scholarly journals Low-temperature plasma applications in chemical fungicide treatment reduction

2020 ◽  
Vol 13 (1) ◽  
pp. 26-33
Author(s):  
Lucia Hoppanová ◽  
Veronika Medvecká ◽  
Juliana Dylíková ◽  
Daniela Hudecová ◽  
Barbora Kaliňáková ◽  
...  

AbstractIn order to reduce the environmental burden of chemicals, various new alternatives to seed protection are being sought. Our aim was to find an environmentally acceptable solution leading to the inactivation of seed-borne phytopathogenic fungi Fusarium culmorum on the surface of wheat and barley seeds with a positive effect on their germination. As a low-temperature plasma (LTP) source, a Diffuse Coplanar Surface Barrier Discharge (DCSBD) was used. Plasma generated by DCSBD is non-equilibrium, cold, diffuse, macroscopically homogeneous even in ambient air at atmospheric pressure. Experimental results showed that LTP treatment in the range of 120—300 s significantly inhibits the growth of F. culmorum on the surface of the seeds. The efficiency of LTP treatment was compared with traditional seed protection processes using chemical fungicide and also with combined seed pretreatment by plasma and subsequent application of chemical fungicide. No growth of F. culmorum was observed after the combination of Vitavax 2000 fungicide application in the dose of 10 % and 60 s of LTP treatment even on the 5th day of incubation. Better wettability of seeds with the chemical fungicide was related to the change on seed surface, which becomes hydrophilic after 10 s of LTP application. Short LTP exposure times did not affect germination and improved the growth parameter of cereal seeds. By combining physical (LTP) and chemical (Vitavax 2000) treatments of cereal seeds, it is possible to effectively reduce the required amount of chemical fungicide and to stimulate germination and early growth seed parameters.

Biologia ◽  
2012 ◽  
Vol 67 (3) ◽  
Author(s):  
Mária Henselová ◽  
Ľudmila Slováková ◽  
Michal Martinka ◽  
Anna Zahoranová

AbstractThe seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.


2019 ◽  
Vol 23 (3) ◽  
pp. 746-754
Author(s):  
Dinar Dilshatovich Fazullin ◽  
Gennady Vitalievich Mavrin ◽  
Vladislav Olegovich Dryakhlov ◽  
Ildar Gilmanovich Shaikhiev ◽  
Irek Rashatovich Nizameyev

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 194
Author(s):  
Joanna Pawłat ◽  
Piotr Terebun ◽  
Michał Kwiatkowski ◽  
Katarzyna Wolny-Koładka

Sterilization of municipal waste for a raw material for the production of refuse-derived fuel and to protect surface and ground waters against biological contamination during transfer and storage creates a lot of problems. This paper evaluates the antimicrobial potential of non-equilibrium plasma in relation to the selected groups of microorganisms found in humid waste. The proposed research is to determine whether mixed municipal waste used for the production of alternative fuels can be sterilized effectively using low-temperature plasma generated in a gliding arc discharge reactor in order to prevent water contamination and health risk for working staff. This work assesses whether plasma treatment of raw materials in several process variants effectively eliminates or reduces the number of selected groups of microorganisms living in mixed municipal waste. The presence of vegetative bacteria and endospores, mold fungi, actinobacteria Escherichia coli, and facultative pathogens, i.e., Staphylococcus spp., Salmonella spp., Shigella spp., Enterococcus faecalis and Clostridium perfringens in the tested material was microbiologically analyzed. It was found that the plasma treatment differently contributes to the elimination of various kinds of microorganisms in the analyzed raw materials. The effectiveness of sterilization depended mainly on the time of raw materials contact with low-temperature plasma. The results are very promising and require further research to optimize the proposed hygienization process.


Sign in / Sign up

Export Citation Format

Share Document