In Vitro Effect of Innovative Desensitizing Agents on Dentin Tubule Occlusion and Erosive Wear

2019 ◽  
Vol 44 (2) ◽  
pp. 168-177 ◽  
Author(s):  
SA Garofalo ◽  
LO Sakae ◽  
AC Machado ◽  
SR Cunha ◽  
DM Zezell ◽  
...  
Keyword(s):  
Erosive Wear ◽  
Repeated Measure ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Surface Loss ◽  
Dentin Tubule ◽  
Desensitizing Agents ◽  
Dentin Tubules ◽  
Vitro Effect ◽  
Tubule Occlusion

SUMMARY Purpose: The purpose of this study was to evaluate the effects of four in-office desensitizing products on dentin tubule occlusion and erosive wear. Methods: Dentin hypersensitivity was simulated by EDTA application for five minutes. The specimens were randomly allocated into five groups (n=11), according to treatment: No treatment - Control (C), Duraphat (DUR), Desensibilize Nano P (NP), ClinPro XT Varnish (XTV), and ClinPro White Varnish (CWV). They were then submitted to erosive/abrasive cycling for five days. After EDTA, treatment, and cycling, the specimens were analyzed with an environmental scanning electron microscope (ESEM) to verify the number of opened dentin tubules (ODT) which were counted by using ImageJ software, and with a profilometer to determine the surface curvature/loss. ESEM data were analyzed with two-way repeated measure analysis of variance and Tukey tests. For the profilometer, data were analyzed with Kruskal-Wallis, Tukey, and Mann-Whitney tests. Results: After treatment, all groups showed lower ODT than the control, without significant differences between them. After cycling, the only group that showed lower ODT than the control was group XTV; however, it did not significantly differ from the other groups. For the profilometric analysis, there were significant differences in SL between the experimental times after treatment and after cycling for all groups (p<0.05). After cycling, no surface loss was detected in groups DUR and XTV, which presented a significantly different curvature than group NP and the control group, but not from group CWV. Surface loss was detected for the control and groups NP and CWV, without difference among them. Conclusion: All desensitizing agents tested presented promising results concerning the obliteration of dentin tubules immediately after treatment. XTV was the only desensitizer capable of preventing the reopening of the tubules after the erosive/abrasive challenges. XTV and DUR presented a protective effect against dentin erosive wear.

Evaluation of Dentinal Changes Following Application of Three Different Desensitizing Agents

2017 ◽  
Vol 68 (7) ◽  
pp. 1573-1577 ◽  
Author(s):  
Simona Stoleriu ◽  
Galina Pancu ◽  
Angela Ghiorghe ◽  
Dorina Cerasella Sincar ◽  
Sorina Solomon ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Dentinal Tubule ◽  
Nacl Solution ◽  
Human Teeth ◽  
Desensitizing Agents ◽  
Strontium Acetate ◽  
Dentin Tubules ◽  
Tubule Occlusion ◽  
Scanning Electron

The aim of this in vitro study was to evaluate the effect of three desensitizing tooth pastes on the dentinal tubule occlusion. Thirty dentin discs having a thickness of 3 mm were obtained by cutting human teeth. The discs were submersed in citric acid for 30 seconds to open the dentin tubules. Then the discs were cut in two halves. In each group 10 halves were kept in 0.9% NaCl solution) and the other 10 halves were exposed to the action of one of the tested desensitizing toothpastes. The dentin samples were placed in the machine designed to simulate tooth brushing. Three commercial desensitizing toothpastes were chosen to be applied on dentin surface. The morphology of dentin samples and the level of tubule occlusion was scored using scanning electron microscope. All the three desensitizing toothpastes demonstrated significant effects on dentinal tubule occlusion. The tooth paste containing arginine and calcium carbonate as active ingredients showed the highest degree of tubule occlusion, followed by the dentifrice containing strontium acetate and sodium fluoride.

Evaluation of Dentin Tubule Sealing Rate Improved by Attaching Ultrathin Amorphous Calcium Phosphate Sheet

2014 ◽  
Vol 631 ◽  
pp. 258-261 ◽  
Author(s):  
Nobuhiro Kato ◽  
Arata Isai ◽  
Ei Yamamoto ◽  
Hiroaki Nishikawa ◽  
Masanobu Kusunoki ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Tooth Enamel ◽  
Dental Enamel ◽  
Gingival Recession ◽  
Dentin Hypersensitivity ◽  
Mechanical Stimuli ◽  
Dentin Tubule ◽  
Human Dentin ◽  
Dentin Tubules

Dentin hypersensitivity is induced by mechanical stimuli or heat stimuli applied via dentin tubules exposed by lost of dental enamel or gingival recession. Common treatments for dentin hypersensitivity are resin coating or laser irradiation. However, these treatments have some problems such as poor biocompatibility or insufficient durability. We have been developing a treatment that creates artificial tooth enamel by attaching flexible ultrathin calcium phosphate sheet having a crystal structure similar to that of tooth enamel to seal the dentin tubules. In this study, the quantitative evaluation of dentin tubule sealing rate improved by attaching ultrathin amorphous calcium phosphate (APC) sheet on human dentin is presented. The obtained sealing-rate by APC sheet application was 70.9 ± 4.8 %.

scholarly journals Effect of Erosion/Abrasion Challenge on the Dentin Tubule Occlusion Using Different Desensitizing Agents

2017 ◽  
Vol 28 (2) ◽  
pp. 216-224 ◽  
Author(s):  
Gabriela D. Canali ◽  
Rodrigo N. Rached ◽  
Rui F. Mazur ◽  
Evelise M. Souza
Keyword(s):  
Repeated Measurements ◽  
Dentinal Tubule ◽  
Third Molars ◽  
Citric Acid Solution ◽  
Healthy Human ◽  
Dentin Permeability ◽  
Dentin Tubule ◽  
Before And After ◽  
Desensitizing Agents ◽  
Tubule Occlusion

The aim of the study was to evaluate dentinal tubule occlusion, measuring the dentin permeability (Lp) and using different desensitizing agents before and after abrasive/erosive challenge. Dentin discs from 42 healthy human third molars were obtained. Minimum Lp was measured after a smear layer simulation using #600 SiC paper and maximum Lp after an immersion in 0.5 M EDTA. The specimens were treated with different desensitizers: two varnishes (Clinpro XT Varnish-CV, Fluor Protector-FP), a paste (Desensibilize Nano P-NP) and a gel (Oxa Gel-OG). The Lp of each specimen was measured immediately after the desensitizers’ application. The discs were subjected to erosion/abrasion cycles for 7 days, with 0.5% citric acid solution (6x/day) and tooth brushing (3x/day). Lp was measured after the first, fourth and seventh day of the challenge. The data were analyzed by 3-way ANOVA with repeated measurements and by a Games-Howell test (α=5%). FP and CV did not show significant differences in Lp immediately after application until the 7th day (p<0.05). OG showed a significant increase in Lp after the 4th and 7th days. NP resulted in a significantly higher permeability compared to the other materials immediately after the application and after the 1st day of challenge. All the desensitizers reduced the dentin permeability immediately after application. However, only the varnishes were able to maintain the occlusive effect after the erosion/abrasion challenge.

scholarly journals Comparitive Evaluation of Novel Desensitising Agents on Dentinal Tubule Occlusion-A Scanning Electron Microscopic Study

2020 ◽  
pp. 11-18
Author(s):  
Dr. Madhavi Ajit Shetty ◽  
Dr. Sharad Kokate ◽  
Dr. Vibha Hegde
Keyword(s):  
Control Group ◽  
Dentinal Tubule ◽  
Tetraacetic Acid ◽  
Electron Microscopic ◽  
Dentinal Tubules ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Desensitizing Agents ◽  
Dentinal Hypersensitivity ◽  
Tubule Occlusion

Aim: To comparatively evaluate the occlusion of dentinal tubules by four novel desensitizing agents for treatment of Dentinal Hypersensitivity. Materials and Methods: The Diode Laser and three commercially available Desensitizers the NovaminR group- (SHY-NMTM), Colgate Sensitive Pro-relief, Gluma were investigated in this study. 30 extracted mandibular molars were used (n=30). They were divided into 5 groups including the control group in which no treatment was done. Teeth were ground under water-cooled trimming wheel, to prepare flat dentin surfaces. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M Ethylene Diamine Tetraacetic Acid (pH 7.4) for two minutes (applied with a micro brush) and then rinsed with an air-water syringe for 30 seconds.

Deep and Compact Dentinal Tubule Occlusion via Biomimic Mineralization and Mineral Overgrowth

Nanoscale ◽  
2021 ◽  
Author(s):  
Zhuo Chen ◽  
Yuyan Duan ◽  
Songzhe Shan ◽  
Kaida Sun ◽  
Gang Wang ◽  
...  
Keyword(s):  
Dentin Hypersensitivity ◽  
Dentinal Tubule ◽  
Desensitizing Agents ◽  
External Stimuli ◽  
Tubule Occlusion

Dentinal tubule (DT) occlusion by desensitizing agents has been widely applied to inhibit the transmission of external stimuli that cause dentin hypersensitivity (DH). However, most desensitizing agents merely accomplish porous...

scholarly journals Effects of desensitizing dentifrices on dentin tubule occlusion and resistance to erosive challenges

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiaoyi Zhao ◽  
Lin Wang ◽  
Jie Pan ◽  
Hans Malmstrom ◽  
Yan-Fang Ren
Keyword(s):  
Cross Sections ◽  
Orange Juice ◽  
Laser Scanning ◽  
Artificial Saliva ◽  
Disk Surface ◽  
Dentin Tubule ◽  
Dentin Tubules ◽  
Casein Phosphopeptide ◽  
Polyvinyl Methyl Ether ◽  
Tubule Occlusion

Abstract Background Many studies have demonstrated efficacy of casein phosphopeptide (CPP) containing products for dentin tubule occlusion for treatment of dentin sensitivity, but their effectiveness under dynamic erosive challenges remains to be elucidated. The purpose of the present study was to investigate the effectiveness of a desensitizing dentifrice containing CPP in occluding dentin tubules and resisting erosive challenges in comparison to that containing polyvinyl methyl ether/maleic acid (PVM/MA) copolymers. Methods A total of 33 dentin discs were prepared from coronal sections of human third molars and divided into 3 groups: a toothpaste containing CPP; a toothpaste containing PVM/MA and submicron silica; and a regular toothpaste (Controls). A soft-bristle toothbrush was used to brush the dentin discs with the dentifrices for 45 strokes in 30 s at a force of approximately 200 g. The brushing cycle was repeated after immersion of the dentin discs in artificial saliva overnight. The dentin discs were then challenged in orange juice for 10 min in an incubator rocking at 120 rpm. Three fields were randomly selected on each dentin disk surface to assess dentin tubule occlusions after each brushing cycle and after orange juice challenge with a 3D laser scanning microscope. Specimen cross sections were examined with a scanning electron microscope equipped with energy dispersive spectroscopy (SEM/EDS). Results After the first and second cycles of brushing, dentin tubules were occluded on average by 56.3% and 85.7% in CPP group, 66.2% and 88.1% in PVM/MA group, and 0.0 and 13.0% in the controls, respectively. There were no statistically significant differences in dentin tubule occlusions between the CPP and PVM/MA groups after two cycles of brushing (p>0.05). After dynamic erosive challenges with orange juice, 20.3% of the dentin tubules in the CPP group, 79.1% in the PVM/MA group and none in the control remained occluded (P<0.05). SEM/EDS imaging showed that dentin tubules were blocked with plugs containing dentifrice substances in CPP and PVM/MA groups after treatments, but none in the controls. Conclusions Desensitizing dentifrices containing CPP or PVM/MA could effectively occlude dentin tubules after two cycles of brushing. PVM/MA in combination with submicron silicon dioxide exhibited stronger resistance to dynamic erosive challenges by acidic beverages. Inorganic fillers that can enter dentin tubules and resist erosive challenges may be key for desensitizing dentifrices.

Does soft tissue mobilization assist static stretching to improve hamstring flexibility? A randomized controlled trial

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Shibili Nuhmani
Keyword(s):  
Soft Tissue ◽  
Controlled Trial ◽  
Knee Extension ◽  
Repeated Measure ◽  
Control Group ◽  
Static Stretching ◽  
Significant Difference ◽  
Hamstring Flexibility ◽  
Soft Tissue Mobilization ◽  
Experimental Group

AbstractObjectivesObjective of the study is to investigate whether Soft tissue mobilization (STM) can assist with static stretching to improve hamstring flexibly.MethodsThe design of the study was repeated measure design. The study was conducted at the physical therapy laboratory of Jamia Hamdard University, New Delhi. Participants included 78 healthy males with hamstring tightness, randomly assigned to either the control group (static stretching) or the experimental group (STM and static stretching). The experimental group received five sets of four different STM techniques, followed by two sets of 30-s static stretches 3 days per week over the course of 12 weeks. The control group received 5 min of sham ultrasound with an inactive probe prior to static stretching. Active knee extension test (AKE) was the outcome measure.ResultsBoth groups showed significant improvement in AKE compared with the baseline measurements. With ingroup analysis showed a significant difference in AKE across all measured time periods (weeks 4, 8, and 12) with pre-test in both groups (p<0.05). No significant difference in AKE improvement was found between groups (p>0.05).ConclusionThe results of this study show that STM prior to static stretching does not significantly improve hamstring flexibility among healthy individuals. Although this study cannot be generalized, the results may be useful for evidence-based practice in the management of hamstring tightness.

scholarly journals The control of pain due to dentin hypersensitivity in individuals with molar–incisor hypomineralisation: a protocol for a randomised controlled clinical trial

BMJ Open ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. e044653
Author(s):  
Ana Paula Taboada Sobral ◽  
Elaine Marcilio Santos ◽  
Ana Cecilia Aranha ◽  
Paulo Vinícius Soares ◽  
Caroline Moraes Moriyama ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Tooth Enamel ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Dentin Hypersensitivity ◽  
Molar Incisor Hypomineralisation ◽  
Randomised Controlled Clinical Trial ◽  
Sensitive Teeth ◽  
Randomised Controlled ◽  
Incisor Hypomineralisation

IntroductionDentin hypersensitivity (DH) is defined as high sensitivity of the vital dentin when exposed to thermal, chemical or tactile stimuli. Two mechanisms are required for the occurrence of DH: (1) the dentin must be exposed and (2) the dentinal tubules must be open and connected to the pulp. Molar–incisor hypomineralisation (MIH) is a qualitative abnormality of a genetic origin that affects tooth enamel and, in most cases, is accompanied by DH. The control of tooth sensitivity is fundamental to the successful treatment of MIH. The aim of the proposed randomised, controlled, clinical trial is to evaluate the effectiveness of different protocols for the control of DH in patients with teeth affected by MIH.Methods and analysisOne hundred and forty patients who meet the inclusion criteria will be allocated to four groups. Group 1 will be the control group (placebo). In Group 2, sensitive teeth will be sealed with PermaSeal (Ultradent). In Group 3, sensitive teeth will receive low-level laser (LLL, AsGaAl) at a wavelength of 780 nm (Laser XT Therapy, DMC, São Carlos, Brazil). In Group 4, sensitive teeth will be treated with both LLL and PermaSeal (Ultradent). DH will be evaluated 15 min after the application of the treatments and the patients will be reevaluated 1 week, 1 month, 3 months and 6 months after the treatments. The primary outcome of this study is change in pain/sensitivity, when evaluated through a Visual Analogue Scale, to determine the effectiveness of the proposed treatments, as well as differences among the evaluation times for each proposed treatment.Ethics and disseminationThis protocol has been ethically approved by the local medical ethical committee (protocol number: 4.020.261). Results will be submitted to international peer-reviewed journals and presented at international conferences.Trial registration numberNCT04407702.

Clinical Evaluation of Three Desensitizing Agents in Relieving Dentin Hypersensitivity

10.2341/07-5 ◽  
2007 ◽  
Vol 32 (6) ◽  
pp. 544-548 ◽  
Author(s):  
T. Pamir ◽  
H. Dalgar ◽  
B. Onal
Keyword(s):  
Clinical Evaluation ◽  
Clinical Relevance ◽  
Dentin Hypersensitivity ◽  
Active Ingredients ◽  
Desensitizing Agents

Clinical Relevance Three desensitizing agents with different active ingredients exhibited similar effects in the treatment of dentin hypersensitivity by mechanical blockage.

Sign in / Sign up

Export Citation Format

Share Document