A Comparison of the Glutaraldehyde Coagulation Test and Total Serum Protein Estimation as Indicators of Gamma Globulin Levels in Neonatal Ruminants

1988 ◽  
Vol 19 (1/2) ◽  
pp. 14 ◽  
Author(s):  
S. J. Carstairs-Grant ◽  
G. J. Crawshaw ◽  
K. G. Mehren
Keyword(s):  
Serum Protein ◽  
Gamma Globulin ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Test ◽  
Protein Estimation

Automated Procedure for Simultaneously Measuring Total Globulin and Total Protein in Serum

1971 ◽  
Vol 17 (4) ◽  
pp. 301-306 ◽  
Author(s):  
John Savory ◽  
M Geraldine Heintges ◽  
Robert E Sobel
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Continuous Flow ◽  
Gamma Globulin ◽  
Glyoxylic Acid ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Globulin ◽  
Automated Method ◽  
A Minor

Abstract An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acetyltryptophan to human globulin has been derived. Values for total serum globulin obtained by this new automated procedure correlate closely with values obtained by electrophoresis, but do not agree with values obtained by use of procedures involving binding of anionic dye. Recovery of gamma globulin added to serum is essentially quantitative; the day-to-day precision (CV) is 4.31%.

scholarly journals The Metabolism of Serum Proteins in Neonatal Rabbits

1960 ◽  
Vol 43 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Maria P. Deichmiller ◽  
Frank J. Dixon
Keyword(s):  
Serum Protein ◽  
Half Life ◽  
Gamma Globulin ◽  
Serum Proteins ◽  
Growth Period ◽  
Unit Weight ◽  
Total Serum Protein ◽  
Total Serum ◽  
Beta Globulin ◽  
Newborn Animals

1. Incorporation of S35-labeled amino acids into serum proteins has been studied in neonatal and developing rabbits. It was found that, per unit weight, neonatal rabbits synthesized only about 1/36 of the gamma globulin, 1/7 of the beta globulin, ½ of the alpha globulin, and ⅛ of the albumin that an adult synthesized. The growing rabbit developed the ability to synthesize various serum proteins at different times. 2. Plasma volumes and serum protein concentrations were determined at different times during the growth period of the rabbit. Plasma volumes were found to be 1 and ½ times larger in newborn animals than in adults, with a gradual decline to the adult level. The total serum protein concentration at birth was about 60 to 65 per cent of the adult value and gradually increased with growth as the plasma volume decreased. 3. Half-lives of homologous albumin and gamma globulin were studied. The half-life of albumin in neonates was nearly twice as long as the half-life in adults, the latter value being reached at 1 month of age. The half-life of gamma globulin in neonates was more than twice as long as the half-life in adults and reached adult values at 2 to 3 months. 4. Attempts were made to alter serum protein metabolism. Gamma globulin synthesis early in life was augmented with antigen injections.

Serum Protein Values in Premature Infants

PEDIATRICS ◽  
1958 ◽  
Vol 21 (1) ◽  
pp. 26-26
Keyword(s):  
Human Milk ◽  
Serum Protein ◽  
Premature Infants ◽  
Gamma Globulin ◽  
Paper Electrophoresis ◽  
Total Serum Protein ◽  
Total Serum ◽  
Additional Protein ◽  
Protein Components ◽  
Effect Of Feeding

The present investigations were stimulated by an interest in the effect of feeding larger amounts of protein to premature infants than provided by human milk. Twenty-one premature infants between 1500 and 2400 gm in weight and 1 and 56 days of age were studied. The total serum protein and the serum-protein components were determined by paper electrophoresis. During an initial 8 weeks of the experiment the infants received human milk; later 10 of them received additional protein from a skimmed-milk preparation containing 89% casein. During the first 8 weeks the concentration of total protein in the serum decreased from 5.63 to 4.03 gm/100 ml. The decrease was in the albumin fraction (from 3 to 2 gm/100 ml) and the gammaglobulin fraction (from 1 to 0.5 gm/100 ml). The other globulin fractions remained constant. Casein added to the diet resulted in concentrations of total serum protein and serum albumin at higher levels, but the decrease with increasing age was not prevented. The decrease in gamma globulin, concurrent with increasing age, was not prevented by additional casein, on the contrary resulted in a further decrease. The author relates this to other studies in which a lower frequency of infection was found in premature infants fed on human milk than in infants fed on human milk with additional protein.

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

THE EFFECT OF REPEATED HEAT AND COLD EXPOSURE ON SERUM PROTEIN COMPOSITION IN MAN

1967 ◽  
Vol 45 (4) ◽  
pp. 571-575 ◽  
Author(s):  
W. F. Blatt ◽  
J. Kerkay
Keyword(s):  
Serum Protein ◽  
Cold Exposure ◽  
Total Protein ◽  
Protein Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Heat Acclimatization ◽  
Albumin Content ◽  
Protein Serum ◽  
Lipoprotein Distribution

Total protein, serum protein, and lipoprotein electrophoretic distribution and hematocrit values were determined in two groups of men during acclimatization to 6 weeks of cold and 11 days of heat respectively. After 3 weeks of cold exposure total serum protein and albumin content decreased, while the globulin fractions increased; thus, the calculated albumin/globulin ratio was significantly depressed. During the last 2 weeks, these parameters gradually returned to control values. Overall, the protein changes during heat acclimatization were minimal, although the globulins decreased slightly, yielding a small increase in the albumin/globulin ratio. The hematocrit levels were significantly lowered during both environmental exposures, whereas the lipoprotein distribution remained essentially unchanged.

Determination of Total Serum Protein Levels Fed by Hot Smoked Rainbow Trout (Oncorhynchus mykiss) Diets in Rats

2008 ◽  
Vol 3 (4) ◽  
pp. 246-251
Author(s):  
Suleyman Kaleli ◽  
Mustafa Unlusayin ◽  
Sengul Bilgin ◽  
Levent Izci ◽  
Ali Gunlu
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Serum Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Protein Levels ◽  
Rainbow Trout Oncorhynchus Mykiss
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