scholarly journals In vivo and in vitro hepatoprotective effect of three endemic plants against carbon tetrachloride-induced liver damage in rats

2021 ◽  
Vol 8 (1) ◽  
pp. 15-36
Author(s):  
Houria Boubellouta ◽  
Fatima Khelifi Touhami ◽  
Djahida Mahdi
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Dose Dependent ◽  
Β Carotene

Abstract The aim of the study was to investigate in vitro potential antioxidant and hepatoprotective effects of methanolic extracts from the aerial parts of Matricaria pubescens, Centaurea incana, and Santolina africana, against carbon tetrachloride induced liver damage in rats. The different aerial parts of three methanolic extracts, exhibited antioxidant activity in the DPPH free radical (IC50, 0.032, 0.087 and 0.044 mg/ml) respectively. The reducing ability of the extracts showed a dose-dependent trend increasing with increase in the concentrations of the extracts.The antioxidant activity of samples at the concentration of 2 mg/ml was reflected in their ability to inhibit the bleaching of β-carotene. Treated groups with extracts of studied three plants showed decrease in plasma levels of AST, ALT, ALP, and an increase in plasma catalase levels activities, while hepatic MDA decreased and catalase increased as compared to carbon tetrachloride treated groups. In consistency with in vivo and in vitro results, extracts of all three plants ameliorated the carbon tetrachloride extensive damage to liver tissue of treated animals, but, Santolina africana extract was the most efficient.

Antioxidant and hepatoprotective effects of a pigment–protein complex from Chlorella vulgaris on carbon tetrachloride-induced liver damage in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (116) ◽  
pp. 96097-96104 ◽  
Author(s):  
Xixi Cai ◽  
Qian Yang ◽  
Shaoyun Wang
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Chlorella Vulgaris ◽  
Protein Complex ◽  
Protective Action ◽  
Hepatoprotective Effects ◽  
Pigment Protein Complex

A pigment–protein complex isolated from Chlorella exhibited significant antioxidant activity in vitro and manifested discernible protective action in CCl4-induced hepatotoxicity in vivo.

Antioxidative and Hepatoprotective Effects of Rubus canescens DC. growing wild in Lebanon

2019 ◽  
Vol 09 ◽  
Author(s):  
Ghaydaa Mohamad Salim ◽  
Mohammad H. El-Dakdouki ◽  
Hiba Abdallah ◽  
Hatem M. Nasser ◽  
Nelly Arnold-Apostolides
Keyword(s):  
Antioxidant Activity ◽  
Histological Analysis ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methanolic Extracts ◽  
Phytochemical Profile ◽  
Dpph Radical Scavenging ◽  
Β Carotene

Background: Plants of the genus Rubus (family Rosaceae) have been used for diverse medicinal purposes for centuries. We hypothesized that the scarcely investigated R. canescens DC. should, like other species of the genus Rubus, exhibit prominent antioxidant activity. Objective: The present study investigates the in vitro and in vivo antioxidant activity of fruit juice as well as aqueous and methanolic extracts of the areal parts of Rubus canescens DC., and decipher the phytochemical profile of the methanolic extracts by GC-MS. Methods: The in vitro antioxidant activity was assessed by DPPH radical scavenging assay and β-carotene bleaching assays. The in vivo antioxidant activity of the extracts was assessed by measuring ALT, AST, CAT, and SOD levels in CCl4-challenged mice in two experimental models (chronic exposure and preventive). Histological analysis was conducted on H&E stained liver sections, and the phytochemical profile of methanolic extracts was investigated by GC-MS. Results: DPPH radical scavenging assay revealed that the methanolic leaves extract exhibited the highest activity, while the juice was the most active in the β-carotene bleaching assay. The in vivo experiments suggested that the extracts have promising antioxidant potential and hepatoprotective effect capable of promoting liver functions. Histological analysis of liver sections revealed that administrating juice extract regenerated hepatocytes while reducing inflammation. GC-MS analysis indicated the presence of squalene, β-amyrin, and γ-sitosterol that may have contributed to the observed activity. Conclusion: The current study provided the first in vivo evidence supporting the antioxidative and hepatoprotective effects of R. canescens DC. growing wild in Lebanon.

scholarly journals Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

2020 ◽  
Author(s):  
Gedefaw Getnet Amare ◽  
Tadesse Awgichew ◽  
Solomon Ahmed ◽  
Zemene Demelash Kifle
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antimalarial Activity ◽  
Potential Effect ◽  
Pack Cell Volume ◽  
Curative Effect ◽  
Parasitemia Level ◽  
Dose Dependent

Abstract Background: Nature has gifted a variety of plants having potential effect against plasmodium parasites. The present study was aimed to determine in vitro and in vivo antimalarial activity of the leaf latex of Aloe weloensis.Methods: In vitro antimalarial activity of the leaf latex of A. weloensis was determined against 3D7 strain of P. falciparum. Antimalarial activity of the three doses the latex was evaluated in 4 day-suppressive and curative models against P. berghei infected mice. Antioxidant activity of the leaf latex of A. weloensis was assessed in 2,2- diphenyl 1- picrylhydrazine assay model. Results: Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 400 μg/mL (73.54%). The leaf latex of A. weloensis was demonstrated inhibitory activity against 3D7 malarial strain (IC50 = 9.14 μg/ml). Suppressive and curative effect of the latex was found to be dose dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in 4-day suppressive test compared to vehicle control. Parasitemia level of the mice treated with 200, 400 and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58% and 64% respectively in curative test. Administration of the leaf latex of A. weloensis significantly (p<0.01) improved mean survival time, pack cell volume, rectal temperature and body weight of P. berghei infected mice. Conclusion: The finding showed that the leaf latex of Aloe weloensis endowed prominent antimalarial and antioxidant activities. The result can serve as a step towards the development of safe and effective herbal therapy against plasmodium parasites.

scholarly journals Phenolic Content, Antioxidant Activity, Anti-Inflammatory Potential, and Acute Toxicity Study of Thymus leptobotrys Murb. Extracts

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Asmaa Oubihi ◽  
Hanae Hosni ◽  
Issmail Nounah ◽  
Abdessamad Ettouil ◽  
Hicham Harhar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Female Rats ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Control Group ◽  
Standard Drug ◽  
Methanolic Extracts ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Thymus leptobotrys is a medicinal plant belonging to the Lamiaceae family, endemic in Morocco, and used in traditional medicine. The present work aims to study the phenolic compounds, the antioxidant activity, the anti-inflammatory effect, and the toxicity of two ethanolic and methanolic extracts of Thymus leptobotrys aerial part. The yield of the methanolic extraction (22.2%) is higher than that of the ethanolic extraction (15.8%) and is characterized by higher contents of polyphenols 243.08 mg/g GAE (mg/g of gallic acid), flavonoids 179.28 mg/g RE (mg/g of rutin), and tannins 39.31 mg/g CE (mg/g of catechin). The in vitro measurement of antioxidant activity with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical reduction test and Trolox equivalent antioxidant capacity (TEAC) test demonstrates the higher performance of the methanolic extract. The evaluation of the anti-inflammatory effect in vivo on adult Wistar female rats leads to a very significant decrease in the inflammation of the edema compared to the standard drug (indomethacin) and the control group. The toxicity test reveals that both extracts showed no toxicity within an LD50 above 2000 mg/kg body weight of the rats.

Estimation of total Phenolic and ϑlavonoids content and Studies on antioxidant activity of different extracts of Olax scandens by FRAP assay

2020 ◽  
Vol 11 (3) ◽  
pp. 3367-3371
Author(s):  
Pranaya P ◽  
Akiladevi D
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Frap Assay ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Ic50 Value

Olax scandens Roxb. (family Olacaceae) available in throughout tropical India. The current study, aerial parts of different concentrates of Olax scandens was evaluated for its in-vitro antioxidant potential by FRAP assay taking ascorbic acid as the standard and estimation of total phenolic content and flavonoids content. The IC50 value was originated that methanolic concentrates of Olax scandens are more efficient in antioxidant activity by FRAP methods compared EA & PE concentrates. The methanolic concentrates of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 207µg/ml & 50µg/ml by Ferric reducing ability Power assay. The methanolic and EA concentrates of Olax scandens showed the total phenolic content (14.426 ± 0.032, 4.128 ± 0.025) respectively, and ৒lavonoids content (11.526 ± 0.054, 3.682 ± 0.042) respectively. Invitro antioxidant studies show methanolic concentrates of Olax scandens have better antioxidant activity as well as a higher content of total phenolic and flavonoids content. These results indicate that aerial parts of methanolic concentrates Olax scandens could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

scholarly journals Effect of Alcoholic Extracts of Cymbopogon citratus upon the Control of Colletotrichum gloeosporioides in vitro and upon the Post-harvest Quality of Guavas

2019 ◽  
pp. 1-8
Author(s):  
Ana P. F. A. Santos ◽  
Amanda P. Mattos ◽  
Adriana T. Itako ◽  
João B. Tolentino Júnior ◽  
Gabriela S. Moura ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Post Harvest ◽  
Methanolic Extracts ◽  
Dose Dependent

Aims: This work aimed at evaluating the effects of ethanolic and methanolic extracts of lemongrass upon the control in vitro of Colletotrichum gloeosporioides and upon the post-harvest quality of guavas “Paluma”. Methodology: We analyzed the inhibition of mycelial growth and sporulation of the pathogen at different concentrations of the extracts (8%; 5%; 3%; 1.5% and 0.5%). In the post-harvest assay, the guavas were treated by immersion in distilled water, ethanolic and methanolic extracts (1%; 0.5% and 0.25%) and stored at 25ºC ± 2ºC for eight days. We evaluated mass loss, total soluble solids, total titratable acidity, ratio, reducing and non-reducing sugars, ascorbic acid and pH and the incidence of anthracnose. Results: In the test in vitro, the pathogen growth inhibition was dose-dependent and the sporulation was completely inhibited upon higher concentrations of extract. At post-harvest, the fruits maintained their physicochemical characteristics, and the treatments were not efficient at retarding fruit ripening. Although the tested treatments inhibited the plant pathogen C. gloesporioides in vitro, they were not efficient at controlling the disease in vivo. Conclusion: The extracts showed control in vitro of C. gloeosporioides at 8%. However, the extracts were not effective at controlling the disease after harvest.

scholarly journals Anti-malarial activity of the leaf latex of Aloe weloensis (Aloaceae) against plasmodium parasites 

2021 ◽  
Author(s):  
Gedefaw Getnet Amare ◽  
Amsalu Degu ◽  
Zemene Demelash Kifle
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Inhibition Activity ◽  
Survival Times ◽  
Dpph Assay ◽  
Curative Effect ◽  
Parasitemia Level ◽  
Dose Dependent

Abstract Background: Lack of available vaccines and emerging resistance on the anti-malarial drug have provided the necessity to find noble plant--based anti-malarial drugs. The leaf latex Aloe weloensis has been used in folk medicine against malarial and other human ailments in Ethiopia. Hence, the present study aimed to investigate the anti-malarial activity of the leaf latex of A. weloensis against Plasmodium parasites to validate its traditional claim.Methods: The leaf latex of A. weloensis was evaluated in vitro anti-malarial activity against 3D7 strain of Plasmodium falciparum. The prophylactic and curative models were employed to determine in vivo anti-malarial activity of the latex against P. berghei infected mice, and antioxidant activity of the leaf latex of A. weloensis was assessed in DPPH assay.Results: The leaf latex of Aloe weloensis endowed with free radical inhibition activity (IC50 = 10.25 μg/ml). The latex of A. weloensis leaf was demonstrated inhibitory activity against 3D7 strain of P. falciparum (IC50 = 9.14 μg/ml). The prophylactic and curative effect of the latex was found to be dose-dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in prophylactic test compared to the control. Parasitemia level of the mice treated with 200, 400, and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58%, and 74% respectively in the curative test. The leaf latex significantly (p<0.01) improved mean survival times, packed cell volume , rectal temperature, and bodyweight of P. berghei infected mice.Conclusion: The result was confirmed the anti-malarial activity of the leaf latex of Aloe weloensis at various doses which corroborates the traditional uses of the plant.

scholarly journals Evaluation of the antioxidant potentials of extract and fractions of Vitex doniana fruit and identification of the bioactive metabolites using HPLC-DAD-MS analysis

2021 ◽  
Vol 17 (1) ◽  
pp. 160-175
Author(s):  
Keziah Uchechi Ajah ◽  
Ozioma Anne Asomugha ◽  
Chinazom Perpetua Ifedigbo ◽  
Kosoluchukwu Chidubem Umeh ◽  
Onyeka Chinwuba Obidiegwu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Vitex Doniana

Isoflavones and flavonoids in general found in fruits and vegetable act as natural antioxidants. This study was aimed at screening the isoflavone-rich fractions of Vitex doniana fruit extract for antioxidant activity using in vitro and in vivo models. The ethyl acetate and butanol fractions were screened for in vitro antioxidant activity by DPPH free radical scavenging and FRAP (Fe3+ Reducing Antioxidant Power) assays using ascorbic acid as standard. In vivo antioxidant activity was also tested against carbon tetrachloride-induced toxicity in mice using carbon tetrachloride (4 mL/kg) and silymarin (100 mg/kg) as standard. The ethyl acetate fraction and butanol Vacuum Liquid Chromatography (VLC) sub-fractions were subjected to High Performance Liquid Chromatography–Diode Array Detector (HPLC-DAD) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The ethyl acetate and butanol fractions inhibited DPPH radicals with an IC50 of 897 µg/mL and 2809.38 µg/mL respectively. Both fractions also showed mild FRAP result with EC50 of 1401 µg/mL and 7051 µg/mL respectively. The fractions produced significant decrease (P < 0.05) in ALP levels only while ethyl acetate fraction caused a significant reduction in the value of only malondialdehyde. Both fractions also increased levels of superoxide dismutase (SOD). HPLC-DAD analysis led to the detection of cinnamic acid, protocatechuic acid and two isoflavones daidzein and genistein. Further LC-MS analysis confirmed the presence of the isoflavones tectorigenin, 5-O-methylgenistein, and 5-O-methyltectorigenin, among other flavonoids, peonidin, 5-methylpeonidin, methylmalvidin and kaempferol-3-O-(2''galloyl)-glucopyranoside. The detected isoflavones and other flavonoids may contribute to the observed mild in vitro and good in vivo antioxidant activity of V. doniana fruit extract and fractions.

scholarly journals Anti-malarial activity of the leaf latex of Aloe weloensis (Aloaceae) against plasmodium parasites 

2021 ◽  
Author(s):  
Gedefaw Getnet Amare ◽  
Amsalu Degu ◽  
Zemene Demelash Kifle
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Inhibition Activity ◽  
Survival Times ◽  
Dpph Assay ◽  
Curative Effect ◽  
Parasitemia Level ◽  
Dose Dependent

Abstract Lack of available vaccines and emerging resistance on the anti-malarial drug have provided the necessity to find noble plant--based anti-malarial drugs. The leaf latex Aloe weloensis has been used in folk medicine against malarial and other human ailments in Ethiopia. Hence, the present study aimed to investigate the anti-malarial activity of the leaf latex of A. weloensis against Plasmodium parasites to validate its traditional claim. Methods: The leaf latex of A. weloensis was evaluated in vitro anti-malarial activity against 3D7 strain of Plasmodium falciparum. The prophylactic and curative models were employed to determine in vivo anti-malarial activity of the latex against P. berghei infected mice, and antioxidant activity of the leaf latex of A. weloensis was assessed in DPPH assay. Results: The leaf latex of Aloe weloensis endowed with free radical inhibition activity (IC50 = 10.25 μg/ml). The latex of A. weloensis leaf was demonstrated inhibitory activity against 3D7 strain of P. falciparum (IC50 = 9.14 μg/ml). The prophylactic and curative effect of the latex was found to be dose-dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in prophylactic test compared to the control. Parasitemia level of the mice treated with 200, 400, and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58%, and 74% respectively in the curative test. The leaf latex significantly (p<0.01) improved mean survival times, packed cell volume , rectal temperature, and bodyweight of P. berghei infected mice. Conclusion: The result was confirmed the anti-malarial activity of the leaf latex of Aloe weloensis at various doses which corroborates the traditional uses of the plant.

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