scholarly journals SOIL-BORNE MICROORGANISMS THREATENING CARROT CULTIVATED WITH THE USE OF COVER CROPS

2020 ◽  
Vol 19 (4) ◽  
pp. 71-86 ◽  
Author(s):  
Elżbieta Patkowska
Keyword(s):  
Cover Crops ◽  
Soil Microorganisms ◽  
Pathogenic Fungi ◽  
Trichoderma Spp ◽  
Saprotrophic Fungi ◽  
Daucus Carota L ◽  
Alternaria Dauci ◽  
Bacillus Spp ◽  
Positive Effect ◽  
Soil Borne Fungi

Cover crops are used in the cultivation of various plants. They properly modify the composition of soil microorganisms and can protect of plants from phytopathogens. The purpose of the field and laboratory studies was to determine the quantitative and qualitative composition of microorganisms in the soil under carrot cultivated with the use of oats, tansy phacelia and spring vetch as cover crops. The paper presents also studies on soil-borne fungi threatening the healthiness of carrot roots. In the conventional cultivation of carrot the population of bacteria (including Pseudomonas spp. and Bacillus spp.) was the smallest, while after the application of oats it was the largest. Oats and spring vetch were most effective in limiting the occurrence of soil-borne fungi. Those plants and tansy phacelia caused an increase of the population of saprotrophic fungi (Albifimbria spp., Clonostachys spp. and Trichoderma spp.) in the soil. Intercrop plants had a positive effect on the healthiness of carrot seedlings and roots. Alternaria dauci, A. alternata, A. radicina, Fusarium oxysporum, Globisporangium irregulare, Neocosmospora solani, Phytophthora sp., Rhizoctonia solani and Sclerotinia sclerotiorum proved to be the most harmful towards the studied underground parts of carrot. Oats proved to be the most effective in inhibiting the occurrence of the pathogenic fungi for Daucus carota L.

scholarly journals Effect of root exudates of various plants on composition of bacteria and fungi communities with special regard to pathogenic soil-borne fungi

2013 ◽  
Vol 54 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Danuta Piętka ◽  
Elżbieta Patkowska
Keyword(s):  
Winter Wheat ◽  
Root Exudates ◽  
Rhizosphere Soil ◽  
Pathogenic Fungi ◽  
Microbiological Analysis ◽  
Soybean Roots ◽  
Bacteria And Fungi ◽  
Positive Effect ◽  
Number Of Bacteria ◽  
Soil Borne Fungi

The purpose of the studies conducted in the years 1996 - 1998 was to determine the composition of bacteria and fungi populations in the rhizosphere of winter wheat, spring wheat, soybean and potato, and in non-rhizosphere soil. Besides, the effect of root exudates of these plants on the formation of pathogenic fungi communities was established. The microbiological analysis showed that the greatest tolal number of bacteria was found in the rhizospheres of potato and soybean, and the lowest number in non-rhizosphere soil. The smallest total number of fungi was found in the rhizosphere of winter wheat, and the largest in the rhizosphere of soybean. Pathogenic fungi dominated in the rhizospheres of soybean and potato, while non-rhizosphere soil was the poorest in these microorganisms. Among the pathogenic fungi, <i>Fusarium oxysporum, F.culmorum</i> and <i>F.solani</i> were most frequently isolated. Soybean roots exudated the greatest amount of aminoacids, and acidic aminoacids, which have a positive effect on the development of phytopathogens, dominated in their content. On the other hand, the best quantitative and qualitative composition of aminoacids was found out in the root exudates of winter wheat, since they conlained big amounts of alkaline and aromatic aminoacids.

scholarly journals COVER CROPS AND SOIL-BORNE FUNGI DANGEROUS TOWARDS THE CULTIVATION OF Daucus carota L.

2021 ◽  
Vol 20 (2) ◽  
pp. 3-12
Author(s):  
Elżbieta Patkowska
Keyword(s):  
Cover Crops ◽  
Daucus Carota ◽  
Experimental Treatment ◽  
Soil Tillage ◽  
Quantitative Composition ◽  
White Mustard ◽  
Cultivation System ◽  
Daucus Carota L ◽  
Horticultural Practice ◽  
Soil Borne Fungi

The principles of good agricultural and horticultural practice, which considers both environmental protection and high yielding of plants, require modern methods of cultivation. Cover crops are used in the cultivation of various plants, including root vegetables such as carrot. The purpose of field and laboratory studies was to determine the effect of selected cover crops on the healthiness of carrot (Daucus carota L.). The field experiment took into consideration cover crops such as spring rye, white mustard, buckwheat, fodder sunflower and two systems of soil tillage, i.e.: tillage before winter (ploughing) + spring tillage (a combined cultivator) and only spring tillage (a combined cultivator). In each experimental treatment, the number and healthiness of carrot seedlings were determined. A laboratory mycological analysis made it possible to determine the qualitative and quantitative composition of fungi infecting the underground parts of carrot. Good emergencies and the healthiness of carrot plants were observed in the objects with rye and white mustard as cover crops. The cultivation system had no significant effect on the proportion of diseased seedlings of carrot. Rye and white mustard were more effective than buckwheat and sunflower in limiting  the occurrence of fungi pathogenic towards carrot. Diseased seedlings and roots after harvest of carrot were most frequently colonized by Altenaria alternata, A. chartarum, A. dauci, A. radicina, Sclerotinia sclerotiorum, Rhizoctonia solani and Fusarium spp.

scholarly journals Antagonistic activity of selected bacteria occurring in the soil after root chicory cultivation

2014 ◽  
Vol 60 (No. 7) ◽  
pp. 320-324 ◽  
Author(s):  
E. Patkowska ◽  
M. Konopiński
Keyword(s):  
Cover Crops ◽  
Total Population ◽  
Fusarium Culmorum ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Common Vetch ◽  
Mineral Salts ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Positive Effect

Chicory (Cichorium intybus L.) is a rich source of polysaccharides (intibin and inulin), vitamins, acids and mineral salts. The present studies determined the antagonistic activity of selected bacteria of the soil environment of root chicory towards fungi pathogenic towards this plant: Alternaria alternata, Botrytis cinerea, Fusarium culmorum, F. oxysporum, Thanatephorus cucumeris and Sclerotinia sclerotiorum. Root chicory was cultivated with using cover crops (oats, tansy phacelia and common vetch). The use of cover crops, especially oats, in the cultivation of root chicory had a positive effect both on the total population of bacteria and the population of Bacillus spp. and Pseudomonas spp. in the soil. Antagonistic Bacillus spp. and Pseudomonas spp. displayed differentiated activity towards the studied fungi. The greatest antagonistic effect was found out after the mulch of oats. After common vetch, the antagonistic activity of bacteria was more than twice, and after phacelia three times as low as after oats. In the traditional cultivation of root chicory this activity was nearly twelve times lower.

scholarly journals Antagonistic bacteria in the soil after cover crops cultivation

2014 ◽  
Vol 60 (No. 2) ◽  
pp. 69-73 ◽  
Author(s):  
E. Patkowska ◽  
M. Konopiński
Keyword(s):  
Cover Crops ◽  
Total Population ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Quantitative Composition ◽  
Antagonistic Bacteria ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Soil Borne Fungi

The purpose of the studies was to establish the quantitative composition of bacteria Bacillus spp. and Pseudomonas spp. and their antagonistic effect towards soil-borne fungi after the cultivation of oats, spring vetch and tansy phacelia as intercrop cover plants. The total population of bacteria in 1 g dry weight of the soil from the experimental combination where mulch of oats was used was larger than in the combination with spring vetch or tansy phacelia. Totally, approximately three times as much Bacillus spp. was obtained from soil samples as compared to Pseudomonas spp. Tests showed that the most isolates of antagonistic bacteria out of the enumerated genera occurred in the soil after oats cultivation, and the least in the soil after the cultivation of tansy phacelia. Antagonistic Bacillus spp. and Pseudomonas spp. inhibited the growth and development of Fusarium oxysporum, Haematonectria haematococca and Thanatephorus cucumeris in the most effective way. The greatest total antagonistic effect of Bacillus spp. and Pseudomonas spp. towards Altenaria alternata, F. culmorum, F. oxysporum, H. haematococca, P. irregulare and&nbsp;T. cucumeris was found out after managing the mulch of oats. The smallest total antagonistic effect of bacteria was observed after managing the mulch of tansy phacelia.

scholarly journals Antagonistic fungi in the soil after Daucus carota L. cultivation

2019 ◽  
Vol 65 (No. 3) ◽  
pp. 159-164 ◽  
Author(s):  
Elżbieta Patkowska ◽  
Agnieszka Jamiołkowska ◽  
Elżbieta Mielniczuk
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Daucus Carota ◽  
Antagonistic Activity ◽  
Laboratory Studies ◽  
White Mustard ◽  
Trichoderma Spp ◽  
Daucus Carota L ◽  
Antagonistic Fungi ◽  
Penicillium Spp ◽  
Soil Borne Fungi

Field and laboratory studies determined the effect of intercrop plants (rye, buckwheat, white mustard and sunflower) used in carrot cultivation on the occurrence of Clonostachys spp., Albifimbria spp., Trichoderma spp. and Penicillium spp. in the soil and their antagonistic activity. Rye and white mustard were the most effective in limiting the occurrence of soil-borne fungi. Those plants caused an increase of the population of the studied antagonistic fungi. Buckwheat and sunflower promoted the development of antagonists a little worse. The antagonistic activity of the aforementioned fungi was the highest after using rye and white mustard and slightly after buckwheat and sunflower. Those fungi were the most effective in inhibiting the growth of Altenaria dauci, A. radicina and Sclerotinia sclerotiorum pathogenic towards carrot.

scholarly journals Antagonistic bacteria in the soil after Daucus carota L. cultivation

2018 ◽  
Vol 64 (No. 3) ◽  
pp. 120-125
Author(s):  
Patkowska Elżbieta
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Daucus Carota ◽  
Pathogenic Fungi ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Antagonistic Bacteria ◽  
White Mustard ◽  
Daucus Carota L ◽  
Pseudomonas Spp ◽  
Bacillus Spp

The studies determined the effect of bacteria Bacillus spp. and Pseudomonas spp. isolated from the soil after carrot cultivation on pathogenic fungi Altenaria dauci, A. radicina, Fusarium oxysporum, F. solani, Rhizoctonia solani and Sclerotinia sclerotiorum. A field experiment on carrot cultivation considered different intercrop plants (rye, buckwheat, white mustard, sunflower). Rye and buckwheat were the most conducive to the growth of Bacillus spp. and Pseudomonas spp. Those bacteria were the most effective in inhibiting the growth of F. solani, F. oxysporum and R. solani. The antagonistic effect of soil-borne Bacillus spp. and Pseudomonas spp. towards the tested fungi was the largest after the application of rye and white mustard as intercrop plants in the cultivation of carrot. Buckwheat and sunflower showed a slightly smaller influence on the antagonistic activity of the studied bacteria. Bacillus ssp. had a significantly lesser antagonistic effect than Pseudomonas ssp.

scholarly journals Toprak Kökenli Bitki Patojeni Funguslarla Mücadelede Biofumigasyonun Kullanım Olanakları

2019 ◽  
Vol 7 (9) ◽  
pp. 1245
Author(s):  
Sevilay Saygı ◽  
Muharrem Türkkan ◽  
İsmail Erper
Keyword(s):  
Cover Crops ◽  
Root Rot ◽  
Pathogenic Fungi ◽  
Soil Fumigation ◽  
Organic Fertilizers ◽  
Alternative Methods ◽  
Negative Effects ◽  
Horticultural Crops ◽  
Control Root ◽  
Soil Borne Fungi

The soil-borne plant pathogenic fungi, including Armillaria, Fusarium, Phytophthora, Rhizoctonia, Rosellinia and Sclerotinia etc, are causing root rot diseases on many field and horticultural crops that often results in the death of plants and important yield losses. Several control methods have been implemented to control root rot diseases, such as use of resistant cultivars, crop rotation, solarisation, fungicides treatments and soil fumigation. Soil fumigation with fumigants such as methyl bromide and chloropicrin is the most effective method for controlling soil-borne fungi; however, the negative effects of these chemicals on both the environmental and public health have led to its banning in many countries. Therefore, there is a need to find alternative methods which provide adequate disease control and is comparable to commercial fungicides. Bio fumigation is considered as an alternative to chemical control and is highly effective in the controlling of diseases caused by nematodes, weeds and fungi, and is considered as a part of integrated pest management. Cover crops, organic fertilizers and biosolids are considered as biofumigants in controlling of soil-borne fungi. In this study, bio fumigation applications in controlling of soil-borne fungi and their mode of action on the fungi are evaluated.

scholarly journals Cysteine-Rich Hydrophobin Gene Family: Genome Wide Analysis, Phylogeny and Transcript Profiling in Cordyceps militaris

2021 ◽  
Vol 22 (2) ◽  
pp. 643
Author(s):  
Xiao Li ◽  
Fen Wang ◽  
Yanyan Xu ◽  
Guijun Liu ◽  
Caihong Dong
Keyword(s):  
Life Cycle ◽  
Pathogenic Fungi ◽  
Class Ii ◽  
Cordyceps Militaris ◽  
Transcript Profiling ◽  
Saprotrophic Fungi ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Encoding Genes

Hydrophobins are a family of small secreted proteins found exclusively in fungi, and they play various roles in the life cycle. In the present study, genome wide analysis and transcript profiling of the hydrophobin family in Cordyceps militaris, a well-known edible and medicinal mushroom, were studied. The distribution of hydrophobins in ascomycetes with different lifestyles showed that pathogenic fungi had significantly more hydrophobins than saprotrophic fungi, and class II members accounted for the majority. Phylogenetic analysis of hydrophobin proteins from the species of Cordyceps s.l. indicated that there was more variability among the class II members than class I. Only a few hydrophobin-encoding genes evolved by duplication in Cordyceps s.l., which was inconsistent with the important role of gene duplication in basidiomycetes. Different transcript patterns of four hydrophobin-encoding genes during the life cycle indicated the possible different functions for each. The transcripts of Cmhyd2, 3 and 4 can respond to light and were related with the photoreceptors. CmQHYD, with four hydrophobin II domains, was first found in C. militaris, and multi-domain hydrophobins were only distributed in the species of Cordycipitaceae and Clavicipitaceae. These results could be helpful for further function research of hydrophobins and could provide valuable information for the evolution of hydrophobins.

scholarly journals Combining crop diversification practices can benefit cereal production in temperate climates

2021 ◽  
Vol 41 (4) ◽  
Author(s):  
Carolina Rodriguez ◽  
Linda-Maria Dimitrova Mårtensson ◽  
Erik Steen Jensen ◽  
Georg Carlsson
Keyword(s):  
Cover Crops ◽  
Cropping Systems ◽  
Grain Legume ◽  
Crop Diversification ◽  
Forage Legume ◽  
Cereal Crop ◽  
Spatial And Temporal Scales ◽  
N Acquisition ◽  
Positive Effect ◽  
Crop Sequence

AbstractDiversifying cropping systems by increasing the number of cash and cover crops in crop rotation plays an important role in improving resource use efficiency and in promoting synergy between ecosystem processes. The objective of this study was to understand how the combination of crop diversification practices influences the performance of arable crop sequences in terms of crop grain yield, crop and weed biomass, and nitrogen acquisition in a temperate climate. Two field experiments were carried out. The first was a 3-year crop sequence with cereal or grain legume as the first crops, with and without undersown forage legumes and forage legume-grass crops, followed by a cereal crop. The second experiment was a 2-year crop sequence with cereal or legume as the first crops, a legume cover crop, and a subsequent cereal crop. For the first time, crop diversification practices were combined to identify plant-plant interactions in spatial and temporal scales. The results partly confirm the positive effect of diversifying cereal-based cropping systems by including grain legumes and cover crops in the crop sequence. Legume cover crops had a positive effect on subsequent cereal grain yield in one of the experiments. Using faba beans as the first crop in the crop sequence had both a positive and no effect on crop biomass and N acquisition of the subsequent cereal. In cover crops composed of a forage legume-grass mixture, the grass biomass and N acquisition were consistently increased after the grain legume, compared to the cereal-preceding crop. However, differences in the proportion of legume to grass in mixture did not influence crop yield or N acquisition in the subsequent cereal. In conclusion, these results support that increased crop diversity across spatial and temporal scales can contribute to resource-efficient production and enhance the delivery of services, contributing to more sustainable cropping systems.

scholarly journals Cooperation among phosphate-solubilizing bacteria, humic acids and arbuscular mycorrhizal fungi induces soil microbiome shifts and enhances plant nutrient uptake

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Vincenza Cozzolino ◽  
Hiarhi Monda ◽  
Davide Savy ◽  
Vincenzo Di Meo ◽  
Giovanni Vinci ◽  
...  
Keyword(s):  
Microbial Community ◽  
Arbuscular Mycorrhizal Fungi ◽  
Humic Acids ◽  
Mycorrhizal Fungi ◽  
Soil Microorganisms ◽  
Microbial Community Composition ◽  
Arbuscular Mycorrhizal ◽  
Soil Microbiome ◽  
Phosphate Solubilizing Bacteria ◽  
Saprotrophic Fungi

Abstract Background Increasing the presence of beneficial soil microorganisms is a promising sustainable alternative to support conventional and organic fertilization and may help to improve crop health and productivity. If the application of single bioeffectors has shown satisfactory results, further improvements may arise by combining multiple beneficial soil microorganisms with natural bioactive molecules. Methods In the present work, we investigated in a pot experiment under greenhouse conditions whether inoculation of two phosphate-solubilizing bacteria, Pseudomonas spp. (B2) and Bacillus amyloliquefaciens (B3), alone or in combination with a humic acids (HA) extracted from green compost and/or a commercial inoculum (M) of arbuscular mycorrhizal fungi (AMF), may affect maize growth and soil microbial community. Phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis were performed to detect changes in the microbial community composition. Results Plant growth, N and P uptake, and mycorrhizal root colonization were found to be larger in all inoculated treatments than in the uninoculated control. The greatest P uptake was found when B. amyloliquefaciens was applied in combination with both HA and arbuscular mycorrhizal fungi (B3HAM), and when Pseudomonas was combined with HA (B2HA). The PLFA-based community profile revealed that inoculation changed the microbial community composition. Gram+/Gram− bacteria, AMF/saprotrophic fungi and bacteria/fungi ratios increased in all inoculated treatments. The greatest values for the AMF PLFA marker (C16:1ω5) and AMF/saprotrophic fungi ratio were found for the B3HAM treatment. Permutation test based on DGGE data confirmed a similar trend, with most significant variations in both bacterial and fungal community structures induced by inoculation of B2 or B3 in combination with HA and M, especially in B3HAM. Conclusions The two community-based datasets indicated changes in the soil microbiome of maize induced by inoculation of B2 or B3 alone or when combined with humic acids and mycorrhizal inoculum, leading to positive effects on plant growth and improved nutrient uptake. Our study implies that appropriate and innovative agricultural management, enhancing the potential contribution of beneficial soil microorganisms as AMF, may result in an improved nutrient use efficiency in plants.

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