet-Rich Fibrin Decrease Dimensional Changes and Improve Postoperative Comfort in Post-Extraction Sockets? An Overview of Systematic Reviews

Applied Sciences ◽

10.3390/app10175750 ◽

2020 ◽

Vol 10 (17) ◽

pp. 5750 ◽

Cited By ~ 1

Author(s):

Vittorio Moraschini ◽

Carlos Fernando de Almeida Barros Mourão ◽

Rafael Coutinho de Mello Machado ◽

Jhonathan Raphaell Barros Nascimento ◽

Kayvon Javid ◽

...

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Systematic Reviews ◽

Selection Process ◽

Soft Tissue Healing ◽

Socket Preservation ◽

Platelet Rich Fibrin ◽

Dimensional Changes ◽

Tissue Healing ◽

The Impact

This overview aimed to evaluate the methods, quality, and outcomes of systematic reviews (SRs) conducted to investigate the effects of platelet-rich fibrin (PRF) in dental sockets on promoting bone regeneration and soft tissue healing and diminishing the incidence of pain, swelling, trismus, and alveolar osteitis after tooth extraction. An electronic search without date or language restriction was done in PubMed/MEDLINE, Cochrane, and Web of Science until March 2020. Eligibility criteria included SRs that assessed the effect of PRF for human alveolar socket preservation. The quality assessment of the included studies was performed using AMSTAR 2 guidelines. The protocol of this overview was recorded in PROSPERO under the number CRD42018089617. The search and selection process yielded 13 studies published between 2011 and 2018. The analysis of the studies showed inconclusive data for the effect of the PRF and the dimensional changes. There is no definitive evidence for the impact of using PRF alone on bone regeneration in post-extraction sockets. The use of PRF improves soft tissue healing and reduces pain, bleeding, and osteitis in post-extraction sockets.

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Dynamics of reparative histogenesis of bone tissue in presence of some osteoplastic materials in vitro

Medical alphabet ◽

10.33667/2078-5631-2019-4-34(409)-46-50 ◽

2020 ◽

Vol 4 (34) ◽

pp. 46-50

Author(s):

S. Yu. Ivanov ◽

A. V. Volkov ◽

D. A. De

Keyword(s):

Bone Regeneration ◽

Bone Tissue ◽

Bone Matrix ◽

Three Dimensional ◽

Bone Defects ◽

Control Group ◽

Bovine Bone ◽

Reparative Osteogenesis ◽

The Impact

Currently, to solve the bone deficiency problem in the maxillofacial region, osteoplastic materials based on allogeneic and xenogenic collagen bone matrix are used, both in pure and in activated forms, by adding growth factors. It is impossible to determine the effectiveness and mechanisms of the osteoplastic materials effect on bone regeneration without a comprehensive study, including not only histological, but also morphometric studies of the structural components and cellular reactions in the impact area. Such studies provide reliable and objective information on the main processes taking place in bone regeneration.Purpose. To determine the spatial distribution of reparative osteogenesis in the presence of some osteoplastic materials in vitro.Materials and methods. Svetlogorsk breed pigs were used as a biomodel. Depending on the osteoplastic preparations used, the animals were divided into four groups of the two in each: 1st — a preparation based on a natural bovine bone graft was injected into bone defects. 2nd — a preparation based on collagenized porcine transplant was injected into bone defects. 3rd — a preparation consisting of 60 % hydroxyapatite (HA) and 40 % beta-tri-calcium phosphate; 4th — control group — the bone defect healed under a blood clot. Animals were removed from the experiment on the 45th day. We examined sections with a thickness of 20 μm using the method of light and fluorescence microscopy.Results. The results indicate different dynamics of the reparative osteogenesis in the presence of osteoplastic materials of different classes. In group 1, the filling of the defect with newly formed bone tissue is not uniform; in group 2, the filling of the defect with newly formed bone tissue is uniform; in group 3 the filling of the defect with non-formed bone tissue is uneven due to the pronounced hyperostosis; in the control group, the filling of the defect with newly formed bone tissue is not happening.Conclusion. Stimulation, the dynamics of reparative osteogenesis and the three-dimensional organization of bone regenerate depend on the osteoplastic material class, which requires further study of the dynamics and three-dimensional organization of bone regenerate to select the optimal bone-replacing agent.

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Cutting Edge Endogenous Promoting and Exogenous Driven Strategies for Bone Regeneration

International Journal of Molecular Sciences ◽

10.3390/ijms22147724 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7724

Author(s):

Iratxe Macías ◽

Natividad Alcorta-Sevillano ◽

Arantza Infante ◽

Clara I. Rodríguez

Keyword(s):

Bone Loss ◽

Bone Regeneration ◽

Healing Process ◽

Bone Grafts ◽

Bone Defects ◽

Cutting Edge ◽

Host Cells ◽

Age Related ◽

Bone Damage ◽

Wide Range

Bone damage leading to bone loss can arise from a wide range of causes, including those intrinsic to individuals such as infections or diseases with metabolic (diabetes), genetic (osteogenesis imperfecta), and/or age-related (osteoporosis) etiology, or extrinsic ones coming from external insults such as trauma or surgery. Although bone tissue has an intrinsic capacity of self-repair, large bone defects often require anabolic treatments targeting bone formation process and/or bone grafts, aiming to restore bone loss. The current bone surrogates used for clinical purposes are autologous, allogeneic, or xenogeneic bone grafts, which although effective imply a number of limitations: the need to remove bone from another location in the case of autologous transplants and the possibility of an immune rejection when using allogeneic or xenogeneic grafts. To overcome these limitations, cutting edge therapies for skeletal regeneration of bone defects are currently under extensive research with promising results; such as those boosting endogenous bone regeneration, by the stimulation of host cells, or the ones driven exogenously with scaffolds, biomolecules, and mesenchymal stem cells as key players of bone healing process.

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Biocomposites for Orthopedic and Dental Application

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.672.261 ◽

2016 ◽

Vol 672 ◽

pp. 261-275 ◽

Cited By ~ 5

Author(s):

Gabriel Furtos ◽

Laura Silaghi-Dumitrescu ◽

Katarzyna Lewandowska ◽

Alina Sionkowska ◽

Petru Pascuta

Keyword(s):

Mechanical Properties ◽

Bioactive Glass ◽

Bone Regeneration ◽

Recent Work ◽

Glass Ceramics ◽

Bone Defects ◽

Inorganic Filler ◽

Wide Range ◽

Dental Application ◽

Superior Mechanical Properties

The development of polymer and inorganic filler lead to new biocomposite materials with a wide range of applications in orthopedic and dental application. Biomposites possess an excellent biocompatibility, biodegradability and superior mechanical properties. The inclusion of bioactive filler of hydroxyapatite, wollastonite glass-ceramics and bioactive glass could provide bioactivity of biocomposites. This review summarizes the recent work on the development of biocomposites containing biopolymers with different bioactive particles suitable for use in bone defects/bone regeneration and dental application.

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Comparison of Resorbable Membranes for Guided Bone Regeneration of Human and Bovine Origin

Acta veterinaria ◽

10.2478/acve-2014-0045 ◽

2014 ◽

Vol 64 (4) ◽

pp. 477-492

Author(s):

Lazić Zoran ◽

Bubalo Marija ◽

Milović Radomir ◽

Matijević Stevo ◽

Magić Marko ◽

...

Keyword(s):

Soft Tissue ◽

Bone Regeneration ◽

Soft Tissues ◽

Alveolar Bone ◽

Guided Bone Regeneration ◽

Epithelial Migration ◽

Tissue Integration ◽

The Third ◽

Tissue Proliferation ◽

Space Reduction

Abstract The properties of membranes for guided bone regeneration have been described by a number of authors. These involve biocompatibility, appropriate barrier features (mechanical prevention of soft tissue proliferation), tissue integration, immunologic neutrality, preservation of the space for new alveolar bone, and simplicity of application. Such membrane must hold out against the masticatory forces and tissue tension of the fl ap, and prevent the collapse of soft tissues and wound space reduction. The property of integration into the tissue guarantees wound stabilization and inhibits epithelial migration. The aim of this study was to compare and evaluate the infl uence of human resorbable demineralized membrane (RHDM) and bovine resorbable demineralized membrane (RBDM) on bone regeneration. The experiment, approved by the Ethical Committee, was performed on six dogs and conducted into three phases. Bone defects were created in all six dogs on the left side of the mandible, 8 weeks after extraction of the second, third and fourth premolar. One defect was covered with 200 μm thick RHDM, one with 200 μm thick RBDM, and the third defect was left empty (control defect). The pathohistological analysis was done two, four and six months after the surgery. In the third phase samples of bone tissue were taken and subjected to patohistological analysis. In all six dogs the defects treated with RHDM 200μm thick showed a higher level of bone regeneration in comparison with the defect treated with RBDM 200 μm thick and especially with the control defect. Our results demonstrated that the human membrane showed the least soft tissue ingrowth and promoted better bone formation at 6 months compared with a bovine one.

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