scholarly journals Presence of .BETA.-lactamase and penicillin acylase in a Pseudomonas sp. utilizing benzylpenicillin as a carbon source.

1981 ◽  
Vol 27 (6) ◽  
pp. 499-503 ◽  
Author(s):  
JÜRGEN JOHNSEN
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty

2018 ◽  
Vol 114 ◽  
pp. 512-519 ◽  
Author(s):  
Lucas G. Cespedes ◽  
Rafael A.T.P.S. Nahat ◽  
Thatiane T. Mendonça ◽  
Rafaela R. Tavares ◽  
Edmar R. Oliveira-Filho ◽  
...  

1987 ◽  
Vol 33 (6) ◽  
pp. 534-540 ◽  
Author(s):  
Rodolphe Boivin ◽  
Hélène Lebeuf ◽  
Patrice Dion

An octopine-utilizing bacterium isolated from soil, named strain 92, was identified to the genus level as a nonfluorescent Pseudomonas. Utilization of octopine by strain 92 depended upon the presence of an alternative carbon source, while octopinic acid was not utilized. A mutant derivative, strain RB100, acquired simultaneously the capacity to utilize octopine as the sole carbon and nitrogen source and octopinic acid as the nitrogen source. The mutation rate was estimated to be about 10−8 per cell per generation. Both the wild type and mutant carried the 68.5-kilobase cryptic plasmid pDLB 1. When the plasmid present in strain RB100 was labelled with Tn5 and transferred to strain 92, the phenotype of the recipient was not modified with respect to octopine utilization, suggesting that this phenotype was not determined by pDLB1. This suggestion was confirmed by using unstable derivatives of pDLB1 to cure strain RB100 and showing that the cured strain was not affected in its capacity to utilize opines.


2012 ◽  
Vol 2 (3) ◽  
Author(s):  
Shanmugam Senthilkumar ◽  
Muthiah Perumalsamy ◽  
Harinarayan Prabhuy ◽  
Chiya AhmedBasha ◽  
Narayan Anantharaman

AbstractResponse surface methodology (RSM) involving the central composite design (CCD) was employed to optimize three important process variables for the decolourization of synthetic dye solutions containing Remazol Turquoise Blue (RTB) and Reactive Black 5 (RB5) with isolated bacterial strain Pseudomonas sp. The interaction between three variables i.e. Initial concentration of dye, carbon source and nitrogen source were studied and modeled. According to the Analysis of variance (ANOVA) results the predicted results were found to be in good agreement with experimental results (R 2: 0.9726; Adj R 2: 0.9480 for RTB and R 2: 0.9789; Adj R 2: 0.9750 for RB5) which indicated excellent evaluation of experimental data from the second order polynomial regression model. Mathematical models were developed by the proposed system, for each process variable showed the effect of each factor and their interactions on biodecolourization process. The optimum concentrations of Dye, Carbon source, and Nitrogen source were found to be 20 mgL−1, 1.5 g/L and 1.5 g/L, respectively for RTB and RB5 to obtain maximum dye removing capacity. Predicted values were validated with experimental results, which indicated appropriateness of the employed model and the success of RSM.


2009 ◽  
Vol 61 (2) ◽  
pp. 159-164 ◽  
Author(s):  
B. Jovcic ◽  
Jelena Begovic ◽  
Jelena Lozo ◽  
L. Topisirovic ◽  
Milan Kojic

Pseudomonas sp. ATCC19151 harbors a gene encoding a putative alkylsulfatase (sdsA). Here we report a growth ability of this strain in minimal media containing 0.5, 0.75, and 1% sodium dodecyl sulfate as the sole carbon source. The most prominent growth was detected for the minimal medium with 0.5% SDS, so this concentration of SDS was used to monitor Pseudomonas sp. ATCC19151 SDS biodegradation dynamics. Bacterial growth coincided with the disappearance of SDS. Antibiotic susceptibility was tested as well. Pseudomonas sp. ATCC19151 was resistant to six out of nine tested antibiotics, including ampicillin, tetracycline, chloramphenicol, tobramycin, nalidixic acid, and gentamycin.


2021 ◽  
Vol 9 (2) ◽  
pp. 8-12
Author(s):  
A.J. Sufyan ◽  
S. Ibrahim ◽  
A. Babandi ◽  
Hafeez Muhammad Yakasai

Butachlor is a chloroacetanilide herbicide that is selective in action and commonly used for pre-emergence control of weeds. It is believed to have range of toxicity from acute to chronic and also can cause DNA strand breaks and chromosomal aberrations in humans. This study was aimed at characterizing the potential of previously isolated bacteria for butachlor degradation. The isolates from culture collection, labelled A-F were screened for butachlor degradation on Bushnell Hass agar media with butachlor as a sole carbon source. Isolate A (molybdenum-reducing and aniline-degrading Pseudomonas sp.) was observed to grow best and tolerated the highest concentration of butachlor supplemented in the media after 72 h of incubation at 37 ℃. Characterization study revealed that the Pseudomonas sp. can utilize and grow with butachlor at optimum pH between 6.0 - 6.5, temperature between 30 – 37 ℃ and can tolerate up to 600 mg/L butachlor concentration with increase in growth as inoculum size increases. Additionally, this bacterial strain shows no lag phase regardless of the concentration of the herbicide used and reach its maximum growth after 24 h of incubation. The ability of this isolate to tolerate and utilize butachlor as sole carbon source makes it suitable for future bioremediation of this toxicant.


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