Effects of butylparaben on antioxidant enzyme activities and histopathological changes in rat tissues

AbstractButyl p-hydroxybenzoic acid, also known as butylparaben (BP), is one of the most common parabens absorbed by the skin and gastrointestinal tract and metabolised in the liver and kidney. Recent in vivo and in vitro studies have raised concern that BP causes reproductive, development, and teratogenic toxicity. However, BP-induced oxidative stress and its relation to tissue damage has not been widely investigated before. Therefore, we aimed to investigate the effects of butyl 4-hydroxybenzoate on enzyme activities related to the pentose phosphate pathway and on glutathione-dependent enzymes such as glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) in kidney, liver, brain, and testis tissues. Male rats were randomly divided into four groups to orally receive corn oil (control) or 200, 400, or 800 mg/kg/day of BP for 14 days. Then we measured G6PD, GR, GST, 6-PGD, and GPx enzyme activities in these tissues and studied histopathological changes. BP treatment caused imbalance in antioxidant enzyme activities and tissue damage in the liver, kidney, brain, and testis. These findings are the first to show the degenerative role of BP on the cellular level. The observed impairment of equivalent homeostasis and antioxidant defence points to oxidative stress as a mechanism behind tissue damage caused by BP.

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Potential protective effects of the edible alga Arthrospira platensis against lead-induced oxidative stress, anemia, kidney injury, and histopathological changes in adult rats

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2018-0428 ◽

2019 ◽

Vol 44 (3) ◽

pp. 271-281 ◽

Cited By ~ 5

Author(s):

Manel Gargouri ◽

Ahlem Soussi ◽

Amel Akrouti ◽

Christian Magné ◽

Abdelfattah El Feki

Keyword(s):

Oxidative Stress ◽

Uric Acid ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Hematological Parameters ◽

Kidney Injury ◽

Arthrospira Platensis ◽

Male Rats ◽

Antioxidant Enzyme Activities ◽

Protein Carbonyl Content

Oxidative damage has been proposed as a possible mechanism involved in lead toxicity. This study investigated the possible protective effect of dietary Arthrospira platensis supplementation against lead acetate-induced kidney injury in adult male rats. Rats were divided into 4 groups: normal rats (control rats), rats treated with spirulina, rats treated with lead (Pb) (0.344 g/kg body weight), and rats treated with Pb and spirulina. The exposure of rats to Pb for 30 days provoked renal damage with significant increases in hematological parameters, oxidative stress-related parameters (i.e., thiobarbituric acid reactive substances, protein carbonyl content, advanced oxidation protein products, and hydrogen peroxide), creatinine and urea levels in plasma, and uric acid level in urine. Conversely, antioxidant enzyme activities (i.e., catalase, glutathione peroxidase, and superoxide dismutase) and levels of nonprotein thiols, plasma uric acid, and urinary creatinine and urea decreased. The administration of spirulina to Pb-treated rats significantly improved weight, peripheral blood parameters, oxidative stress-related parameters, renal biomarker levels, and antioxidant enzyme activities. Also, rats treated with Pb and spirulina had normal kidney histology. These healing effects are likely the result of the high phenol content and significant antioxidant capacity of A. platensis. Our data strongly suggest that spirulina supplementation improves kidney function and plays an important role in the prevention of complications of Pb intoxication.

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Diverse age-related effects of Bacopa monnieri and donepezil in vitro on cytokine production, antioxidant enzyme activities, and intracellular targets in splenocytes of F344 male rats

International Immunopharmacology ◽

10.1016/j.intimp.2012.11.018 ◽

2013 ◽

Vol 15 (2) ◽

pp. 260-274 ◽

Cited By ~ 12

Author(s):

Hannah P. Priyanka ◽

Ran Vijay Singh ◽

Miti Mishra ◽

Srinivasan ThyagaRajan

Keyword(s):

Antioxidant Enzyme ◽

Enzyme Activities ◽

Cytokine Production ◽

Bacopa Monnieri ◽

Male Rats ◽

Antioxidant Enzyme Activities ◽

Age Related ◽

Intracellular Targets

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Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

Molecules ◽

10.3390/molecules25194426 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4426

Author(s):

Roberta Fusco ◽

Marika Cordaro ◽

Rosalba Siracusa ◽

Alessio Filippo Peritore ◽

Enrico Gugliandolo ◽

...

Keyword(s):

Oxidative Stress ◽

Superior Mesenteric Artery ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Mesenteric Artery ◽

Ischemia Reperfusion ◽

Anacardium Occidentale ◽

Male Rats ◽

Antioxidant Enzyme Activities ◽

Myeloperoxidase Activity

Ischemia/reperfusion injury is a severe disorder associated with a high mortality. Several antioxidant and pharmacological properties of cashew nuts (Anacardium occidentale L.) and its metabolites from different countries have recently been described. It is a medicinal plant with important therapeutic effects. This study aimed to verify the effect of an oral administration of cashew nuts in a rat model of ischemia/reperfusion (I/R). Adult male rats were subjected to intestinal I/R injury by clamping the superior mesenteric artery for 30 min and then allowing animals to 1 h of reperfusion. Rats subjected to I/R of the gut showed a significant increase in different biochemical markers. In particular, we evaluated lipid peroxidation, tissue myeloperoxidase activity, protein carbonyl content, reactive oxygen species generation and decreased antioxidant enzyme activities. Western blot analysis showed the activation of the NRF2 and NF-kB pathways. Increased immunoreactivity to nitrotyrosine, PARP, P-selectin, and ICAM-1 was observed in the ileum of rats subjected to I/R. Administration of cashew nuts (100 mg/kg) significantly reduced the mortality rate, the fall in arterial blood pressure, and oxidative stress and restored the antioxidant enzyme activities by a mechanism involving both NRF2 and NF-kB pathways. Cashew nuts treatments reduced cytokines plasma levels, nitrotyrosine, and PARP expression as well as adhesion molecules expressions. Additionally, cashew nuts decreased the intestinal barrier dysfunction and mucosal damage, the translocation of toxins and bacteria, which leads to systemic inflammation and associated organs injuries in particular of liver and kidney. Our study demonstrates that cashew nuts administration exerts antioxidant and pharmacological protective effects in superior mesenteric artery occlusion–reperfusion shock.

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Zika virus induces oxidative stress and decreases antioxidant enzyme activities in vitro and in vivo

Virus Research ◽

10.1016/j.virusres.2020.198084 ◽

2020 ◽

Vol 286 ◽

pp. 198084 ◽

Cited By ~ 1

Author(s):

Letícia Trindade Almeida ◽

Ariane Coelho Ferraz ◽

Camila Carla da Silva Caetano ◽

Marília Bueno da Silva Menegatto ◽

Ana Cláudia dos Santos Pereira Andrade ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzyme ◽

Zika Virus ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities

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Effects of prolonged stanozolol treatment on antioxidant enzyme activities, oxidative stress markers, and heat shock protein HSP72 levels in rat liver

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/j.jsbmb.2003.09.001 ◽

2003 ◽

Vol 87 (4-5) ◽

pp. 269-277 ◽

Cited By ~ 40

Author(s):

Angel Pey ◽

Ana Saborido ◽

Isabel Blázquez ◽

Jerónimo Delgado ◽

Alicia Megı́as

Keyword(s):

Oxidative Stress ◽

Heat Shock ◽

Heat Shock Protein ◽

Antioxidant Enzyme ◽

Rat Liver ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Oxidative Stress Markers ◽

Stress Markers

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Effects of Cadmium on Growth, Oxidative Stress and Antioxidant Enzyme Activities in Peanut (Arachis hypogaea L.) Seedlings

Journal of Agricultural Science ◽

10.5539/jas.v4n6p142 ◽

2012 ◽

Vol 4 (6) ◽

Cited By ~ 1

Author(s):

Shihua Shan ◽

Feng Liu ◽

Chunjuan Chunjuan Li ◽

Shubo Wan

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzyme ◽

Arachis Hypogaea ◽

Enzyme Activities ◽

Antioxidant Enzyme Activities ◽

Arachis Hypogaea L

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Antioxidants and selenocompounds inhibit 3,5-dimethylaminophenol toxicity to human urothelial cells

Archives of Industrial Hygiene and Toxicology ◽

10.2478/aiht-2019-70-3159 ◽

2019 ◽

Vol 70 (1) ◽

pp. 18-29 ◽

Cited By ~ 4

Author(s):

Pinar Erkekoglu ◽

Ming-Wei Chao ◽

Chia-Yi Tseng ◽

Bevin P. Engelward ◽

Ozge Kose ◽

...

Keyword(s):

Oxidative Stress ◽

Bladder Cancer ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Mammalian Cells ◽

Antioxidant Enzyme Activities ◽

Urothelial Cells ◽

Dna Breaks ◽

Primary Metabolite ◽

Stress Changes

AbstractExposure to alkyl anilines may lead to bladder cancer, which is the second most frequent cancer of the urogenital tract. 3,5-dimethylaniline is highly used in industry. Studies on its primary metabolite 3,5-dimethylaminophenol (3,5-DMAP) showed that this compound causes oxidative stress, changes antioxidant enzyme activities, and leads to death of different mammalian cells. However, there is no in vitro study to show the direct effects of 3,5-DMAP on human bladder and urothelial cells. Selenocompounds are suggested to decrease oxidative stress caused by some chemicals, and selenium supplementation was shown to reduce the risk of bladder cancer. The main aim of this study was to investigate whether selenocompounds organic selenomethionine (SM, 10 µmol/L) or inorganic sodium selenite (SS, 30 nmol/L) could reduce oxidative stress, DNA damage, and apoptosis in UROtsa cells exposed to 3,5-DMAP. 3,5-DMAP caused a dose-dependent increase in intracellular generation of reactive oxygen species, and its dose of 50 µmol/L caused lipid peroxidation, protein oxidation, and changes in antioxidant enzyme activities in different cellular fractions. The comet assay also showed single-strand DNA breaks induced by the 3,5-DMAP dose of 50 µmol/L, but no changes in double-strand DNA breaks. Apoptosis was also triggered. Both selenocompounds provided partial protection against the cellular toxicity of 3,5-DMAP. Low selenium status along with exposure to alkyl anilines can be a major factor in the development of bladder cancer. More mechanistic studies are needed to specify the role of selenium in bladder cancer.

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