Development of Hammondia heydorni in the Chorioallantoic Membrane of Chicken Embryos

1987 ◽  
Vol 73 (5) ◽  
pp. 1065 ◽  
Author(s):  
David S. Lindsay ◽  
Byron L. Blagburn
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Embryos

Altering the surface area available for transport of calcium from the eggshell does not affect calcium metabolism of chicken embryos

1991 ◽  
Vol 69 (12) ◽  
pp. 3084-3087
Author(s):  
Mary J. Packard
Keyword(s):  
Surface Area ◽  
Calcium Metabolism ◽  
Phosphorus Content ◽  
Chorioallantoic Membrane ◽  
Chicken Embryos ◽  
Calcium Magnesium ◽  
Inner Aspect ◽  
Chicken Eggs

The amount of contact between the chorionic surface of the chorioallantoic membrane and the inner aspect of the eggshell was reduced by causing formation of an extra air cell in chicken eggs on day 8 of incubation. This manipulation reduced the surface area available for transport of calcium from the eggshell to the embryo. Concentrations of calcium, magnesium, and phosphorus in serum were determined for embryos sampled on days 12, 15, and 18, and for hatchlings. Calcium, magnesium, and phosphorus content of residual yolks and yolk-free carcasses of hatchlings was also determined. The presence of an extra air cell did not lead to variation in any of these indices of calcium metabolism. One interpretation of these results is that embryos compensate for the reduction in surface area available for transport of calcium by increasing the transport of calcium across those areas of the chorioallantois that remain in contact with the eggshell.

scholarly journals Some observations on the adaptation ofEimeria tenella(local isolates) sporozoites on chicken embryos through chorioallantoic membrane

2006 ◽  
Vol 7 (1) ◽  
pp. 59
Author(s):  
M. Abdul Hafeez ◽  
Masood Akhtar ◽  
M. Mazhar Ayaz
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Embryos

scholarly journals Selective Binding of Lectins to Embryonic Chicken Vasculature

2003 ◽  
Vol 51 (5) ◽  
pp. 597-604 ◽  
Author(s):  
Shahla M. Jilani ◽  
Thomas J. Murphy ◽  
Shelley N.M. Thai ◽  
Anne Eichmann ◽  
Jackelyn A. Alva ◽  
...  
Keyword(s):  
Lens Culinaris ◽  
Developmental Stages ◽  
Chorioallantoic Membrane ◽  
Experimental System ◽  
In Ovo ◽  
Additional Advantage ◽  
Chicken Embryos ◽  
Lens Culinaris Agglutinin ◽  
Embryonic Chicken

Chicken embryos are an excellent model system for studies related to vascular morphogenesis. Development in ovo allows manipulations otherwise difficult in mammals, and the use of chicken-quail chimeras offers an additional advantage to this experimental system. Furthermore, the chicken chorioallantoic membrane has been extensively used for in vivo assays of angiogenesis. Surprisingly, few markers are available for a comprehensive visualization of the vasculature. Here we report the use of lectins for identification of embryonic chicken blood vessels. Nine lectins were evaluated using intravascular perfusion and directly on sections. Our results indicate that Lens culinaris agglutinin, concanavalin A, and wheat germ agglutinin can be used effectively for visualization of vessels of early chicken embryos (E2.5-E4). At later developmental stages, Lens culinaris agglutinin is a better choice because it displays equal affinity for the endothelia of arteries, veins, and capillaries. The findings presented here expand our understanding of lectin specificity in the endothelium of avian species and provide information as to the use of these reagents to obtain comprehensive labeling of the embryonic and chorioallantoic membrane vasculature.

scholarly journals Distinct Intensity of Host-Pathogen Interactions in Chlamydia psittaci- and Chlamydia abortus-Infected Chicken Embryos

2012 ◽  
Vol 80 (9) ◽  
pp. 2976-2988 ◽  
Author(s):  
Maria Braukmann ◽  
Konrad Sachse ◽  
Ilse D. Jacobsen ◽  
Martin Westermann ◽  
Christian Menge ◽  
...  
Keyword(s):  
Immune Response ◽  
Chorioallantoic Membrane ◽  
Chlamydia Psittaci ◽  
Internal Organs ◽  
Content Type ◽  
Chlamydia Abortus ◽  
Chicken Embryos ◽  
Related Proteins ◽  
Chicken Eggs ◽  
Infectious Potential

ABSTRACTFactors and mechanisms determining the differences in virulence and host specificity between the zoonotic agentsChlamydia psittaciandChlamydia abortusare still largely unknown. In the present study, two strains were compared for their invasiveness, virulence, and capability of eliciting an immune response in chicken embryos. On breeding day 10, embryonated chicken eggs were inoculated with 5 × 104inclusion-forming units. As shown by immunohistochemistry and quantitative real-time PCR,C. psittacidisplayed a significantly better capability of disseminating in the chorioallantoic membrane (CAM) and internal organs thanC. abortus. The higher infectious potential ofC. psittaciin birds was underlined by significantly higher mRNA expression rates of essential chlamydial genes, such asincA,groEL(in CAM, liver, and spleen),cpaf, andftsW(in CAM). Although the immune responses to both pathogens were similar,C. psittacielicited higher macrophage numbers and a stronger expression of a subset of immune-related proteins. The data imply that invasiveness ofChlamydiaspp. and propagation in the host are not solely dependent on the level of host immune response but, even to a greater extent, on the expression of bacterial factors related to virulence. The fact thatC. psittacihas coped far better thanC. abortuswith the avian embryo's response by upregulating essential genes may be a key to understanding the mechanisms underlying host adaptation and etiopathology.

scholarly journals Antagonistic Effects of Suramin Against the Venom of Snake, Echis Carinatus, on the Circulatory System of Developing Chicken Embryos

2019 ◽  
Vol 13 (1) ◽  
pp. 13-18
Author(s):  
Zahra Hajari ◽  
◽  
Behrooz Fathi ◽  
Zohreh Saadatfar ◽  
Abbas Zare ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Circulatory System ◽  
Dependent Manner ◽  
Inhibitory Effects ◽  
Antagonistic Effects ◽  
Chicken Embryos ◽  
Time To Death ◽  
Hemorrhagic Activity ◽  
Echis Carinatus ◽  
Dose Dependent

Background: The snake, Echis carinatus, one of the most venomous snakes in Asia, possesses a deadly hemotoxic venom. It has been reported that suramin, an anti-trypansomiasis drug, can inhibit the toxic effects of some snake venoms. This study was conducted to evaluate the possible antagonistic effects of suramin against the hemorrhagic activity of the venom from an Iranian snake, Echis carinatus, in developing chicken embryos. Methods: One day old fertile eggs (n=250) were incubated for six days at 37ºC and 60% humidity. Paper discs (5 mm diameter) containing different concentrations of E. carinatus venom (5, 10, 20 & 30 μg) were placed on the chorioallantoic membrane over the major bilateral vein and were left in place until hemorrage occurred and the embryos died. Results: We found the standard hemorrhagic dose (SHD) of E. carinatus venom to be (5 μg/disc). Various concentrations (5, 10 & 20 μg) of suramin were tested against SHD of E. carinatus venom in different protocols. Control experiments were performed with the buffered saline solution, venom and suramin individually. The results demonstrated that suramin at 5, 10 and 20 μg significantly (P<0.05) delayed time to death (717, 521 & 208 min) of embryos poisoned with E. carinatus venom in a dose-dependent manner. Conclusion: Suramin exerted protective and inhibitory effects against the deadly E. carinatus venom, and therefore, may potentially offer future therapeutic applications against poisoning with E. carinatus venom.

scholarly journals In vivo evidence of angiogenesis inhibition by β2-glycoprotein I subfractions in the chorioallantoic membrane of chicken embryos

2021 ◽  
Vol 54 (3) ◽  
Author(s):  
C.M. Baldavira ◽  
L.F. Gomes ◽  
L.T. De La Cruz ◽  
D.A. Maria ◽  
V.L. Capelozzi
Keyword(s):  
Chorioallantoic Membrane ◽  
Chicken Embryos ◽  
Angiogenesis Inhibition ◽  
Glycoprotein I

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

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