Antioxidant effect of humic acids on rat hepatocytes

The antioxidant properties of humic acids of various origins have been investigated (from bio-humus, black soil from Ternopil region, and peat from different regions, such as: Velykyi Liubin, Drohobych and Belarus Republic). Titrimetric analysis has been shown the quantitative composition of the acidic functional groups of the obtained humic acids. According to the data of thermogravimetric analysis, the values of parameters, showing the ratio of their aliphatic to the cyclic part were determined. It was found that all obtained humic acids are characterized by high antioxidant activity: the formation of thiobarbituractive products and carbonyl groups of proteins in liver hepatocytes were decreased. The inhibition of the products of free radical formation of lipid components and proteins was better inhibited by 1% solutions of humates from vermicompost and humic acids from peat. The results indicate prospects of the humic acids for creating the means of restoring the physiological functions of biological objects in pathological conditions under conditions of free radical stress for application in cosmetic pharmaceutical products.

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Antioxidant and Antiglycation Properties of Seventeen Fruit Teas Obtained from One Manufacturer

Applied Sciences ◽

10.3390/app10155195 ◽

2020 ◽

Vol 10 (15) ◽

pp. 5195 ◽

Cited By ~ 1

Author(s):

Izabela Zieniewska ◽

Anna Zalewska ◽

Małgorzata Żendzian-Piotrowska ◽

Jerzy Robert Ładny ◽

Mateusz Maciejczyk

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Aromatic Amino Acids ◽

Raw Material ◽

Quantitative Composition ◽

Production Location ◽

Dried Fruit ◽

Health Promoting ◽

High Antioxidant Activity

The antioxidant activity of teas depends on the type and quality of the ingredients used in the process of tea production, location of the crops, and manner of the raw material processing. Our study is the first to compare the antioxidant and antiglycation properties of seventeen fruit teas obtained from one manufacturer. We evaluated three different brewing times (3, 5, and 10 min) and two brewing temperatures (70 and 100 °C). We demonstrated that infusions with the longest brewing time reveal the highest antiradical activity, while increased brewing temperature does not significantly affect the assessed parameters. The best antioxidant properties were obtained for the teas made from lemon balm with pear, forest fruits, cranberry with pomegranate, raspberry, and raspberry with linden. Fruit teas owe their high antioxidant activity to the presence of polyphenolic compounds in infusions. Extracts from fruit teas also diminish the oxidation and glycation of albumin in vitro, observed as a decrease in the fluorescence of aromatic amino acids and advanced glycation (AGE) and oxidation (AOPP) protein products levels. In conclusion, in order to prepare fruit teas with the best antioxidant properties, a longer extraction time is needed. The health-promoting properties of dried fruit infusions can be modified by changing the qualitative and quantitative composition of the ingredients.

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Pyruvate reduces anoxic injury and free radical formation in perfused rat hepatocytes

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1996.270.3.g535 ◽

1996 ◽

Vol 270 (3) ◽

pp. G535-G540 ◽

Cited By ~ 3

Author(s):

A. B. Borle ◽

R. T. Stanko

Keyword(s):

Free Radical ◽

Protective Action ◽

Rat Hepatocytes ◽

Radical Formation ◽

Antimycin A ◽

Anoxic Injury ◽

H2o2 Generation ◽

H2o2 Formation ◽

Free Radical Formation ◽

Ldh Release

The effects of 5 mM pyruvate on anoxic injury, superoxide (O2-.) and hydrogen peroxide (H2O2) generation, and lactate dehydrogenase (LDH) release during reoxygenation after 2.5 h anoxia were studied in perfused rat hepatocytes. When pyruvate was present during anoxia and reoxygenation, there was little anoxic injury, and the generation of free radicals and LDH release during reoxygenation were reduced 50-60%. When Pyruvate was added during reoxygenation, there was no decrease in O2-. or LDH release, although H2O2 formation was depressed. Free radical formation and anoxic/reperfusion injury were significantly reduced when pyruvate was added during the anoxic period only. Pyruvate reduced the deleterious effects of 10 microM antimycin A by preventing the increase in O2-. formation and LDH release evoked by the inhibitor. These results indicate that pyruvate protected hepatocytes against anoxic injury and that its protective action occurred principally during anoxia and not during reoxygenation. Pyruvate appeared to act at a mitochondrial site, since it reduced the deleterious effects of antimycin A.

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Oxygen free radical formation by rat hepatocytes during postanoxic reoxygenation: scavenging effect of albumin

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1994.266.3.g451 ◽

1994 ◽

Vol 266 (3) ◽

pp. G451-G458 ◽

Cited By ~ 2

Author(s):

P. Caraceni ◽

A. Gasbarrini ◽

D. H. Van Thiel ◽

A. B. Borle

Keyword(s):

Free Radical ◽

Trypan Blue ◽

Cell Injury ◽

Rat Hepatocytes ◽

Radical Formation ◽

Low Level ◽

Short Period ◽

Reoxygenation Injury ◽

Free Radical Formation ◽

Ldh Release

Free radical formation and reoxygenation injury were studied in rat hepatocytes perfused with Krebs-Henseleit bicarbonate buffer containing 1% or no albumin. After 2, 2.5, or 3 h of anoxia followed by 1 h reoxygenation in the absence of albumin, free radical formation assessed by low-level chemiluminescence and cell injury measured by lactate dehydrogenase (LDH) release and by trypan blue uptake increased proportionately. Chemiluminescence increased 4- to 7-fold, LDH release and trypan blue uptake increased 1.5- to 2-fold, compared with the end of anoxia. With 1% albumin, there was no increase in free radical formation during reoxygenation, and LDH release returned to control levels. There was a linear relation between the increase in chemiluminescence and the rise in LDH release (r2 = 0.83) and the increase in trypan blue uptake (r2 = 0.80), suggesting that free radical formation during reoxygenation is responsible for the cell injury. These experiments demonstrate that freshly isolated hepatocytes produce oxygen free radicals detectable by low-level chemiluminescence and that reoxygenation injury occurs after a relatively short period of anoxia (2-3 h). Albumin acts as a free radical scavenger, suppresses the release of reactive oxygen species, and significantly reduces reoxygenation injury.

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Tanshinone (Salviae miltiorrhizae Extract) Preparations Attenuate Aminoglycoside-Induced Free Radical Formation In Vitro and Ototoxicity In Vivo

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.47.6.1836-1841.2003 ◽

2003 ◽

Vol 47 (6) ◽

pp. 1836-1841 ◽

Cited By ~ 89

Author(s):

Ai-Mei Wang ◽

Su-Hua Sha ◽

Wojciech Lesniak ◽

Jochen Schacht

Keyword(s):

Developing Countries ◽

Free Radical ◽

Antioxidant Properties ◽

Concurrent Treatment ◽

Traditional Medicines ◽

Salviae Miltiorrhizae ◽

Free Radical Formation ◽

Radical Generation

ABSTRACT Antioxidant therapy protects against aminoglycoside-induced ototoxicity in animal models. A clinically suitable antioxidant must not affect the therapeutic efficacy of aminoglycosides or exhibit any side effects of its own. In addition, the treatment should be inexpensive and convenient in order to be implemented in developing countries where the use of aminoglycosides is most common. Standardized Salviae miltiorrhizae extracts (Danshen) are used clinically in China and contain diterpene quinones and phenolic acids with antioxidant properties. We combined in vitro and in vivo approaches to investigate the effect of a clinically approved injectable Danshen solution on aminoglycoside-induced free radical generation and ototoxicity. In vitro, Danshen inhibited gentamicin-dependent lipid peroxidation (formation of conjugated dienes from arachidonic acid), as well as the gentamicin-catalyzed formation of superoxide (in a lucigenin-based chemiluminescence assay) and hydroxyl radicals (oxidation of N,N-dimethyl-p-nitrosoaniline). Danshen extracts were then administered to adult CBA mice receiving concurrent treatment with kanamycin (700 mg/kg of body weight twice daily for 15 days). Auditory threshold shifts induced by kanamycin (approximately 50 dB) were significantly attenuated. Danshen did not reduce the levels in serum or antibacterial efficacy of kanamycin. These results suggest that herbal medications may be a significantly underexplored source of antidotes for aminoglycoside ototoxicity. Such traditional medicines are widely used in many developing countries and could become an easily accepted and inexpensive protective therapy.

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DOXORUBICIN FREE RADICAL FORMATION AND LIPID PEROXIDATION IN RAT HEPATOCYTES

Journal of Pharmacy and Pharmacology ◽

10.1111/j.2042-7158.1985.tb14092.x ◽

1985 ◽

Vol 37 (S12) ◽

pp. 20P-20P

Author(s):

T.J. Lavelle ◽

J. Basra ◽

J.R. Brown ◽

L.H. Patterson

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Rat Hepatocytes ◽

Radical Formation ◽

Free Radical Formation

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Implication for Hydrogen Free Radical Formation in the Presence of Hydrogen Nanobubbles

10.1055/s-0040-1702122 ◽

2020 ◽

Author(s):

Ichiro Otsuka

Keyword(s):

Free Radical ◽

Radical Formation ◽

Free Radical Formation

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Flavones and Their Analogues as Bioactive Compounds – An Overview

Mini-Reviews in Organic Chemistry ◽

10.2174/1570193x15666180418154510 ◽

2019 ◽

Vol 16 (4) ◽

pp. 377-391 ◽

Cited By ~ 1

Author(s):

B.S. Jayashree ◽

H. Venkatachalam ◽

Sanchari Basu Mallik

Keyword(s):

Biological Activity ◽

Free Radical ◽

Bioactive Compounds ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Cost Effective ◽

Free Radical Scavenging ◽

Polyphenolic Compounds ◽

Pharmacological Activities ◽

Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

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