scholarly journals Hemipteran Hopper Species Associated with Acid Lime Plants (Citrus aurantifolia L .) in the Sultanate of Oman: Candidate Vectors of Witches’-Broom Disease of Lime

2007 ◽  
Vol 12 ◽  
pp. 53 ◽  
Author(s):  
Syed Ali Razvi ◽  
Rashid Al-Shidi ◽  
Najma Mahmood Al-Zadjali ◽  
Yousuf Mohammad Al-Raeesy
Keyword(s):  
Relative Humidity ◽  
Disease Incidence ◽  
Sultanate Of Oman ◽  
Citrus Aurantifolia ◽  
Vector Population ◽  
Broom Disease ◽  
Acid Lime ◽  
Two Phases ◽  
One Year ◽  
Hishimonus Phycitis

Regular monitoring of hemipteran hopper species (including psyllids) associated with small-fruited acid lime trees (Citrus aurantifolia L.) was conducted for four years using motorized insect suction to determine the possible vectors of witches’- broom disease of lime (WBDL). The study was done in two phases: monitoring was done in Habra village, Wilayat Wadi AlMaawal (Batinah region) for one year from June 2000 to May 2001; then monitoring was done in Maharah village, Wilayat AlMusannah (Batinah region), for a period of three years from May 2001 to April 2004. Twelve species of cicadellid leafhoppers and one delpahacid planthopper species were collected, while no psyllids were found. Hishimonus phycitis (Distant) (Cicadellidae) was the most abundant hopper (78.4 % of collected individuals). Next in abundance were Toya sp. (Delphacidae), Circulifera haematiceps? and a deltocephalin leafhopper, respectively constituting 10.4, 3.8 and 2.4 % of the total catches of the four years. Nine other species made up 5% of the total collection: Exitianus nanus (Distant), Cicadulina sp. (either chinai (Ghauri) or storeyi (China)), Emposca distinguenda (Paoli), Amrasca biguttula (Ishihara), Deltocephalus (Recilia) pruthii (Metcalf), Neolimnus aegyptiacus (Mutsumura) and three undetermined species (one Deltocephalinae, one Typhlocybinae and one undeterminable to subfamily). Catches of H. phycitis were highest from November to March and lowest from May to September. There was a significant linear relationship between number of H. phycitis and maximum and mininmum temperature. Relative humidity was not significantly correlated to number of H. phycitis. In Maharah, young lime trees were free from WBDL but the disease incidence increased with age. H. phycitis is the best candidate vector of WBDL. The potential of Toya sp., Circulifera haematiceps? and an undetermined deltocephalin as candidate vectors is discussed. Finally, it is suggested that regular sprayings of acid lime trees with effective systemic insecticides during November to March each year can greatly reduce the vector population and can prevent or delay the spread of the disease to a great extent.

scholarly journals First Report of a 16SrXIV Group Phytoplasma Associated With Witches’-Broom Disease of Acid Lime (Citrus aurantifolia) in India

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 831-831 ◽  
Author(s):  
D. K. Ghosh ◽  
S. Bhose ◽  
P. Sharma ◽  
A. Warghane ◽  
M. Motghare ◽  
...  
Keyword(s):  
Citrus Aurantifolia ◽  
First Report ◽  
Broom Disease ◽  
Acid Lime

scholarly journals Occurrence of Witches'-Broom, a New Phytoplasma Disease of Acid Lime (Citrus aurantifolia) in India

Plant Disease ◽  
1999 ◽  
Vol 83 (3) ◽  
pp. 302-302 ◽  
Author(s):  
D. K. Ghosh ◽  
A. K. Das ◽  
Shyam Singh ◽  
S. J. Singh ◽  
Y. S. Ahlawat
Keyword(s):  
Uranyl Acetate ◽  
Citrus Aurantifolia ◽  
Rangpur Lime ◽  
Cuscuta Reflexa ◽  
Rough Lemon ◽  
Nagpur District ◽  
Citrus Production ◽  
Broom Disease ◽  
Acid Lime ◽  
Major Acid

In India, acid lime (Citrus aurantifolia (L.) Swingle) is one of the most important citrus fruits grown. It constitutes nearly 20% of the total citrus production. During 1995, an unusual type of disease was observed on a 6-year-old acid lime plant in an orchard in the Nagpur District in eastern Maharashtra. It was named witches'-broom disease (WBD) to reflect the most conspicuous symptom. Other symptoms included small chlorotic leaves, highly proliferated shoots, and shortened internodes. Leaves dropped prematurely and infected twigs were distorted. In advanced stages, infected branches had dieback symptoms. WBD of lime has been reported from Oman and UAE (1) and the causal phytoplasma was designated “Candidatus Phytoplasma aurantifolia” (2). Subsequent surveys in 1995-1998 revealed disease incidences as high as 5% in Maharashtra and in other major acid-lime-growing states—Andhra Pradesh, Tamilnadu, and Karnataka. After the grafting of infected acid lime shoots, disease symptoms developed on Troyer citrange, rough lemon, and Rangpur lime, but not on sweet orange (mosambi), mandarin (Nagpur), or trifoliate orange. The WBD agent was transmitted from infected acid lime to periwinkle (Catharanthus roseus) plants and vice versa by dodder (Cuscuta reflexa). Ultrathin sections of leaf midrib of infected acid lime plants were fixed on copper grids, stained with uranyl acetate and lead acetate, and examined in a JEM 100S transmission electron microscope. Numerous bodies having the characteristic morphology of phytoplasmas were observed in phloem sieve tubes of acid lime in diseased but not in healthy leaves. The phytoplasmal bodies ranged from 100 to 800 nm in diameter and were bounded by a poorly defined membrance. Freehand transverse sections of young internode regions of a WBD-infected periwinkle plant were stained in DAPI (4′, 6 diamidino-2-phenylindole; 1.0 μg/ml) and were observed with a fluorescent microscope (Leica). An intense bluish-white fluorescence in the phloem elements of diseased periwinkle and its absence in healthy samples were consistent with the presence of phytoplasmas. This is the first report of phytoplasma-induced witches'-broom disease of acid lime in India. References: (1) M. Garnier et al. Plant Dis. 75:546, 1991. (2) L. Zreik et al. Int. J. Syst. Bacteriol. 45:449,1995.

Expression of phytoplasma-induced witches’ broom disease symptoms in acid lime (Citrus aurantifolia) trees is affected by climatic conditions

2017 ◽  
Vol 66 (8) ◽  
pp. 1380-1388 ◽  
Author(s):  
A. G. Al-Ghaithi ◽  
A. M. Al-Sadi ◽  
M. S. Al-Hammadi ◽  
R. M. Al-Shariqi ◽  
R. A. Al-Yahyai ◽  
...  
Keyword(s):  
Climatic Conditions ◽  
Disease Symptoms ◽  
Citrus Aurantifolia ◽  
Broom Disease ◽  
Acid Lime

BIOASSAY TO DETERMINE CHERRY ROOTSTOCK TOLERANCE TO `BACTERIAL CANKER' CAUSED BY PSEUDOMONAS SYRINGAE PV SYRINGAE

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1354F-1355
Author(s):  
Elzbieta Krzesinska ◽  
Anita Nina Miller
Keyword(s):  
Relative Humidity ◽  
Pseudomonas Syringae ◽  
Bacterial Suspension ◽  
Avirulent Strain ◽  
Bacterial Canker ◽  
Symptom Development ◽  
Virulent Strains ◽  
Callus Production ◽  
Root Stock ◽  
One Year

An excised twig assay was developed to evaluate cherry geno-types for their tolerance to Pseudomonas syringae pv. syringae. One-year-old wood was collected at monthly intervals from October until January of `Royal Ann', `Corum', and a number of cherry rootstock. The rootstock included; F/12–1 and Giessen (GI) and M × M selections. A 2-cm incision (“^”-shaped flap) was made on each twig. A 20-μl droplet of inoculum or water was placed onto each incision. The inoculum was prepared with one avirulent (K4) and three virulent strains (W4N54, AP2, B15) concentrations (105, 106, or 107 cfu). Inoculated twigs were placed in test tubes and incubated at 15C in high relative humidity for 3 weeks. After incubation, twigs were evaluated for gummosis production (0–3, 0 = no gummosis), incision browning (1–4, 1 = yellow pith), and callus production (0–1, 0 = no callus). The concentration of bacterial suspension had no effect on symptom development. No gummosis or browning was observed on twigs inoculated with water or the avirulent strain. Based on the gummosis and browning ratings, rootstock M × M 2, M × M 39, M × M 60, GI 148-1, GI 154-2, and GI 154-4 were found to be resistant to these three strains of P. syringae in this assay. Root-stock F 12-1, GI 169–15, GI 172–9, and GI 173-9 were found to be tolerant.

scholarly journals Increasing enrolment in a national VA transitions of care programme: a pre–post evaluation of a data dashboard and nudge-based intervention

2021 ◽  
Vol 28 (1) ◽  
pp. e100416
Author(s):  
Brigid Connelly ◽  
Chelsea Leonard ◽  
David Gaskin ◽  
Theodore Warsavage ◽  
Heather Gilmartin
Keyword(s):  
Performance Metrics ◽  
Transitions Of Care ◽  
Audit And Feedback ◽  
Veterans Health ◽  
Health Administration ◽  
Care Programme ◽  
Post Evaluation ◽  
The Difference ◽  
Two Phases ◽  
One Year

BackgroundThe rural transitions nurse programme (TNP) is a care coordination intervention for high-risk veterans. An interactive dashboard was used to provide real-time performance metrics to sites as an audit and feedback tool. One-year post implementation, enrolment goals were not met. Nudge emails were introduced to increase TNP veteran enrolment. This study evaluated whether veteran enrolment increased when feedback occurred through a dashboard plus weekly nudge email versus dashboard alone.Setting/populationThis observational study included veterans who were hospitalised and discharged from four Veterans Health Administration hospitals participating in TNP.MethodsVeteran enrolment counts between the dashboard phase and dashboard plus weekly nudge email phase were compared. Nudge emails included run charts of enrolment data. The difference of means for weekly enrolment between the two phases were calculated. After 3 months of nudge emails, a survey assessing TNP transitions nurse and physician champion perceptions of the nudge emails was distributed.ResultsThe average enrolment for the four TNP sites during the ~20-month dashboard only phase was 4.23 veterans/week. The average during the 3-month dashboard plus nudge email phase was 4.21 veterans/week. The difference in means was −0.03 (p=0.73). Adjusting for time trends had no further effect. Four nurses responded to the survey. Two nurses reported neutral and two reported positive perceptions of the nudge emails.ConclusionDrawing attention to metrics, through nudge emails, maintained, but did not increase TNP veteran discharges compared to dashboard feedback alone.

scholarly journals Reproductive and cost assessment of a seasonal breeding program with Bos indicus in tropical Mexico

2021 ◽  
Vol 26 (2) ◽  
pp. e2130
Author(s):  
José Francisco Martínez-Albarrán ◽  
Carlos Salvador Galina-Hidalgo ◽  
Ivette Rubio-Gutiérrez ◽  
Wendy Leticia Balam-Villarreal ◽  
Manuel D. Corro-Morales
Keyword(s):  
Pregnancy Rate ◽  
Breeding Season ◽  
Phase 1 ◽  
Bos Indicus ◽  
Fixed Time ◽  
Breeding Program ◽  
Tropical Region ◽  
Two Phases ◽  
One Year ◽  
The Cost

Objective. To compare the reproductive performance of postpartum and open Bos indicus cows and to study the cost effectiveness of retaining non-pregnant animals after a short breeding season in tropical region of Mexico. Material and Methods. A total of 128 Bos indicus were included, 87 postpartum cows (PP) with ≤90 days after calving and 41 open cows (OC) with >90 days open. The study was divided into three phases: 1) Estrus synchronization followed by FTAI (day 0-10), 2) Estrus detection and AI (day 11-45) and 3) Natural mating (day 46-90). For the first phase, all animals were synchronized and AI at fixed time (day 10). Cows displaying overt signs of estrus (day 11-45) were AI. Open cows during the previous two phases were exposed to the bull. Results. Pregnancy in phase 1 was different (p<0.01) for PP and OC groups, 58.6% and 34.1%, respectively. Overall pregnancy percentage over the second service was 42.5% (p>0.05). No differences (p>0.05) were observed at phase 3, average 44.2%. By the end of the breeding season, the cost of OC, was 3 times more than PP cows. Conclusions. Pregnancy rate at first phase was higher in PP cows than OC cows. At the end of breeding season, a pregnancy rate of 80% was found. Incorporation of open cows from previous breeding season was more expensive than PP cows in all phases of the breeding program. Retaining an open cow for rebreeding one year or more could not be economically feasible.

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