scholarly journals Modulating the Haematological and Cytogenetic Effects of Mitomycin C by Aqueous Extract of Nut Grass (Cyperus rotundus L.)

2007 ◽  
Vol 1 (1) ◽  
pp. 52-54
Author(s):  
Ali H. Ad'hiah ◽  
Mohammed M. F. Al-Halbosiy ◽  
Rakad M. Al-Jumaily
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Mitomycin C ◽  
Aqueous Extract ◽  
Leucocyte Count ◽  
Bone Marrow Cells ◽  
Post Treatment ◽  
Cyperus Rotundus ◽  
Micronucleus Formation ◽  
Pre Treatment

The aqueous extract (5, 10 and 15 mg/kg) of nut grass (Cyperus rotundus L.) rhizomes was evaluated orally in albino male mice using some haematological (total leucocyte count) and cytogenetic (mitotic index, micronucleus formation and chromosomal aberrations of bone marrow cells) parameters. The extract interaction with the mutagen mitomycin C (MMC) was also evaluated through two types of treatments (pre- and post-treatments). The results revealed that the dose 15 mg/kg of the extract significantly increased the total count of leucocytes (7634.4 vs. 6783.3 cells/cu.mm. blood), while the mitotic index showed no significant differences, as compared to negative controls. However, the spontaneous formation of micronuclei in the bone marrow cells was significantly decreased in the three investigated doses of the extract (0.30, 0.32 and 0.29, respectively vs. 0.62%), while the chromosomal assay showed similar frequencies in the negative control and nut grass-treated animals. With respect to the interaction with MMC, the pre-treatment (15 mg/kg) enhanced the leucocyte count (10358.6 vs. 3800.2 cells/cu.mm.blood) and mitotic index (11.9 vs. 6.5%), and a similar picture was drawn when the pos-treatment was considered (8884.2 vs. 4292.7 cells/cu.mm.blood; 14.6 vs. 7.6%). However, the doses 5 and 10 mg/kg of the plant extract were much more effective in reducing the MMC-induced micronucleus formation in both types of treatments especially the dose 5 mg/kg (pre-treatment: 4.24 vs. 16.29%; post-treatment: 3.79 vs. 14.34%). With respect to chromosomal aberration assay, the dose 15 mg/kg of the extract was the most effective dose in reducing the MMC-induced aberrations, but the post-treatment was better than pre-treatment in this respect (0.29 vs. 0.79 aberration/cell).

scholarly journals Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice

2018 ◽  
Vol 12 (1) ◽  
pp. 124-131
Author(s):  
Ruqaya M. Ibrahim
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Post Treatment ◽  
Rosemary Extract ◽  
Mutagenic Action ◽  
Male Mice ◽  
Micronucleus Formation ◽  
Marrow Cells

This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center \Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg  in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.

scholarly journals Modulating the Mutagenic Effects of Mitomycin C by Aqueous Extract of Lentil (Lens culinars)

2009 ◽  
Vol 3 (1) ◽  
pp. 37-43
Author(s):  
M.M.F. Al-Halbosiy
Keyword(s):  
Mitotic Index ◽  
Chromosomal Aberration ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Leukocyte Count ◽  
Drug Application ◽  
Mutagenic Effect ◽  
Total Leukocyte Count ◽  
Micronucleus Formation ◽  
Mutagenic Effects

The present study aimed to evaluate the toxic and mutagenic effect of Lentil (Lens culinars) seed aqueous extract (0.5 , 1.0 and 1.5 mg/kg) and its ability to modulate the mutagenic effects of mytomycin C, using the male albino mice for hematological and cytogenetic analyses (total leukocyte count and mitotic index, chromosomal aberration and micronucleus formation of bone marrow cells). The evaluations were carried out through three type of treatment. In the first treatment the extract was dosed alone to the animals, while in the second and third treatment, interaction between the extract and mytomycin C (pre and post treatment) were carried out for such evaluations. The study showed that there was no toxic and mutational effects of the used extract .The result also showed that the dose 1.5mg/kg was significantly better than the other doses in all tests that were carried. The result also showed that there was aninhibition effect of the extract in relation to the mutational effects of mytomycin C. through its effects in increasing the total leukocyte count and mitotic index and reducing in chromosomal aberration, micronucleus formation before and after the drug application.

scholarly journals Effect of sage (Salvia officinalis) aqueous extract on mitotic index in albino male mice

2010 ◽  
Vol 4 (1) ◽  
pp. 36-43
Author(s):  
Roqaya, M. Al-Ezzy ◽  
Khulood Al-Samarrae ◽  
Ali H. Ad'haih
Keyword(s):  
Bone Marrow ◽  
Mitotic Index ◽  
Aqueous Extract ◽  
Bone Marrow Cells ◽  
Negative Control ◽  
Salvia Officinalis ◽  
Male Mice ◽  
Negative Controls ◽  
The Ideal ◽  
Marrow Cells

This study aimed to evaluate the effect of aqueous extract of sage (Salvia officinalis) on bone marrow cells in albino male mice by using three doses ( 83.9, 167.8 or 251.7 mg/kg) and cytosar drug at dose of ( 1.54 mg/kg). The results showed that sage has the ability to increase the mitotic index in mice in comparing with the negative control and in mice treated with cytosar drug that caused reduction in mitotic index. The results of pre- and post-treatment with the ideal dose of aqueous extract of sage and cytosar drug showed the ability of sage to increase the mitotic index of bone marrow cells in mice in comparing with the negative controls.

Phenotypic correction of Fanconi anemia group C knockout mice

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 700-704 ◽  
Author(s):  
Kimberly A. Gush ◽  
Kai-Ling Fu ◽  
Markus Grompe ◽  
Christopher E. Walsh
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Mitomycin C ◽  
Fanconi Anemia ◽  
Bone Marrow Cells ◽  
Bone Marrow Failure ◽  
Genetic Disorder ◽  
Hematopoietic Stem ◽  
Marrow Cells

Fanconi anemia (FA) is a genetic disorder characterized by bone marrow failure, congenital anomalies, and a predisposition to malignancy. FA cells demonstrate hypersensitivity to DNA cross-linking agents, such as mitomycin C (MMC). Mice with a targeted disruption of the FANCC gene (fancc −/− nullizygous mice) exhibit many of the characteristic features of FA and provide a valuable tool for testing novel therapeutic strategies. We have exploited the inherent hypersensitivity offancc −/− hematopoietic cells to assay for phenotypic correction following transfer of the FANCC complementary DNA (cDNA) into bone marrow cells. Murine fancc −/− bone marrow cells were transduced with the use of retrovirus carrying the humanfancc cDNA and injected into lethally irradiated recipients. Mitomycin C (MMC) dosing, known to induce pancytopenia, was used to challenge the transplanted animals. Phenotypic correction was determined by assessment of peripheral blood counts. Mice that received cells transduced with virus carrying the wild-type gene maintained normal blood counts following MMC administration. All nullizygous control animals receiving MMC exhibited pancytopenia shortly before death. Clonogenic assay and polymerase chain reaction analysis confirmed gene transfer of progenitor cells. These results indicate that selective pressure promotes in vivo enrichment offancc-transduced hematopoietic stem/progenitor cells. In addition, MMC resistance coupled with detection of the transgene in secondary recipients suggests transduction and phenotypic correction of long-term repopulating stem cells.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF OXALIS DEBILIS KUNTH AGAINST CCL4 - INDUCED LIVER DAMAGE

2017 ◽  
Vol 10 (9) ◽  
pp. 231
Author(s):  
Rojini Athokpam ◽  
Meenakshi Bawari ◽  
Manabendra Dutta Choudhury
Keyword(s):  
Aqueous Extract ◽  
Liver Damage ◽  
Elevated Serum ◽  
Treated Group ◽  
Hepatoprotective Activity ◽  
Post Treatment ◽  
Histopathological Study ◽  
Phytochemical Analysis ◽  
Ccl4 Intoxication ◽  
Pre Treatment

  Objective: To evaluate the hepatoprotective activity of aqueous extract of Oxalis debilis Kunth in carbon tetrachloride (CCl4)-induced hepatotoxicity in Swiss albino mice.Methods: Hepatotoxicity was induced by CCl4 30% in olive oil (1 ml/kg intraperitoneally). Mice were treated with aqueous extract of O. debilis at doses of 250 and 500 mg/kg body weight orally for 14 days. There were two groups, pre-treatment (once daily for 14 days before CCl4 intoxication) and post-treatment (2, 6, 24, and 48 hrs after CCl4 intoxication). The observed effects were compared with a known hepatoprotective agent, silymarin.Results: Pre-treatment and post-treatment groups of aqueous extract of O. debilis significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl4-treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of phenolic compounds, tannins, flavonoids, and saponins.Conclusion: The results of this study suggest that O. debilis can be used as safe, cheap, and alternative preventive and protective drugs against liver injury. The protective effect observed could be attributed to the presence of various phytochemicals which are responsible for the restoration of liver damage.

Short-Term Foamyviral Transduction with Virions Encoding Recombinant Corrects the Mitomycin C Hypersensitivity of Fancc −/− Progenitor Cells In Vitro and Restores Long Term Repopulating Activity of Fancc −/− Stem Cells In Vivo.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3171-3171
Author(s):  
Yue Si ◽  
Cordula Leurs ◽  
Edward Srour ◽  
Samantha Ciccone ◽  
Helmut Hanenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Mitomycin C ◽  
Bone Marrow Cells ◽  
Genetic Disorder ◽  
Foamy Virus ◽  
Alkylating Agents

Abstract Fanconi anemia (FA) is a complex autosomal recessive genetic disorder characterized within the hematological system by progressive bone marrow aplasia, a high propensity to develop acute myeloid leukemia, and hypersensitivity to alkylating agents including mitomycin c. The identification of individual FA genes raises the potential of using gene transfer technology to express/introduce the functional cDNA in/into deficient autologous stem cells. We have previously shown that in the absence of genetic correction with a retroviral mediated Fancc transgene, ex vivo culture of Fancc−/− stem/progenitor cells (HSPC) predisposes uncorrected Fancc−/− HSPC cells to clonal hematopoiesis (Haneline, Blood 2003). Therefore we examined the potential of a helper-free human foamy virus (HFV) derived construct that encodes both the human FANCC and EGFP transgenes to transduce murine Fancc−/− HSC in the absence of prestimulation. In initial experiments, we determined that 40–80% of progenitors were transduced following a single overnight HFV infection using a 20:1 moiety of infection. Subsequent studies demonstrated that HFV efficiently transduced primitive hematopoietic progenitors in G0 and G1 phases of the cell cycle as evidenced both by using multicolor fluorescence activated cell sorting and subsequent culture of sorted cell populations in high proliferating potential (HPP-CFC) and low proliferating potential colony forming assays. Aliquots of HFV transduced cells that were transduced with the construct encoding both Fancc and EGFP, or the reporter transgene only were transplanted into irradiated recipient mice. Four months following transplantation, bone marrow cells were isolated from the reconstituted recipients and clonogenic assays were established in a range of mitomycin c (MMC) concentrations. In these experiments, the MMC hypersensitivity of Fancc−/− progenitors was corrected to wild-type levels. To assess quantitatively the potential of HFV expressed FANCC to correct stem cell repopulating ability, we next utilized the competitive repopulating assay. In two replicate experiments, we determined that the repopulating activity of HFV-transduced Fancc−/− stem cells was comparable to wildtype controls six months following transplantation in primary and secondary recipients. Collectively, these data provide in vivo evidence that the HFV vector is an efficient vehicle for introducing a functional hFANCC transgene into quiescent Fancc−/− HSC in the absence of prestimulation and for complementing the cellular FA defect in vitro and in vivo.

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