DO PROBIOTICS HAVE A FUTURE IN  NEONATOLOGY?

The issue of feasibility and effectiveness of probiotics use in newborns is still discussable. A position letter of the Committee on nutrition of the European Society for Pediatric Gastroenterology, Hepatology, and Nutrition (ESPGHAN) and the Working group of the ESPGHAN on probiotics and prebiotics issues was published in May 2020 in “Pediatric Gastroenterology and Nutrition” magazine as for the use of probiotics in premature newborns. It stated that over 10,000 premature newborns from all over the world had taken part in random controlled probiotics tests. It was proved that probiotics, in general, could decrease the level of necrotizing enterocolitis, sepsis, and mortality. But the question of choice of microorganism strains, dosing, and duration of medication course remains open. On the other hand, an increasing number of commercial products containing probiotics of non optimal quality are available. In addition, a large number of departments in the world regularly suggest probiotic supplements as a treatment standard despite the absence of any solid evidence. According to the data of the latest meta-analysis, effectiveness in decreasing mortality and incidence was found in the minority of investigated strains or combinations. In the position letter authors wanted to give advice which specific strains can be potentially used and which cannot. Moreover, the emphasis was placed on issues of safety of probiotic supplements for premature newborns. Guarantee of quality of probiotic product is deliverance of probiotic strains by transfer genes of resistance to antibiotics and the possibility of regular identification of probiotic sepsis. There is a conditional recommendation (with a low credibility of evidence) to provide eitherLactobacillus rhamnosus GG ATCC53103, or combination of Bifidobacterium infantis Bb-02, Bifidobacterium lactis Bb-12, and Streptococcus thermophilus TH-4 for decreasing a risk of the necrotizing enterocolitis development subject to all issues of safety.

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DO PROBIOTICS HAVE A FUTURE IN NEONATOLOGY? (ANALYSIS OF THE LATEST DATA. PART 3)

Neonatology surgery and perinatal medicine ◽

10.24061/2413-4260.xi.4.42.2021.8 ◽

2021 ◽

Vol 11 (4(42)) ◽

pp. 45-52

Author(s):

T. Znamenska ◽

O. Vorobiova

Keyword(s):

Working Group ◽

Premature Newborns ◽

Routes Of Administration ◽

Randomized Controlled Clinical Trials ◽

Treatment Standard ◽

Transfer Genes ◽

Probiotic Strains ◽

Probiotic Product ◽

Clinical Questions

The issue of feasibility and effectiveness of probiotics use in newborns is still discussable. A position letter of the Committee on nutrition of the European Society for Pediatric NutritionGastroenterology, Hepatology, and(ESPGHAN) and the Working group of the ESPGHAN on probiotics and prebiotics issues was published in May 2020 in “Pediatric Gastroenterology and Nutrition” magazine as for the use of probiotics in premature newborns. The third part of the literature continues with the position paper and the results of many randomized controlled clinical trials of probiotics. The article considers the answers to 6 clinical questions posed by the working group of the Committee to assess the feasibility of use, routes of administration, dosage and duration of use, as well as the effectiveness and quality of probiotic drugs. It was proved that probiotics, in general, could decrease the level of necrotising enterocolitis, sepsis, and mortality. On the other hand, an increasing number of commercial products containing probiotics of non optimal quality are available. In addition, a large number of departments in the world regularly suggest probiotic supplements as a treatment standard despite the absence of any solid evidence. Moreover, the emphasis was placed on issues of safety of probiotic supplements for premature newborns. Guarantee of quality of probiotic product is deliverance of probiotic strains by transfer genes of resistance to antibiotics, the ability to regularly detect sepsis while using probiotics.

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The Impact of Human Milk on Necrotizing Enterocolitis: A Systematic Review and Meta-Analysis

Nutrients ◽

10.3390/nu12051322 ◽

2020 ◽

Vol 12 (5) ◽

pp. 1322 ◽

Cited By ~ 2

Author(s):

Emma Altobelli ◽

Paolo Matteo Angeletti ◽

Alberto Verrotti ◽

Reimondo Petrocelli

Keyword(s):

Systematic Review ◽

Human Milk ◽

Risk Reduction ◽

Necrotizing Enterocolitis ◽

Premature Infants ◽

Meta Analysis ◽

Preterm Formula ◽

The World ◽

Milk Banks ◽

The Impact

Background. Premature infants receiving breastfeed have a lower incidence of NEC than those fed preterm formula. This study aimed: (1) to update a systematic review and meta-analyses to evaluate the relationship between feeding and necrotizing enterocolitis (NEC) in low weight premature infants; (2) to conduct meta-regression analyses by subgroups; (3) to describe geographical distribution of milk banks in the world. Methods. Papers included in the meta-analysis were updated as of June 2019. Relative risks were used as a measure of effect size. Random effect models were used to account for different sources of variation among studies. For milk banks, the data reviewed by the literature were integrated with the information collected from countries’ institutional sites and milk bank networks. Results. Thirty-two papers were included in meta-analysis: six randomized controlled trials (RCTs) and 26 observational studies (OS). The census has found 572 milk banks around in the world. Brazil has the most active milk banks. RCTs meta-analysis indicates a risk reduction of NEC using human milk respect to formula: Relative risk (RR) = 0.62 (0.42–0.93). Seven OS compared quantities lower than human milk or higher than the 50th quantile showing a risk reduction of NEC:RR = 0.51 (0.31–0.85); 3 OS that evaluated human milk versus mixed feeding showing that human milk has a protective role on the development of NEC:RR = 0.74 (0.63–0.91). Results of subgroups analysis show that the risk reduction is statistically significant only for studies in which premature infants are given both their own and donated breastmilk. Conclusions. The possibility of preserving human milk and promoting donations guarantees an improvement in the health of newborns.

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Immunomodulatory Effect of “Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy” to Cure or Prevent Hospital Acquired (Nosocomial) Infections due to Clostridium difficile (C. diff), other Pathogenic Bacteria, and Autoimmune Diseases

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2018.11.1.1 ◽

2018 ◽

Vol 11 (1) ◽

pp. 3937-3949

Author(s):

Malireddy S Reddy

Keyword(s):

Immune System ◽

Gastrointestinal Tract ◽

Pathogenic Bacteria ◽

Molecular Level ◽

Host Immune System ◽

Hospital Acquired Infections ◽

The World ◽

Probiotic Strains ◽

The Individual ◽

Hospital Acquired

The worldwide popularity of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy to treat or prevent the hospital acquired infections (nosocomial infections) arose a great interest in the medical community around the world (Reddy and Reddy, 2016; 2017). The following questions were raised on this subject: Does Multiple Mixed Strain Probiotics directly inhibit the pathogenic bacteria (C. diff) in the gastrointestinal tract or indirectly through modulation of the host immune system or both? To be more specific, what is the exact and/or hypothetical mechanism at molecular level behind the breakthrough discovery of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy? To answer these questions, the specific immunomodulation regulatory functions of the individual Probiotic strains (on host) have beenresearched, investigated andoutlined in this article. A detailed explanation(s) and hypotheses have been proposed outlining the possible cumulativedirect bacteriological and indirect immunomodulatory effects (at the molecular level) of the Multiple Mixed Strain Probiotics used in Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy to successfully treat C. diff infection. A detailed scientific and research attempts were made to correlate the Probiotic induced immune activities in relation to the reduction of the symptoms associated with the hospital acquired Clostridium difficile infection during and after the Multiple Mixed Strain Probioitc Therapy. Results of the clinical trials, microbiological tests on feces, and the clinical blood tests significantly revealed that the reasons for the success of Dr. Reddy’s Multiple Mixed Strain Probiotic Therapy are multifold. Presumably, it is predominantly due to the immunomodulatory effect they have exerted on the host immune system along with the direct inhibition of C. diff bacteria by multiple Probiotics, due to the production of bacteriocins, lactic acid and nutritional competency.In addition, the size of the individual cells of the Probiotic strains in the Multiple Mixed Strain Probiotics and their significant effect on immunomodulation has been thoroughly discussed. Results clearly proved that if Probiotics are absent in the GI tract during C. diff infection, the chances of patient survival is zero. This is because of the excess immune stimulation and incurable damage to the epithelial cell barrier of the gastrointestinal tract caused by C. diff bacteria. The results also revealed, without any doubt, as of to-datethe latest discovery of Dr. M.S. Reddy’s Multiple Mixed Strain Probiotic Therapy is the best way to cure the deadly hospital acquired infections affecting millions of people around the world, with high degree of mortality. This has been attested by several practicng medical professionals and scientists around the world (Reddy and Reddy, 2017).

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An Understanding of the Global Status of Major Bacterial Pathogens of Milk Concerning Bovine Mastitis: A Systematic Review and Meta-Analysis (Scientometrics)

Pathogens ◽

10.3390/pathogens10050545 ◽

2021 ◽

Vol 10 (5) ◽

pp. 545

Author(s):

Paramanandham Krishnamoorthy ◽

Kuralayanapalya P. Suresh ◽

Kavitha S. Jayamma ◽

Bibek R. Shome ◽

Sharanagouda S. Patil ◽

...

Keyword(s):

Escherichia Coli ◽

Management Practices ◽

Meta Analysis ◽

Bacterial Species ◽

Clinical Mastitis ◽

Therapeutic Interventions ◽

Coagulase Negative Staphylococcus ◽

Prevalence Studies ◽

The World ◽

Pathogen Prevalence

In this study, the major mastitis pathogen prevalence in the cattle and buffalo of the world was estimated by a meta-analysis. Staphylococcus (S) species, Streptococcus (St) species, and Escherichia coli (Ec) prevalence studies reported during 1979–2019 were collected using online databases, and offline resources. A meta-analysis of these data was done with the meta package in R-Software. The Staphylococcus aureus was the major mastitis pathogen, mostly causing subclinical mastitis, Ec causing clinical mastitis and St causing subclinical and clinical mastitis. The pooled prevalence estimates of S, St, and Ec were 28%, 12%, and 11% in the world from 156, 129, and 92 studies, respectively. The S, St, and Ec prevalences were high in Latin America (51%), Oceania (25%), and Oceania (28%), respectively. Higher S, St, and Ec prevalences were observed by molecular methods, signifying high sensitivity and usefulness for future studies. Among bacterial species, S. aureus (25%) followed by coagulase-negative Staphylococcus species (20%), Escherichia coli (11%), St. agalactiae (9%), St. uberis (9%) were the important pathogens present in the milk of the world. We hypothesize that there is a urgent need to reduce mastitis pathogen prevalence by ensuring scientific farm management practices, proper feeding, therapeutic interventions to augment profits in dairying, and improving animal and human health.

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Meta-analysis reveals severe pollen limitation for the flowering plants growing in East Himalaya-Hengduan Mountains region

BMC Ecology ◽

10.1186/s12898-020-00322-6 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xianfeng Jiang ◽

Yanping Xie

Keyword(s):

Confidence Interval ◽

Pollen Limitation ◽

Meta Analysis ◽

Flowering Plants ◽

Biodiversity Hotspot ◽

Clear Relationship ◽

Hengduan Mountains ◽

Independent Data ◽

The World ◽

Global Biodiversity

Abstract Background Pollen limitation occurs widely and has an important effect on flowering plants. The East Himalaya-Hengduan Mountains region is a global biodiversity hotspot. However, to our knowledge, no study has synthetically assessed the degree of pollen limitation in this area. The present study aims to reveal the degree of pollen limitation for the flowering plants growing on East Himalaya-Hengduan Mountains and to test whether the reproductive features or the elevation is closely correlated with the degree of pollen limitation in this area. Results We complied data from 76 studies, which included 96 species and 108 independent data records. We found that the flowering plants in this area undergo severe pollen limitation [overall Hedges’ d = 2.004, with a 95% confidence interval (1.3264, 2.6743)] that is much higher than that of the flowering plants growing in many other regions around the world. The degree of pollen limitation was tested to determine the correlation with the capacity for autonomous self-reproduction and with the pollination pattern (generalized vs. specialized pollination) of plants. In addition, we found a clear relationship between elevation and the degree of pollen limitation, which indicates that plants might undergo more severe pollen limitation in relatively high places. Conclusions This paper is the first to address the severe pollen limitation of the flowering plants growing in East Himalaya-Hengduan Mountains region. Moreover, we reveal the positive correlation between elevation and the degree of pollen limitation.

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