scholarly journals Influence of extraction method on antioxidant properties of Rheum ribes root extract

2019 ◽  
Vol 30 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Dilek Bilgic Alkaya ◽  
Serap Ayaz Seyhan ◽  
Busra Nagihan Ozturk
Keyword(s):  
Petroleum Ether ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Root Extract ◽  
Anthracene Derivatives

AbstractRheum species are important medicinal herbs, often used in pharmacological research, due to the presence of anthracene derivatives in the subterranean parts of the plant. In this study, we intended to assess its antioxidant capacity, in correlation with the method of extraction. For this purpose, Rheum ribes extraction was realized with four solvents of different polarities (50% methanol, 70% ethanol, 80% acetonitrile, and petroleum ether). We used different extraction techniques, such as orbital shaker, ultrasonic stirrer, microwave, and Soxhlet extraction, and the total phenolic content of the Rheum ribes extracts was determined by modified Folin–Ciocalteu method. The reducing power and radical scavenging activity of the extracts were also evaluated. The results shown that the antioxidant activity of the extracts depends on the extraction methods especially through the used solvent and decreases in the order: ethanol > methanol > acetonitrile > petroleum ether.

scholarly journals Strobilanthes heyneanus root extract as a potential source for antioxidant and antimicrobial activity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Vijayakumar Sundaram ◽  
Selvaraju Sadhasivam ◽  
Sivaraj Chandrasekaran ◽  
Raaman Nanjian ◽  
Arjun Pandian
Keyword(s):  
Dermal Fibroblast ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Fibroblast Cell ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Scavenging Activity ◽  
Medicinal Uses

Abstract Background Strobilanthes heyneanus (Nilgirianthus heyneanus) belongs to the family Acanthaceae that contains many species with potential for diverse medicinal uses. It is also called ‘Karun kurinji’ and is commonly found in the South-West regions of India. The species are commonly used in rheumatic complaints, sprain of the ankle, and hernia. The objectives of the study were to evaluate the antioxidant activity, phytochemical analysis, and antibacterial activities of the root extract of S. heyneanus. Results The radical scavenging and reduction assays such as DPPH● radical and OH● radical scavenging assays, as well as phosphomolybdenum reduction and Fe3+ reducing power assays, were determined for the root extract. The highest DPPH● radical scavenging activity was 88.23 ± 1.32 at 120 μg/mL concentration, and the calculated IC50 was 38.52 μg/mL concentration. The highest OH● radical scavenging activity was 51.28 ± 1.06 at 120 μg/mL concentration, and the calculated IC50 was 51.28 μg/mL concentration. The highest ABTS●+ radical scavenging activity was 91.28 ± 1.12 at 30 μg/mL concentration, and the calculated IC50 was 33.92 μg/mL concentration. The highest phosphomolybdenum reduction was 87.43 ± 0.90 at 120 μg/mL concentration, and the calculated RC50 was 24.74 μg/mL concentration. The highest Fe3+ reduction was 89.38 ± 0.98 at 120 μg/mL concentration, and the calculated RC50 was 31.06 μg/mL concentration. The antibacterial activity of S. heyneanus showed the highest zone of inhibition of 24 mm for Salmonella typhi with 500 μg/mL concentration. The cytotoxicity limits of the root extracts were tested by MTT assay using human dermal fibroblast cell lines, reflecting > 90% cell viability at a concentration of 500 μg/mL. Conclusions The current study showed that the root extract of S. heyneanus has better antioxidant properties and potential anti-bacterial compounds. The phytochemical analysis of the root extract showed the presence of alkaloids, steroids, terpenoids, phenols, flavonoids, tannins, saponins, glycosides, and carbohydrates, which are responsible for the antibacterial root extract of S. heyneanus synergistically.

scholarly journals Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

2017 ◽  
Vol 71 (5) ◽  
pp. 361-370
Author(s):  
Slavica Grujic ◽  
Ana Dzamic ◽  
Violeta Mitic ◽  
Vesna Stankov-Jovanovic ◽  
P.D. Marin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

The Effects of Solvents and Extraction Methods on the Antioxidant Activity of P. niruri

2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hafizah Mohd Hadzri ◽  
Mohd Azizi Che Yunus ◽  
Salman Zhari ◽  
Fahim Rithwan
Keyword(s):  
Antioxidant Activity ◽  
Extraction Method ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Solvent Polarity ◽  
Extraction Methods ◽  
The Other ◽  
Natural Antioxidant ◽  
Total Phenolic

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.

scholarly journals A Comparative Study on Antioxidant Properties of Two Phyllanthus Species Growing in Bangladesh

2013 ◽  
Vol 11 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Ishrat Nimmi ◽  
Ismet Ara Jahan ◽  
M Hemayet Hossain ◽  
Mohammad Burhan Uddin ◽  
M Sohel Rana ◽  
...  
Keyword(s):  
Comparative Study ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Test Methods ◽  
Total Phenolic ◽  
Solvent Volume

A comparative study of antioxidant properties on two varieties of Phyllanthus (Phyllanthus niruiri L. & Phyllanthus urinaria L. leaves) growing in Bangladesh was done in the present study. Five complimentary test methods namely DPPH free radical scavenging activity, reducing power assay, total antioxidant capacity, total phenolic and flavonoid contents determination were used for the evaluation of antioxidant properties of two species of Phyllanthus. In the present investigation for the evaluation of antioxidant activities the concentration is expressed by the ratio of crude sample per solvent volume instead of extract weight per solvent volume. Based on these concentrations the investigated P. niruri L. and P. urinaria L. leaves extracts exhibited significant results. At 2 mg/ml concentration DPPH radical scavenging capacity of the methanol extract of both the plants was found to show significant (>90%) activity which is comparable to Ascorbic acid and BHA. In case of reducing power tests for both the extract, the activity of P. urinaria is comparable to that of BHA and AA and that of P. niruiri is comparable to BHA. P. niruiri showed better antioxidant potential than that of P. urinaria based on the observed results of their corresponding methanol extract. DOI: http://dx.doi.org/10.3329/dujps.v11i2.14579 Dhaka Univ. J. Pharm. Sci. 11(2): 191-197, 2012 (December)

scholarly journals Antioxidant Capacities of Jujube Fruit Seeds and Peel Pulp

2020 ◽  
Vol 10 (17) ◽  
pp. 6007 ◽  
Author(s):  
Yung-Sheng Lin ◽  
Wen-Shin Lin ◽  
Jing-Wen Tung ◽  
Ya-Chih Cheng ◽  
Min-Yun Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Jujube Fruit

In this study, the effects of different fruit parts and extraction conditions on the antioxidant properties of jujube (Ziziphus jujuba Mill.) fruit were investigated. Five in vitro antioxidant models and statistical analyses were performed. The results revealed that jujube peel with pulp (peel pulp) exhibited better antioxidant capacity than did seeds. Overall, jujube peel pulp extracted using 50% ethanol at 60 °C exhibited the best antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (0.3 ± 0 mg/mL), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity (0.5 ± 0 mg/mL), total phenolic content (38.3 ± 0.4 mg gallic acid equivalent per gram dry weight), total flavonoid content (43.8 ± 0.2 mg quercetin equivalent per gram dry weight), and reducing power (41.9 ± 2.2 mg ascorbic acid equivalent per gram dry weight). The results indicated that jujube peel pulp is a more potential natural antioxidant than seeds.

scholarly journals The Antioxidant Activity of Maltese Honey

2014 ◽  
Vol 58 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Natacha Meinen ◽  
Liberato Camilleri ◽  
Everaldo Attard
Keyword(s):  
Principal Components ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Ancient Greek ◽  
Colour Intensity ◽  
Polyphenol Content ◽  
Ancient Times

Abstract Maltese honey has been valued for its medicinal properties since ancient times, so much so that the word Malta is derived from the ancient Greek word, Melite, which means honey. In this study, Maltese honey samples (n = 30) from three harvest seasons and different floral sources were analysed to determine their antioxidant/antiradical properties. Colorimetric assays were used to determine the polyphenol content, flavonoid content, and colour intensity, and these values were analysed to determine whether they correlated with radical scavenging activity as determined by bioassays. The honey samples varied in their DPPH activity (range, 3.326 - 11.865 mg AEAC/100 g honey), reducing power (EC 50 range: 5.632 - 14.453 mg/ml), total phenolic content (range, 8.114 - 109.765 mg TAE/100 g honey), total flavonoids (range, 0.655 - 212.865 mg RE/100 g honey), and colour intensity (range, 0.000 - 99.581 mm Pfund). The data were subjected to factor analysis using principal components. The antioxidant properties correlated significantly with total polyphenol content and colour (p<0.0001). Principal components analysis revealed three clusters of honey corresponding to the three harvest seasons.

scholarly journals Four Different Methods Comparison for Extraction of Astaxanthin from Green AlgaHaematococcus pluvialis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Shengzhao Dong ◽  
Yi Huang ◽  
Rui Zhang ◽  
Shihui Wang ◽  
Yun Liu
Keyword(s):  
Fatty Acid ◽  
Haematococcus Pluvialis ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Major Fatty Acid ◽  
Acid Pretreatment ◽  
Acetone Extraction

Haematococcus pluvialisis one of the potent organisms for production of astaxanthin. Up to now, no efficient method has been achieved due to its thick cell wall hindering solvent extraction of astaxanthin. In this study, four different methods, hydrochloric acid pretreatment followed by acetone extraction (HCl-ACE), hexane/isopropanol (6 : 4, v/v) mixture solvents extraction (HEX-IPA), methanol extraction followed by acetone extraction (MET-ACE, 2-step extraction), and soy-oil extraction, were intensively evaluated for extraction of astaxanthin fromH. pluvialis. Results showed that HCl-ACE method could obtain the highest oil yield(33.3±1.1%)and astaxanthin content(19.8±1.1%). Quantitative NMR analysis provided the fatty acid chain profiles of total lipid extracts. In all cases, oleyl chains were predominant, and high amounts of polyunsaturated fatty acid chains were observed and the major fatty acid components were oleic acid (13–35%), linoleic acid (37–43%), linolenic acid (20–31%), and total saturated acid (17–28%). DPPH radical scavenging activity of extract obtained by HCl-ACE was73.2±1.0%, which is the highest amongst the four methods. The reducing power of extract obtained by four extraction methods was also examined. It was concluded that the proposed extraction method of HCl-ACE in this work allowed efficient astaxanthin extractability with high antioxidant properties.

A COMPARATIVE STUDY OF IN VITRO ANTIOXIDANT POTENTIAL, PHOTOPROTECTIVE SCREENING OF EHRETIA ACUMINATA R.BR. LEAVES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (09) ◽  
pp. 30-36
Author(s):  
A Shukla ◽  
A. Kaur ◽  
R. K Shukla ◽  
Anchal ◽  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Protective Factor ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Synthetic Drugs

To evaluate the comparability of some of the most common radical scavenging assays using 2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, Ferric reducing power assay (FRAP) & nitric oxide. leaves of traditionally important medicinal plant Ehretia acuminata, used in Chinese herbal medicine were identified & analyzed for their antioxidant capacity, total phenolic and flavonoid content & sun protective factor (SPF). In this study different organic solvents, petroleum ether 40-600 C (PE), chloroform (CH), ethyl acetate (EA), ethanol 99.9% (EOL) & water (AQ) were used for soxhlet exraction of E. acuminata leaves. Among them, ethyl acetate extract exhibited strongest antioxidant activity with IC50 between 99 to 250 μg/ml. Ascorbic acid (AA) used as a standard antioxidant exhibited IC50 25µg/ml. Petroleum ether showed weak free radical scavenging activity with IC50 value between 500 to 770 µg/ml. The ability of protection against UV-B region followed the trend EOL> EA> PE> AQ> CH extract. The result reveals that these natural antioxidant potential of E. acuminata represents a side effect-free alternative to synthetic antioxidants in the food processing industry, in sunscreen formulations and for use in preventive synthetic drugs

scholarly journals A mechanistic approach to anti-nociceptive potential of Nymphaea lotus Linn (Nymphaeaceae) in rodents

2020 ◽  
Vol 9 (5) ◽  
pp. 291-299
Author(s):  
E.O Agbaje ◽  
◽  
A Iwe ◽  
OJ Oguntokun ◽  
◽  
...  
Keyword(s):  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
K Channel ◽  
Total Phenolic ◽  
Hot Plate ◽  
Adrenergic Systems ◽  
Tail Clip

Background: Nymphaea lotus Linn. (Nymphaeaceae), commonly known as white water-lily, white lotus or Egyptian lotus, is an important and well-known medicinal plant, widely used in the Ayurveda and Siddha systems of medicine for the treatment of diabetes, inflammation, liver disorders, urinary disorders, fever, skin diseases, cancer, gonorrhoea, pain and bronchitis. Objective: The study was designed to explore anti-nociceptive potential of aqueous extract of Nymphaea lotus leaf, its possible mechanism of action, and antioxidant properties. Methods: The anti-nociceptive activity of Nymphaea lotus (50, 100 and 250 mg/kg) was explored using writhing, formalin, tail clip and hot plate tests, while formalin test was used to investigate the involvement of opioid, dopamine, serotonin, K+ channel blocker, α1-adrenergic and α2- adrenergic systems. The antioxidant effect was carried out using DPPH, nitric oxide free radical scavenging activity and the reducing power effect. Total phenolic and flavonoids contents were also explored. Results: Oral administration of N. lotus in doses of 50, 100 and 250 mg/kg recorded a significant (p<0.05) dose dependent obstruction of nociception. A remarkable effect was recorded with the writhing and formalin tests and a significant effect was also observed in the tail clip and hot plate test, which suggests peripheral and central anti-nociceptive activity of the extract. The anti-nociceptive effect produced by N. lotus was significantly reversed by naloxone and yohimbine, suggesting the possible involvement of opioid and α2–adrenergic systems in its anti-nociceptive activity. N. lotus also displayed a potent antioxidant activity. Conclusion: These findings justify the folkloric use of N. lotus in pain management.

scholarly journals Bio-Prospecting of a Few Brown Seaweeds for Their Cytotoxic and Antioxidant Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Rashmi C. Vinayak ◽  
A. S. Sabu ◽  
Anil Chatterji
Keyword(s):  
Metal Ion ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Brown Seaweeds ◽  
Methanolic Extracts

Methanolic extracts (MEs) of seven brown seaweeds occurring in the Indian coastal waters were screened for their cytotoxic and antioxidant properties following various assays. The methanolic extracts of seaweeds in the order ofDictyopteris australis > Spatoglossum variabile > Stoechospermum marginatum > Spatoglossum aspermumshowed significant cytotoxic activity. A very high DPPH radical scavenging activity was exhibited by the methanolic extracts prepared fromSt. marginatum, Padina tetrastromatica, Dictyopteris delicatulaandS. aspermum. The total phenolic content of the MEs varied from 13.19 ± 0.32 to 25.29 ± 0.445 gallic acid equivalents (mg g−1of methanolic extract). The reducing power assay indicated a dose dependency, at concentrations of 0.1, 0.5 and 1.0 and 2.0 mg mL−1of MEs and decreased in the following order:Butylated hydroxy toluene > P. tetrastromatica > D. delicatula > S. aspermum > S. variabile > S. marginatum > D. australis > S. marginatum. Furthermore,D. australis,S. aspermum, S. variabileandS. marginatumdemonstrated good metal ion chelating properties. All the above evidences suggest that, the antioxidant compounds found in brown seaweeds scavenge free radicals through effective intervention. This decisively promotes them as a potential source of natural antioxidants.

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