scholarly journals HEAVY METAL-RESISTANT RHIZOBACTERIA FROM GOLD MINE IN PONGKOR INDONESIA AND COPPER MINE IN MARINDUQUE PHILIPPINES

Agric ◽  
2019 ◽  
Vol 31 (1) ◽  
pp. 75-88 ◽  
Author(s):  
Erny Yuniarti ◽  
Ida F Dalmacio ◽  
Erlinda S Paterno
Keyword(s):  
Resistance Mechanism ◽  
Morphological Characterization ◽  
Gold Mine ◽  
Isolation Medium ◽  
Mine Site ◽  
Copper Mine ◽  
Gram Staining ◽  
16S Rna ◽  
Agar Media

The purposes of the study was to isolate, to characterize, and to identify rhizobacteria from plant rhizosphere growing in gold and copper mine. The isolation of rhizobacteria used N-free semisolid agar media, TSA, and SLP plus heavy metals (HMs), namely Pb, Cd, and or Cu. Isolated rhizobacteria were subsequently characterized for resistance to higher level of Pb, Cd, Cu in SLP media. Cultural and morphological characterization of rhizobacteria were conducted for cell morphology, motility, Gram staining, and biofilm formation. The rhizobacteria identification used sequence analysis of the 16S RNA gene fragments. The results showed that the majority of rhizobacterial from Cu mine site (66.7% of 21 isolates) were resistant to Cu (72150 ppm) while the majority of rhizobacteria from gold mine site (77.8% of 18 isolates) were sensitive to 72 ppm Cu. Majority of Cu in the soil was insoluble as granules attaching to gravel so that rhizobacteria of Cu mine site have been exposed and adapted to available Cu. This fact, explaining that the rhizobacteria’s MIC value was lower than the total Cu level in the soil. Three HMs-resistant rhizobacter (PbSM 2.1, MGR 334, and CuNFbM 4.1) formed biofilms, which was as one of the resistance mechanism to HMs. This research informed that HM contaminated-soil is better source for obtaining HM resistant rhizobacteria than HM uncontaminated-soil. The use four isolation media produce rhizobacteria which was more diverse than rhizobacteria from each isolation medium. Further characterization needs to be done to obtain HM resistant-rhizobacteria which can be used as biofertilizers and phytoremediation agent.

scholarly journals EXTRACTION & PURIFICATION OF HEMOLYSIN FROM TOXIN PRODUCING MICROORGANISMS AND ITS EFFECT ON FORENSICALLY IMPORTANT BLOOD AND SALIVA SAMPLES

2019 ◽  
Vol 2 (4) ◽  
pp. 76
Author(s):  
MohammedAzim Bagban ◽  
Nilam Parmar ◽  
Nayan Jain
Keyword(s):  
Dna Analysis ◽  
Amylase Activity ◽  
Antimicrobial Property ◽  
Morphological Characterization ◽  
Gram Staining ◽  
Sds Page ◽  
Agar Media ◽  
Crucial Part ◽  
Blood Grouping

Examination of Blood and saliva samples in forensic investigation is now become crucial part of judiciary as it can be useful for the identification of an individual. Individual can be identified from such biological samples by blood grouping as well as DNA analysis. In the study isolation of microorganisms was done from different surfaces having highest possibility of presence of biological evidence, Gram staining and morphological characterization of microorganisms was done, the extraction of toxin from toxin producing microorganism which was determined by the zone of hemolysis on blood agar media was done by ammonium sulphate and sodium azide method. Purification was done and confirmation of toxin was done by ditch method, TLC & SDS-PAGE. Effect of toxin on blood was examined by Drabkin’s method for determining hemolytic unit. Effect of hemolysin on saliva was examined by starch hydrolysis test as well as loss of an antimicrobial Property of saliva.  As the toxin isolated is hemolysin toxin means it degrades the blood, but in the study we observed the effect of the hemolysin toxin on saliva as well. As mentioned in results blood samples loses its ABO blood grouping activity as it got affected by toxin. Saliva was also highly affected by toxin isolated, as the saliva loses the amylase activity and antimicrobial activity as well. The study is significant in cases where blood and saliva may found as crucial evidence. As RBCs and surface antigens got degraded by hemolysin blood as evidence may be undetectable by presumptive test and may give false results. Saliva samples also become untraceable during the presumptive test as loses its amylase activity.

scholarly journals Morphological characterization of gram-positive and gram-negative bacteria from treated latex processing wastewater

2021 ◽  
Vol 1 (2) ◽  
pp. 32-36
Author(s):  
Farrah Aini Dahalan ◽  
Nor Azizah Parmin
Keyword(s):  
Oxygen Demand ◽  
Morphological Characterization ◽  
The Other ◽  
Morphological Properties ◽  
Morphological Observation ◽  
Gram Positive ◽  
Bacterial Colony ◽  
Gram Negative ◽  
Gram Staining

A preliminary morphological screening and isolation of bacterial colony from latex industrial wastewater was carried out. Bacteria colonies from latex processing wastewater were isolated from a local latex processing industry. It was found that 17 bacterial isolates had been purified grown on nutrient agar under 35˚C. The colonies were then purified and morphologically indicated via Gram staining and motility test. After morphological observation, it was identified that out of 17 isolates, 9 isolates were Gram positive and 8 isolates were Gram negative. There are 11 out of 17 colonies were rod-shaped bacterial colonies, while the other 6 colonies were cocci-shaped bacteria. There were 11 colonies of gliding bacteria, three colonies were non-motile bacteria and the other three colonies were flagellated bacteria. This study is only limited to morphological observation as the main aim of this study was to investigate the potential occurrence of viable growth in treated latex processing wastewater. The bacterial colonies were classified base on their morphological properties shown. This study has classified several genera such as Staphylococcus, Escherichia, Thiobacillus, Arthrobacter and other Genus. The growth curve of 17 isolates studied and the chemical oxygen demand were determined.

scholarly journals CHARACTERIZATION OF BACTERIAL ISOLATES FOR SUSTAINABLE RICE BLAST CONTROL

2020 ◽  
Vol 33 (3) ◽  
pp. 702-712
Author(s):  
BÁRBARA ESTEVAM DE MELO MARTINS ◽  
AMANDA ABDALLAH CHAIBUB ◽  
MARCIO VINICIUS DE CARVALHO BARROS CORTÊS ◽  
VALÁCIA LEMES DA SILVA LOBO ◽  
MARTA CRISTINA CORSI DE FILIPPI
Keyword(s):  
Rice Blast ◽  
Pcr Amplification ◽  
Morphological Characterization ◽  
Bacterial Isolates ◽  
Specific Primers ◽  
Leaf Blast ◽  
Fluorescent Pigment ◽  
Gram Staining ◽  
Bacillus Spp

ABSTRACT Rice blast (Magnaporthe oryzae) limits rice (Oryza sativa) grain yields worldwide. The objective of this investigation was to morphologically, biochemically, and molecularly characterize six bacterial isolates, BRM 32109, BRM 32110, BRM 32111, BRM 32112, BRM 32113, and BRM 32114, and to determine their potential as antagonists to M. oryzae. Morphological characterization was based on colony formation and color, Gram staining, and fluorescent pigment production. Biochemical studies were based on cellulase, chitinase, phosphatase, indoleacetic acid, and siderophore production, as well as biofilm formation. The molecular identification used specific primers for PCR amplification of the 16S rRNA region, followed by sequencing. The antagonism studies involved three experiments, which had randomized designs. Two of them were conducted in laboratory conditions, pairing bacterial colonies and M. oryzae, using bacterial filtrates, and the third was conducted in greenhouse conditions. BRM 32111 and BRM 32112 were identified as Pseudomonas sp., BRM 32113 as Burkholderia sp., BRM 32114 as Serratia sp., and BRM 32110 and BRM 32109 as Bacillus spp. BRM 32112, BRM 32111, and BRM 32113 inhibited the colony of M. oryzae by 68%, 65%, and 48%, respectively. The bacterial suspensions of the BRM 32111, BRM 32112, and BRM 3212 filtrates suppressed leaf blast by 81.0, 79.2, and 66.3%, respectively. BRM 32111 and BRM 32112 were determined to be antagonists of M. oryzae and were found to solubilize phosphate, produce siderophores and cellulose, form biofilms, and suppress leaf blast. These isolates should be further investigated as potential biological control agents for leaf blast control.

CHARACTERIZATION OF THE WASTE ROCK AND PIT WALLS AT THE JUNDEE GOLD MINE SITE IN WESTERN AUSTRALIA AND IMPLICATIONS FOR LONG-TERM ISSUES

2006 ◽  
Vol 2006 (2) ◽  
pp. 1961-1973
Author(s):  
Shannon Shaw ◽  
Jason Martin ◽  
Greg Meiers ◽  
Mike O’Kane ◽  
Christoph Wels
Keyword(s):  
Western Australia ◽  
Waste Rock ◽  
Gold Mine ◽  
Mine Site

scholarly journals Karakterisasi Morfologi Phytophthora sp. Asal Buah Kakao Desa Olo-oloho, Kabupaten Konawe, Sulawesi Tenggara

2020 ◽  
Vol 7 (1) ◽  
pp. 1064
Author(s):  
Muzuni Muzuni ◽  
Haidin Haidin ◽  
Nur Arfa Yanti
Keyword(s):  
Cell Morphology ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Point Method ◽  
Colony Morphology ◽  
Vegetable Juice ◽  
Agar Media

This study aimed to determine the morphological characteristics of Phytophthora sp. isolated from cocoa fruits from Olo-oloho Village, Konawe Regency, Southeast Sulawesi. Isolation of Phytophthora sp. carried out by the point method using V4 (Vegetable Juice Agar) media incubated at 27ºC for 24 hours. Morphological characterization of Phytophthora sp. included characterization of colony morphology and cell morphology. The results showed that the colony morphological characteristics were white colonies, cotton-like textures, the uneven edge of the colony, zoning and radial lines. The morphological characteristics of the cell had asexual spores in the form of sporangium and chlamydospores, hyphae are not aseptic, greenish-black zoospores, zoospores are round and double-flagged, and have sporangiophores. Keywords: Phytophthora sp., colony morphology, cell morphology

scholarly journals Morphophysiological characterization of Ceratocystis fimbriata isolates from yerba mate

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
Nícolas Manarim de Brito ◽  
Henrique da Silva Silveira Duarte ◽  
Caroline de Bastos Bühner ◽  
Celso Garcia Auer ◽  
Álvaro Figueredo dos Santos
Keyword(s):  
Mycelial Growth ◽  
Culture Medium ◽  
Morphological Characterization ◽  
Yerba Mate ◽  
Optimum Temperature ◽  
Ceratocystis Fimbriata ◽  
Agar Media

ABSTRACT: This study aimed to morphologically characterize the isolates of Ceratocystis fimbriata from yerba mate and to evaluate the effect of culture medium and temperature on mycelial growth and sporulation of C. fimbriata. For the morphological characterization of the 11 monosporic isolates of the fungus, slides were prepared to determine the dimensions of the sexual and asexual structures of the fungus. Four experiments were conducted to evaluate the mycelial growth and to evaluate the sporulation of C. fimbriata in different culture mediums and temperatures. The isolates of C. fimbriata from yerba mate showed perithecia with brown to black necks, divergent ostiolar hyphae, hatshaped hyaline ascospores, single-celled, cylindrical endoconidia, and globular to ovoid aleurioconidia. PDA and V8-agar media showed the highest mycelial growth. The average optimum temperature for mycelial growth and sporulation of isolates of C. fimbriata of yerba mate were 22.5 and 22.4 ºC, respectively.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Morphological Characterization of Rhinacanthus Species of Sri Lanka

Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara
Keyword(s):  
Sri Lanka ◽  
Morphological Characterization
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