scholarly journals DAYA HAMBAT VIRGIN COCONUT OIL TERHADAP PERTUMBUHAN JAMUR CANDIDA ALBICANS YANG DIISOLASI DARI SAMPEL SWAB VAGINA

2017 ◽  
Vol 6 (2) ◽  
pp. 209 ◽  
Author(s):  
Burhannuddin Burhannuddin ◽  
I W. Karta ◽  
B. Tresnanda ◽  
I G. N. D. Putra ◽  
I P. A. Darmada ◽  
...  
Author(s):  
Hortense Gonsu Kamga ◽  
Sarah Riwom Essama ◽  
Yves Le Grand Napa Tchuedji ◽  
Maurice Boda ◽  
Stève Henri Voundi Olugu ◽  
...  

Aims: The aim of this study is to evaluate in vitro the antifungal activity of virgin coconut oil and the white palm kernel oil on the growth of six species of the genus Candida. Study Design: This study is an experimental study. Place and Duration of the Study: Department of Microbiology, Laboratory of Microbiology of the University of Yaoundé I. Laboratory of Bacteriology, Yaoundé University teaching Hospital between April to September 2017. Methodology: We included six species of Candida. The method of diffusion discs in agar medium was used for Sensitivity tests and macro-dilution in liquid medium was used for dilution. Results: Majority of the species tested were resistant to conventional antifungals used. Candida hoemulonii was the most sensitive species to virgin coconut oil with percentages of inhibitions higher than 50 % when the concentration of oil was only 3.125 mg/mL and a maximum inhibition percentage of 90.10 % when the concentration of oil was 100 mg/mL. Candida albicans, was the least susceptible species to virgin coconut oil with a maximum inhibition percentage of 59.85 % when the concentration of oil was 100 mg/mL.  Candida lipolytica was the most sensitive specie to white palm kernel oil with a maximum inhibition percentage of 90,26 % when the concentration of oil was 100 mg/mL and Candida parapsilosis was the least susceptible species to white palm kernel oil with a maximum inhibition percentage of 52,69 % at the same concentration. In addition, the white palm kernel oil which was more active (P˂0.05) than virgin coconut oil was introduced in the Sabouraud broth and the MIC and MFC obtained with Fluconazole was lower on Candida albicans and Candida parapsilosis compared to activity of Fluconazole without palm kernel oil in the medium. Nystatin showed also lower MIC and MFC values on Candida parapsilosis when palm kernel oil was present in the culture medium. Conclusion: These results prove that these oils can be used to develop antifungals drugs.


2021 ◽  
Vol 5 (2) ◽  
pp. 223-232
Author(s):  
Putri Zelfitri Zen ◽  
Desmiwarti Desmiwarti ◽  
Sumaryati Syukur

Introduction : According to WHO, the prevalence of reproductive health problems in women has reached 33% from all types of diseases. One of the most frequent reproductive health problems is fluor albus. The incidence of candidiasis in pregnant women has doubly increased, especially at third trimester, compared to women who were not pregnant. Candida should be considered a dangerous pathogen in early pregnancy.Objective : This study compared the number of colonies before and after the administration of virgin coconut oil (VCO) to address vaginal discharge caused by C. albicans infection in pregnant women.Material and methods : This is an experimental study on 36 pregnant women. Examination of fluor albus is done by applying vaginal swab before and after administration of 1 tablespoon of VCO orally three times a day dan 5cc of VCO was applied on the vagina and vulva for intervention group, meanwhile the control group was not given anything. Statistical analysis to test the significance is done by using T-test.Results : There was a difference on average number of colonies with higher number of colonies prior to administration (137.17 ± 16.35 vs 122.17 ± 14.42). The average number of colonies at the first examination was 96.06 ± 8.85 colonies and increased at the second examination to 112.89 ± 12.49 colonies.Conclusion : There was an effect of VCO administration on decreasing the number of colonies in the intervention group (p=0.000), while in control group, there was increasing average number of colonies in the second examination (p=0.000).Keywords: Candida albicans, fluor albus, pregnant women, virgin coconut oil


2021 ◽  
Vol 5 (2) ◽  
pp. 229
Author(s):  
SatyaTejaswi Akula ◽  
A Nagaraja ◽  
M Ravikanth ◽  
NGovind Raj Kumar ◽  
Y Kalyan ◽  
...  

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Nur Ainatul Mardia Bt Mohamad Nasir ◽  
Zurainie Bt Abllah ◽  
Intan Azura Bt Shahdan ◽  
Anil Azura Bt Jalaludin

Introduction: As time progressed, our diet changed to include the types of foods that will promote decay and so dental caries became a widespread and serious concern. Dental caries had been reported adding to the significantly high number of oral health problem worldwide, causing a burden to health care system. Despite the existing of prevention courses, the numbers did not appear to decrease. Originated from natural resources, virgin coconut oil (VCO) has been proposed as an alternative treatment as it has been reported to demonstrate antimicrobial activity on various bacteria. Hence, the present study aimed to investigate whether VCO has antimicrobial effects on certain, selected cariogenic bacteria. Materials and Methods: Crude extract of VCO was prepared by using a natural method of fermentation. The composition of VCO were screened using Gas Chromatography Mass Spectrophotometry (GCMS). The antimicrobial activities of VCO were evaluated against three selected oral pathogens; Streptococcus mutans, Lactobacillus casei and Candida albicans using disc diffusion method. The inhibitory activities of VCO were determined by measuring the diameter of inhibition zone. The antimicrobial activities were further tested using broth micro-dilution technique to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Results: The inhibition zone was observed on Candida albicans plate, however no inhibitions were observed on Streptococcus mutans and Lactobacillus casei plates. The MIC and MBC results were inconsistent in 3 duplication that have been done for all the bacteria. Conclusion(s): VCO demonstrated potential antimicrobial activity against Candida albicans but not on Streptococcus mutans and Lactbacillus casei.


2016 ◽  
Vol 28 (2) ◽  
Author(s):  
Lui Dwen Tjin ◽  
Ame Suciati Setiawan ◽  
Emma Rachmawati

Introduction: Virgin Coconut Oil (VCO) is an oil which is purely made as a product of the coconut palmand has been used for centuries in the culinary industry and the medical field. Currently, VCO is used widely in medical field due to its effectiveness as antibacterial, antiviral and antifungal properties. Objective of this experimental study was to prove the effectiveness of virgin coconut oil by determining its exposure time at concentration of 25% to inhibit growth of oral Candida albicans. Methode: The research was done by quasi experiment design using the serial dilution method. The exposure time test was performed using a spread plate technique with virgin coconut oil at concentration of 25 % against oral Candida albicans isolated from saliva. Intervals of day 0, 1, 2 and 3 were used. Result: shows that virgin coconut oil inhibited the growth of oral C. albicans cells at day 2. Conclusion: virgin coconut oil with concentration of 25% can inhibit the growth of oral C. albicans with the exposure time of 2 days, which is similar with other antifungals.


2018 ◽  
Vol 71 (3) ◽  
pp. 259-262
Author(s):  
Monica Monica ◽  
Eva Fauziah ◽  
Sarworini Bagio Budiardjo ◽  
Margaretha Suharsini ◽  
Heriandi Sutadi ◽  
...  

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Aulia Alfi

Virgin Coconut Oil (VCO) adalah bahan alami yang memiliki sifat antimikroba (antivirus, antibakteri, dan antijamur). Sehingga VCO dapat memberikan efek pengawet pada bahan makanan, salah satunya adalah roti manis. Penelitian ini dilakukan untuk mengevaluasi pengaruh VCO terhadap karakteristik (fisik dan kimia) dan umur simpan roti manis. Roti manis dianalisis secara fisik (tekstur dan porositas) dan kimia (kadar air, kadar abu, kadar lemak, kadar protein, dan kandungan karbohidrat), dan analisis umur simpan dengan FFA, uji organoleptik dan jamur setiap dua hari selama delapan hari penyimpanan di suhu ruang. Variasi perlakuan roti manis adalah dari rasio konsentrasi VCO: margarin: mentega, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). Hasil penelitian menunjukkan bahwa VCO tidak memiliki pengaruh yang signifikan terhadap karakteristik fisik dan karakteristik kimia roti manis. Namun, VCO berpengaruh signifikan terhadap kadar air roti manis yang dihasilkan, roti manis K memiliki kadar air tertinggi (22,36%) dan berbeda dengan sampel roti manis lainnya. VCO secara efektif menghambat pertumbuhan jamur di roti manis pada konsentrasi 8%, 12%, dan 16%. Roti manis K dan A memiliki masa simpan 4 hari, sedangkan roti manis B, C, dan D memiliki masa simpan 6 hari.Kata kunci: VCO, roti manis, karakteristik, umur simpanABSTRACTVirgin Coconut Oil (VCO) is a natural ingredient that has antimicrobial (antiviral, antibacterial, and antifungal) properties. So that VCO can provide a preservative effect on food ingredients, one of which is sweet bread. This research was conducted to evaluate the effect of VCO on characteristics (physical and chemical) and shelf life of sweet bread. Sweet bread was analyzed physically (texture and porosity) and chemistry (moisture content, ash content, fat content, protein content, and carbohydrate content), and shelf life analysis with FFA, organoleptic and mold tests every two days for eight days of storage at ambient temperature. Treatment variations of sweet breads is from the ratio of the concentration of VCO: margarine: butter, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). The results showed that VCO did not have a significant effect on the physical characteristics and chemical characteristics of sweet bread. However, the VCO has a significant effect on the water content of the sweet bread produced, sweet bread K has the highest moisture content (22,36%) and it is different from other sweet bread samples. VCO effectively inhibits the growth of sweet bread mold at concentrations of 8%, 12%, and 16%. K and A sweet bread has a shelf life of 4 days, while sweet breads B, C, and D have a shelf life of 6 days.Keywords: VCO, sweet bread, characteristics, shelf life


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