scholarly journals Yacon Flour and Corn Steep Liquor as Substrate for Inulinase and Biomass Production by Kluyveromyces Marxianus NRRL Y-7571

2014 ◽  
Vol 4 (3) ◽  
pp. 414-423
Author(s):  
Leonardo Amorin Sathler ◽  
Jonas Contiero ◽  
Marcia Luciana Cazetta
Keyword(s):  
Biomass Production ◽  
Kluyveromyces Marxianus ◽  
Enzyme Production ◽  
Corn Steep Liquor ◽  
Surface Response Methodology ◽  
Perennial Plant ◽  
High Fructose ◽  
Steep Liquor ◽  
Central Composite ◽  
Optimal Ph

Yacon is a perennial plant native of the Andesthat stores inulin-type polyfructans as reserve carbohydrates in its tubers. The enzymes that hydrolyze the inulin β-2,1 fructose  linkages are called inulinases and are much used to produce high fructose syrups (HFS) and ethanol. The present study investigated inulinase and biomass production by Kluyveromyces marxianus NRRL Y-7571 using yacon flour as carbon source and corn steep liquor as nitrogen source by a Central Composite Design (CCD) 22 to carry out experiments and Surface Response Methodology (RSM) to analyze the results. The maximum enzyme production was 36.1 U.ml-1 and biomass production was 8.95 g.l-1 under the condition of 7% yacon flour and 2% corn steep liquor. The crude enzyme partial characterization showed that inulinase had optimal pH at 4.0 and optimal temperature at60°C. The enzyme maintained over 90% of its activity for four hours at45°C and50°C.

scholarly journals Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates

2020 ◽  
Vol 21 (12) ◽  
pp. 4286
Author(s):  
Adriana Ferreira de Souza ◽  
Hugo Marques Galindo ◽  
Marcos Antônio Barbosa de Lima ◽  
Daylin Rubio Ribeaux ◽  
Dayana Montero Rodríguez ◽  
...  
Keyword(s):  
Corn Steep Liquor ◽  
Nitrogen Sources ◽  
Central Point ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Deacetylation Degree ◽  
Biomass Yields ◽  
Steep Liquor ◽  
Central Composite ◽  
Cassava Waste

We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266. The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus. The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL). The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains. The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively. M. subtilissimus (UCP 1262) showed dimorphism in assay 4–6% CSL and 8% CWW and central point. L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW. The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.

Optimization and kinetics studies on exopolysaccharide production using Klebsiella oxytoca ICCF 419 strain, and glucose as substrate

2021 ◽  
Vol 26 (1) ◽  
pp. 2214-2222
Author(s):  
CATERINA TOMULESCU ◽  
MIȘU MOSCOVICI ◽  
ROXANA MĂDĂLINA STOICA ◽  
GABRIEL BUJOR ALBU ◽  
CLAUDIA SEVCENCO ◽  
...  
Keyword(s):  
Corn Steep Liquor ◽  
Klebsiella Oxytoca ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Logistic Equations ◽  
Maximum Cell ◽  
Steep Liquor ◽  
Central Composite ◽  
Optimal Formula

The present work aimed to investigate the efficiency of kinetic modeling and Response Surface Methodology (RSM), by employing a Central Composite Rotatable Design (CCRD) and to evaluate the effects of carbon and nitrogen sources, inoculation volume, and bioprocess time on an exopolysaccharide (EPS) producer, Klebsiella oxytoca ICCF 419, in batch fermentation with shaken flasks. Among the kinetic models tested, logistic equations were found to fit accordingly, with a correlation coefficient (R2 ) of 0.999. The interaction between glucose and corn steep liquor was found to have a significant impact on the EPS production, and glucose (2% w/v), and a 5% v/v inoculum of 24 hours age, are sufficiently to positively affect the bioprocess, if it is desired only a maximum cell growth, but not associated with the polymer synthesis. The optimal formula predicted by the RSM design was experimentally validated by the following results: 12.16 g EPS/L and 1.65 dry biomass/L, after 48 hours fermentation.

scholarly journals Addition of molasses, corn steep liquor, and rice polish as economical sources to enhance the fungal biomass production of wheat straw by Arachniotus sp.

2017 ◽  
Vol 41 ◽  
pp. 332-336
Author(s):  
Faisal SHAHZAD ◽  
Muhammad ABDULLAH ◽  
Abdul Shakoor CHAUDHRY ◽  
Abu Saeed HASHMI ◽  
Jalees Ahmed BHATTI ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Biomass Production ◽  
Fungal Biomass ◽  
Corn Steep Liquor ◽  
Steep Liquor

scholarly journals Extra- and intra-cellular lipases from a thermophilic Rhizopus oryzae and factors affecting their production

1993 ◽  
Vol 39 (10) ◽  
pp. 978-981 ◽  
Author(s):  
A. B. Salleh ◽  
R. Musani ◽  
M. Basri ◽  
K. Ampon ◽  
W. M. Z. Yunus ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Rhizopus Oryzae ◽  
Corn Steep Liquor ◽  
Carbon Sources ◽  
Extracellular Enzyme ◽  
Lipase Production ◽  
Intracellular Enzyme ◽  
Factors Affecting ◽  
Positive Effects ◽  
Steep Liquor

A thermophilic Rhizopus oryzae was isolated, and parameters affecting its production of extra- and intra-cellular lipases were investigated. All carbon sources tested with the exception of sucrose generally inhibited the production of extracellular lipase, but enhanced the production of intracellular lipase. Peptone was the best substrate for extracellular enzyme production, but for intracellular lipase production other substrates such as tryptone, tryptic soy digest, polypeptone, and corn steep liquor gave comparable results. Among lipid substrates, glycerol was the only stimulator of extracellular enzyme production, whereas olive oil, triolein, and oleic acid had very positive effects on intracellular enzyme production. Shaking enhanced the production of both types of enzymes; the temperature optima were 45 and 37 °C for extra- and intra-cellular lipases, respectively. A pH of 5.0 was optimal for production of both enzymes.Key words: lipases, Rhizopus oryzae, production.

scholarly journals Byproducts from cassava industry: alternative substrates for cyclodextrin glycosyltransferase production by alkalophilic Bacillus trypoxylicola SM–02

2020 ◽  
pp. 37-46
Author(s):  
Carine Mascena Peixoto ◽  
Sheila Lorena de Araújo Coelho ◽  
Marcia Luciana Cazetta
Keyword(s):  
Corn Steep Liquor ◽  
Relative Activity ◽  
Optimum Temperature ◽  
Cyclodextrin Glycosyltransferase ◽  
Cassava Wastewater ◽  
Steep Liquor ◽  
Alkalophilic Bacillus ◽  
Central Composite ◽  
Cassava Peel

Bioproductos de la industria de la yuca: sustratos alternativos para la producción de ciclodextrina glucosiltransferasa por alcalófilo Bacillus trypoxylicola SM-02 En el presente trabajo estudiamos el uso licor de maíz fermentado (LMF), harina de cáscara de yuca (HCY) y aguas residuales de yuca para la producción de ciclodextina glicosiltransferasa (CGTase) por un nuevo aislado alcalófilo de Bacillus trypoxylicola SM-02 en fermentación sumergida. Los experimentos se realizaron por Diseño Central Compuesto Rotativo 22 totalizando 11 ensayos. La mayor actividad enzimática de 352.53 U/mL se obtuvo con 1.5 g de HCY y 0.6 g de LMF. La temperatura y el pH óptimos fueron 55 ºC y pH 8.0, respectivamente. CGTase mostró una actividad relativa superior al 50% durante 120 min. a la temperatura óptima. Solo el CaCl2 mostró actividad positiva para CGTasa. Los resultados apuntaron a un buen potencial de B. trypoxylicola SM-02 para la producción de CGTasa usando substratos residuales. In the present work was studied the use of cassava peel flour (CPF), corn steep liquor (CSL), and cassava wastewater as substrates to produce cyclodextrin glycosyltransferase (CGTase) from a new alkalophilic isolate of Bacillus trypoxylicola SM-02 by submerged fermentation. The experiments were performed as a Central Composite Design 22, totalizing 11 assays. An enzymatic activity of 352.53 U/mL was obtained using 1.5 g of CPF and 0.6 g of CSL. The optimum temperature and pH of CGTase was 55 °C and 8.0, respectively. The CGTase depicted a relative activity of more than 50% for 120 min at the optimum temperature. The only salt that positively influenced the CGTase activity was CaCl2. The results are indicative of a potential role of B. trypoxylicola SM-02 in the production of CGTase using residual substrates.

scholarly journals Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571

2016 ◽  
Vol 38 (3) ◽  
pp. 283
Author(s):  
Luana Paula de Azevedo de Oliveira ◽  
Tiago Felipe Oliveira ◽  
Jonas Contiero ◽  
Márcia Luciana Cazetta
Keyword(s):  
Thermal Stability ◽  
Central Composite Design ◽  
Yeast Extract ◽  
Kluyveromyces Marxianus ◽  
Crude Enzyme ◽  
Partial Characterization ◽  
Central Composite ◽  
Optimal Ph

The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. 

Low concentration of corn steep liquor promotes seed germination, plant growth, biomass production and flowering in soybean

2018 ◽  
Vol 87 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Mi-Mi Zhu ◽  
En-Qi Liu ◽  
Yan Bao ◽  
Shi-Long Duan ◽  
Jiao She ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Biomass Production ◽  
Corn Steep Liquor ◽  
Low Concentration ◽  
Steep Liquor

scholarly journals Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Abbas Akhavan Sepahy ◽  
Leila Jabalameli
Keyword(s):  
Enzyme Production ◽  
Corn Steep Liquor ◽  
Experimental Studies ◽  
Environmental Parameters ◽  
Nitrogen Sources ◽  
Culture Conditions ◽  
Maximum Activity ◽  
Protease Production ◽  
North East ◽  
Steep Liquor

Soil samples of Tehran jungle parks were screened for proteolytic Bacilli. Among eighteen protease producers one of the isolates obtained from Lavizan park, in north east of Tehran, was selected for further experimental studies. This isolate was identified as Bacillus sp. strain CR-179 based on partial sequencing of 16S rRNA. Various nutritional and environmental parameters affected protease production by Bacillus sp. strain CR-179. Protease production by this Bacillus cultivated in liquid cultures reached a maximum at 24 h, with levels of 340.908 U/mL. Starch and maltose were the best substrates for enzyme production while some pure sugars such as fructose, glucose, and sucrose could not influence production of protease. Among various organic nitrogen sources corn steep liquor, which is commercial, was found as the best substrate followed by yeast extract, whey protein, and beef extract. The optimal pH and optimal temperature of enzyme production were 8.0 and 45°C, respectively. Studies on enzymatic characterization revealed that crude protease showed maximum activity at pH 9.0 and 60°C, which is indicating the enzyme to be thermoalkaline protease.

Application of response surface methodology and central composite rotatable design (CCDR) for modelling the influence of agro-industrial waste in lactic acid biosynthesis

2019 ◽  
Vol 6 (4) ◽  
pp. 51
Author(s):  
Ana Paula Testa Pezzin ◽  
Jaqueline Boldt Capellari ◽  
Eduarda Zeni Neves ◽  
Michele Cristina Formolo Garcia ◽  
Giannini Pasiznick Apati ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Experimental Design ◽  
Industrial Waste ◽  
Corn Steep Liquor ◽  
Microbial Culture ◽  
Central Composite Rotatable Design ◽  
Wide Range ◽  
Steep Liquor ◽  
Central Composite ◽  
Rotatable Design

Lactic acid (LA) is one of the most important organic acids, with a wide range of industrial and biotechnological applications and can be produced by chemical synthesis and microbial culture. However, the biotech pathway is generally preferred because it provides an optically pure product. In this context, the purpose of this work was to evaluate LA biosynthesis by Lactobacillus amylovorus using molasses as carbon source (CS) and corn steep liquor as nitrogen source (NS) in a central composite rotatable design (CCRD) varying the concentration CS and NS, as well as to validate the model. The method for microbial culture followed an experimental design of the CCRD type, conducted without agitation, at 37ºC in Erlenmeyer flask, with pH in spontaneous evolution. The results showed that, using molasses and corn steep liquor as alternative sources, LA production ranged from 2.8 to 4.6 g/L, respectively, with the most favourable condition being 40.0 g of molasses and 250 g of corn steep liquor. It was possible, from the experimental design, to ascertain the selection of the best conditions for the microbial culture, demonstrating the feasibility of replacing CS and NS by agro-industrial waste, thus reducing the cost of producing LA.

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