scholarly journals PENGUJIAN VALIDASI ANALISIS KADAR ANDROGRAFOLID SECARA KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DENGAN ELUASI GRADIEN TERHADAP EKSTRAK HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NESS)

2005 ◽  
Vol 11 (1) ◽  
pp. 73-76
Author(s):  
Toetik Aryani
Keyword(s):  
Hplc Analysis ◽  
Hplc Method ◽  
Andrographis Paniculata ◽  
Percent Accuracy

Andrographolide of Andrographis paniculata Nees have been isolated and determined Extraction was carried out by maceration with ethanol as solvent. The concentration of Andrographolide was determined by HPLC method. The eluation was carried by out gradiently using methanol: water as an eluent and UV spectrophotometer at maks 228 as detector. The result of HPLC analysis are selectivity more than 1.2–1.5, r was 0.9937, precision was KV less than 10 percent; accuracy more than 90 percent, DL was 0.075, QL was 0.50 and PW less than F table. Andrographolide content of Andrographis paniculata Nees from Banyuwangi was 6.25 percent, Kediri was 14.69 percent and Surabaya was 6.83 percent.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

scholarly journals Validation of Metamizole, Thiamin and Pyridoxine Simultaneous Analysis Methods in Tablet Preparations Using HPLC

2018 ◽  
Vol 1 (1) ◽  
pp. 19
Author(s):  
Vevi Maritha ◽  
Lukman Labasy
Keyword(s):  
Mobile Phase ◽  
Hplc Analysis ◽  
Sodium Sulfite ◽  
Hplc Method ◽  
Optimum Concentration ◽  
Diode Array ◽  
Good Precision ◽  
Chromatographic System ◽  
Chromatographic Condition ◽  
Hydrolysis Of

The aim of the present study was to develop and validate HPLC method for the simultaneous assay of metamizole, thiamine and pyridoxine in tablet. Metamizole is a substance that is easily hydrolyzed in the precence of water and oxygen. To inhibit the hydrolysis of metamizole during sample preparation prior to HPLC analysis, sodium sulfite is added and its optimum concentration was investigated. The chromatographic system includes a RP C8(2) column (150x4.6 mm, 5 µm particle size) in conjunction with Photo Diode Array (PDA) detector. The optimal chromatographic condition was obtained using a mobile phase consisting of phosphate buffer 35mM pH 3.0: methanol (80:20), flowrate 1.0 ml/min, and 10 µl injection volume. The metamizole, thiamine and pyridoxine were detected at 275 nm and 361 nm for cyanocobalamin. The Hydrolysis of metamizole was successfully inhibited by adding solution containing 1.5 mg/mL sodium sulfite to solvent and 0.5 mg/mL sodium sulfite to mobile phase. The validation results indicate a good specificity and a linear detector responses with r>0.999. The accuracy (% recovery) for metamizole, thiamine and piridoxine were 100.26%; 99.09%; and 100.03%, respectively. The method yields good precision with RSD of metamizole, thiamine and pyridoxine were 2.0912%; 1.4489%; and 0.8418% respectively. In the robustness study, the small changes of mobile phase pH yielded unsymmetrical peaks and lower resolution. The validated method was successfully applied for simultaneous assay of metamizole, thiamine and pyridoxine in tablet. Keywords: validation; metamizole; thiamine; pyridoxine; hydrolysis of metamizole; HPLC

Study of Histamine Detection using Liquid Chromatography and Gas Chromatography

2021 ◽  
Vol 16 ◽  
pp. 1-9
Author(s):  
Muhammad Abdurrahman Munir ◽  
Muhammad Mukram Mohamed Mackeen ◽  
Lee Yook Heng ◽  
Khairiah Haji Badri
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Food Industry ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Chromatography Analysis ◽  
Quantitation Limit ◽  
Satisfactory Recovery

Histamine is a heterocyclic amine shaped by decarboxylation of the histidine. It is a compound that lack chromophore and involatile. However, the detection of histamine is imperative due to the characteristic of histamine has given several disadvantages in food industry. This paper describes methods for histamine detection by employing high performance liquid chromatography and gas chromatography. The derivatization techniques required for both methods in order to increase the sensitivity of chromatography analysis. Two derivatizing agents were applied in this study such as 9-flourenilmethyl chloroformate (FMOC – Cl) for HPLC analysis whereas for GC analysis a N,O-bis (trimethylsilyl)acetamide (BSA) was used. Method validation was in accordance to Commission Decision 657/2002/CE. The validation of specificity, linearity, precision, accuracy, detection limit and quantitation limit results indicate that the methods were acceptable. The linear range for both methods were at 0.16 – 5.00 µg∙mL-1. The determination of histamine using GC showed the superiority of this instrument compared to HPLC. Method applicability was also checked on real sample namely mackerel in order to acquire a satisfactory recovery for both methods.

scholarly journals Identification of Cardioactive Leonurus and Leonotis Drugs by Quantitative HPLC Determination and HPTLC Detection of Phenolic Marker Constituents

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100
Author(s):  
Kenny Kuchta ◽  
Jutta Ortwein ◽  
İhsan Çaliş ◽  
Rainer B. Volk ◽  
Hans W. Rauwald
Keyword(s):  
Quality Control ◽  
Ferulic Acid ◽  
South African ◽  
Neurological Disorders ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Rp Hplc ◽  
Fruit Samples ◽  
Leonurus Cardiaca ◽  
Quantitative Hplc

Officinal European Leonurus cardiaca, East Asian L. japonicus, and South African Leonotis leonurus are traditionally used for cardiovascular, gynecological, and neurological disorders. Nevertheless, a phytochemical assessment as a basis for their quality control and comparison amongst them has not yet been reported up to now. Here, a novel RP-HPLC method is presented for quantifying twelve phenolics, lavandulifolioside, verbascoside, hyperoside, ferulic acid, isoquercitrin, rutoside, apigenin-7- O-D-glucoside, and quercitrin, as well as chlorogenic, caffeic, rosmarinic, and cichoric acids, in 18 herbal and fruit samples of the three species, as well as in a L. cardiaca refined extract. Only ferulic acid was found in every sample, whereas rosmarinic acid and apigenin-7- O-D-glucoside were not detected in any sample. Chlorogenic, caffeic, and cichoric acids and rutoside were detected in all three species. Lavandulifolioside and verbascoside, the dominant phenolics of L. cardiac, were not present in any sample of L. japonicus. Lavandulifolioside was found in this first ever HPLC analysis on phenolics of L. leonurus. Hyperoside was not found in L. cardiaca, but in both L. japonicus and L. leonurus, whereas isoquercitrin was detected in L. cardiaca and L. leonurus, but not in L. japonicus. This approach facilitates identification and quality control via HPLC/HPTLC fingerprints.

scholarly journals Identification and Quantification of Andrographolide from Andrographis paniculata (Burm. f.) Wall. ex Nees by RP-HPLC Method and Standardization of its Market Preparations

2015 ◽  
Vol 14 (1) ◽  
pp. 71-78 ◽  
Author(s):  
BK Sajeeb ◽  
Uttom Kumar ◽  
Shimul Halder ◽  
Sitesh C Bachar
Keyword(s):  
Quantitative Estimation ◽  
Reversed Phase ◽  
Hplc Method ◽  
Andrographis Paniculata ◽  
Plant Sample ◽  
Rp Hplc ◽  
Ft Ir ◽  
Therapeutic Activities ◽  
Identification And Quantification

Andrographis paniculata (Burm. f.) Wall. ex Nees, commonly known as Kalmegh, is widely used as antimalarial drug in herbal and traditional systems. Andrographolide is the major triterpenoid present in the plant and responsible for its therapeutic activities. The identification and quantification of andrographolide were ascertained by various spectrophotometric and chromatographic analyses. The plant sample was extracted with methanol. The amorphous residue obtained from extraction was analyzed for identification of andrographolide by chemical method, TLC, UV-Vis, FT-IR and LCMS/MS analyses. The quantitative estimation of andrographolide content in plant sample was carried out by simple reversed phase HPLC method with C18 column using a mixture of water and methanol (35:65) as mobile phase at a flow rate of 0.7 mL/min, and the estimated concentration level of andrographolide was found as 38.36±0.42 ?g/mL in the solution of amorphous residue (50 ?g/mL). After quantitative determination of andrographolide, standardization of its market preparations was accomplished using the same RP-HPLC method. The estimated % potencies of andrographolide compared to reference standard in six market preparations were found to be 97.56, 98.37, 98.21, 95.60, 98.91 and 96.40.Dhaka Univ. J. Pharm. Sci. 14(1): 71-78, 2015 (June)

Sephadex LH-20 Cleanup, High Pressure Liquid Chromatographic Assay, and Fluorescence Detection of Zearalenone in Animal Feeds

1980 ◽  
Vol 63 (3) ◽  
pp. 642-646
Author(s):  
Huguette Cohen ◽  
Michel R Lapointe
Keyword(s):  
High Pressure ◽  
Fluorescence Detection ◽  
Hplc Analysis ◽  
Hplc Method ◽  
Normal Phase ◽  
High Pressure Liquid Chromatographic ◽  
Animal Feeds ◽  
Silica Cartridge ◽  
Normal Phase Hplc ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method is described to determine zearalenone in animal feeds at levels as low as 0.01 ppm. Samples are extracted with chloroform-ethanol and initially purified using a SEP-PAK silica cartridge, followed by column chromatography using Sephadex LH-20. Separation by normal phase HPLC is followed by fluorescence detection. Recoveries at levels of 1.0–0.01 ppm averaged greater than 90%. Confirmation included HPLC analysis of the sample and a zearalenone standard, using 3 different excitation wavelengths, and comparison of fluorescence responses obtained. The method was successfully applied to the analysis of 1 corn and 3 cornmeal samples. Zearalenone was detected in all 4 samples at levels of 0.379–19.2 ppm.

scholarly journals The HPLC Analysis of Aflatoxins in Raw Peanuts with Sep-pak® Cleanup

1984 ◽  
Vol 11 (1) ◽  
pp. 21-23 ◽  
Author(s):  
W. Jeffrey Hurst ◽  
Kevin P. Snyder ◽  
Robert A. Martin
Keyword(s):  
Hplc Analysis ◽  
Analysis Data ◽  
Hplc Method ◽  
Aqueous Acetone ◽  
Accuracy And Precision ◽  
Excellent Accuracy

Abstract An HPLC method for the determination of aflatoxins is described. The aflatoxins are extracted with aqueous acetone and interfering compounds precipitated with CuCO3. After defatting, the aflatoxins are extracted into CH2Cl2 for cleanup with silica Sep-pak® which eliminates other interfering compounds. The resulting extract is then treated with TFA to form the hemiacetal derivatives prior to final HPLC analysis. Data indicated that the method exhibits excellent accuracy and precision. In addition, it is time and solvent conservative.

scholarly journals The Pharmacokinetic Drug-Drug Interactions of Andrographis paniculata and Ibuprofen in the Plasma of Healthy Oryctolagus cuniculus Rabbits

2020 ◽  
Vol 5 (2) ◽  
pp. 40
Author(s):  
Mutakin Mutakin ◽  
Sandra Megantara ◽  
Batari A. Larasati ◽  
Yogiyanto Yogiyanto ◽  
Jutti Levita ◽  
...  
Keyword(s):  
Oral Administration ◽  
Oryctolagus Cuniculus ◽  
Hplc Method ◽  
Andrographis Paniculata ◽  
Mobile Phase Flow Rate ◽  
Single Oral Administration ◽  
Group 3 ◽  
Group 2 ◽  
Pharmacokinetic Drug ◽  
Group 1

An HPLC method was developed and validated for the pharmacokinetic drug-drug interaction between Andrographis paniculata and ibuprofen in the plasma of Oryctolagus cuniculus rabbits after a single oral administration of the mixture. Nine healthy rabbits (6 males and 3 females, weight 1.68-2.42 kg) were acclimatized for 7 days and were randomly divided into 3 groups. At day-8th the rabbits were group (1) treated with a single oral administration of ibuprofen (dose of 28 mg/kg BW); group (2) treated with a single oral administration of Andrographis paniculata infusion (7.04 mL/kg BW); group (3) treated with a single oral administration of a mixture of Andrographis paniculata (7.04 mL/kg BW) infusion and ibuprofen (dose of 28 mg/kg BW). Plasma samples were prepared by collecting the blood from the marginal ear vein at 0, 30, 60, 90, and 120 minutes after the mixture administration, followed by centrifuging it for 30 minutes 3000 rpm. Chromatographic separation was performed on a LiChrosorb RP-18 with methanol and double-distilled water (70:30) as the mobile phase, flow rate 1 mL/minute. UV detection was set at 227 nm. The absorption and distribution of ibuprofen were fast (Tmax = 30 min; Cmax = 4.02962 mcg/mL), however, interestingly this drug could improve the absorption and distribution of andrographolide in Oryctolagus cuniculus rabbits

scholarly journals Stability-Indicating HPLC Method for isoflavones aglycones analysis from Trifolium pratense L. extract

2020 ◽  
Vol 4 (1) ◽  
pp. 28-38
Author(s):  
Simony Martiny ◽  
Mairique Waszczuk ◽  
Samuel Kaiser ◽  
Marina Cardoso Nemitz ◽  
Valquiria Linck Bassani
Keyword(s):  
Hplc Analysis ◽  
Trifolium Pratense ◽  
Degradation Products ◽  
Hplc Method ◽  
Alkaline Media ◽  
Biochanin A ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Stability Indicating Method ◽  
The Stability

The purpose of this study was to develop and validate a fast HPLC stability-indicating method for simultaneously quantifying the four main isoflavones in Trifolium pratense. Validation procedures followed the ICH requirements for complex matrices. The stability-indicating tests were performed by exposing the isoflavones to conditions of forced degradation and further analysis for verifying the formation of degradation products and their possible interferences in the HPLC analysis. The major isoflavones of Trifolium pratense proved to be stable against acid and oxidative media, thermodegradation, and photodegradation. However, they proved to be unstable in alkaline media, even for short periods of exposure like 2h. In this condition, in addition to the peaks corresponding to isoflavones, the HPLC analysis showed the presence of three additional peaks which were eluted at different retention times to the reference substances, without interfering in the quantification of the four analytes of interest, formononetin, biochanin A, daidzein and genistein. The method was validated following ICH guidelines showing to be specific, linear, precise, accurate, and robust.This first report concerning a stability-indicating method revealed that the proposed HPLC method reliably quantify the isoflavones and separate them from the degradation products in a short time of analysis.

scholarly journals HPLC ANALYSIS OF AMINO ACIDS CONTENT IN CRAMBE CORDIFOLIA AND CRAMBE KOKTEBELICA LEAVES

2021 ◽  
pp. 111-116
Author(s):  
LIUDMYLA SLOBODIANIUK ◽  
LILIIA BUDNIAK ◽  
SVITLANA MARCHYSHYN ◽  
OLHA SKRYNCHUK ◽  
VICTORIIA KUDRIA
Keyword(s):  
Experimental Data ◽  
Amino Acids ◽  
Glutamic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Hplc Analysis ◽  
Raw Materials ◽  
Hplc Method ◽  
Primary Metabolites ◽  
Digestible Amino Acids

Objective: The aim of our study was to establish the content of some primary metabolites, such as amino acids in Crambe cordifolia and Crambe koktebelica. The lack of experimental data induced us to determine these compounds. Methods: Crambe cordifolia and Crambe koktebelica leaves were selected as the objects of the study. The amino acids in the raw materials were determined by the HPLC method. Results: The results of the research revealed that the leaves of Crambe cordifolia and Crambe koktebelica contain fifteen and sixteen free amino acids respectively. Among the free amino acids L-histidine was presented in Crambe cordifolia leaves in the greatest amount, its content was 12.19 µg/mg. The content of free L-arginine, L-valine, L-phenylalanine, L-isoleucine was the greatest in Crambe koktebelica leaves, it was 2.23 µg/mg, 2.04 µg/mg, 1.74 µg/mg, 1.50 µg/mg respectively. The content of bound L-glutamic acid, Glycine, L-arginine, L-leucine was the highest in Crambe cordifolia and Crambe koktebelica leaves. Conclusion: The results of the study showed that Crambe cordifolia and Crambe koktebelica can be considered as a source of highly digestible amino acids that can be used to treat some diseases.

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