Antimicrobial Effects of Some Plant Extracts on the Oral Pathogens Around the Removable Denture

2017 ◽  
Vol 1 (10) ◽  
Author(s):  
Hakan Demir ◽  
Tuğba Demir
Keyword(s):  
Plant Extracts ◽  
Antimicrobial Effects ◽  
Removable Denture ◽  
Oral Pathogens

Antimicrobial effects of plant extracts against Clostridium perfringens with respect to food-relevant influencing factors

2021 ◽  
Author(s):  
Ulrike Friedlein ◽  
Samart Dorn-In ◽  
Karin Schwaiger
Keyword(s):  
Influencing Factors ◽  
Plant Extract ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Screening Method ◽  
Extrinsic Factors ◽  
Antimicrobial Effects ◽  
Extract Concentration ◽  
Microdilution Method

The application of plant extracts (PEs) could be a promising option to satisfy consumers’ demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing factors. Due to the high opacity of PEs, resazurin was used as an indicator for bacterial growth instead of pellet formation. The highest value of the minimum inhibitory concentration (MIC) of the replications of each PE was defined as effective plant extract concentration (EPC), whereas the next concentration beneath the lowest MIC value was defined as the ineffective plant extract concentration (IEPC). The EPC of seven PEs: allspice, cardamom, cinnamon, clove, coriander, ginger and mace were between 0.625 - 10 g/kg, whereas extracts of caravey, nutmeg and thyme showed no antimicrobial activity up to the maximum concentration tested (10 g/kg) against C. perfringens in vitro. Two intrinsic factors, sodium chloride and sodium nitrite, displayed either synergistic/additive effects or no interaction with most PEs. By combination with PEs at its ineffective plant concentration (IEPC, 0.08 – 1.25 g/kg), MIC of NaCl and NaNO2 decreased from 25 – 50 g/kg to 6 – 25 g/kg and > 200 mg/kg to 0.2 – 100 mg/kg respectively. On the contrary, lipid (sun flower oil) at a low concentration inhibited the antimicrobial effects of all tested PEs. For extrinsic factors, only allspice, ginger and coriander could maintain their antimicrobial effects after being heated to 78 °C for 30 min. The synergistic effect between PEs and pH values (5.0 and 5.5) was also found for all PEs. The established screening method with resazurin and defining EPC and IEPC values allows the verification of antimicrobial effects of PEs under various food-relevant influencing factors in a fast and reproducible way.

scholarly journals KR-12-a5 is a non-cytotoxic agent with potent antimicrobial effects against oral pathogens

Biofouling ◽  
2017 ◽  
Vol 33 (10) ◽  
pp. 807-818 ◽  
Author(s):  
Karina Sampaio Caiaffa ◽  
Loiane Massunari ◽  
Marcelle Danelon ◽  
Gabriel Flores Abuna ◽  
Telma Blanca Lombardo Bedran ◽  
...  
Keyword(s):  
Cytotoxic Agent ◽  
Antimicrobial Effects ◽  
Oral Pathogens

scholarly journals The Isolation of Antimicrobial Resistant Aeromonas spp. from Aquaculture Samples and their Susceptibility to Medicinal Plant Extracts

2020 ◽  
pp. 56-67
Author(s):  
C. I. Chikwendu ◽  
R. K. Obi ◽  
K. C. Ibe ◽  
J. C. Orji
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Vernonia Amygdalina ◽  
Garcinia Kola ◽  
Antimicrobial Effects ◽  
Ocimum Gratissimum ◽  
Ethanol Extracts ◽  
Conventional Antibiotics ◽  
Aquaculture Water

Aeromonas spp, ubiquitous in both terrestrial and aquatic environments are becoming renowned as enteric pathogens of serious public health concern as they have a number of virulence and resistant determinants that are linked to both human and aquatic diseases due to consistent and incorrect use of antibiotics in aquaculture. The effect of crude aqueous and ethanol extracts of some medicinal plants on antimicrobial resistant Aeromonas spp. isolated from aquaculture water and fish samples was studied. Two hundred and forty (240) Aeromonas isolates, made up of 168 Aeromonas hydrophila and 72 Aeromonas salmonicida, were recovered from aquaculture water and fish gill samples collected from different commercial fish ponds using selective media. The isolates were assessed for their antibiotic susceptibility against ten (10) conventional antibiotics using the Kirby-Bauer technique. Extracts from three medicinal plants, Vernonia amygdalina, Ocimum gratissimum and Garcinia kola were also analyzed for their antimicrobial effects on the isolates that were resistant to the conventional antibiotics. Aeromonas hydrophila isolates expressed the highest resistant rates of 100%, 78.6% and 70.8% to aztreonam, cefotaxime and neomysin respectively, and the A. salmonicida isolates also had a similar trend of high resistant rates of 100%, 87.5% and 77.8% to aztreonam, neomysin and cefotaxime respectively. Antimicrobial resistant analyses with the plant extracts showed 100% inhibition of the isolates at 100 mg/ml for both aqueous and ethanol extracts. Phytochemical screening identified the presence of certain phytochemicals like alkaloids, saponins, tannins, flavonoids, steroids and glycosides which could have accounted for the antimicrobial effects of the plant extracts under study. It can be inferred then, that extracts from Vernonia amygdalina, Ocimum gratissimum and Garcinia kola can inhibit resistant aquaculture Aeromonas isolates and so can present an alternative source of antimicrobials in the effort to combat the increasing incidence of antimicrobial resistance in aquaculture.

scholarly journals Azadirachta indica Reduces Black Sigatoka in East African Highland Banana by Direct Antimicrobial Effects against Mycosphaerella fijiensis without Inducing Resistance

2017 ◽  
Vol 9 (4) ◽  
pp. 61 ◽  
Author(s):  
Alfred Kumakech ◽  
Hans. J. L. Jørgensen ◽  
David B. Collinge ◽  
Richard Edema ◽  
Patrick Okori
Keyword(s):  
Plant Extracts ◽  
Azadirachta Indica ◽  
Plant Pathogens ◽  
Antimicrobial Activities ◽  
Late Time ◽  
Black Sigatoka ◽  
East African ◽  
Pr Genes ◽  
Antimicrobial Effects ◽  
And Control

Black Sigatoka is a major disease of East African highland cooking bananas in Uganda. Aqueous extracts of Azadirachta indica, Cinnamomum zeylanicum and Capsicum annuum have shown the potential to reduce Black Sigatoka in banana plantlets. The mechanisms by which plant extracts confer protection against plant pathogens has previously been reported to involve activation of defence and direct antimicrobial activity. In the current study, both antimicrobial activities of selected extracts were studied as well as expression of three defence-related genes using quantitative real-time PCR. Gene expression was compared in susceptible (cv. Musakala, genomic group AAA-EA) and resistant (cv. Kayinja, genomic group ABB) banana cultivars. Additionally, Musakala treated with A indica extract at 1 day before inoculation (DBI) was tested for induction of defence-related genes at 0, 10 and 20 days after inoculation (DAI). Pathogenesis-related genes (PR-1 and PR-3) and non-expressor of PR-genes (NPR1B) were up-regulated in the resistant cultivar. The genes analysed responded at late time points to M. fijiensis inoculation in both extract-treated and control plants in the susceptible cv. Musakala. On the other hand, A. indica and C. annuum completely inhibited mycelial growth of M. fijiensis at 30% (w/v). These findings suggest that the effect of plant extracts on Black Sigatoka is strongly associated with the direct antimicrobial effects.

Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds

2000 ◽  
Vol 56 (1) ◽  
pp. 3-12 ◽  
Author(s):  
Jussi-Pekka Rauha ◽  
Susanna Remes ◽  
Marina Heinonen ◽  
Anu Hopia ◽  
Marja Kähkönen ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Extracts ◽  
Antimicrobial Effects

scholarly journals A comparative study to evaluate the effects of antibiotics, plant extracts and fluoride-based toothpaste on the oral pathogens isolated from patients with gum diseases in Pakistan

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Hassan ◽  
F. Shafique ◽  
H. Bhutta ◽  
K. Haq ◽  
T. Almansouri ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Bacterial Resistance ◽  
Curcuma Longa ◽  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Daily Routine ◽  
Significant Activity ◽  
Proteus Vulgaris ◽  
Medicinal Plant Extracts ◽  
Oral Pathogens

Abstract Oral diseases caused by various microorganisms are common around the world. Scientific research has now been focusing on novel medicines to overcome bacterial resistance and antibiotics side effects; therefore, the current study was designed to assess the efficacy of certain antibiotics, toothpaste, and medicinal plant extracts (Ajuga bracteosa and Curcuma longa) versus the bacterial pathogens isolated from the human oral cavity. A total of 130 samples were collected from Khyber Teaching Hospital Peshawar, Pakistan, among those 27 species isolated, and eight bacterial species were identified from the samples. Among all the bacterial species, Staphylococcus aureus (29.62%) and Proteus mirabilis (22.2%) were found to be more prevalent oral pathogens. In comparison, the least pervasive microbes were Proteus vulgaris, Shigella sonnei, Escherichia coli and Aeromonas hydrophila. The study also suggested that dental problems were more prevalent in males (41-50 years of age) than females. Among the eight antibiotics used in the study, the most promising results were shown by Foxicillin against A. hydrophila. The survey of TP1 revealed that it showed more potent antagonist activity against Proteus vulgaris as compared TP2 and TP3 that might be due to the high content of fluoride. The Curcuma longa showed more significant activity than Ajuga bracteosa (Stem, leaves and root) extracts. The data obtained through this study revealed that antibiotics were more effective for oral bacterial pathogens than toothpaste and plant extracts which showed moderate and low activity, respectively. Therefore, it is suggested that the active compounds in individual medicinal plants like Curcuma longa and Ajuga bracteosa could replace the antibiotics when used in daily routine as tooth cleansers or mouth rinses.

scholarly journals Comparative Evaluation of the Antimicrobial Effects of Different Mouthrinses against Oral Pathogens: An In Vitro Study1

2020 ◽  
Vol 21 (5) ◽  
pp. 500-508
Author(s):  
Eman Abu-obaid ◽  
Fouad Salama ◽  
Ala’a Abu-obaid ◽  
Fars Alanazi ◽  
Mounir Salem ◽  
...  
Keyword(s):  
Comparative Evaluation ◽  
Antimicrobial Effects ◽  
Oral Pathogens
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