Antioxidant and hepatoprotective effects of a pigment–protein complex from Chlorella vulgaris on carbon tetrachloride-induced liver damage in vivo

RSC Advances ◽  
2015 ◽  
Vol 5 (116) ◽  
pp. 96097-96104 ◽  
Author(s):  
Xixi Cai ◽  
Qian Yang ◽  
Shaoyun Wang
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Chlorella Vulgaris ◽  
Protein Complex ◽  
Protective Action ◽  
Hepatoprotective Effects ◽  
Pigment Protein Complex

A pigment–protein complex isolated from Chlorella exhibited significant antioxidant activity in vitro and manifested discernible protective action in CCl4-induced hepatotoxicity in vivo.

scholarly journals In vivo and in vitro hepatoprotective effect of three endemic plants against carbon tetrachloride-induced liver damage in rats

2021 ◽  
Vol 8 (1) ◽  
pp. 15-36
Author(s):  
Houria Boubellouta ◽  
Fatima Khelifi Touhami ◽  
Djahida Mahdi
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Damage ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Reducing Ability ◽  
Dose Dependent ◽  
Β Carotene

Abstract The aim of the study was to investigate in vitro potential antioxidant and hepatoprotective effects of methanolic extracts from the aerial parts of Matricaria pubescens, Centaurea incana, and Santolina africana, against carbon tetrachloride induced liver damage in rats. The different aerial parts of three methanolic extracts, exhibited antioxidant activity in the DPPH free radical (IC50, 0.032, 0.087 and 0.044 mg/ml) respectively. The reducing ability of the extracts showed a dose-dependent trend increasing with increase in the concentrations of the extracts.The antioxidant activity of samples at the concentration of 2 mg/ml was reflected in their ability to inhibit the bleaching of β-carotene. Treated groups with extracts of studied three plants showed decrease in plasma levels of AST, ALT, ALP, and an increase in plasma catalase levels activities, while hepatic MDA decreased and catalase increased as compared to carbon tetrachloride treated groups. In consistency with in vivo and in vitro results, extracts of all three plants ameliorated the carbon tetrachloride extensive damage to liver tissue of treated animals, but, Santolina africana extract was the most efficient.

Hepatoprotective Effects of Hovenia dulcis Fruit on Ethanol-Induced Liver Damage in vitro and in vivo

2009 ◽  
Vol 38 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Yang-Hee You ◽  
Kuk-Yung Jung ◽  
Yoo-Hyun Lee ◽  
Woo-Jin Jun ◽  
Boo-Yong Lee
Keyword(s):  
Liver Damage ◽  
Hepatoprotective Effects ◽  
Hovenia Dulcis

scholarly journals Hepatoprotective Effects of Extract of Helicteres hirsuta Lour. on Liver Fibrosis Induced by Carbon Tetrachloride in Rats

2021 ◽  
Vol 11 (18) ◽  
pp. 8758
Author(s):  
Dac Thang Hoang ◽  
Thi Thu Hien Truong ◽  
Ngo Viet Duc ◽  
Le Tuan Anh Hoang ◽  
Thi Thao Do ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Fibrosis ◽  
Hepatoprotective Activity ◽  
Oral Medications ◽  
In Vivo Experiments ◽  
Ethanolic Extracts ◽  
Hepatoprotective Effects ◽  
First Time

Helicteres hirsuta Lour. is a traditional Vietnamese medicine for treating chronic liver diseases such as cirrhosis and liver cancer. Many in vitro and in vivo experiments have demonstrated that the extracts and isolated compounds from H. hirsuta have diverse pharmacological activities, including antioxidant, anti-inflammatory, and anti-cancer effects. However, the hepatoprotective effects have not been reported until now. Therefore, the methanolic and ethanolic extracts of the aerial part of the H. hirsuta L. (HHM and HHE-1/1) were examined on liver fibrosis induced by carbon tetrachloride (CCl4) in rats for the first time. The results revealed that all the livers of the model group had stage F4 cirrhosis; the group that received silymarin, and HHM and HHE-1/1 had milder liver damage cirrhosis stage F1-F2 which implies that the methanolic and ethanolic extracts of H. hirsute have a definite advantage in the development of food or oral medications for hepatoprotective activity.

scholarly journals Evaluation of the antioxidant potentials of extract and fractions of Vitex doniana fruit and identification of the bioactive metabolites using HPLC-DAD-MS analysis

2021 ◽  
Vol 17 (1) ◽  
pp. 160-175
Author(s):  
Keziah Uchechi Ajah ◽  
Ozioma Anne Asomugha ◽  
Chinazom Perpetua Ifedigbo ◽  
Kosoluchukwu Chidubem Umeh ◽  
Onyeka Chinwuba Obidiegwu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Carbon Tetrachloride ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Vitex Doniana

Isoflavones and flavonoids in general found in fruits and vegetable act as natural antioxidants. This study was aimed at screening the isoflavone-rich fractions of Vitex doniana fruit extract for antioxidant activity using in vitro and in vivo models. The ethyl acetate and butanol fractions were screened for in vitro antioxidant activity by DPPH free radical scavenging and FRAP (Fe3+ Reducing Antioxidant Power) assays using ascorbic acid as standard. In vivo antioxidant activity was also tested against carbon tetrachloride-induced toxicity in mice using carbon tetrachloride (4 mL/kg) and silymarin (100 mg/kg) as standard. The ethyl acetate fraction and butanol Vacuum Liquid Chromatography (VLC) sub-fractions were subjected to High Performance Liquid Chromatography–Diode Array Detector (HPLC-DAD) and Liquid Chromatography-Mass Spectrometry (LC-MS) analyses. The ethyl acetate and butanol fractions inhibited DPPH radicals with an IC50 of 897 µg/mL and 2809.38 µg/mL respectively. Both fractions also showed mild FRAP result with EC50 of 1401 µg/mL and 7051 µg/mL respectively. The fractions produced significant decrease (P < 0.05) in ALP levels only while ethyl acetate fraction caused a significant reduction in the value of only malondialdehyde. Both fractions also increased levels of superoxide dismutase (SOD). HPLC-DAD analysis led to the detection of cinnamic acid, protocatechuic acid and two isoflavones daidzein and genistein. Further LC-MS analysis confirmed the presence of the isoflavones tectorigenin, 5-O-methylgenistein, and 5-O-methyltectorigenin, among other flavonoids, peonidin, 5-methylpeonidin, methylmalvidin and kaempferol-3-O-(2''galloyl)-glucopyranoside. The detected isoflavones and other flavonoids may contribute to the observed mild in vitro and good in vivo antioxidant activity of V. doniana fruit extract and fractions.

Preparation of Chlorella vulgaris polysaccharides and their antioxidant activity in vitro and in vivo

2019 ◽  
Vol 137 ◽  
pp. 139-150 ◽  
Author(s):  
Mengen Yu ◽  
Mengjiao Chen ◽  
Jiangli Gui ◽  
Shudan Huang ◽  
Yumeng Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorella Vulgaris

scholarly journals EFFECT OF BLUMEA LACERA ON TISSUE GSH, LIPID PEROXIDATION AND HEPATIC CELLS IN ETHANOL INDUCED HEPATOTOXICITY IN RATS

2019 ◽  
pp. 46-50
Author(s):  
DEVENDRA S. SHIRODE ◽  
ASHISH V. KULKARNI ◽  
BRIJENDRA B. JAIN
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Liver Damage ◽  
Ethanol Extract ◽  
Treated Group ◽  
Histopathological Changes ◽  
Hepatic Cells ◽  
Hepatoprotective Effects ◽  
Blumea Lacera

Objective: To evaluate hepatoprotective effects of ethanol extract of aerial part of Blumea lacera (BLEE) against ethanol-induced hepatotoxicity in rats. Methods: The in vivo antioxidant activity of BLEE was assessed by determining the tissue glutathione (GSH) and lipid peroxidation (LPO) levels. The BLEE at the doses of 200 and 400 mg/kg and silymarin 100 mg/kg administered to the ethanol challenged rats. The effects of BLEE and silymarin on Physical and Biochemical Parameters were measured. Similarly, histopathological changes of the liver were studied. Results: The BLEE showed in vivo antioxidant activity. A significant (P<0.001) decrease in SGOT, SGPT, ALP, total and direct bilirubin was observed in BLEE treated group at doses i.e. 200 mg/kg and 400 mg/kg as compared to intoxicated group. Liver damage in animal pretreated with BLEE was minimal with distinct preservation of structures and the architectural frame of the hepatic cells. Conclusion: These findings demonstrated the hepatoprotective effects of BLEE against ethanol-induced liver damage.

Isolation of a novel lutein–protein complex from Chlorella vulgaris and its functional properties

2015 ◽  
Vol 6 (6) ◽  
pp. 1893-1899 ◽  
Author(s):  
Xixi Cai ◽  
Qimin Huang ◽  
Shaoyun Wang
Keyword(s):  
Dietary Supplements ◽  
Functional Properties ◽  
Chlorella Vulgaris ◽  
Protein Complex ◽  
Hepatic Injury ◽  
Radical Scavenging ◽  
Human Beings

The natural lutein–protein complex (LPC) was first purified from Chlorella vulgaris. LPC showed significant radical scavenging effects in vitro and could significantly reduce CCl4-induced hepatic injury in vivo. LPC has the potential for use in making antioxidant dietary supplements for human beings.

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