scholarly journals First Report of Bean common mosaic necrotic virus Infecting Bean Plants in Aguascalientes and Veracruz, Mexico

Plant Disease ◽  
2000 ◽  
Vol 84 (8) ◽  
pp. 923-923 ◽  
Author(s):  
N. Flores-Estévez ◽  
L. Silva-Rosales ◽  
J. A. Acosta-Gallegos
Keyword(s):  
Bean Common Mosaic Virus ◽  
The State ◽  
Accession Number ◽  
Phaseolus Vulgaris L ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Bean Plants ◽  
Polymerase Chain ◽  
Alignment Analysis ◽  
Viral Isolates

Bean common mosaic necrosis virus (BCMNV) is a pathogen of Phaseolus vulgaris L. BCMNV was previously classified in serogroup A (for necrotic strains) of the Bean common mosaic virus (BCMV) subgroup; serogroup B included non-necrotic strains of BCMV. Both serogroups are currently classified as two different species in the BCMV subgroup of the Poytvirus genus; strains of either species will produce mosaic symptoms and, in the presence of hypersensitive I gene, necrosis in bean plants (1). Prior to this classification, BCMV was reported in Mexico by the presence of mosaic and necrotic symptoms (2). To investigate the presence of BCMNV in Mexico, samples were collected in two of the main bean producing states. The total extracted RNA was used for reverse transcription polymerase chain reaction using primers complementary to the middle portion of the NIa gene (5') and the 3' UTR of the NL3 Michigan isolate (accession number U19287). Two cDNA segments of 2 and 3 kb were obtained from infected plants from the state of Aguascalientes, in the highlands of central Mexico, and from the state of Veracruz, in the lowlands of the Gulf of Mexico, respectively. The cDNAs were cloned and sequenced. Alignment analysis of these sequences with the NL3 strain of BCMNV showed a similarity of 96.4 and 96.7%, respectively. The similarity between the Aguascalientes (accession number AJ01265) and Veracruz isolates was 99.6%, indicating that both are variants of the same species. On the other hand, alignment analysis of these isolates with some published BCMV strain sequences (i.e., accession numbers L15332 and U55315) displayed low similarities of 52.9 and 64.4%, respectively. These comparisons indicate that the Aguascalientes and Veracruz viral isolates belong to the BCMNV species and is the causal agent of mosaic and necrosis observed on the bean plants in those states. References: (1) C. W. Collmer et al. Mol. Plant-Microbe Interact. 9:758, 1996. (2) E. Jimenez-García. SARH. Mexico. D. F. pp: 3–4, 1994.

NL-3 K Strain Is a Stable and Naturally Occurring Interspecific Recombinant Derived from Bean common mosaic necrosis virus and Bean common mosaic virus

2005 ◽  
Vol 95 (9) ◽  
pp. 1037-1042 ◽  
Author(s):  
Richard C. Larsen ◽  
Phillip N. Miklas ◽  
Keri L. Druffel ◽  
Stephen D. Wyatt
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Molecular Size ◽  
Bean Common Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Bean Plants ◽  
Recombination Point ◽  
Russian Strain ◽  
Polymerase Chain

A strain of Bean common mosaic necrosis virus (BCMNV) from Idaho was identified by enzyme-linked immunosorbent assay using monoclonal antibodies and determined to be similar to the NL-3 D strain (of Drifjhout) by reaction of differential bean cultivars. However, this BCMNV strain (designated NL-3 K) caused earlier and more severe symptoms on bean plants representing host groups 0, 4, and 5. The nucleotide sequence encoding the predicted polyprotein of NL-3 K was 9,893 nucleotides (nt) in length, yielding a peptide with a molecular size of 362.1 kDa compared with a 9,626-nt, 350.9-kDa polyprotein for NL-3 D. Sequence analysis of the putative P1 protein suggests that the NL-3 K strain is a recombinant between NL-3 D and the Russian strain (RU1) of Bean common mosaic virus. The P1 protein of NL-3 K consisted of 415 amino acids compared with 317 for NL-3 D. The first 114 predicted amino acids of the NL-3 K P1 region were 98% identical with RU1. The remaining 301 amino acids of the protein shared only 34% identity with RU1 but were 98% identical with NL-3 D. Primers were designed that flanked the recombination point in the P1 coding sequence of NL-3 K. An amplicon of the expected size was produced by reverse-transcriptase polymerase chain reaction of total nucleic acid extracts of bean plants inoculated with NL-3 K, but not from those with NL-3 D or RU1. The increased symptom severity on selected common bean lines induced by NL-3 K suggests that the P1 gene may play a significant role in pathogenicity and virulence.

scholarly journals Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
J. Albertyn ◽  
K.M. Tajbhai ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Disease Virus ◽  
Common Disease ◽  
Sample Collection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus ◽  
Viral Isolates

Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.

scholarly journals First Molecular Identification of Sunfish in North Bali Water

2019 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
I Made Oka Riawan ◽  
Gede Iwan Setiabudi ◽  
I Made Merdana ◽  
I Putu Mangku Mariasa ◽  
Kadek Teguh Wirasastra
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Identification ◽  
Polymerase Chain Reaction Method ◽  
Accession Number ◽  
Chain Reaction ◽  
Reaction Method ◽  
D Loop ◽  
Reverse Primer ◽  
Polymerase Chain ◽  
Full Body

Stranded Sunfish in North Bali with full body we collect to do molecular identification. Samples were amplified at the d-loop locus (control region) using the PCR (Polymerase Chain Reaction) method. Primers used in PCR are H16498 as primary front (forward) and L15812 as reverse primer. Similarity value of 95% after alignment with Mola ramsayi (accession number accession AY940824) on GenBank, and the gaps of the nucleotide just 1%. The stranded sunfish identified using partial sequence mtDNA is the same species as the species Mola ramsayi.

scholarly journals First Record of Infectious Haematopoietic Necrosis Virus in Rainbow Trout Fry in Croatia

2007 ◽  
Vol 76 (1) ◽  
pp. 65-70 ◽  
Author(s):  
I. Vardić ◽  
D. Kapetanović ◽  
Z. Teskeredžić ◽  
E. Teskeredžić
Keyword(s):  
Rainbow Trout ◽  
Virus Detection ◽  
First Record ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Glycoprotein Gene ◽  
Phylogenetic Comparison ◽  
Infected Farm ◽  
Polymerase Chain

The paper describes the first diagnosis of infectious haematopoietic necrosis virus (IHNV) in Croatia. The viral causative agent was detected in pooled organ samples from the imported rainbow trout fry on the fish farm. Reverse transcriptase - semi-nested polymerase chain reaction (RT- snPCR) was applied directly on the infected tissue for rapid virus detection. After isolation on cell cultures, IHNV isolate was characterised on the basis of the 303 nt region of the glycoprotein gene (Mid-G) sequence. Phylogenetic comparison to North American and European IHNV isolates revealed that this Croatian isolate belongs to the M genogroup, confirming the prediction of the M genogroup origin of European IHNV isolates. The introduction of the virus presents a threat of further spreading of the disease in Croatia, as the infected farm is in a direct contact with the open waters.

scholarly journals Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

2016 ◽  
Vol 25 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Guilherme Maerschner Ogawa ◽  
Antonio Marques Pereira Júnior ◽  
Fábio Resadore ◽  
Ricardo de Godoi Mattos Ferreira ◽  
Jansen Fernandes Medeiros ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Breeding Site ◽  
Abundant Species ◽  
The State ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
First Time

Abstract This study had the aim of ascertaining the sandfly fauna and possible presence ofLeishmania in these insects, collected in caves in the state of Rondônia, Brazil. Collections were conducted in eight caves located in two different areas of this state. Leishmania in the sandflies collected was detected using the polymerase chain reaction (PCR). This was the first study on sandflies from caves in Rondônia and, among the total of 1,236 individuals collected, 24 species and 10 genera were identified. The speciesEvandromyia georgii was collected for the first time in Rondônia and the most abundant species were Trichophoromyia ubiquitalis with 448 individuals (36.2%), followed by T. octavioi with 283 (22.9%) and E. georgii with 179 (14.5%). For the PCR, 17 pools were analyzed and five pools were positive (forT. auraensis in three pools and for Nyssomyia shawi and N. antunesi in one pool each). The kDNA region was amplified and the presence of Leishmania DNA was confirmed. The sandfly fauna in these caves can be considered diverse in comparison with similar studies in other regions. It may be that some species use caves as a temporary shelter and breeding site, while other species live exclusively in this environment. The detection of LeishmaniaDNA indicates that this pathogen is circulating in cave environments and that further studies are needed in order to ascertain the risks of infection by leishmaniasis in these locations with high touristic potential.

scholarly journals Orbital Infection by Saksenaea vasiformis in an Immunocompetent Host

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Mingkwan Lumyongsatien ◽  
Pimkwan Jaru-ampornpan ◽  
Mongkol Uiprasertkul ◽  
Dinesh Selva
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Steroid Treatment ◽  
Histopathological Study ◽  
Immunocompetent Host ◽  
Accession Number ◽  
Chain Reaction ◽  
Upper Eyelid ◽  
Fungal Hyphae ◽  
Polymerase Chain

Orbital mucormycosis caused by Saksenaea vasiformis is extremely rare. Herein, we report an immunocompetent 22-year-old Thai female who presented with two months of progressive right upper eyelid mass, associated with swelling, redness, and ptosis. She failed to improve despite multiple courses of antibiotic and steroid treatment. Computed tomography (CT) scan showed infiltration involving the upper eyelid and lacrimal gland. Fungal hyphae were revealed by histopathological study. Polymerase chain reaction (PCR) was positive for Saksenaea vasiformis (GenBank: accession number FR687327.1). The patient was successfully treated with surgical debridement, amphotericin B, and oral posaconazole.

Sign in / Sign up

Export Citation Format

Share Document