scholarly journals Analysis of Active Components in Salvia Miltiorrhiza Injection Based on Vascular Endothelial Cell Protection

2014 ◽  
Vol 64 (3) ◽  
pp. 325-334 ◽  
Author(s):  
Jie Shen ◽  
Kai Yang ◽  
Caihua Sun ◽  
Minxia Zheng
Keyword(s):  
Endothelial Cell ◽  
Salvia Miltiorrhiza ◽  
Antioxidant Properties ◽  
Vascular Endothelial Cell ◽  
Salvianolic Acid B ◽  
Vascular Endothelial ◽  
Active Components ◽  
Cell Protection ◽  
Salvianolic Acid ◽  
Protocatechuic Aldehyde

Abstract Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines. In this report, HPLC and measurement of antioxidant properties were used to describe the active ingredients of Salvia miltiorrhiza injection (SMI). HPLC results showed that tanshinol, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, protocatechuic acid and their metabolites in rat serum may contribute to the efficacy of SMI. Assessment of antioxidant properties indicated that differences in the composition of serum powder of SMI caused differences in vascular endothelial cell protection. When bivariate correlation was carried out it was found that salvianolic acid B, tanshinol and protocatechuic aldehyde were active components of SMI because they were correlated to antioxidant properties.

The Metabolism of Tanshinone IIA, Protocatechuic Aldehyde, Danshensu, Salvianolic Acid B and Hydroxysafflor Yellow A in Zebrafish

2020 ◽  
Vol 17 (1) ◽  
pp. 106-118
Author(s):  
Ya-Li Wang ◽  
Shi-Jun Yin ◽  
Feng-Qing Yang ◽  
Guang Hu ◽  
Guo-Can Zheng ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Tanshinone Iia ◽  
Hydroxysafflor Yellow A ◽  
Active Components ◽  
Zebrafish Model ◽  
Salvianolic Acid ◽  
Protocatechuic Aldehyde

Background: Tanshinone IIA (TIIA), protocatechuic aldehyde (PA), danshensu (DSS), salvianolic acid B (SAB) and hydroxysafflor yellow A (HSYA) are the major components of Salvia miltiorrhiza Bge. (Danshen) and Carthamus tinctorius L. (Honghua) herbal pair. These active components may contribute to the potential synergistic effects of the herbal pair. Objective: This study aimed to investigate the metabolites of TIIA, PA, DSS, SAB and HSYA in zebrafish, and to explore the influence of HSYA on the metabolism of TIIA, PA, DSS, and SAB. Method: 48 h post-fertilization zebrafish embryos were exposed either to each compound alone, TIIA (0.89 μg/mL), PA (0.41 μg/mL), DSS (0.59 μg/mL), SAB (2.15 μg/mL), and HSYA (1.83 μg/mL) and in combination with HSAY (1.83 μg/mL). The metabolites of TIIA, PA, DSS, SAB, and HSYA in zebrafish were characterized using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) and quantitatively determined by HPLC-MS with single and combined exposure. Results: Among the 26 metabolites detected and characterized from these five compounds, methylation, hydroxylation, dehydrogenation, hydrolysis, sulfation and glucuronidation were the main phase I and phase II metabolic reactions of these compounds, respectively. Furthermore, the results showed that HSYA could either enhance or reduce the amount of TIIA, PA, DSS, SAB, and their corresponding metabolites. Conclusion: The results provided a reference for the study on drug interactions in vivo. In addition, the zebrafish model which required much fewer amounts of test samples, compared to regular mammal models, had higher efficiency in predicting in vivo metabolism of compounds.

scholarly journals THROMBOMODULIN IS A PARACRINE ANTI-APOPTOTIC FACTOR FOR VASCULAR ENDOTHELIAL CELL PROTECTION DURING STRESS

2011 ◽  
Vol 57 (14) ◽  
pp. E1427
Author(s):  
Yi-Heng Li ◽  
Hsing-Chun Chung ◽  
Ting-Hsing Chao ◽  
Guey-Yueh Shi ◽  
Hua-Lin Wu
Keyword(s):  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial ◽  
Cell Protection

scholarly journals Anti-inflammatory Effect of Salvia Miltiorrhiza is Mediated via IL-6, JAK, and STAT Pathway in a Dysfunctional Vascular Endothelial Cell

2021 ◽  
Vol 8 (3) ◽  
pp. 1-12
Author(s):  
Emmanuel Adikwu Orgah
Keyword(s):  
Diabetes Mellitus ◽  
Signal Transduction ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Umbilical Vein ◽  
Salvia Miltiorrhiza ◽  
Vascular Endothelial Cell ◽  
Vascular Complication ◽  
Vascular Endothelial ◽  
Extracellular Medium

The vascular complication of diabetes mellitus is a problem for the patient, and the ability to cope with the disease and the associated inflammation is a critical aspect of diabetes. Cytokines-induced inflammation in vascular endothelial cells (VECs) plays an active role in chronic diseases such as atherosclerosis, diabetes mellitus, kidney injury, and stroke. We investigated the role of total salvianolic acids (TSA), total tanshinones (TTSN), and their combination (TSA/TTSN) on the activated vascular endothelial cell and its inhibitory effect on signal transduction and cytokines regulation. In the extracellular medium of the injury model of human umbilical vein endothelial cells (HUVECs) induced by thrombin, the human IL-6, VCAM-1, and ICAM-1 were significantly elevated (p ˂ 0.05). However, suppression in the TSA, TTSN, and TSA/TTSN (100 µg/L)-treated groups (p > 0.05) were notable. TSA alone but not TTSN and TSA/TTSN combination, inhibited the expression of P-selectin (p < 0.05) and E-selectin (p < 0.01) respectively, in VECs. Western blot analysis showed JAK and STAT expression in VECs however, the protein expression was modest in the Salvia miltiorrhiza-treated groups, indicating the potential of TSA/TTSN in the inflammatory pathways of IL-6, JAK, and STAT signal transduction in endothelial cells (ECs). This study has made novel observations regarding the components of Salvia miltiorrhiza regulatory effect on cytokines in Vascular Biology. Keywords: Atherosclerosis; Cytokines; Diabetes mellitus; HUVECs; Inflammation; Salvia militiorrhiza.

Effect of angiotensin(1-7) on the expression of E-selectin induced by angiotensinII and its mechanism in vascular endothelial cell

2010 ◽  
Vol 34 (8) ◽  
pp. S71-S71
Author(s):  
Xiaohui Shen ◽  
Zhi‑Bin Wen ◽  
Na Li ◽  
Qingmei Cheng ◽  
Xiaofan He ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Vascular Endothelial Cell ◽  
Vascular Endothelial

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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