Automated Procedure for Simultaneously Measuring Total Globulin and Total Protein in Serum

1971 ◽  
Vol 17 (4) ◽  
pp. 301-306 ◽  
Author(s):  
John Savory ◽  
M Geraldine Heintges ◽  
Robert E Sobel
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Continuous Flow ◽  
Gamma Globulin ◽  
Glyoxylic Acid ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Globulin ◽  
Automated Method ◽  
A Minor

Abstract An automated continuous-flow procedure has been developed for simultaneously measuring total serum protein and globulin. The method for total protein is a minor modification of an existing automated method in which the biuret reagent is used. Total globulin is measured by reaction with glyoxylic acid, and standardized with N-acetyltryptophan. An empirical factor relating concentration of N-acetyltryptophan to human globulin has been derived. Values for total serum globulin obtained by this new automated procedure correlate closely with values obtained by electrophoresis, but do not agree with values obtained by use of procedures involving binding of anionic dye. Recovery of gamma globulin added to serum is essentially quantitative; the day-to-day precision (CV) is 4.31%.

THE EFFECT OF REPEATED HEAT AND COLD EXPOSURE ON SERUM PROTEIN COMPOSITION IN MAN

1967 ◽  
Vol 45 (4) ◽  
pp. 571-575 ◽  
Author(s):  
W. F. Blatt ◽  
J. Kerkay
Keyword(s):  
Serum Protein ◽  
Cold Exposure ◽  
Total Protein ◽  
Protein Composition ◽  
Total Serum Protein ◽  
Total Serum ◽  
Heat Acclimatization ◽  
Albumin Content ◽  
Protein Serum ◽  
Lipoprotein Distribution

Total protein, serum protein, and lipoprotein electrophoretic distribution and hematocrit values were determined in two groups of men during acclimatization to 6 weeks of cold and 11 days of heat respectively. After 3 weeks of cold exposure total serum protein and albumin content decreased, while the globulin fractions increased; thus, the calculated albumin/globulin ratio was significantly depressed. During the last 2 weeks, these parameters gradually returned to control values. Overall, the protein changes during heat acclimatization were minimal, although the globulins decreased slightly, yielding a small increase in the albumin/globulin ratio. The hematocrit levels were significantly lowered during both environmental exposures, whereas the lipoprotein distribution remained essentially unchanged.

Serum glycoproteins in growing pups

1962 ◽  
Vol 203 (1) ◽  
pp. 119-121 ◽  
Author(s):  
Otakar V. Sirek ◽  
Anna Sirek
Keyword(s):  
Weight Gain ◽  
Sialic Acid ◽  
Serum Protein ◽  
Total Protein ◽  
Protein Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Albumin Fraction ◽  
The Individual ◽  
Serum Protein Concentration

Total protein-bound hexose, hexosamine, and sialic acid were determined in sera of six littermate mongrel pups at monthly intervals from the 4th day after birth up to the age of 7 months. The concentration of the individual constituents fluctuated considerably from month to month, but the values showed neither a definite trend nor a relationship to weight gain. When the carbohydrate moiety was expressed as percentage of total serum protein concentration, the values were high in newborn pups and diminished after the 1st month of life. This was due to a rise in the concentration of total serum protein, brought about by an increase of the albumin fraction which is low in carbohydrate.

Effect of protein concentration on the determination of digoxin in serum by fluorescence polarization immunoassay.

1984 ◽  
Vol 30 (11) ◽  
pp. 1826-1829 ◽  
Author(s):  
W H Porter ◽  
V M Haver ◽  
B A Bush
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Fluorescence Polarization ◽  
Protein Concentration ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fluorescence Polarization Immunoassay ◽  
Total Protein Concentration ◽  
Protein Pellet

Abstract Determination of digoxin by fluorescence polarization immunoassay (FPIA) with the Abbott "TDx" is significantly influenced by the concentration of total serum protein. Each 10 g/L increase in serum protein results in an 8% decrease in measured digoxin. Studies with [3H]digoxin confirmed that digoxin binds to the protein pellet during the trichloroacetic acid precipitation step before the immunoassay. Serum protein, or equal concentrations of albumin or gamma-globulin, exert an equivalent effect on the apparent digoxin value. Because the total protein concentration of the assay calibrators is low (50 g/L) compared with its reference interval in serum (60-80 g/L), results by FPIA may be expected to be low by an average of 16% (range, 8-24%). Digoxin results by FPIA will be most nearly accurate when the calibrators include a total protein concentration of about 70 g/L. Patients' specimens with abnormally high or low protein content will give falsely high or low results for digoxin.

scholarly journals The Metabolism of Serum Proteins in Neonatal Rabbits

1960 ◽  
Vol 43 (6) ◽  
pp. 1047-1059 ◽  
Author(s):  
Maria P. Deichmiller ◽  
Frank J. Dixon
Keyword(s):  
Serum Protein ◽  
Half Life ◽  
Gamma Globulin ◽  
Serum Proteins ◽  
Growth Period ◽  
Unit Weight ◽  
Total Serum Protein ◽  
Total Serum ◽  
Beta Globulin ◽  
Newborn Animals

1. Incorporation of S35-labeled amino acids into serum proteins has been studied in neonatal and developing rabbits. It was found that, per unit weight, neonatal rabbits synthesized only about 1/36 of the gamma globulin, 1/7 of the beta globulin, ½ of the alpha globulin, and ⅛ of the albumin that an adult synthesized. The growing rabbit developed the ability to synthesize various serum proteins at different times. 2. Plasma volumes and serum protein concentrations were determined at different times during the growth period of the rabbit. Plasma volumes were found to be 1 and ½ times larger in newborn animals than in adults, with a gradual decline to the adult level. The total serum protein concentration at birth was about 60 to 65 per cent of the adult value and gradually increased with growth as the plasma volume decreased. 3. Half-lives of homologous albumin and gamma globulin were studied. The half-life of albumin in neonates was nearly twice as long as the half-life in adults, the latter value being reached at 1 month of age. The half-life of gamma globulin in neonates was more than twice as long as the half-life in adults and reached adult values at 2 to 3 months. 4. Attempts were made to alter serum protein metabolism. Gamma globulin synthesis early in life was augmented with antigen injections.

Serum Protein Values in Premature Infants

PEDIATRICS ◽  
1958 ◽  
Vol 21 (1) ◽  
pp. 26-26
Keyword(s):  
Human Milk ◽  
Serum Protein ◽  
Premature Infants ◽  
Gamma Globulin ◽  
Paper Electrophoresis ◽  
Total Serum Protein ◽  
Total Serum ◽  
Additional Protein ◽  
Protein Components ◽  
Effect Of Feeding

The present investigations were stimulated by an interest in the effect of feeding larger amounts of protein to premature infants than provided by human milk. Twenty-one premature infants between 1500 and 2400 gm in weight and 1 and 56 days of age were studied. The total serum protein and the serum-protein components were determined by paper electrophoresis. During an initial 8 weeks of the experiment the infants received human milk; later 10 of them received additional protein from a skimmed-milk preparation containing 89% casein. During the first 8 weeks the concentration of total protein in the serum decreased from 5.63 to 4.03 gm/100 ml. The decrease was in the albumin fraction (from 3 to 2 gm/100 ml) and the gammaglobulin fraction (from 1 to 0.5 gm/100 ml). The other globulin fractions remained constant. Casein added to the diet resulted in concentrations of total serum protein and serum albumin at higher levels, but the decrease with increasing age was not prevented. The decrease in gamma globulin, concurrent with increasing age, was not prevented by additional casein, on the contrary resulted in a further decrease. The author relates this to other studies in which a lower frequency of infection was found in premature infants fed on human milk than in infants fed on human milk with additional protein.

scholarly journals Selected haematological changes in Clarias gariepinus (Burchell, 1822) infected with a Trypansosoma sp. from the Vaal Dam, South Africa

2013 ◽  
Vol 80 (1) ◽  
Author(s):  
Maryke L. Ferreira ◽  
Annemarie Avenant-Oldewage
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Clarias Gariepinus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Plasma ◽  
Breeding Stock ◽  
Protein Levels ◽  
Total Plasma Protein ◽  
Haematological Changes

The use of haematological techniques to assess fish health is generally accepted. The aim of the current study was to determine selected haematological changes that occur in Clarias gariepinus (Burchell, 1822). infected with trypanosomes. Blood films were prepared according to standard techniques to confirm trypanosome infections and whole blood was collected, the serum and plasma separated, and prepared for albumin and total protein concentration analysis. Plasma albumin levels were significantly higher in infected wild caught fish than in uninfected wild caught fish and uninfected breeding stock. Serum albumin levels were significantly lower in infected wild caught fish when compared to uninfected breeding stock. The total plasma and serum protein levels were within the normal range for C. gariepinus, that is, 3 g – 6 g/100 mL. The total plasma protein levels varied significantly between the three groups. However, the total serum protein levels were only significantly different between uninfected breeding stock and uninfected wild caught fish, as well as uninfected breeding stock and infected wild caught fish. The total protein levels were significantly higher in infected wild caught fish than in the other groups, a possible indication of an infection or inflammatory host response.

scholarly journals Relation of the red blood globulin precipitation reaction to blood globulins. Salomon (Zeit. f. klin. Med, Bd. 99)

1925 ◽  
Vol 21 (2) ◽  
pp. 204-204
Author(s):  
J. Daikhovsky
Keyword(s):  
Red Blood Cells ◽  
Protein Content ◽  
Serum Protein ◽  
Sedimentation Rate ◽  
Blood Cells ◽  
Total Serum Protein ◽  
Total Serum ◽  
Precipitation Reaction ◽  
Healthy Individuals ◽  
Serum Globulin

After examining the serum globulin content of several sick and healthy individuals while determining the sedimentation rate of red blood cells (SR), Salomon found that in cases with normal SR the amount of globulin in the serum was about 30% of the total serum protein content; in patients with moderately accelerated SR the globulin content ranged between 36% and 51%; finally in cases with significantly accelerated SR the globulin content ranged between 37% and 70%.

scholarly journals The effect of dietary protein on reproduction in the mare. II. Growth of foals, body mass of mares and serum protein concentration of mares during the anovulatory, transitional and pregnant periods

1997 ◽  
Vol 68 (3) ◽  
Author(s):  
F.E. Van Niekerk ◽  
C.H. Van Niekerk
Keyword(s):  
Body Mass ◽  
Serum Protein ◽  
Total Protein ◽  
Protein Intake ◽  
National Research Council ◽  
The Body ◽  
Blood Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Protein Intake

The effect of 4 different diets, in terms of protein quantity and quality, on total serum protein (TSP), albumin and globulin was investigated. Non-pregnant mares that were not lactating (n = 36), pregnant mares that had foaled (n = 24) and their foals (n = 24) were used in this study. Daily total protein intake had no effect on blood protein concentrations in the mares. Total protein intake and quality (available essential amino-acids) did affect the body mass of mares during lactation. When mares were fed the minimum recommended (National Research Council 1989) total daily protein, foal mass decreased by approximately 25 % at weaning compared to the foals whose dams were on a higher level of protein intake. The TSP concentrations of foals at birth were on average 10 g/ℓ lower than those of the mares. Albumin concentrations of foals during the first 60 days of life were on average 2-3 g/ℓ lower than those of the mares. Globulin concentrations of foals were approximately 5 g/ℓ lower than those of mares at weaning.

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