Comparison of biochemical variables in plasma samples obtained from healthy dogs and cats by use of standard and microsample blood collection tubes

2010 ◽  
Vol 237 (3) ◽  
pp. 288-292
Author(s):  
Jacqueline C. Whittemore ◽  
Bente Flatland
Keyword(s):  
Creatine Kinase ◽  
Regression Analysis ◽  
Total Bilirubin ◽  
Pearson Correlation ◽  
Blood Collection ◽  
Plasma Samples ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Healthy Dogs ◽  
Blood Collection Tubes

Abstract Objective—To compare results of biochemical analyses performed on plasma samples obtained from healthy dogs and cats by use of standard and microsample blood collection tubes. Design—Evaluation study. Animals—29 healthy client-owned animals (14 dogs and 15 cats). Procedures—A blood sample (3 mL) was collected from each animal; 2.5 mL was transferred into a vacuum tube that contained lithium heparin, and 0.5 mL was transferred into a microsample tube that contained lithium heparin. Variables evaluated were albumin, bicarbonate, BUN, calcium, chloride, cholesterol, creatinine, glucose, phosphorus, potassium, sodium, total bilirubin, and total protein concentrations and alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, and creatine kinase activities. Results for the 2 types of tubes in each species were compared by use of Pearson correlation coefficients, Passing-Bablok regression analysis, and Bland-Altman analysis. Results—Data were normally distributed, except for creatine kinase activity of cats. The Pearson correlation coefficient was minimal for total bilirubin concentration in cats and moderate, high, or very high for all other variables. Constant bias for cholesterol and glucose concentration in dogs was identified during Bland-Altman analysis, although the mean difference between types of blood collection tubes was small. No constant or proportional bias for any other variable was revealed by regression analysis or Bland-Altman analysis. Conclusions and Clinical Relevance—Samples obtained from healthy dogs and cats by use of microsample blood collection tubes that contained lithium heparin provided clinically equivalent biochemical results, compared with results for samples obtained by use of standard blood collection tubes, and minimized the total sample volume collected for diagnostic testing.

Comparison of complete blood counts in samples obtained from healthy dogs and cats by use of standard and microsample blood collection tubes

2010 ◽  
Vol 237 (3) ◽  
pp. 281-287
Author(s):  
Jacqueline C. Whittemore ◽  
Bente Flatland
Keyword(s):  
Regression Analysis ◽  
Pearson Correlation ◽  
Hemoglobin Concentration ◽  
Blood Collection ◽  
Mean Corpuscular Hemoglobin ◽  
Altman Analysis ◽  
Corpuscular Hemoglobin ◽  
Bland Altman Analysis ◽  
Healthy Dogs ◽  
Blood Collection Tubes

Abstract Objective—To compare results of a CBC performed on blood samples obtained from healthy dogs and cats by use of standard and microsample collection tubes. Design—Evaluation study. Animals—29 healthy client-owned animals (14 dogs and 15 cats). Procedures—A blood sample (3 mL) was collected from each animal; 2.5 mL was transferred into a vacuum tube that contained sodium EDTA, and 0.5 mL was transferred into a microsample tube that contained sodium EDTA. Variables evaluated were total numbers of RBCs and WBCs, hemoglobin concentration, Hct, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration (MCHC), mean platelet volume, and plasma total protein concentration as well as neutrophil, lymphocyte, monocyte, eosinophil, basophil, and platelet counts. Results for the 2 types of tube in each species were compared by use of Pearson correlation coefficients, Passing-Bablok regression analysis, and Bland-Altman analysis. Results—The Pearson correlation coefficient was low for basophil count in cats and moderate, high, or very high for all other variables. Constant and proportional biases were identified for MCHC in dogs by use of Passing-Bablok regression analysis, although the mean difference between types of blood collection tubes was small. No evidence of constant or proportional bias for any other variable was revealed by regression analysis or Bland-Altman analysis. Conclusions and Clinical Relevance—Samples obtained from healthy dogs and cats by use of microsample blood collection tubes provided clinically equivalent CBC results, compared with results for samples obtained by use of standard blood collection tubes, and minimized the total sample volume collected for diagnostic testing.

scholarly journals Comparison of IOP Measurement by Goldmann Applanation Tonometer, ICare Rebound Tonometer, and Tono-Pen in Keratoconus Patients after MyoRing Implantation

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Mahmoud Rateb ◽  
Mahmoud Abdel-Radi ◽  
Zeiad Eldaly ◽  
Mohamed Nagy Elmohamady ◽  
Asaad Noor El Din
Keyword(s):  
Central Corneal Thickness ◽  
Pearson Correlation ◽  
Corneal Thickness ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Rebound Tonometer ◽  
Goldmann Applanation Tonometer ◽  
Significant Difference ◽  
Applanation Tonometer ◽  
Icare Rebound Tonometer

Purpose. To evaluate the different IOP readings by Goldmann applanation tonometer (GAT), ICare rebound tonometer, and Tono-Pen in keratoconus patients after MyoRing implantation. To assess the influence of central corneal thickness (CCT) and thinnest corneal location (TCL) on IOP measurements by different tonometers. Setting. Prospective observational study was conducted in two private centers in Egypt from February 2015 to November 2016. Methods. Seventeen eyes of 10 patients suffering from keratoconus and who underwent MyoRing implantation were recruited. All subjects underwent GAT, ICare, and Tono-Pen IOP measurements in random order. Central corneal thickness and thinnest corneal location were assessed by Pentacam. Difference in mean in IOP readings was assessed by T-test. Correlation between each pair of devices was evaluated by Pearson correlation coefficient. The Bland–Altman analysis was used to assess intertonometer agreement. Results. Seventeen eyes (10 patients) were evaluated. The mean IOP reading was 13.9 ± 3.68, 12.41 ± 2.87, and 14.29 ± 1.31 mmHg in GAT, ICare, and Tono-Pen group, respectively. There was a significant difference between IOP readings by GAT/ICare and Tono-Pen/ICare (p value: 0.032 and 0.002, respectively) with no significant difference between GAT/Tono-Pen (p value: 0.554). Mean difference in IOP measurements between GAT/ICare was 1.49 ± 2.61 mmHg, Tono-Pen/ICare was 1.89 ± 2.15 mmHg, and GAT/Tono-Pen was −0.39 ± 2.59 mmHg. There was no significant correlation between the difference in IOP readings among any pair of devices and CCC or TCL. The Bland–Altman analysis showed a reasonable agreement between any pair of tonometers.

scholarly journals Comparison of activated clotting times measured using the Hemochron Jr. Signature and Medtronic ACT Plus during cardiopulmonary bypass with acute normovolemic haemodilution

2017 ◽  
Vol 46 (2) ◽  
pp. 873-882 ◽  
Author(s):  
Jung Min Lee ◽  
Eun Young Park ◽  
Kyung Mi Kim ◽  
Jong Chan Won ◽  
Tack Koon Jung ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Cardiac Surgery ◽  
Cardiopulmonary Bypass ◽  
Bolus Dose ◽  
Blood Glucose Concentration ◽  
Altman Analysis ◽  
Clotting Time ◽  
Bland Altman Analysis ◽  
Activated Clotting Time ◽  
Protamine Administration

Objective This study compared the activated clotting time (ACT) measured using the Hemochron Jr. Signature (HACT) with the ACT measured using the Medtronic ACT Plus (MACT) during cardiopulmonary bypass (CPB) with acute normovolemic haemodilution (ANH) in patients undergoing cardiac surgery. Methods The ACT was checked at baseline with both devices after inducing anaesthesia, and 400 to 800 mL of whole blood was withdrawn to induce moderate ANH. Before initiating CPB, a 300-IU/kg bolus dose of heparin was administered to maintain the HACT at >400 s; protamine was later given to reverse the anticoagulation. The ACT was checked using both devices at baseline, during heparinisation, and after protamine administration. Results In total, 106 pairs of samples from 29 patients were analysed. The ACT showed a good correlation between the two devices (r = 0.956). However, Bland–Altman analysis showed that the MACT was higher, particularly at baseline and during heparinisation. Multiple regression analysis showed that the blood glucose concentration significantly influenced the differences between the two ACT devices. Conclusions The HACT was lower than the MACT during CPB with ANH in patients undergoing cardiac surgery. Clinicians should be cautious when using each ACT device within generally accepted reference ACT values.

scholarly journals Comparison of two methods for measurement of equine adrenocorticotropin

2017 ◽  
Vol 30 (2) ◽  
pp. 233-237 ◽  
Author(s):  
Heidi E. Banse ◽  
Nichol Schultz ◽  
Molly McCue ◽  
Ray Geor ◽  
Dianne McFarlane
Keyword(s):  
Pars Intermedia ◽  
Plasma Samples ◽  
Altman Analysis ◽  
Poor Agreement ◽  
Limits Of Agreement ◽  
Cutoff Value ◽  
Bland Altman Analysis ◽  
Acth Concentration

Accurate measurement of equine adrenocorticotropin (ACTH) is important for the diagnosis of equine pituitary pars intermedia dysfunction (PPID). Several radioimmunoassays (RIAs) and chemiluminescent immunoassays (CIAs) are used for measurement of ACTH concentration in horses; whether these methods yield similar results across a range of concentrations is not determined. We evaluated agreement between a commercial RIA and CIA. Archived plasma samples ( n = 633) were measured with both assays. Correlation between the 2 methods was moderate ( r = 0.49, p < 0.001). Bland–Altman analysis revealed poor agreement, with a proportional bias and widening limits of agreement with increasing values. Poor agreement between assays was also observed when evaluating plasma samples with concentrations at or below the recommended diagnostic cutoff value for PPID testing. The lack of agreement suggests that measurements obtained should not be considered interchangeable between methods.

scholarly journals A method comparison study of a point-of-care blood gas analyser with a laboratory auto-analyser for the determination of potassium concentrations during hyperkalaemia in patients with kidney disease

2020 ◽  
Vol 30 (3) ◽  
pp. 432-438
Author(s):  
Mogamat-Yazied Chothia ◽  
Patricia Kassum ◽  
Annalise Zemlin
Keyword(s):  
Regression Analysis ◽  
Systematic Error ◽  
Potassium Concentration ◽  
Point Of Care ◽  
Venous Blood ◽  
Mean Difference ◽  
Renal Unit ◽  
Blood Gas ◽  
Altman Analysis ◽  
Bland Altman Analysis

Introduction: Hyperkalaemia is a common electrolyte disorder that may cause life-threatening cardiac arrythmias. We aimed to determine the agreement of potassium concentrations between GEM premier 3500 point-of-care blood gas analyser (POC-BGA) and Roche Cobas 6000 c501 autoanalyser in patients with hyperkalaemia. Methods: A prospective, cross-sectional study of all consecutive adult patients referred to the Renal Unit with a serum potassium concentration ≥ 5.5 mmol/L was performed. A total of 59 paired venous blood samples were included in the final statistical analysis. Passing-Bablok regression and Bland Altman analysis were used to compare the two methods. Results: The median laboratory auto-analyser potassium concentration was 6.1 (5.9-7.1) mmol/L as compared to the POC-BGA potassium concentration of 5.7 (5.5-6.8) mmol/L with a mean difference of - 0.43 mmol/L and 95% upper and lower limits of agreement of 0.35 mmol/L and - 1.21 mmol/L, respectively. Regression analysis revealed proportional systematic error. Test for linearity did not indicate significant deviation (P = 0.297). Conclusion: Although regression analysis indicated proportional systematic error, on Bland Altman analysis, the mean difference appeared to remain relatively constant across the potassium range that was evaluated. Therefore, in patients presenting to the emergency department with a clinical suspicion of hyperkalaemia, POC-BGA potassium concentrations may be considered a surrogate for laboratory auto-analyser measurements once clinicians have been cautioned about this difference.

scholarly journals Comparison between radial artery tonometry pulse analyzer and pulsed-Doppler echocardiography derived hemodynamic parameters in cardiac surgery patients: a pilot study

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4132 ◽  
Author(s):  
Rashad Zayat ◽  
Andreas Goetzenich ◽  
Ju-Yeon Lee ◽  
HeeJung Kang ◽  
So-Hyun Jansen-Park ◽  
...  
Keyword(s):  
Pilot Study ◽  
Radial Artery ◽  
Pearson Correlation ◽  
Hemodynamic Parameters ◽  
Altman Analysis ◽  
Limits Of Agreement ◽  
Bland Altman Analysis ◽  
Number Of Patients ◽  
The Mean ◽  
Lower Limits

BackgroundBedside non-invasive techniques, such as radial artery tonometry, to estimate hemodynamic parameters have gained increased relevance as an attractive alternative and efficient method to measure hemodynamics in outpatient departments. For our pilot study, we sought to compare cardiac output (CO), and stroke volume (SV) estimated from a radial artery tonometry blood pressure pulse analyzer (BPPA) (DMP-Life, DAEYOMEDI Co., Gyeonggi-do, South Korea) to pulsed-wave Doppler (PWD) echocardiography derived parameters.MethodsFrom January 2015 to December 2016, all patients scheduled for coronary artery bypass (CABG) surgery at our department were screened. Exclusion criteria were, inter alia, moderate to severe aortic- or Mitral valve disease and peripheral arterial disease (PAD) > stage II. One hundred and seven patients were included (mean age 66.1 ± 9.9, 15 females, mean BMI 27.2 ± 4.1 kg/m2). All patients had pre-operative transthoracic echocardiography (TTE). We measured the hemodynamic parameters with the BPPA from the radial artery, randomly before or after TTE. For the comparison between the measurement methods we used the Bland-Altman test and Pearson correlation.ResultsMean TTE-CO was 5.1 ± 0.96 L/min, and the mean BPPA-CO was 5.2 ± 0.85 L/min. The Bland-Altman analysis for CO revealed a bias of −0.13 L/min and SD of 0.90 L/min with upper and lower limits of agreement of −1.91 and +1.64 L/min. The correlation of CO measurements between DMP-life and TTE was poor (r = 0.501,p < 0.0001). The mean TTE-SV was 71.3 ± 16.2 mL and the mean BPPA-SV was 73.8 ± 19.2 mL. SV measurements correlated very well between the two methods (r = 0.900,p < 0.0001). The Bland-Altman analysis for SV revealed a bias of −2.54 mL and SD of ±8.42 mL and upper and lower limits of agreement of −19.05 and +13.96 mL, respectively.ConclusionOur study shows for the first time that the DMP-life tonometry device measures SV and CO with reasonable accuracy and precision of agreement compared with TTE in preoperative cardiothoracic surgery patients. Tonometry BPPA are relatively quick and simple measuring devices, which facilitate the collection of cardiac and hemodynamic information. Further studies with a larger number of patients and with repeated measurements are in progress to test the reliability and repeatability of DMP-Life system.

scholarly journals Multicenter comparison of the Cobas 6800 system with the RealStar RT-PCR kit for the detection of SARS-CoV-2

2020 ◽  
Author(s):  
Marc Wirden ◽  
Linda Feghoul ◽  
Mélanie Bertine ◽  
Marie-Laure Nere ◽  
Quentin Le Hingrat ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression Analysis ◽  
Regression Line ◽  
Clinical Samples ◽  
Altman Analysis ◽  
Rt Pcr ◽  
Bland Altman Analysis ◽  
Deming Regression ◽  
The Mean ◽  
Pcr Assays

ABSTRACTBackgroundRT-PCR testing is crucial in the diagnostic of SARS-CoV-2 infection. The use of reliable and comparable PCR assays is a cornerstone to allow use of different PCR assays depending on the local equipment. In this work, we provide a comparison of the Cobas® (Roche) and the RealStar® assay (Altona).MethodsAssessment of the two assays was performed prospectively in three reference Parisians hospitals, using 170 clinical samples. They were tested with the Cobas® assay, selected to obtain a distribution of cycle threshold (Ct) as large as possible, and tested with the RealStar assay with three largely available extraction platforms: QIAsymphony (Qiagen), MagNAPure (Roche) and NucliSENS-easyMag (BioMérieux).ResultsOverall, the agreement (positive for at least one gene) was 76%. This rate differed considerably depending on the Cobas Ct values for gene E: below 35 (n = 91), the concordance was 99%. Regarding the positive Ct values, linear regression analysis showed a determination correlation (R2) of 0.88 and the Deming regression line revealed a strong correlation with a slope of 1.023 and an intercept of -3.9. Bland-Altman analysis showed that the mean difference (Cobas® minus RealStar®) was + 3.3 Ct, with a SD of + 2.3 Ct.ConclusionsIn this comparison, both RealStar® and Cobas® assays provided comparable qualitative results and a high correlation when both tests were positive. Discrepancies exist after 35 Ct and varied depending on the extraction system used for the RealStar® assay, probably due to a low viral load close to the detection limit of both assays.

scholarly journals The TRUTH confirmed: validation of an intraindividual comparison of gadobutrol and gadoteridol for imaging of glioblastoma using quantitative enhancement analysis

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Matthew J. Kuhn ◽  
Julia W. Patriarche ◽  
Douglas Patriarche ◽  
Miles A. Kirchin ◽  
Massimo Bona ◽  
...  
Keyword(s):  
Pearson Correlation ◽  
Linear Regression Analysis ◽  
Region Of Interest ◽  
Mean Difference ◽  
Altman Analysis ◽  
Double Blind ◽  
Bland Altman Analysis ◽  
Intraindividual Comparison ◽  
Contrast Enhanced ◽  
Magnetic Resonance Imaging Mri

Abstract Background Previous intraindividual comparative studies evaluating gadobutrol and gadoteridol for contrast-enhanced magnetic resonance imaging (MRI) of brain tumours have relied on subjective image assessment, potentially leading to misleading conclusions. We used artificial intelligence algorithms to objectively compare the enhancement achieved with these contrast agents in glioblastoma patients. Methods Twenty-seven patients from a prior study who received identical doses of 0.1 mmol/kg gadobutrol and gadoteridol (with appropriate washout in between) were evaluated. Quantitative enhancement (QE) maps of the normalised enhancement of voxels, derived from computations based on the comparison of contrast-enhanced T1-weighted images relative to the harmonised intensity on unenhanced T1-weighted images, were compared. Bland-Altman analysis, linear regression analysis and Pearson correlation coefficient (r) determination were performed to compare net QE and per-region of interest (per-ROI) average QE (net QE divided by the number of voxels). Results No significant differences were observed for comparisons performed on net QE (mean difference -24.37 ± 620.8, p = 0.840, r = 0.989) or per-ROI average QE (0.0043 ± 0.0218, p = 0.313, r = 0.958). Bland-Altman analysis revealed better per-ROI average QE for gadoteridol-enhanced MRI in 19/27 (70.4%) patients although the mean difference (0.0043) was close to zero indicating high concordance and the absence of fixed bias. Conclusions The enhancement of glioblastoma achieved with gadoteridol and gadobutrol at 0.1 mmol/kg bodyweight is similar indicating that these agents have similar contrast efficacy and can be used interchangeably, confirming the results of a prior double-blind, randomised, intraindividual, crossover study.

scholarly journals Comparison of Anterior Corneal Curvature Measurements Using a Galilei Dual Scheimpflug Analyzer and Topcon Auto Kerato-Refractometer

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Xiaogang Wang ◽  
Jing Dong ◽  
Qiang Wu
Keyword(s):  
Linear Correlation ◽  
Coefficient Of Variation ◽  
Pearson Correlation ◽  
Pearson Correlation Coefficient ◽  
Corneal Curvature ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Significant Linear Correlation ◽  
Curvature Measurements ◽  
Good Agreement

Purpose. To compare anterior corneal keratometry (K) measurements taken by a dual Scheimpflug analyzer and an auto kerato-refractometer.Methods. Sixty-four normal eyes underwent keratometric measurements with both devices. The repeatability of the auto kerato-refractometer measurements was assessed by calculating the coefficient of variation (COV). The interdevice agreement was evaluated using the Bland-Altman analysis, Pearson correlation coefficient, and paired two-tailedt-test.Results. The COV of the flatKand steepKmeasurements taken by the auto kerato-refractometer were 0.21% and 0.29%, respectively. There were no significant differences between the steepKand averageKmeasurements for the Topcon and Galilei devices (P= 0.475, andP= 0.137, resp.). The Galilei flatKvalues were lower than those of the Topcon (P= 0.002). Both of the instruments showed good agreement for all anterior corneal keratometric values. There was a significant linear correlation between the Galilei and Topcon devices for the flatK(r= 0.989,P< 0.0001), steepK(r= 0.987,P< 0.0001), and averageKvalues (r= 0.994,P< 0.0001).Conclusions. The anterior corneal flat keratometric values were not interchangeable between the Galilei and Topcon devices. However, the measurements of the two instruments showed significant linear correlation with each other.

scholarly journals Comparison of variables measured with a Scheimpflug device for evaluation of progression and detection of keratoconus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sophie Neuhann ◽  
Anna Schuh ◽  
Daniel Krause ◽  
Raffael Liegl ◽  
Valerie Schmelter ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Visual Impairment ◽  
Classification System ◽  
Staging System ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Intraclass Correlations ◽  
High Repeatability ◽  
Corneal Disorder

Abstract Keratoconus is a progressive ectatic corneal disorder, which can result in severe visual impairment. The new ABCD keratoconus classification system allows differentiated description of the disease. Aim of the study was to evaluate the components of this novel staging system (ARC, PRC, thinnest pachymetry) as well as topometric indices, deviation of normality indices, and other parameters in terms of repeatability and reliability. 317 eyes with keratoconus were examined twice with a Scheimpflug device (Pentacam, Oculus). Bland Altman analysis and intraclass correlations were carried out to evaluate the parameters repeatability and reliability. Apart from IHA (ICC = 0.730), all parameters showed excellent reliability (ICC > 0.900). ARC, PRC, thinnest pachymetry, Kmax, CKI, KI, Rmin, and Progression Avg were the best repeatable parameters with relative repeatability values < 2.5%. Other parameters performing well in terms of repeatability were IHD, ISV, IVA, and final D (RR < 13%). Regression analysis revealed consistently high repeatability along all stages of keratoconus for PRC, thinnest pachymetry, and CKI. All parameters of the ABCD staging system showed excellent reliability and repeatability, PRC and thinnest pachymetry even at all stages of keratoconus and can consequently be reliably used in the determination of keratoconus progression.

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