Crude Extract of Salvia miltiorrhiza and Salvianolic Acid B Enhance in Vitro Angiogenesis in Murine SVR Endothelial Cell Line

Planta Medica ◽  
2003 ◽  
Vol 69 (01) ◽  
pp. 26-32 ◽  
Author(s):  
Ing-Shiow Lay ◽  
Jen-Hwey Chiu ◽  
Ming-Shi Shiao ◽  
Wing-Yiu Lui ◽  
Chew-Wun Wu
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Crude Extract ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Endothelial Cell Line ◽  
Salvianolic Acid ◽  
In Vitro Angiogenesis

Salvianolic acid b enhances in vitro angiogenesis and improves skin flap survival in sprague-dawley rats1

2003 ◽  
Vol 115 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Ing-Shiow Lay ◽  
Cheng-Chu Hsieh ◽  
Jen-Hwey Chiu ◽  
Ming-Shi Shiao ◽  
Wing-Yiu Lui ◽  
...  
Keyword(s):  
Skin Flap ◽  
Salvianolic Acid B ◽  
Sprague Dawley ◽  
Flap Survival ◽  
Salvianolic Acid ◽  
Skin Flap Survival ◽  
In Vitro Angiogenesis

Analysis on the Stability of Total Phenolic Acids and Salvianolic Acid B from Salvia miltiorrhiza by HPLC and HPLC-MSn

2008 ◽  
Vol 3 (5) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Man Xu ◽  
Jian Han ◽  
Hui-feng Li ◽  
Li Fan ◽  
Ai-hua Liu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Degradation Products ◽  
Biological Fluids ◽  
Salvia Miltiorrhiza ◽  
Degradation Pathway ◽  
Salvianolic Acid B ◽  
Total Phenolic ◽  
Salvianolic Acid ◽  
The Stability

The stability of salvianolic acid B and total phenolic acids from Salvia miltiorrhiza in water solutions at different temperatures, in buffered aqueous solutions at different pHs and in biological fluids, including simulated gastric and intestinal fluids, were investigated in vitro. The results showed that the degradation of salvianolic acid B was pH- and temperature-dependent. Furthermore, structures of the degradation products of salvianolic acid B and total phenolic acids were elucidated by liquid chromatography-electrospray ion trap mass spectrometry and analysis of the degraded solutions revealed seventeen degradation products. The possible degradation pathway of salvianolic acid B is proposed.

scholarly journals EA-1, a novel adhesion molecule involved in the homing of progenitor T lymphocytes to the thymus.

1991 ◽  
Vol 114 (5) ◽  
pp. 1069-1078 ◽  
Author(s):  
B A Imhof ◽  
P Ruiz ◽  
B Hesse ◽  
R Palacios ◽  
D Dunon
Keyword(s):  
Endothelial Cell ◽  
T Cell ◽  
T Lymphocytes ◽  
Cell Line ◽  
Adhesion Molecule ◽  
Newborn Mice ◽  
Embryonic Mouse ◽  
Endothelial Cell Line ◽  
Mouse Tissues

The mouse progenitor T lymphocyte (pro-T) cell line FTF1 binds in vitro to thymus blood vessels, the thymic capsule, and liver from newborn mice. A mAb, EA-1, raised against an embryonic mouse endothelial cell line, blocked adhesion. The antibody also interfered with pro-T cell adhesion to a thymus-derived mouse endothelial cell line; it had no effect on the adhesion of mature T lymphocytes and myeloid cells. The antigen recognized by EA-1 is located on the vascular endothelium of various mouse tissues and absent on pro-T cells. EA-1 antibody precipitates molecules with apparent molecular weights of 110,000, 140,000, 160,000, and 200,000. Immunoclearing and binding-inhibition studies with antibodies against known adhesion molecules suggest that the EA-1 antigen is a novel adhesion molecule involved in colonization of the embryonic thymus by T cell progenitors.

Development of immortalized human cerebromicrovascular endothelial cell line as an in vitro model of the human blood–brain barrier

1997 ◽  
Vol 11 (13) ◽  
pp. 1187-1197 ◽  
Author(s):  
Arumugam Muruganandam ◽  
Leonie Moorhouse Herx ◽  
Robert Monette ◽  
Jon P. Durkin ◽  
Danica B. Stanimirovic
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Blood Brain ◽  
Vitro Model

Effects of Phthalates on the Human Corneal Endothelial Cell Line B4G12

2012 ◽  
Vol 31 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Tanja Krüger ◽  
Yi Cao ◽  
Søren K. Kjærgaard ◽  
Lisbeth E. Knudsen ◽  
Eva C. Bonefeld-Jørgensen
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Industrial Chemicals ◽  
Cell Secretion ◽  
Endothelial Cell Line ◽  
Butyl Phthalate

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di- n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di- n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

Effect of Long-term In Vitro Lithium Exposure on mRNA Levels of Claudin-3, CYP1A1, ABCG2 and GSTM3 Genes in the hCMEC/D3 Human Brain Endothelial Cell Line

2017 ◽  
Vol 42 (6) ◽  
pp. 1013-1017 ◽  
Author(s):  
Ramzi Shawahna ◽  
Kayathiri Ganeshamoorthy ◽  
Luo Huilong ◽  
Jean-Michel Scherrmann ◽  
Pierre-Olivier Couraud ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Human Brain ◽  
Cell Line ◽  
Mrna Levels ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Line

scholarly journals Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis

2021 ◽  
Author(s):  
Jia-Ming Sun ◽  
Chia-Kang Ho ◽  
Ya Gao ◽  
Chio-Hou Chong ◽  
Dan-Ning Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graft Survival ◽  
Salvia Miltiorrhiza ◽  
Salvianolic Acid B ◽  
Fat Graft ◽  
Fat Transplantation ◽  
Salvianolic Acid ◽  
Fat Grafts

Abstract Background: Our previous study proved that Salvia miltiorrhiza could enhance fat graft survival by promoting adipogenesis. However, the effect of salvianolic acid B (Sal-B), the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza, on fat graft survival has not yet been investigated.Objective: This study aims to investigate whether salvianolic acid B could improve fat graft survival and promote preadipocyte differentiation. The underlying mechanism has also been studied.Methods: In vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2 and 4 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the adipogenesis and proliferative activities of salvianolic acid B were analyzed in cultured human adipose-derived stem cells (h-ADSCs) and 3T3-L1 cells to detect the mechanism by which salvianolic acid B affects graft survival.Results: In vivo, the weights and volumes of the fat grafts in the Sal-B-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity and more viable adipocytes were observed in the Sal-B-treated groups. In vitro, salvianolic acid B showed the ability to promote 3T3-L1 and h-ADSC proliferation and adipogenesis.Conclusions: Our in vitro experiments demonstrated that salvianolic acid B can promote the proliferation of adipose stem cells and enhance the differentiation of adipose stem cells. Simultaneously, in vivo experiments showed that salvianolic acid B can improve the survival rate of fat transplantation. Therefore, our research shed light on the potential therapeutic usage of salvianolic acid B in improving the survival rate of fat transplantation.

HIF-1 activation attenuates postischemic myocardial injury: role for heme oxygenase-1 in modulating microvascular chemokine generation

2005 ◽  
Vol 289 (2) ◽  
pp. H542-H548 ◽  
Author(s):  
Ramzi Ockaili ◽  
Ramesh Natarajan ◽  
Fadi Salloum ◽  
Bernard J. Fisher ◽  
Drew Jones ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Heme Oxygenase ◽  
Ischemia Reperfusion ◽  
Microvascular Endothelial Cell ◽  
Endothelial Cell Line ◽  
Human Microvascular Endothelial Cell ◽  
Microvascular Endothelial

The CXC chemokine IL-8, which promotes adhesion, activation, and transmigration of polymorphonuclear neutrophils (PMN), has been associated with production of tissue injury in reperfused myocardium. Hypoxia-inducible factor-1 (HIF-1) is a heterodimeric peptide that is a key regulator of genes such as heme oxygenase (HO)-1 expressed under hypoxic conditions. We hypothesized that HO-1 plays an important role in regulating proinflammatory mediator production under conditions of ischemia-reperfusion. HIF-1 was activated in the human microvascular endothelial cell line (HMEC-1) with the prolyl hydroxylase inhibitor dimethyloxalylglycine (DMOG). DMOG significantly attenuated cytokine-induced IL-8 promoter activity and protein secretion and cytokine-induced PMN migration across human microvascular endothelial cell line HMEC-1 monolayers. In vivo studies in a rabbit model of myocardial ischemia-reperfusion showed that rabbits pretreated with a 20 mg/kg DMOG infusion ( n = 6) 24 h before study exhibited a 21.58 ± 1.76% infarct size compared with 35.25 ± 2.06% in saline-treated ischemia-reperfusion animals ( n = 6, change in reduction = 39%; P < 0.001). In DMOG-pretreated (20 mg/kg) animals, plasma IL-8 levels at 3 h after onset of reperfusion were 405 ± 40 pg/ml vs. 790 ± 40 pg/ml in saline-treated ischemia-reperfusion animals ( P < 0.001). DMOG pretreatment reduced myocardial myeloperoxidase activity, expressed as number of PMN per gram of myocardium, to 1.43 ± 0.59 vs. 4.86 ± 1.1 ( P = 0.012) in saline-treated ischemia-reperfused hearts. Both in vitro and in vivo DMOG-attenuated IL-8 production was associated with robust HO-1 expression. Thus our data show that HIF-1 activation induces substantial HO-1 expression that is associated with attenuated proinflammatory chemokine production by microvascular endothelium in vitro and in vivo.

Bioactive Cycloartane-Type Triterpene Glycosides from Astragalus elongatus

2008 ◽  
Vol 63 (11-12) ◽  
pp. 813-820 ◽  
Author(s):  
İhsan Çalış ◽  
Matej Barbič ◽  
Guido Jürgenliemk
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
2D Nmr ◽  
Microvascular Endothelial Cell ◽  
Inhibition Of Proliferation ◽  
Endothelial Cell Line ◽  
Human Microvascular Endothelial Cell ◽  
Esi Ms ◽  
Weak Activity

Abstract Together with two known cycloartane-type glycosides, askendosides D (3-O-[α-arabinopyranosyl-( 1→2)-β-xylopyranosyl]-6-O-β-xylopyranosyl-cycloastragenol, 2) and G (3-O- [α-arabinopyranosyl-(1→2)-β-xylopyranosyl]-16-O-β-glucopyranosyl-3β,6α,16β,24(R),25- pentahydroxycycloartane, 3), also a new monodesmosidic cycloartane-type glycoside, elongatoside (1), was isolated from the roots of Astragalus elongatus and identified as 3-O- [α-arabinopyranosyl-(1→2)-β-xylopyranosyl]-cycloastragenol. All structures were unambiguously determined by means of spectroscopic and spectrometric methods (1D and 2D NMR, ESI-MS). The isolated compounds were tested for the inhibition of proliferation and ICAM-1 expression in vitro using the human microvascular endothelial cell line (HMEC-1). 1 showed weak activity in the ICAM-1 assay.

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