Effect of Baicalein on Matrix Metalloproteinases and Durability of Resin-Dentin Bonding

2018 ◽  
Vol 43 (4) ◽  
pp. 426-436 ◽  
Author(s):  
J Li ◽  
B Chen ◽  
N Hong ◽  
S Wu ◽  
Y Li
Keyword(s):  
Matrix Metalloproteinases ◽  
Bonding Strength ◽  
Resin Composite ◽  
Artificial Saliva ◽  
Adhesive System ◽  
Inhibitory Effect ◽  
Dentin Bonding ◽  
Microtensile Testing ◽  
Etch And Rinse

SUMMARY Objective: In an attempt to increase resin-dentin bonding quality, this study used baicalein as a preconditioner in an etch-and-rinse adhesive to evaluate its effect on matrix metalloproteinases (MMPs) and adhesive durability. Methods: As a MMP inhibitor and potential collagen cross-linking agent, baicalein was used as a preconditioner in an etch-and-rinse adhesive system. The degree of conversion was evaluated by Fourier-transform infrared spectroscopy. EnzChek gelatinase/collagenase assay kits were then used to detect the MMP inhibitory effect of different concentrations of baicalein (0.1, 0.5, 2.5, and 5.0 μg/mL) on dentin powders. During in vitro bonding procedures, flat dentin surfaces on sound third molars were preconditioned with 2.5 μg/mL baicalein after being acid-etched; this step was followed by continuation of adhesive processes and build-up of resin composite. After resin-dentin stick preparation, bonding strength, failure mode, and interface nanoleakage were respectively evaluated via microtensile testing, stereomicroscopy, and field emission scanning electron microscopy either immediately or after storage in artificial saliva for three or six months. Data were analyzed by two-way analysis of variance and Tukey test (α=0.05). Results: Baicalein at a concentration of 0-5.0 μg/mL did not influence the conversion of adhesives. However, it inhibited the activities of dentin-bond gelatinase and collagenase, especially at a concentration of 2.5 μg/mL, while effectively increasing microtensile bonding strength and decreasing nanoleakage in vitro, both immediately and after aging. Conclusions: Baicalein used as preconditioner in an etch-and-rinse adhesive system has an anti-MMP function and effectively improves resin-dentin bonding durability in vitro, which has potential value in clinical bonding procedures.

scholarly journals Influence of Chlorhexidine and/or Ethanol Treatment on Bond Strength of an Etch-and-rinse Adhesive to Dentin: An In Vitro and In Situ Study

2014 ◽  
Vol 39 (1) ◽  
pp. 64-71 ◽  
Author(s):  
DMS Simões ◽  
RT Basting ◽  
FLB Amaral ◽  
CP Turssi ◽  
FMG França
Keyword(s):  
Bond Strength ◽  
Resin Composite ◽  
Adhesive System ◽  
Dentin Surface ◽  
In Vitro Aging ◽  
Etch And Rinse ◽  
Bond Interface ◽  
Vitro Model

SUMMARY The aim of this study was to evaluate the effect of a chlorhexidine and/or ethanol application on the bond strength of an etch-and-rinse, hydrophobic adhesive system either under in vitro aging or in situ cariogenic challenge. The dentin surface of 36 human third molars were flattened and allocated into four groups to be treated with chlorhexidine, ethanol, or chlorhexidine + ethanol or left unexposed to any solution (control) (n=9). Then, a resin composite restoration was made on the dentin surface and longitudinal sticks were obtained. Sticks from each tooth were assigned to three test conditions: stored in water in vitro for 24 hours, stored in water in vitro for 6 months, or worn in situ for 14 days. During in situ wear time, a high-cariogenic challenge condition was simulated. Specimens were tested for microtensile bond strength (μTBS). Multivariate analysis of variance and Tukey's test showed that chlorhexidine, ethanol, or chlorhexidine + ethanol did not affect the μTBS. The in vitro μTBS values were significantly lower for the specimens stored for 6 months than for those stored for 24 hours. Intermediate μTBS values were shown by the specimens worn in situ. Thus, use of chlorhexidine and/or ethanol was incapable of containing the degradation at the bond interface in the in vitro model. The in situ model was capable of reducing bond strength similarly to the in vitro/6 months model. Despite this, the in situ bond strength was still similar to that of the in vitro/24-hour model.

scholarly journals The Effect of Biodentine Maturation Time on Resin Bond Strength When Aged in Artificial Saliva

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Ruba M. Mustafa ◽  
Suhad J. Al-Nasrawi ◽  
Abtesam I. Aljdaimi
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Resin Composite ◽  
Artificial Saliva ◽  
Adhesive System ◽  
Failure Patterns ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Post Hoc ◽  
Composite Restoration

Biodentine is a calcium silicate cement (CSC) that has been broadly applied in vital pulp therapy. The quality of the Biodentine-composite bond has a significant effect on the longevity of the definitive restoration. The aim of this study is to investigate the shear bond strength (SBS) between Biodentine and composite restoration at different maturation times of Biodentine aged in artificial saliva. Fifteen Biodentine discs were allocated into three groups (n = 5) based on the timeframe of performance of composite restoration: immediate (after 12  min), after 14 days, and after 28 days of Biodentine maturation. Total etch and rinse adhesive system and bulk-fill regular resin composite were used. The shear bond strength and the failure pattern were assessed. One-way ANOVA with the Bonferroni post hoc test was applied for statistical analysis at p < 0.05 . The highest (32.47 ± 8.18 MPa) and the lowest (4.08 ± 0.81 MPa) SBS values were recorded for 14 days and 12 min groups, respectively. Significant statistical differences were reported among the groups, and a high statistically significant difference was found between the immediate group and the other groups. Adhesive failure patterns were evident in all groups. More clinically acceptable bond strength between the Biodentine and overlaid composite restoration is at 14 days after Biodentine maturation. Delaying the coverage of Biodentine later than 14 days may significantly reduce the SBS. Using the artificial saliva as an aging medium may affect the SBS between Biodentine and composite material.

Adhesive Bond Strengths Using Self- and Light-Cured Composites

2009 ◽  
Vol 10 (6) ◽  
pp. 25-32 ◽  
Author(s):  
Ricardo Walter ◽  
Georgia V. Macedo ◽  
Gustavo M. S. Oliveira ◽  
Edward J. Swift
Keyword(s):  
Resin Composite ◽  
Adhesive Bond ◽  
Adhesive System ◽  
Resin Composites ◽  
Microtensile Bond Strength ◽  
Microtensile Testing ◽  
Etch And Rinse ◽  
Occlusal Surfaces ◽  
One Step ◽  
Bond Strengths

Abstract Aim To evaluate the microtensile bond strength (μTBS) of four adhesive systems to dentin, using self- and light-cured resin composites. Methods and Materials Crowns of human molars were separated from the roots, and the occlusal surfaces were ground to obtain flat superficial dentin. Three etch-and-rinse adhesives—All-Bond 2, One-Step Plus, and OptiBond Solo Plus—and one self-etching primer system, Peak SE, were evaluated. Each adhesive group was divided into two subgroups according to the type of resin composite used. A self-cured (Bisfil 2B) or light-cured (Filtek Supreme Plus) resin composite build-up was incrementally inserted to the dentin after each adhesive system was applied. The bonded specimens were stored in water for 24 h and sectioned into beams. Microtensile testing was done, and the data were subjected to ANOVA and Fisher's PLSD test. Results The μTBS of All-Bond 2 and One- Step Plus was not affected by the type of resin composite used (p=0.3131 and p=0.1562, respectively). The μTBS of OptiBond Solo Plus was significantly reduced when used with selfcured resin composite (p<0.0001). Peak SE formed no bond of self-cured resin composite to dentin. Conclusions Some adhesives do not effectively bond self-cured resin composite to dentin. Clinical Significance Incompatibility between adhesives with low pH and certain self-cured resin composites can cause clinical debonding of restorations. Citation Walter R, Macedo GV, Oliveira GMS, Swift Jr EJ. Adhesive Bond Strengths Using Self- and Light-Cured Composites. J Contemp Dent Pract [Internet]. 2009 Nov; 10(6):025-032. Available from: http://www.thejcdp.com/journal/ view/volume10-issue6-walter.

scholarly journals Increased Durability of Resin-Dentin Bonds Following Cross-Linking Treatment

2015 ◽  
Vol 40 (5) ◽  
pp. 533-539 ◽  
Author(s):  
DLS Scheffel ◽  
CC Delgado ◽  
DG Soares ◽  
FG Basso ◽  
CA de Souza Costa ◽  
...  
Keyword(s):  
Bond Strength ◽  
Resin Composite ◽  
Artificial Saliva ◽  
Deionized Water ◽  
Lower Amount ◽  
Control Group ◽  
Single Bond ◽  
Microtensile Testing ◽  
Etch And Rinse ◽  
Bond Degradation

SUMMARY Objectives: This study evaluated the long-term effect of carbodiimide treatments of acid-etched dentin on resin-dentin bond strength of a simplified etch-and-rinse adhesive system. Methods: Forty-eight sound third molars were divided into three groups (n=16) according to the dentin treatment: G1: deionized water; G2: 0.5 mol/L 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) applied for 30 seconds; and G3: 0.5 mol/L EDC applied for 60 seconds. Flat dentin surfaces were produced, etched with 37% phosphoric acid for 15 seconds, and then treated with deionized water for 60 seconds or with 0.5 mol/L EDC for 30 or 60 seconds prior to the application of Single Bond 2. Crowns were restored with resin composite, and beam specimens were prepared for microtensile testing. The beams from each group were tested 24 hours or 6 or 12 months after the adhesive procedures. One slab from each tooth was prepared and analyzed for nanoleakage. Bond strength (MPa) data were submitted to analysis of variance and Tukey test (α=0.05). Results: The treatment of dentin with 0.5 mol/L EDC for 30 seconds (24.1±6.2 MPa) and 60 seconds (25.5±5.1 MPa) did not negatively affect the immediate bond strength of Single Bond 2 when compared to the control group (24.6±7.3 MPa). Additionally, EDC prevented resin-dentin bond degradation after 12 months in artificial saliva for both periods of treatment. An increased accumulation of silver ions was seen for the control group over time, while a much lower amount of silver grains was observed for the EDC-treated groups. Conclusions: 0.5 mol/L EDC was able to prevent resin-dentin bond degradation after 12 months, especially when applied for 60 seconds.

scholarly journals Micro-computed tomography evaluation of microleakage of Class II composite restorations: An in vitro study

2018 ◽  
Vol 12 (03) ◽  
pp. 369-374 ◽  
Author(s):  
Angelo Zavattini ◽  
Manuele Mancini ◽  
James Higginson ◽  
Federico Foschi ◽  
Guido Pasquantonio ◽  
...  
Keyword(s):  
Null Hypothesis ◽  
Resin Composite ◽  
In Vitro Study ◽  
Adhesive System ◽  
Class Ii ◽  
Micro Computed Tomography ◽  
Flowable Composite ◽  
Agno3 Solution ◽  
Etch And Rinse

ABSTRACT Objective: The aim of this study is to investigate the microleakage attained with three resin-based material used to restore deep Class II cavities. A null hypothesis was chosen: there is no difference in microleakage among the tested materials. Materials and Methods: A total of 30 Class II cavities were prepared in freshly extracted molars with the proximal mesial and distal margins located, respectively, 1.5 mm apically and 1.5 mm coronally to the cementum-enamel junction. Restorations were completed using a three-step enamel-dentin adhesive system “Etch and Rinse,” margins were relocated using a micro-hybrid, preheated, or flowable composite and restorations were then completed using a conventional composite. All samples were coated with nail varnish with the exception of an area along the margins and apex was sealed using epoxide cement and then thermocycled (30-s dwell time, 5°C/55°C, 1000 cycles). A 50% ammoniac AgNO3 solution was used as tracer according to Tay's protocol. The microleakage analysis was performed using a microtomography system Sky-scan 1072 (SKYSCAN, Kartuizersweg 3B 2550, Konitch, Belgium). Results: The mean microleakage of all the tested materials showed greater leakage in the cementum margins; flowable composite exhibit greater leakage among the groups. Significant differences (P < 5%) within groups in both enamel and dentin margins were present. None of the tested materials eliminated marginal microleakage. Preheated composite showed significantly lesser microleakage. Conclusion: Tested materials showed statistical differences in microleakage; thus, the null hypothesis has been rejected. Within the limitations of the present experimental procedure, it can be concluded that flowable resin composite should be avoided at the dentin/cementum margin.

scholarly journals The inhibitory effect of carboxyl-terminated polyamidoamine dendrimers on dentine host-derived matrix metalloproteinases in vitro in an etch-and-rinse adhesive system

2019 ◽  
Vol 6 (10) ◽  
pp. 182104
Author(s):  
Qian Wu ◽  
Tiantian Shan ◽  
Manduo Zhao ◽  
Sui Mai ◽  
Lisha Gu
Keyword(s):  
Matrix Metalloproteinases ◽  
Organic Matrix ◽  
Adhesive System ◽  
Collagen Fibrils ◽  
Fourth Generation ◽  
Etch And Rinse ◽  
Polyamidoamine Dendrimers ◽  
Pre Treatment

The biomimetic remineralization of collagen fibrils has increased interest in restoring the demineralized dentine generated by dental caries. Carboxyl-terminated polyamidoamine dendrimers (PAMAM-COOH), hyperbranched polymeric macromolecules, can act as non-collagenous proteins to induce biomimetic remineralization on a dentine organic matrix. However, in vivo remineralization is an extremely time-consuming process; before complete remineralization, demineralized dentine collagen fibrils are susceptible to degradation by host-derived matrix metalloproteinases (MMPs). Therefore, we examined the effect of fourth-generation PAMAM-COOH (G4-PAMAM-COOH) on the collagenolytic activities of endogenous MMPs, the interaction between G4-PAMAM-COOH and demineralized dentine collagen and the influence of G4-PAMAM-COOH pre-treatment on resin–dentine bonding. G4-PAMAN-COOH not only inhibited exogenous soluble rhMMP9 but also hampered the proteolytic activities of dentine collagen-bound MMPs. Cooperated with the results of G4-PAMAM-COOH absorption and desorption, FTIR spectroscopy provided evidence for the exclusive electrostatic interaction rather than hydrogen or covalent bonding between G4-PAMAM-COOH and dentine collagen. Furthermore, G4-PAMAM-COOH pre-treatment showed no damage to resin–dentine bonding because it did not significantly decrease the elastic modulus of the demineralized dentine, degree of conversion, penetration of the adhesive into the dentinal tubules or ultimate tensile strength. Thus, G4-PAMAM-COOH can effectively inactivate MMPs, retard the enzymolysis of collagen by MMPs and scarcely influence the application of resin–dentine bonding.

scholarly journals In Vivo and In Vitro Effects of Chlorhexidine Pretreatment on Immediate and Aged Dentin Bond Strengths

2016 ◽  
Vol 41 (3) ◽  
pp. 258-267 ◽  
Author(s):  
Z Gunaydin ◽  
AR Yazici ◽  
ZC Cehreli
Keyword(s):  
Bond Strength ◽  
Resin Composite ◽  
Adhesive System ◽  
Etch And Rinse ◽  
Bonferroni Test ◽  
Bond Strengths ◽  
In Vitro Effects ◽  
Self Etch

SUMMARY This study evaluated the effect of 2% chlorhexidine (CHX) pretreatment of dentin on the immediate and aged microtensile bond strength (μTBS) of different adhesives to dentin in vivo and in vitro. Class I cavities were prepared in 80 caries-free human third molars of 40 patients in a split-mouth fashion. In each tooth pair, one tooth received 2% CHX pretreatment after which both teeth were randomly assigned to one of the following groups with respect to the type of adhesive system applied: Adper Single Bond 2 (etch-and-rinse), Clearfil SE Bond (two-step self-etch), Clearfil S3 Bond (one-step self-etch), and Adper Prompt-L-Pop (all-in-one self-etch). The teeth were restored with resin composite and extracted for μTBS testing either immediately or after six months in function. In vitro specimen pairs were prepared as with the clinical protocol in intact, freshly extracted human molars, and thereafter, subjected to testing immediately or after 5000× thermocycling. Data were analyzed with four-way analysis of variance (ANOVA). Bonferroni test was utilized for pair-wise comparisons. The immediate bond strength values were significantly higher than “aged” ones for all tested adhesives (p=0.00). The in vitro immediate bond strength values were statistically higher than in vivo bond strength values (p&lt;0.05). While the bond strength of in vitro aged, CHX-treated samples were higher than their in vivo counterparts (p&lt;0.05), no difference was observed in non-CHX treated groups (p&gt;0.05). In the absence of CHX pretreatment, all adhesives showed significantly higher immediate bond strength values than CHX-treated groups, while all “aged”, non-pretreated adhesives exhibited significantly lower bond strength values (both p&lt;0.05). By contrast, chlorhexidine pretreatment resulted in significantly higher aged bond strengths, regardless of the adhesive system and testing condition. Aging-associated decline in dentin bond strength of etch-and rinse and self-etch adhesives can be counteracted by chlorhexidine application.

scholarly journals Chlorhexidine Preserves Dentin Bond in vitro

2007 ◽  
Vol 86 (1) ◽  
pp. 90-94 ◽  
Author(s):  
M.R.O. Carrilho ◽  
R.M. Carvalho ◽  
M.F. de Goes ◽  
V. di Hipólito ◽  
S. Geraldeli ◽  
...  
Keyword(s):  
Bond Strength ◽  
Protease Inhibitors ◽  
Resin Composite ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Hybrid Layer ◽  
Adhesive Resin ◽  
Bond Stability ◽  
Etch And Rinse

Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted third molars were sectioned into two halves. One half was customarily restored (etch-and-rinse adhesive/resin composite), and the other was treated with 2% chlorhexidine after being acid-etched before restoration. Specimens were stored in artificial saliva with/without protease inhibitors. Microtensile bond strengths and failure mode distribution under SEM were analyzed immediately after specimens’ preparation and 6 months later. With chlorhexidine, significantly better preservation of bond strength was observed after 6 months; protease inhibitors in the storage medium had no effect. Failure analysis showed significantly less failure in the hybrid layer with chlorhexidine, compared with controls after 6 months. In conclusion, this in vitro study suggests that chlorhexidine might be useful for the preservation of dentin bond strength.

scholarly journals In Vitro Effects of Resin Infiltration on Enamel Erosion Inhibition

2015 ◽  
Vol 40 (5) ◽  
pp. 492-502 ◽  
Author(s):  
GC Oliveira ◽  
AP Boteon ◽  
FQ Ionta ◽  
MJ Moretto ◽  
HM Honório ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Adhesive System ◽  
Dental Erosion ◽  
Deep Penetration ◽  
Resin Infiltration ◽  
Fluorescence Confocal Microscopy ◽  
In Vitro Effects

SUMMARY Resin-based materials that show promising effects for preventing the progression of erosion have been studied. This in vitro study evaluated the effects of applying resin-based materials, including resin infiltration, on previously eroded enamel subjected to erosive challenges. The influence of enamel surface etching prior to application of the material was also studied. Bovine enamel blocks were immersed in hydrochloric acid (HCl), 0.01 M (pH 2.3), for 30 seconds in order to form a softened erosion lesion. The blocks were then randomly divided into nine groups (n=12) and treated as follows: C = control without treatment; Hel = pit & fissure resin sealant (Helioseal Clear); Adh = two-step self-etching adhesive system (AdheSe); Tet = two-step conventional adhesive system (Tetric N-bond); and Inf = infiltrant (Icon). The Helno, Adhno, Tetno, and Infno groups received the same materials without (or with no) surface conditioning. The depth of the material's penetration into softened erosion lesions was qualitatively analyzed using reflection and fluorescence confocal microscopy. After application of the materials, the blocks were immersed in HCl for two minutes; this step was followed by immersion in artificial saliva for 120 minutes four times a day for five days (erosive cycling). Both the enamel alteration and material thickness were analyzed using profilometry, and the results were submitted to Kruskal-Wallis and Dunn tests (p&gt;0.05). Images from the confocal microscopy showed minimal penetration of Adh/Adhno and deep penetration of Inf/Infno into the erosive lesions. The groups Hel, Adh, Inf, Tetno, and Infno resulted in the formation of a layer of material over the enamel, which was effective in inhibiting the progression of erosion. In conclusion, the infiltrant, with or without etching, was able to penetrate and protect the enamel against dental erosion. The other resin-based materials, except for the two-step conventional adhesive, were able to penetrate and inhibit the progression of erosive lesions only when they were applied after enamel etching.

scholarly journals The effect of a bleaching agent on the pigment removal and the bond strength of bulk-fill resin composites: an in vitro study

2018 ◽  
Vol 47 (5) ◽  
pp. 298-304
Author(s):  
Caroline de Farias CHARAMBA ◽  
Renally Bezerra Wanderley LIMA ◽  
Sônia Saeger MEIRELES ◽  
Rosângela Marques DUARTE ◽  
Ana Karina Maciel ANDRADE
Keyword(s):  
Bond Strength ◽  
Color Change ◽  
Resin Composite ◽  
Distilled Water ◽  
Filling Material ◽  
Resin Composites ◽  
Bleaching Agent ◽  
Microtensile Testing ◽  
Before And After

Abstract Introduction In order to simplify the technique, reduce the time required for direct adhesive restorations, bulk-fill resin composites have been developed be applied in bulk placement up to 4 mm increments. Objective Evaluating the color change (ΔE) and the microtensile bond strength (μTBS) of bulk fill resin composites (BFRC) to dentin after the immersion in regular beverages and the application of bleaching systems. Method Forty-five human molar teeth were randomly distributed in three groups according to the filling material (n=15): Filtek Bulk Fill, Tetric N Ceram Bulk Fill and Filtek Z100. The restored teeth were immersed in coffee, wine and distilled water (n=5) for 72 hours. The color parameters were measured using a spectrophotometer, having as basis the CIE L*a*b*, before and after tooth staining and application of bleaching processes. Subsequently, the teeth were sectioned to obtain the specimens for the microtensile testing. The ΔE values were analyzed applying the Kruskal-Wallis and the Wilcoxon tests, and the μTBS values were analyzed applying the ANOVA and the Bonferroni tests (p<0.05). Result There were no significant differences in the ∆E values when comparing the BFRCs to the conventional resin composite (CRC) in most of the experimental groups. The ∆E values did not present significant differences before and after the application of bleaching processes for all the tested resin composites. The BFRCs presented higher μTBS values than the CRC after exposure to distilled water, wine and bleaching agent. Conclusion The studied BFRCs presented similar color stability to the CRC. The BFRCs presented higher bond strength to the dentin than the CTC in most of the evaluated conditions. The bleaching agent was not effective in whitening the stained restored teeth.

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