scholarly journals Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Dan-Feng Xu ◽  
Guo-Xin Qu ◽  
Shi-Gui Yan ◽  
Xun-Zi Cai
Keyword(s):  
Animal Model ◽  
Femoral Head ◽  
Rabbit Model ◽  
Mineral Apposition Rate ◽  
Histological Evaluation ◽  
Pulsed Ultrasound ◽  
Mineral Density ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ct Analysis

Microbubbles magnify the acoustic pressure of low-intensity pulsed ultrasound (LIPUS) and may enhance its bioeffect for diagnostic and therapeutic purposes. This study compared the effect of this novel microbubble-mediated ultrasound (MUS) with that of the traditional LIPUS on osteogenesis and neovascularization in a rabbit model of steroid-associated osteonecrosis. We hypothesized that MUS might outweigh LIPUS on promoting osteogenesis and neovascularization in steroid-associated osteonecrosis. The bilateral femoral head necrosis was induced by lipopolysaccharide and methylprednisolone in the rabbits. The indices of bone mineral density (BMD), trabecular number, maximal loading strength, and mineral apposition rate were analyzed, demonstrating that the animal model of steroid-associated osteonecrosis was successfully established. Both the MUS group (GM) and the LIPUS group (GL) were insonated 20 min daily for six weeks. GM received an extra intracapsular injection of microbubbles before insonation every other day. Fluorescence bone labeling, Micro-CT Analysis, biomechanical test, quantitative real-time PCR, Western blot analysis, and histological evaluation were performed for comparing GM with GL. The results demonstrated a 39% higher mineral apposition rate in GM compared with GL. The BMD and the maximal loading strength of femoral head of GM increased by 4.3% and 27.8% compared to those of GL, respectively. The mRNA and protein expression of BMP-2 and VEGF were also significantly higher in GM. The number of blood vessels of GM was 65% greater than that of GL. MUS is more potent than LIPUS in enhancing osteogenesis, neovascularization, and biomechanical strength of femoral head in the animal model of steroid-associated osteonecrosis. Without increasing the intensity of insonation or the risk of tissue damage, MUS is better for inhibiting the process of steroid-associated osteonecrosis.

Low-intensity Pulsed Ultrasound Does Not Enhance Distraction Callus in a Rabbit Model

2007 ◽  
Vol 459 ◽  
pp. 237-245 ◽  
Author(s):  
Kenneth F Taylor ◽  
Bahman Rafiee ◽  
John E Tis ◽  
Nozumu Inoue
Keyword(s):  
Rabbit Model ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low-intensity pulsed ultrasound reduces periodontal atrophy in occlusal hypofunctional teeth

2017 ◽  
Vol 87 (5) ◽  
pp. 709-716 ◽  
Author(s):  
Yuki Kasahara ◽  
Risa Usumi-Fujita ◽  
Jun Hosomichi ◽  
Sawa Kaneko ◽  
Yuji Ishida ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Bhlh Transcription Factor ◽  
Mrna Levels ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Qrt Pcr ◽  
Low Intensity ◽  
Normal Occlusion ◽  
Ct Analysis

ABSTRACT Objective: To clarify whether low-intensity pulsed ultrasound (LIPUS) exposure has recovery effects on the hypofunctional periodontal ligament (PDL) and interradicular alveolar bone (IRAB). Materials and Methods: Twelve-week-old male Sprague-Dawley rats were divided into three groups (n = 5 each): a normal occlusion (C) group, an occlusal hypofunction (H) group, and an occlusal hypofunction group subjected to LIPUS (HL) treatment. Hypofunctional occlusion of the maxillary first molar (M1) of the H and HL groups was induced by the bite-raising technique. Only the HL group was irradiated with LIPUS for 5 days. The IRAB and PDL of M1 were examined by microcomputed tomography (micro-CT) analysis. To quantify mRNA expression of cytokines involved in PDL proliferation and development, real-time reverse transcription quantitative PCR (qRT-PCR) was performed for twist family bHLH transcription factor 1 (Twist1), periostin, and connective tissue growth factor (CTGF) in the PDL samples. Results: Micro-CT analysis showed that the PDL volume was decreased in the H group compared with that of the C and HL groups. Both bone volume per tissue volume (BV/TV) of IRAB was decreased in the H group compared with that in the C group. LIPUS exposure restored BV/TV in the IRAB of the HL group. qRT-PCR analysis showed that Twist1, periostin, and CTGF mRNA levels were decreased in the H group and increased in the HL group. Conclusion: LIPUS exposure reduced the atrophic changes of alveolar bone by inducing the upregulation of periostin and CTGF expression to promote PDL healing after induction of occlusal hypofunction.

Effect of low-intensity pulsed ultrasound on fracture callus mineral density and flexural strength in rabbit tibial fresh fracture

2010 ◽  
Vol 15 (2) ◽  
pp. 240-244 ◽  
Author(s):  
Kazem Shakouri ◽  
Bina Eftekharsadat ◽  
Mohammad Reza Oskuie ◽  
Jafar Soleimanpour ◽  
Mohammad Kazem Tarzamni ◽  
...  
Keyword(s):  
Flexural Strength ◽  
Pulsed Ultrasound ◽  
Mineral Density ◽  
Fracture Callus ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Fresh Fracture

Effect of Low-Intensity Pulsed Ultrasound After Autologous Adipose-Derived Stromal Cell Transplantation for Bone-Tendon Healing in a Rabbit Model

2019 ◽  
Vol 47 (4) ◽  
pp. 942-953 ◽  
Author(s):  
Can Chen ◽  
Tao Zhang ◽  
Fei Liu ◽  
Jin Qu ◽  
Yang Chen ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Volume Fraction ◽  
Treatment Time ◽  
Rabbit Model ◽  
The Other ◽  
Pulsed Ultrasound ◽  
Trabecular Thickness ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background: Low-intensity pulsed ultrasound (LIPUS), as a safe biophysiotherapy, can enhance bone-tendon (B-T) healing in vivo and induce osteogenic or chondrogenic differentiation of mesenchymal stromal cells in vitro. This study aimed to determine whether LIPUS can improve the efficacy of transplanted mesenchymal stromal cells on B-T healing. Hypothesis: LIPUS can induce lineage-specific differentiation of transplanted adipose-derived stromal cells (ASCs) at the B-T healing site, thus resulting in superior healing quality when compared with LIPUS or ASCs alone. Study Design: Controlled laboratory study. Methods: A total of 112 mature rabbits with partial patellectomy in the hindlimb were randomly assigned into mock sonication without ASCs (control), ultrasonication without ASCs (LIPUS), mock sonication with ASCs (ASCs), and ultrasonication with ASCs (LIPUS + ASCs). The treatment time of the mock sonication or ultrasonication was 20 minutes per day. Autologous ASCs were transplanted to the healing site by fibrin glue during the operation, and LIPUS was delivered daily starting at postoperative day 3 until euthanasia. The patella–patellar tendon junctions were postoperatively harvested at 8 and 16 weeks for radiological, histological, and mechanical evaluations. Additionally, 9 animals were used for ASC tracking with mCherry protein. Results: Radiologically, there was more new bone formation and remodeling in the LIPUS + ASCs group as compared with the other groups. Synchrotron radiation micro–computed tomography showed that the LIPUS + ASCs group significantly increased bone volume fraction, trabecular thickness, and trabecular number at the healing site as compared with the other groups at postoperative 8 weeks ( P < .05 for all). Histologically, immunohistochemical staining confirmed that the transplanted mCherry-ASCs can differentiate into osteoblasts and fibrochondrocytic-like cells. Meanwhile, as compared with the other groups, the LIPUS + ASCs group showed more formation and maturity of the fibrocartilage layer and new bone at postoperative weeks 8 and 16 ( P < .05 for all). Biomechanically, the LIPUS + ASCs group showed significantly higher failure load and stiffness versus the other groups at postoperative weeks 8 and 16 ( P < .05 for all). Conclusion: Autologous ASC transplantation stimulated with LIPUS can result in superior B-T healing quality when compared with LIPUS or ASCs alone. Clinical Relevance: This study demonstrates the effectiveness of using ASC transplantation stimulated with LIPUS for B-T healing and provides a foundation for future clinical studies.

Low-intensity pulsed ultrasound repair in mandibular condylar cartilage injury rabbit model

2019 ◽  
Vol 104 ◽  
pp. 60-66 ◽  
Author(s):  
Hang-yu Zhou ◽  
Qiang Li ◽  
Jian-xiong Wang ◽  
Yu-jie Xie ◽  
Shi-qi Wang ◽  
...  
Keyword(s):  
Rabbit Model ◽  
Cartilage Injury ◽  
Pulsed Ultrasound ◽  
Condylar Cartilage ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Mandibular Condylar Cartilage
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