scholarly journals Production of calves following nonsurgical transfer of fresh and refrigerated bovine demi-embryos

1996 ◽  
Vol 5 (5) ◽  
pp. 521-527 ◽  
Author(s):  
Peter Bredbacka ◽  
Ülle Jaakma ◽  
Ilmar Müürsepp
Keyword(s):  
Pregnancy Rate ◽  
Room Temperature ◽  
Pregnancy Rates ◽  
Optimal Conditions ◽  
Simple Method ◽  
High Pregnancy Rate

The viability of microblade-bisected Day-7 embryos transferred to recipients without zona pellucidae directly after splitting or after overnight storage at 4°C was investigated in two experiments. In Experiment 1, 26 demi-embryos of excellent or good quality and two of fair quality were transferred to 28 heifer recipients 1-3 h after splitting. The transfers resulted in 20 pregnancies (71.4% pregnancy rate), and 19 calves were born (one pregnant heifer was slaughtered). Another 14 demi-embryos were cultured 1 to 3 h at room temperature, transported for 3 h at 8-10°C and stored for 12 h in a refigerator at 4°C, Twelve of the 14 demi-embryos were considered transferable after storage and nine of these were transferred to nine recipients, of which five became pregnant. Four live calves were born. In Experiment 2, 21 excellent or good quality demi-embryos were transferred into 21 heifer recipients to produce 12 pregnancies (57.1%) and 10 live calves. Another 19 demi-embryos (17 excellent to good quality and two fair quality) were transferred after storage for 1-2 h at 8°C and 18 h at 4°C. Five recipients became pregnant, of which four delivered live calves. It is concluded that a high pregnancy rate can be achieved after the transfer of fresh demi-embryos produced by a simple method of embryo bisection under farm conditions. Although the transfer of demi-embryos stored overnight at 4°C results in decreased pregnancy rates, refrigeration of demiembryos may be useful in certain practical situations. However, further experiments are needed to determine the optimal conditions for overnight storage of demi-embryos.

scholarly journals Association Among Spinnbarkeit, Electrical Conductivity, and Crystallization of Cervical Mucus and Pregnancy Rate in Egyptian Baladi Cows

2022 ◽  
Author(s):  
Mahmoud Yassin Mohamed ◽  
Ahmed Mohamed Abd El-Hafeez ◽  
Mohamed El-said Ibrahim ◽  
Mohamed Hussein Ramadan ◽  
Amin Mohamed Amin ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Pregnancy Rate ◽  
Steroid Hormones ◽  
Close Correlation ◽  
Pregnancy Rates ◽  
Cervical Mucus ◽  
Marginal Effect ◽  
High Pregnancy Rate ◽  
Biochemical Profiles ◽  
Mineral Levels

Abstract Alteration of the biophysical and biochemical characteristics of cervical mucus (CM) due to differences in steroid hormones through the estrus cycle leads to different pregnancy rates. This investigation aims to study the possible relationship between CM properties with biochemical profiles, macro-mineral levels, and steroid hormones concentrations, and their effects on pregnancy rates of Egyptian cows. Fourteen Baladi cows were used and synchronized. The model log-likelihood ratio was highly significant (P=0.0009), and reported that the spinnbarkeit (SPK), electrical conductivity (EC), and crystallization (CRS) had significant effect on high pregnancy rate. The 3rd level of SPK (>13.5 cm) and EC (>15 mS/cm) was the highest significant (P=0.0016 & 0.0517, respectively) and a clear positive of estimate marginal effect (20.2543 & 10.6192, respectively) attitude towards the pregnancy rate. However, in case of the CRS, the significant effect was in the first two levels (P=0.0321 & 0.0425, respectively) with a high pregnancy rate, reverse the last 2 levels. Total protein, cholesterol, glucose, potassium, chloride levels, and estradiol concentrations were observed higher with increasing levels of SPK and EC and appearance of typical fern patterns (first two levels of CRS), in contrast to sodium, and progesterone concentrations that decreased with elevating levels of SPK and EC and appearance of atypical fern patterns (last two levels of CRS). There was a close correlation between CM properties and steroid hormones (P4 & E2). So, alterations in CM properties, especially SPK, EC, and CRS, can be utilized to foresee estrus time and, as a result, insemination time.

A Simple Method for Estimation of Lipoxygenase and Cyclo-Oxygenase Pathways in Human Platelets - the Use of Thiobarbituric Acid Reaction

1980 ◽  
Vol 44 (02) ◽  
pp. 111-114 ◽  
Author(s):  
Hiroshi Takayama ◽  
Minoru Okuma ◽  
Haruto Uchino
Keyword(s):  
Time Course ◽  
Normal Values ◽  
Human Platelet ◽  
Thiobarbituric Acid ◽  
Human Platelets ◽  
Optimal Conditions ◽  
Experimental Conditions ◽  
Simple Method ◽  
Acid Reaction ◽  
Optimal Ph

SummaryTo develop a simple method for estimation of platelet lipoxygenase (PLO) and cyclo-oxygenase (PCO) pathways, the arachidonic acid (AA) metabolism of human platelet was investigated under various experimental conditions by the use of the thiobarbituric acid (TBA) reaction and a radioisotope technique. A TBA-reactive substance different from malondialdehyde (MDA) via PCO pathway was detected and shown to be derived from the PLO pathway. Since the optimal pH and time course of its formation were different from those of MDA formation via PCO pathway, PLO and PCO pathways were estimated by quantitating the TBA-reactive substances produced by the incubation of AA either with aspirin-treated platelets or with untreated ones, respectively, each under optimal conditions. Normal values expressed in terms of nmol MDA/108 platelets were 1.17±0.34 (M±SD, n = 31) and 0.79±0.15 (n = 31) for PLO and PCO pathways, respectively.

Hormonal stimulation and some risk factors studied to improve bovine pregnancy rate at the Coastal areas of Barisal district of Bangladesh

2018 ◽  
Vol 52 (1-4) ◽  
pp. 22-30
Author(s):  
A K Paul ◽  
S M T Rahman
Keyword(s):  
Pregnancy Rate ◽  
Coastal Region ◽  
Hormonal Treatment ◽  
Coastal Areas ◽  
Pregnancy Rates ◽  
Hormonal Stimulation ◽  
Crossbred Cows ◽  
Treatment Groups ◽  
History Of ◽  
Group A

Hormonal treatment of cows at the coastal region of Barisal district of Bangladesh was performed to assess the improvement of pregnancy rate. A total of 100 cows and heifers with irregular history of cyclicity were selected randomly and divided into five treatment groups. The groups were A (treated with anthelmintic), B (treated with anthelmintic, vitamin ADE and multivitamin powder), C (treated with PGF2α), D (treated with GnRH) and E (treated with GnRH and PGF2α). Each group comprised of 20 animals. The age, breed and parity of experimental cows were considered during treatment. In the study, the cows treated with both GnRH and PGF2α (group E) showed significantly (p<0.05) higher estrus (80%) and pregnancy rate (60%) than that of group A, B, C and D. The overall estrus rates of local and crossbred cows were 64% and 70%, respectively and the pregnancy rates were 40 and 52%, respectively. The crossbred cows responded significantly (p<0.05) to hormonal treatment than that of local cows. Parity-2 cows showed higher estrus sign than that of other parities. However, the pregnancy rates were higher significantly (p<0.05) in parity-2 and parity ≥4 cows than that of parity-0, parity-1 and parity-3 cows. The pregnancy rate was also found higher in case of 4 to <5 years old cows than that of 2 to <3, 3 to <4, 5 to <6, and ≥6 years old. It may conclude that the hormonal regimen increases the pregnancy rate as well as decreases the undesired waiting of estrus and conception. Further study with more sample size will reveal the more effective treatment for cows at the coastal areas of Bangladesh.

Hormonal stimulation and some risk factors studied to improve bovine pregnancy rate at the Coastal areas of Barisal district of Bangladesh

2018 ◽  
Vol 52 (1-4) ◽  
pp. 22-30
Author(s):  
A K Paul ◽  
S M T Rahman
Keyword(s):  
Pregnancy Rate ◽  
Coastal Region ◽  
Hormonal Treatment ◽  
Coastal Areas ◽  
Pregnancy Rates ◽  
Hormonal Stimulation ◽  
Crossbred Cows ◽  
Treatment Groups ◽  
History Of ◽  
Group A

Hormonal treatment of cows at the coastal region of Barisal district of Bangladesh was performed to assess the improvement of pregnancy rate. A total of 100 cows and heifers with irregular history of cyclicity were selected randomly and divided into five treatment groups. The groups were A (treated with anthelmintic), B (treated with anthelmintic, vitamin ADE and multivitamin powder), C (treated with PGF2α), D (treated with GnRH) and E (treated with GnRH and PGF2α). Each group comprised of 20 animals. The age, breed and parity of experimental cows were considered during treatment. In the study, the cows treated with both GnRH and PGF2α (group E) showed significantly (p<0.05) higher estrus (80%) and pregnancy rate (60%) than that of group A, B, C and D. The overall estrus rates of local and crossbred cows were 64% and 70%, respectively and the pregnancy rates were 40 and 52%, respectively. The crossbred cows responded significantly (p<0.05) to hormonal treatment than that of local cows. Parity-2 cows showed higher estrus sign than that of other parities. However, the pregnancy rates were higher significantly (p<0.05) in parity-2 and parity ≥4 cows than that of parity-0, parity-1 and parity-3 cows. The pregnancy rate was also found higher in case of 4 to <5 years old cows than that of 2 to <3, 3 to <4, 5 to <6, and ≥6 years old. It may conclude that the hormonal regimen increases the pregnancy rate as well as decreases the undesired waiting of estrus and conception. Further study with more sample size will reveal the more effective treatment for cows at the coastal areas of Bangladesh.

scholarly journals Synthesis and characterization of a new ZIF-67@MgAl2O4 nanocomposite and its adsorption behaviour

RSC Advances ◽  
2021 ◽  
Vol 11 (22) ◽  
pp. 13245-13255
Author(s):  
Mehdi Davoodi ◽  
Fatemeh Davar ◽  
Mohammad R. Rezayat ◽  
Mohammad T. Jafari ◽  
Mehdi Bazarganipour ◽  
...  
Keyword(s):  
Room Temperature ◽  
Magnesium Aluminate ◽  
Synthesis And Characterization ◽  
Magnesium Aluminate Spinel ◽  
Adsorption Behaviour ◽  
Zeolitic Imidazolate Framework ◽  
Simple Method

New nanocomposite of zeolitic imidazolate framework-67@magnesium aluminate spinel (ZIF-67@MgAl2O4) has been fabricated by a simple method at room temperature with different weight ratios.

scholarly journals EPR Spectra of Sintered Cd1−xCrxTe Powdered Crystals with Various Cr Content

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3449
Author(s):  
Ireneusz Stefaniuk ◽  
Werner Obermayr ◽  
Volodymyr D. Popovych ◽  
Bogumił Cieniek ◽  
Iwona Rogalska
Keyword(s):  
Curie Temperature ◽  
Room Temperature ◽  
Integral Intensity ◽  
Resonance Field ◽  
Epr Spectra ◽  
Paramagnetic Resonance ◽  
Simple Method ◽  
Paramagnetic Curie Temperature ◽  
Cr Content ◽  
Electron Paramagnetic

In this paper, we show a simple method of producing ferromagnetic materials with a Curie temperature above room temperature. The electron paramagnetic resonance (EPR) spectra of Cd1−xCrxTe (0.002 < x < 0.08) were measured with a dependence on temperature (82 K < T < 381 K). Obtained EPR lines were fitted to a Lorentz-shaped curve. The temperature dependencies of the parameters of the EPR lines, such as the peak-to-peak linewidth (Hpp), the intensity (A), as well as the resonance field (Hr), were studied. Ferromagnetism was noticed in samples at high temperatures (near room temperature). For a sample with a nominal concentration of chrome of x = 0.05, a very strong intrinsic magnetic field is observed. The value of the effective gyromagnetic factor for this sample is ge = 30 at T = 240 K. An increase of chrome concentration above x = 0.05 reduces the ferromagnetic properties considerably. Analysis of the temperature dependencies of the integral intensity of EPR spectra was carried out using the Curie–Weiss law and the paramagnetic Curie temperature was obtained.

P–131 Significance of the phenomenon of blastomere exclusion from compaction: Its relation to irregular cleavage, blastocyst development rate, and pregnancy rate

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Watanabe ◽  
M Tomida ◽  
S Suzuki ◽  
Y Matsuda ◽  
K Yoshikai ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Chromosome Analysis ◽  
Development Rate ◽  
Pregnancy Rates ◽  
Blastocyst Transfer ◽  
Blastocyst Development ◽  
Miscarriage Rate ◽  
Single Blastocyst Transfer ◽  
Significant Difference ◽  
Irregular Cleavage

Abstract Study question When does blastomere exclusion from compaction increase and what effect does it have on the embryo? Summary answer More blastomere were excluded from compaction in embryos with irregular cleavage, resulting in lower blastocyst development rates, but no decrease in pregnancy rates at transfer. What is known already It has been reported that many of the chromosome analysis results of blastomere excluded from compaction were aneuploid, and pointed out that this exclusion may be related to the repair of blastocyst euploidy, but the effect of the number of excluded blastomere has not been reported. Study design, size, duration This is a retrospective study of 578 embryos that developed into morula with time-lapse monitoring by EmbryoScope (Vitrolife) in 2018–2019. Participants/materials, setting, methods The target embryos were classified into two groups: embryos with normal first and second cleavage (normal cleavage group) and embryos with irregular cleavage (dynamics of one cell dividing into three or more cells), called “direct cleavage”, at either cleavage (DC group), and the number of blastomere excluded from compaction during morula formation was recorded and compared. The blastocyst development rate and single blastocyst transfer pregnancy rates of the two groups were compared. Main results and the role of chance There are 286 in the normal cleavage group and 292 in the DC group. The mean number of excluded blastomere was 0.76 and 3.55, respectively, which was significantly higher in the DC group (P &lt; 0.01). Good blastocyst (Gardner classification 4 or higher) development rate was 84.5% (239/283) and 65.8% (181/275), respectively, and high grade blastocyst (Gardner classification BB or higher) development rate was 43.9% (105/239) and 14.9% (27/181) of them, both significantly higher in the normal cleavage group (P &lt; 0.01). The single blastocyst transfer pregnancy rates were 31.6% (25/79) and 32.4% (11/34), and the miscarriage rates were 24.0% (6/25) and 27.3% (3/11), respectively, neither was there a significant difference between the two groups. So, direct cleavage increased the number of blastomere excluded from compaction, decreased the rate of morula to good blastocyst development and reduced blastocyst grade, but did not affect blastocyst transfer pregnancy rate and miscarriage rate. Limitations, reasons for caution Please note that all target embryos must have developed into morula or larger (embryos that did not develop into morula will not be included in the study). Wider implications of the findings: Severe chromosomal aberrant blastomeres formed by direct cleavage were excluded from compaction, and the blastocyst development rate decreased due to a decrease in the amount of viable cells, but it is suggested that this blastomere exclusion mechanism is not related to euploidy after blastocyst development. Trial registration number Not applicable

scholarly journals Calving sub-season and reproductive efficiency of beef cows

2010 ◽  
Vol 39 (10) ◽  
pp. 2182-2186
Author(s):  
Cristiane Reinher ◽  
Júlio Otávio Jardim Barcellos ◽  
Vanessa Peripolli ◽  
Ênio Rosa Prates ◽  
Maria Eugênia Andrighetto Canozzi
Keyword(s):  
Pregnancy Rate ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Reproductive Efficiency ◽  
Climate Variations ◽  
Southern Brazil ◽  
Chi Square ◽  
Chi Square Test

It was evaluated in this work the effect of calving sub-season on the pregnancy rate of 7,726 multiparous Hereford beef cows grazing on natural pastures in southern Brazil, from 1994 to 2007. Calving sub-season periods were divided in 20-day intervals from August 12th to 31st; from September 1st to 20th; from September 21st to October 10th; from October 11th to 31st; from November 1st to 20th. Calving in each sub-season and pregnancy rate (PR) were analyzed by the Chi-square test. Overall means of each sub-season were 92.7% (from August 12th to 31st), 90.6% (from September 1st to 20th), 82.1% (from September 21st to October 10th), 77.7% (from October 11th to 31st), and 70.6% (from November 1st to 20th). Calving sub-season significantly influenced pregnancy rate. Cows which calved in the initial sub-season showed higher pregnancy rates than cows which calved from the fourth calving sub-season. However this difference did not occur in 1999 and in 2007 since calving rates did not statistically differ among the five sub-seasons. In 1998 and 2004, calving rates were lower and the effects of calving sub-season on pregnancy rates were higher, possibly due to climate variations. In general, pregnancy rate decreases as calving occurs later in the year, particularly in years when the weather adversely affects pasture growth.

scholarly journals The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Pregnancy Rates ◽  
Dominant Follicle ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Follicle Diameter ◽  
Follicle Size ◽  
Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

scholarly journals Effect of progesterone, human chorionic gonadotropin and progesterone + human chorionic gonadotropin treatment on conception rates in repeat breeder cows

2020 ◽  
Vol 89 (4) ◽  
pp. 307-315
Author(s):  
Hasan Alkan ◽  
Huseyin Erdem
Keyword(s):  
Pregnancy Rate ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Group 4 ◽  
Prostaglandin F ◽  
Repeat Breeder ◽  
Group 1

The aim of this study was to investigate the effects of hormonal support on the pregnancy rate in repeat breeder cows. Prostaglandin F2α + Ovsynch oestrus synchronization protocol was applied to the cows. Following the fixed time insemination (day 0), the cows were divided into 4 groups. In Group 1 (n = 42), progesterone releasing intravaginal device (PRID) was placed vaginally at 84 h and removed on the 9th day after the artificial insemination. In Group 2 (n = 40), the cows were administered human chorionic gonadotropin (hCG) on the 7th day. Group 3 (n = 45) was applied a combination of progesterone and hCG. Group 4 (n = 42) was not given any treatment. Blood samples were collected from all cows 4 times on days 3.5, 7, 12, and 18 to evaluate progesterone concentrations. The pregnancy rates were 40.47%, 37.50%, 44.44%, and 30.95% in Group 1, 2, 3, and 4, respectively (P > 0.05). In addition, in cows with progesterone concentrations <2 ng/ml on day 3.5, the pregnancy rates were found to be lower than in the cows with progesterone concentrations >2 ng/ml in Group 4 (P < 0.05). Progesterone supplementation in cows with progesterone concentrations < 2 ng/ml appeared to increase pregnancy rates (P < 0.05) in Groups 1 and 3. As a result, post-insemination hormonal applications in the repeat breeder cows did not increase the pregnancy rate. However, it was concluded that determination of progesterone concentrations on day 3.5 following artificial insemination and then hormonal support in the cows with low concentrations would increased the pregnancy rate.

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